SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Xuemeng Li,Jinxiu Wei,Kai Zhu,Yuanyuan Liu,Lei Wu,Kuo Yang,Shenfei Zong,Zhuyuan Wang,Yiping Cui
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) caused the global pandemic known as coronavirus disease 2019 (COVID-19). Accurate screening of SARS-CoV-2 is crucial in controlling the spread of the virus. Moreover, the false positive diagnosis will cause significant waste of the medical resources. Hence, we introduce a s uper- r esolution d ual f luorescence c o-localization (SR-DFC) strategy for the detection of SARS-CoV-2, which can greatly reduce false positive rates. In the SR-DFC assay, fluorescent capture probes are first modified onto periodically structured gold array substrates to capture the spike protein of SARS-CoV-2. Then, fluorescent recognition probes are added to identify SARS-CoV-2, forming sandwich-like immune complex structures. SR-DFC is realized by utilizing single-molecule localization microscopy (SMLM). Specific recognition of SARS-CoV-2 will induce high fluorescence colocalization between the capture probes and recognition probes. Owing to the high spatial resolution of SMLM, nonspecific adsorption can be well excluded, which greatly improves the specificity and significantly reduces false positive rates. Using a pixel counting method proposed by our group, quantitative detection of SARS-CoV-2 could be realized. The detection range covered in this study is between 0.1 ng/mL to 1000 ng/mL. By simply changing capture probes and recognition probes, the SR-DFC strategy is feasible for the detection of other targets, indicating a good universality. Such a highly accurate sensing strategy holds promising potential in the screening of infectious diseases.
研究领域:
病毒学
·
医学诊断
·
显微镜技术
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Xupeng Wang,Bing Gong,Erkai He,Willie J.G.M. Peijnenburg,Hao Qiu
Awareness of toxicokinetics at the subcellular level is crucial to deciphering the underlying intoxication processes of metal(loid)s, although this information is often lacking. Here, the toxicokinetics of two non-essential metal(loid)s (Cd and As) and one essential metal (Zn) in both the whole body and subcellular fractions of earthworm ( Eisenia fetida ) were assessed. Earthworms were exposed to natural soils originating from a gradient of metal(loid) pollution for 14 days followed by a 14-day elimination phase in clean soil. Clearly distinct toxicokinetic patterns were found in the earthworms according to the metal(loid) considered. An obvious concentration-dependent increase was observed in earthworms or subcellular compartments where no equilibrium was reached (with slow or no elimination) for Cd and As throughout the experiment. As for Zn, the earthworms were able to retain a steady-state concentration of Zn in its body or each fraction without a clear intake behavior via the dynamic trade-off between uptake and elimination at different pollution levels. These differences in toxicokinetics at the subcellular level supported the observed differences in bioaccumulation patterns and were indicative of the strategy by which non-essential and essential elements are handled by earthworms. Notably, the concentration of Cd and As in subcellular compartments showed the same pattern as for Zn in the order of cellular cytosol > cellular debris > metal-rich granules, which might be associated with the binding of non-essential/essential elements with metallothionein enriched in the cytosol. Our findings enhance the understanding of the underlying mechanisms for metal(loid) accumulation kinetics in earthworms from the perspective of subcellular partitioning, and will be beneficial for accurate risk assessment of Cd, As, and Zn.
研究领域:
环境科学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2019
·Vol. 76
·期 5
·1920-1928
·
IF 3.255
Min Li,Yixin Yang,Yonghong Yao,Weifang Xiang,Jiayi Han,Yonghui Wang,Penghua Bai,Jing Wang,Gengping Zhu,Liang Man,Fan Zhang,Lina Pan
BACKGROUND Chouioia cunea Yang (Hymenoptera: Eulophidae) is a parasitic wasp and natural enemy of several lepidopteran pests during their pupal stage. The volatiles from pupae of three hosts, Hyphantria cunea (Arctiidae), Antheraea pernyi (Saturniidae) and Lymantria dispar (Erebidae), were analyzed and compared to elucidate the chemical cues used by C. cunea to locate its hosts. RESULTS The attraction of C. cunea to H. cunea pupae has no obvious association with the types of plant leaves consumed by H. cunea before pupation. C. cunea exhibited the strongest attraction to the pupae of H. cunea , followed by those of A. pernyi and L. dispar based on behavioral experiments. Gas chromatography-mass spectrometry and GC-electroantennography (GC-EAD) analyses showed that these three host pupae consisted of essentially the same active volatile components but at different relative amounts. Active components derived from these pupae by GC-EAD were alkanes from C12 to C27, and C. cunea showed different levels of attraction to different single compounds. CONCLUSION Host location by C. cunea primarily depends on common compounds emanating from the pupae of several host species. The relative amount of each component varies across host species, guiding host preferences by C. cunea . Optimal blends of several components were identified. Understanding the chemical cues used by C. cunea to locate its host could increase the possibility of developing attractants for parasitic wasps and subsequently increasing the parasitism rate of C. cunea on various hosts. © 2019 Society of Chemical Industry
研究领域:
昆虫学
·
化学生态学
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-12
·
IF 4.259
Zhang Chong,Cao Songxiao,Jin Yazhong,Ju Lijun,Chen Qiang,Xing Qiaojuan,Qi Hongyan
To better understand the function role of the melon CmLOX18 gene in the biosynthesis of C6 volatiles during fruit ripening, we biochemically characterized CmLOX18 and identified its subcellular localization in transgenic tomato plants. Heterologous expression in yeast cells showed that the molecular weight of the CmLOX18 protein was identical to that predicted, and that this enzyme possesseed lipoxygenase activity. Linoleic acid was demonstrated to be the preferred substrate for the purified recombinant CmLOX18 protein, which exhibited optimal catalytic activity at pH 4.5 and 30 °C. Chromatogram analysis of the reaction product indicated that the CmLOX18 protein exhibited positional specificity, as evidenced by its release of only a C-13 oxidized product. Subcellular localization analysis by transient expression in Arabidopsis protoplasts showed that CmLOX18 was localized to non-chloroplast organelles. When the CmLOX18 gene was transgenically expressed in tomato via Agrobacterium tumefaciens -mediated transformation, it was shown to enhance expression levels of the tomato hydroperoxide lyase gene LeHPL , whereas the expression levels of six TomLox genes were little changed. Furthermore, transgenic tomato fruits exhibited increases in the content of the C6 volatiles, namely hexanal, (Z)-3-hexanal, and (Z)-3-hexen-1-ol, indicating that CmLOX18 probably plays an important role in the synthesis of C6 compounds in fruits.
研究领域:
植物学
·
生物化学
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT PHYSIOLOGY
·
2024
·Vol. 197
·期 1
·
IF 6.5
Song Ru-Feng,Liao Cai-Yi,Wang Lin-Feng,Lu Kai-Kai,Zhang Chi,Wu Run-Xin,Wu Ji-Xiao,Ma Yu-Qing,Kuang Lei,Guo Ning,Yuan Hong-Mei,Liu Wen-Cheng
The plasma membrane (PM)-localized Na+/H+ antiporter Salt Overly Sensitive1 (SOS1) is essential for plant salt tolerance through facilitating Na+ efflux; however, how SOS1 localization and protein accumulation is regulated in plants remains elusive. Here, we report that Sorting Nexin 1 (SNX1) is required for plant salt-stress tolerance through affecting endosomal trafficking of SOS1 in Arabidopsis (Arabidopsis thaliana). Disruption of SNX1 caused salt hypersensitivity with increased Na+ accumulation and decreased Na+ efflux in Arabidopsis when challenged with high salinity stress. SNX1 co-localized and interacted with SOS1 in endosomes, promoting its PM localization and protein stability in plants under saline conditions. SOS1 overexpression promoted salt tolerance in the wild-type, whereas such effect was greatly compromised in the snx1-2 mutant. Pharmaceutical results showed that SOS1 recycling from the cytosol to the PM was largely blocked while its vacuolar degradation was accelerated in the snx1-2 mutant. Furthermore, salt-induced SOS1 phosphorylation enhanced its interaction and co-localization with SNX1, which is required for SOS1 PM localization in plants. Our study elucidates that SNX1 facilitates SOS1 PM localization and protein accumulation through endosomal trafficking, thereby enhancing salt tolerance in plants.
研究领域:
植物学
·
分子生物学
✓ 已验证
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 3.7
Guangjuan Luo,Ying Lin,Shupeng Ruan,Ruiming Xiao,Xinying Zhang,Shuli Liang
ABSTRACT Mitochondria are a potential sub-organelle for the synthesis of terpenoids due to its abundant acetyl-CoA, cofactors, and compact compartment for targeting metabolic pathways and enzymes. Hence, conducting systematic investigations and harnessing the subcellular compartment of mitochondria may offer a viable avenue for enhancing terpenoids biosynthesis in yeast and other eukaryotic cells. Previous endeavors to boost terpenoids production in Komagataella phaffii mainly focused on diverse metabolic engineering strategies in the cytoplasm. Numerous studies have demonstrated that reconstructing specific metabolic pathways within mitochondria can lead to higher production yields than engineering the same pathways in the cytoplasm. In our study, we initially identified 6 well-performed mitochondrial targeting sequences (MTSs) through colocalization observation of EGFP and a mitochondrial red fluorescent probe via fluorescence microscopy. These MTSs were selected for the assembly of complete patchoulol biosynthesis pathways in the mitochondria, which resulted in a 1.7-fold increase in patchoulol production compared to the original strain. Furthermore, dual regulation of the cytoplasm and mitochondria was implemented in the P6H4＋PM2 strain to further enhance patchoulol synthesis to 109 mg/L, representing a 1.0-fold and 10.7-fold greater yield than that of the strains engineered solely in the cytoplasm or mitochondria, respectively. This study offers valuable guidance for effectively utilizing mitochondria to achieve higher patchoulol and other sesquiterpenes through dual cytoplasmic-mitochondrial engineering in K. phaffii .
研究领域:
生物化学
·
合成生物学
✓ 已验证
产品关联
DOI
高影响因子
NATURE
·
2023
·Vol. 622
·期 7983
·493-498
·
IF 64.8
Han Dongyuan,Wang Jie,Agosta Lorenzo,Zang Ziang,Zhao Bin,Kong Lingmei,Lu Haizhou,Mosquera-Lois Irea,Carnevali Virginia,Dong Jianchao,Zhou Jianheng,Ji Huiyu,Pfeifer Lukas,Zakeeruddin Shaik M.,Yang Yingguo,Wu Bo,Rothlisberger Ursula,Yang Xuyong,Grätzel Michael,Wang Ning
Lead halide perovskite light-emitting diodes (PeLEDs) have demonstrated remarkable optoelectronic performance 1 , 2 , 3 . However, there are potential toxicity issues with lead 4 , 5 and removing lead from the best-performing PeLEDs—without compromising their high external quantum efficiencies—remains a challenge. Here we report a tautomeric-mixture-coordination-induced electron localization strategy to stabilize the lead-free tin perovskite TEA 2 SnI 4 (TEAI is 2-thiopheneethylammonium iodide) by incorporating cyanuric acid. We demonstrate that a crucial function of the coordination is to amplify the electronic effects, even for those Sn atoms that aren’t strongly bonded with cyanuric acid owing to the formation of hydrogen-bonded tautomeric dimer and trimer superstructures on the perovskite surface. This electron localization weakens adverse effects from Anderson localization and improves ordering in the crystal structure of TEA 2 SnI 4 . These factors result in a two-orders-of-magnitude reduction in the non-radiative recombination capture coefficient and an approximately twofold enhancement in the exciton binding energy. Our lead-free PeLED has an external quantum efficiency of up to 20.29%, representing a performance comparable to that of state-of-the-art lead-containing PeLEDs 6 , 7 , 8 , 9 , 10 , 11 , 12 . We anticipate that these findings will provide insights into the stabilization of Sn(II) perovskites and further the development of lead-free perovskite applications.
研究领域:
材料科学
·
光电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 410
·期 2
·585-594
·
IF 3.431
Zhang Jing,Liang Lijia,Guan Xin,Deng Rong,Qu Huixin,Huang Dianshuai,Xu Shuping,Liang Chongyang,Xu Weiqing
A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) probe (5-ethynyl-2′-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles.
A universal strategy was developed to accurately locate the nuclear region and obtain precise molecular information of cell nuclei by SERS
研究领域:
癌症研究
·
分子生物学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-14
·
IF 4.122
Huang Wenli,Jiao Jie,Ru Mei,Bai Zhenqing,Yuan Honglin,Bao Zhian,Liang Zongsuo
Coptis chinensis Franch. is one of the most important medicinal plants globally. However, this species contains relatively high concentrations of chromium (Cr) which potentially detrimental to human health. It is important to understand Cr localization and speciation in order to evaluate its accumulation and transportation mechanisms and minimize Cr transfer to humans. As little previous work in this area has been carried out, we utilized synchrotron radiation microscopic X-ray fluorescence (SR-μXRF) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to spatially locate Cr, X-ray absorption near-edge spectroscopy (XANES) to analyze Cr speciation, and inductively coupled plasma mass spectrometry (ICP-MS) to detect Cr subcellular concentration. Micromapping results showed that Cr was distributed predominantly within the vascular cylinder, the periderm and some outer cortex, and the cortex and some vascular bundles in root, rhizome, and petiole, respectively. XANES data showed that Cr(VI) can be reduced to Cr(III) when grown with Cr(VI), and yielded a novel conclusion that this plant contain elemental chromium. ICP-MS data showed that Cr was primarily compartmentalized in cell walls in all tissues. The new insights on Cr accumulation in C. chinensis Franch. provide a theoretical basis for the evaluation of Cr in other medicinal plants.
研究领域:
植物学
·
环境科学
·
药用植物研究
✓ 已验证
DOI
高影响因子
Nano-Micro Letters
·
2024
·Vol. 16
·期 1
·1-19
·
IF 31.6
Zhao Xiaomeng,Zhang Heng,Chan Kit-Ying,Huang Xinyue,Yang Yunfei,Shen Xi
Inspired by transport system in trees, a two-way water and salt transport mechanism is realized in a structurally graded aerogel, enabling simultaneous fast water uptake and salt rejection.
The horizontally aligned pore channels near the surface achieve excellent heat localization by maximizing solar absorption and minimizing heat loss.
The integrated water, salt, and thermal transports impart an impressive evaporation rate of 1.94 kg m −2 h −1 in a 20 wt% NaCl solution for 8 h without salt accumulation.
研究领域:
材料科学
·
能源工程
·
仿生学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Lulu Zhang,Runye Liu,Luyao Liu,Xiaoxing Xing,Haoyuan Cai,Yongdong Fu,Jianhai Sun,Wang Ruan,Jian Chen,Xianbo Qiu,Duli Yu
The investigation of the interactions between cells and drugs forms a crucial aspect of biological and clinical medical studies. Generally, single-cell or local-cellular studies require a microscopic imaging system with high magnifications, which suffers from low detection throughputs and poor time responses. The study presented in this paper combined SPR and fluorescence to achieve cell localization, real-time monitoring of cell images and quantitative analysis of drugs. In order to obtain more comprehensive, accurate and real-time data, a dual-mode system based on surface plasmon resonance (SPR) and fluorescence was constructed based on a 4× magnification lens. This enables simultaneous studies of an entire cell and a specific region of the cell membrane. An adaptive adjustment algorithm was established for distorted SPR images, achieving temporal and spatial matching of the dual-mode detection. The combination of SPR and fluorescence not only achieved micro-detection but also complemented the qualitative or quantitative limitations of SPR or fluorescence method alone. In system characterization, the response signal of SPR was noticed to increase with the increasing concentration of EGF in stimulated cells. It indicated that this platform could be employed for quantitative detection of the cell membrane region. Upon addition of EGF, a peak in the SPR curve was observed, and the cells in the corresponding SPR image turned whiter. This indicated that the platform can simultaneously monitor the SPR response signal and image changes. The response time of fluorescence in EGF testing was several seconds earlier than SPR, revealing that signal transduction first occurred in the whole cell and then propagated to the cell membrane region. The inhibitory ability of Gefitinib on cells was verified in a fast and real-time manner within 20 min. The results indicated that the detection limit of this method was 20 IU/mL for EGF and 10 µg/mL for Gefitinib. In conclusion, this study demonstrates the advantages of SPR and fluorescence dual-mode techniques in the analysis of cell-drug interactions, as well as their strong potential in drug screening.
研究领域:
生物医学工程
·
药物研究
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Mengjiao Yang,Yingjie Xu,Xiaozhou Xin,Chengjun Zeng,Yuanshi Li,Cheng Lin,Yanju Liu,Yuanyuan Guo,Jinsong Leng
Congenital heart defects (CHDs) are one of the most common congenital malformations, accounting for ≈30% of all congenital malformations. Interventional implantation of occlusion devices is becoming the preferred treatment for CHDs. However, current occlusion devices suffer from serious problems, such as thrombosis, slow endothelialization, imprecise localization, abrasion, displacement, etc. Here, a multifunctional drug-carrying fiber platform with structural similar to the extracellular matrix is innovatively designed to develop 4D printed cardiac occlusion devices, with characteristics of efficient anticoagulation, proendothelialization, and precise localization. Biomimetic ligament structures are designed to achieve a similar mechanical response to myocardial tissue, which helps to synergize deformation and reduce tissue wear. A structural design method for biomimetic personalized multilevel occlusion devices is proposed, facilitating further improvement of sealing reliability. The radiopaque 4D printed shape memory composites are developed, realizing the complete visualization and precise localization of the device in vivo. The novel 4D printed cardiac occlusion device provides an effective way to reduce the risk of complications and contributes to versatility. It is expected to be a next-generation multifunctional repair device for personalized treatment of CHDs.
研究领域:
生物医学工程
·
心血管病学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）