BIOSENSORS & BIOELECTRONICS
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2019
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IF 9.518
Qia Wang,Yuting Zhao,Qingui Yang,Dan Du,Haipeng Yang,Yuehe Lin
Amperometric enzyme biosensors are some of the simplest and cheapest types of medical devices used in the rapid detection of biomarkers that have been developed in the past fifty years. When the concentrations of biomarkers are at micromoles per liter, such as for sarcosine , which was recently discovered as a biomarker for prostate cancer, the response signal of the interferences is huge, and the biosensor is hard to satisfy the requirements of practical applications. In this manuscript, we describe a strategy for synthesizing a surface electronegative organic-inorganic hybrid mesoporous material , which could reduce the interference signal much better than Nafion and Chitosan. We verify that the surface potential of the carrier nanomaterial plays an important role in excluding anionic interferences. We also prepare a sensitive (16.35 μA mM −1 ), low LOD (0.13 μM) and wide linear range (1–70 μM) amperometric sarcosine biosensor with excellent anti-interference properties. This mesoporous material provides a bio-composite platform for the development of simple amperometric biosensors for detecting micromoles per liter of analytes in serum or urine.
研究领域:
生物医学工程
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分析化学
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癌症诊断
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产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2019
·Vol. 30
·期 48
·485706
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IF 3.399
Feng Long,Wei Li,Wanling Chen,Danni Liu,Yue Chen,Rui Zhou,Penghui Li
In this work, a novel label-free electrochemical biosensor is developed for the detection of galectin-1 (Gal-1) based on gold nanoparticle (AuNP) loaded octahedral Cu2O (Cu2O@Au) nanocomposites. The AuNPs on the surface of the Cu2O nanocrystals not only enhance the electrochemical performance, but also serve as the binding sites for the lactose ligand which can specifically bind with Gal-1. The Cu2O@Au nanocomposites provide the synergic effect of electrochemical signal amplification and lactose–galectin reaction as the recognition strategy. Under optimal conditions, the proposed biosensor exhibits a variation of electrochemical responses to different concentrations of Gal-1 ranging from 0.1 pg ml−1 to 10 ng ml−1. This work presents an alternative electrochemical biosensor for the detection of tumor biomarkers based on a simple and economical lactose ligand incorporated Cu2O@Au biosensor platform.
研究领域:
生物传感器
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分析化学
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癌症研究
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
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2015
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IF 3.545
Huifang Hu,Miao Feng,Hongbing Zhan
An amperometric glucose biosensor based on direct electron transfer of glucose oxidase (GOD) self-assembled on the surface of partially unzipped carbon nanotubes (PUCNTs) modified glassy carbon electrode (GCE) has been successfully fabricated. PUCNTs were synthesized via a facile chemical oxidative etching CNTs and used as a novel immobilization matrix for GOD. The cyclic voltammetric result of the PUCNT/GOD/GCE showed a pair of well-defined and quasi-reversible redox peaks with a formal potential of −0.470 V and a peak to peak separation of 37 mV, revealing that the fast direct electron transfer between GOD and the electrode has been achieved. It is notable that the glucose determination has been achieved in mediator-free condition. The developed biosensor displayed satisfactory analytical performance toward glucose including high sensitivity (19.50 μA mM −1 cm −2 ), low apparent Michaelis–Menten (5.09 mM), a wide linear range of 0–17 mM, and also preventing the interference from ascorbic acid , uric acid and dopamine usually coexisting with glucose in human blood. In addition, the biosensor acquired excellent storage stabilities. This facile, fast, environment-friendly and economical preparation strategy of PUCNT–GOD may provide a new platform for the fabrication of biocompatible glucose biosensors and other types of biosensors.
研究领域:
生物传感器
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纳米技术
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电化学
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Bing Zhang,Chuanmin Ding
The sensitive determination of carcino embryonie antigen (CEA)-a set of highly related glycoproteins involved in cell adhesion-is beneficial to the early diagnosis of colorectal cancer. In this study, a novel displacement-type amperometric immunosensing platform, based on the fact that glucose and Alizarin Red S (ARS) compete for phenylboronic acid bindng sites, was projected for sensitive detection of tumour marker (CEA, used as a model) on a PAMAM dendrimer-encapsulated nanogold (PAMAM-Au)-functionlized sensing interface. Firstly, 4 − mercaptophenylboronic acid (S-PBA) was assembled onto the PAMAM-Au via the S-Au interaction, and then ARS was immobilized by S-PBA binding of cis-diol moieties, which was dropped on the surface of glassy carbon electrode as sensing platform to combine antibody. Meanwhile, amylase modified gold nanoparticles were employed as labels. Accompanying the sandwich immunoassay , the carried amylase could hydrolyze amylose into glucose, and the displacement-type format was triggered due to the stronger adhesion between glucose and PBA, which resulted in the change of electrochemical signals due to the decrease of ARS (as an electron mediator). Under optimal conditions, the SWV signals were related to the concentration of CEA, indicating a certain proportional relation in a range of 0.01~50 ng mL −1 with a detection limit (LOD) of 0.003 ng mL −1 . Intra- and inter-assay coefficients of variation were below 10%, respectively. In addition, the methodology showed good accordance with a commercialized enzyme-linked immunosorbent assay (ELISA) method.
研究领域:
生物化学
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分析化学
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癌症诊断
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电化学传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
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IF 8
Wenwen Chen,Jie Zou,Liang Shan,Huanhuan Zhang,Wentian Wang,Jiawen Jian,Tao Yuan,Xin Zhang,Yucun Zhou
Selective catalytic reduction (SCR) utilizing urea hydrolysis to generate ammonia (NH 3 ) is the most efficient technology to reduce the emission of nitrogen oxides (NO x ). NH 3 detection is crucial in the closed-loop feedback system for the precise urea injection. In this work, a high-temperature NH 3 sensor using a BaZr 0.8 Y 0.2 O 3-δ (BZY) proton conductor as the electrolyte and a BaCo 0.4 Fe 0.4 Zr 0.1 Y 0.1 O 3-δ (BCFZY) H + /e – /O 2– triple conductor as the sensing electrode (SE) is reported. The effect of the micromorphology of the BCFZY SE on the sensing performance is investigated by varying the sintering temperature of SE. The sensor shows high sensitivity to NH 3 with response currents of 0.39, 1.31, 3.03, and 1.81 μA/ppm at 350, 400, 450, and 500 °C, respectively. Furthermore, the sensor shows excellent selectivity and repeatability. This work demonstrates that the BCFZY based sensor has great potential for the high-temperature NH 3 detection.
研究领域:
材料科学
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电化学
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环境工程
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传感器与执行器
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）