PLoS Pathogens
·
2024
·Vol. 20
·期 7
·e1012410
·
IF 5.5
Xiaojun Zhong,Fuwen Liu,Tianqi Liang,Ranran Lu,Mengting Shi,Xiujuan Zhou,Menghua Yang
One of the greatest challenges encountered by enteric pathogens is responding to rapid changes of nutrient availability in host. However, the mechanisms by which pathogens sense gastrointestinal signals and exploit available host nutrients for proliferation remain largely unknown. Here, we identified a two-component system in Vibrio parahaemolyticus , TtrRS, which senses environmental tetrathionate and subsequently activates the transcription of the ttrRS - ttrBCA-tsdBA gene cluster to promote V. parahaemolyticus colonization of adult mice. We demonstrated that TsdBA confers the ability of thiosulfate oxidation to produce tetrathionate which is sensed by TtrRS. TtrRS autoregulates and directly activates the transcription of the ttrBCA and tsdBA gene clusters. Activated TtrBCA promotes bacterial growth under micro-aerobic conditions by inducing the reduction of both tetrathionate and thiosulfate. TtrBCA and TsdBA activation by TtrRS is important for V. parahaemolyticus to colonize adult mice. Therefore, TtrRS and their target genes constitute a tetrathionate-responsive genetic circuit to exploit the host available sulfur compounds, which further contributes to the intestinal colonization of V. parahaemolyticus .
Access to host nutrients is regarded as a critical step for the pathogen infection. However, the human gastrointestinal tract is colonized by an enormous number of microbes, which limits pathogens’ access to nutrient supplies. Sulfur is one of the most abundant elements on Earth and is also essential to all living organisms. The mechanisms by which bacteria, especially intestinal pathogens, regulate the bioavailability of utilizable sulfur in the host are poorly understood. Here, we characterized a tetrathionate-responsive genetic circuit, which was involved in the utilization of sulfur compounds including tetrathionate and thiosulfate in V. parahaemolyticus , and thus contributed to the bacterial intestinal colonization. Our study provides new insight into the ability of bacterial pathogens to sense gastrointestinal signals and exploit host nutrients for their colonization, which contributes to a better understanding of the pathogenesis of enteric pathogens.
研究领域:
微生物学
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病原生物学
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分子生物学
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DOI
PubMed
高影响因子
Food Bioscience
·
2020
·
IF 3.067
Guozhong Zhao,Man Cui,Mengying Wang,Wen Chen,Jingjing Li,Yunping Yao
Lactobacillus strains are used for their probiotic effects and fermentation properties. The colonization of 16 Lactobacillus strains was determined based on animal experimentation and Real-Time Quantitative PCR. Strains expressed varied amounts of colonization in the mouse gut environment, the best was shown by L. plantarum CCFM737 and L. casei CCFM736, followed by other L. plantarum and L. casei strains. L. plantarum CCFM737 and L. casei CCFM736 both colonized at a significant level for at least 2 wk. L. plantarum and L. casei showed the best biological properties, including gastrointestinal tolerance, antimicrobial activity , use of oligosaccharides , tolerance of gastrointestinal juices and adhesive properties, especially the antimicrobial properties. A significant correlation between colonization of L. plantarum and L. casei and biological properties was observed.
研究领域:
微生物学
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肠胃病学
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分子生物学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yulong Zhang,Suo Zheng,Jingru Chen,Zhiliang Cheng,Fei Liu,Yuehua Jiao
Lactococcus lactis undergoing aerobic respiration can consume oxygen and form an anaerobic environment. Salmonella , as a facultative anaerobic foodborne pathogen, multiply better under aerobic conditions. The purpose of this study was to explore aerobic respiration of Lactococcus lactis subsp. lactis ( L. lactis ) KLDS 4.0325 inhibitory effect on Salmonella enterica subsp. enterica serovar Typhimurium ( S. Typhimurium) SL 1344 in vitro and its colonization ability in vivo . Results indicate that L. lactis KLDS 4.0325 could inhibit the growth of S. Typhimurium SL1344 by producing bacteriocin. And L. lactis KLDS 4.0325 undergoing aerobic respiration could enhance this inhibitory effect, while reducing the expression levels of virulence genes sipB , sipC , and sopE2 in S. Typhimurium SL1344. L. lactis KLDS 4.0325 could be successfully labeled by CFDA-SE fluorescent probe, and the labeling rate was more than 97%. Murine experiments showed that L. lactis KLDS 4.0325 could adhere and colonize in the cecum and colon. Thus, L. lactis KLDS 4.0325 is a promising probiotic strain for the potential inhibition of Salmonella infection via its oxygen-scavenging in host gut.
研究领域:
微生物学
·
食品安全
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分子生物学
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产品关联
DOI
高影响因子
ISME Journal
·
2024
·
IF 10.8
Zheng Cao,Liu Dingqi,Lu Xinyu,Wu Huijun,Hua Jingyi,Zhang Chuang,Liu Kang,Li Changchun,He Jin,Du Cuiying
The ability of bacteria to use natural carbon sources greatly affects their growth and survival in the environment. Bacteria have evolved versatile abilities to use environmental carbon sources, but their diversity and assimilation pathways remain largely unexplored. Trans-aconitic acid, a geometric isomer of cis-aconitic acid involved in the tricarboxylic acid cycle, has long been considered a natural carbon source metabolizable by bacteria. However, its catabolism and ecological role in linking bacterial interactions with the environments remain unclear. Here, we identify a regulatory system in Bacillus velezensis FZB42 that is capable of sensing and catabolizing trans-aconitic acid. The system consists of a tar operon, an adjacent positive regulatory gene tarR, and a shared promoter. After receiving the trans-aconitic acid signal, the TarR protein interacts directly with the promoter, initiating the expression of the membrane transporter TarB and aconitate isomerase TarA encoded by the operon, which function in importing the trans-aconitic acid and isomerizing it into the central intermediate cis-aconitic acid. Subsequent soil colonization experiments reveal that trans-aconitic acid assimilating ability can give its coding bacteria a growth and competitive advantage. Bioinformatics analyses coupled with bacterial isolation experiments further show that the assimilation system of trans-aconitic acid is widely distributed in the bacterial domain, and its assimilating bacteria also extensively distributed in nature, indicating an important role of trans-aconitic acid metabolism in bacterial carbon acquisition. This work emphasizes the importance of metabolic adaptation to environmental carbon sources for bacterial survival and may provide inspiration for engineering microbes with enhanced environmental competitiveness.
研究领域:
微生物学
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环境科学
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分子生物学
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生态学
✓ 已验证
DOI
PubMed
高影响因子
BMC GASTROENTEROLOGY
·
2016
·Vol. 16
·期 1
·1-12
·
IF 2.101
Rutayisire Erigene,Huang Kun,Liu Yehao,Tao Fangbiao
Background The human gut is the habitat for diverse and dynamic microbial ecosystem. The human microbiota plays a critical role in functions that sustain health and is a positive asset in host defenses. Establishment of the human intestinal microbiota during infancy may be influenced by multiple factors including delivery mode. Present review compiles existing evidences on the effect of delivery mode on the diversity and colonization pattern of infants gut microbiota. Methods Two investigators searched for relevant scientific publications from four databases (Pubmed, Medline, Embase, and Web of Science). The last search was performed on September 21, 2015, using key terms ((delivery mode OR caesarean delivery OR cesarean section OR vaginal delivery) AND (gut microbiota OR gut microbiome OR gut microflora OR intestinal microflora OR microbial diversity) AND (infants OR children)). All included studies described at least two types of gut microbiota in relation to delivery mode (caesarean section vs vaginal delivery) and used fecal samples to detect gut microbiota. Results Seven out of 652 retrieved studies met inclusion criteria, were included in systematic analysis. Caesarean Section (CS) was associated with both lower abundance and diversity of the phyala Actinobacteria and Bacteroidetes, and higher abundance and diversity of the phylum Firmicute from birth to 3 months of life. At the colonization level, Bifidobacterium , and Bacteroides genera seems to be significantly more frequent in vaginally delivered infants compared with CS delivered. These infants were more colonized by the Clostridium , and Lactobacillus genera. From the reports, it is tempting to say that delivery mode has less effect on colonization and diversity of Bifidobacteria , Bacteroides, Clostridium, and Lactobacillus genera from the age of 6 to 12 months of life. Conclusion The diversity and colonization pattern of the gut microbiota were significantly associated to the mode of delivery during the first three months of life, however the observed significant differences disappears after 6 months of infants life. The healthy gut microbiota is considered to promote development and maturation of the immune system while abnormal gut is considered as the major cause of severe gastrointestinal infections during the infancy. Further studies should investigate the diversity and colonization levels of infant gut microbiota in relation to the mode of delivery and its broad impact on infants’ health at each stage of life.
研究领域:
微生物学
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儿科
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免疫学
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分子生态学
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发育生物学
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转化医学
✓ 已验证
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Bo Qiu,Huijuan Jing,Shengyi Han,Shuobo Zhang,Furong Ba,Lvwan Xu,Siyuan Xie,Wang Gao,Yilun Huang,Hang Xiao,David Julian McClements,Björn Berglund,Lanjuan Li,Mingfei Yao
Faecalibacterium prausnitzii ( F. prausnitzii ) exhibits a variety of biological functions that make it suitable for use as a next-generation probiotic . However, its high sensitivity to oxygen and digestive fluids currently limits its application. Riboflavin is known to support the growth of F. prausnitzii in oxygen environments, but it is important that it is in close proximity to the probiotics. Layer-by-layer assembly can be used to form protective coatings around probiotics, which can protect them from adverse environmental conditions. Moreover, riboflavin can be conjugated to these coatings, thereby increasing its efficacy by bringing it close to probiotic surfaces. In this study, we therefore evaluated the potential of electrostatic layer-by-layer assembly to protect F. prausnitzii by coating them with riboflavin-alginate and glycol-chitosan layers. Initially, we showed that riboflavin could be successfully conjugated to alginate, with a grafting ratio of around 4.35%. Then, the layer-by-layer method was used to coat F. prausnitzii using cationic glycol chitosan and anionic riboflavin-alginate. The coating formed was found to have a thickness of approximately 18.5 nm. Encapsulation did not adversely affect the growth of F. prausnitzii , but it significantly enhanced its resistance to oxygen and digestive fluids. The encapsulated probiotic was shown to have enhanced mucoadhesive properties using an in vitro intestinal monolayer model. Furthermore, the encapsulated probiotics colonized the colons of rats for longer than nonencapsulated ones. These results show that coating F. prausnitzii with riboflavin-rich biopolymer layers improves its resistance to oxygen and digestive fluids, and enhances its mucoadhesion and colonization properties, which should enhance its potential as an orally administered probiotic.
研究领域:
微生物学
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药物递送
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功能性食品
✓ 已验证
产品关联
DOI
高影响因子
CELL
·
2024
·
IF 45.5
Yuqi Wang,Mangze Hu,Jian Cao,Fengxiang Wang,Jingrong Regina Han,Tianshu William Wu,Luxiao Li,Jinshi Yu,Yujing Fan,Guanglei Xie,Heyuan Lian,Yueying Cao,Nathchar Naowarojna,Xi Wang,Yilong Zou
Metastatic dissemination to distant organs demands that cancer cells possess high morphological and metabolic adaptability. However, contributions of the cellular lipidome to metastasis remain elusive. Here, we uncover a correlation between metastasis potential and ferroptosis susceptibility in multiple cancers. Metastases-derived cancer cells exhibited higher ferroptosis sensitivity and polyunsaturated fatty acyl (PUFA)-lipid contents than primary-tumor-derived cells from ovarian cancer patients. Metabolism-focused CRISPR screens in a mouse model for ovarian cancer distant metastasis established via two rounds of in vivo selection revealed the PUFA-lipid biosynthesis enzyme acyl-coenzyme A (CoA) synthetase long-chain family member 4 (ACSL4) as a pro-hematogenous metastasis factor. ACSL4 promotes metastatic extravasation by enhancing membrane fluidity and cellular invasiveness. While promoting metastasis, the high PUFA-lipid state creates dependencies on abhydrolase-domain-containing 6, acylglycerol lipase (ABHD6), enoyl-CoA delta isomerase 1 (ECI1), and enoyl-CoA hydratase 1 (ECH1)—rate-limiting enzymes preparing unsaturated fatty acids (UFAs) for β-oxidation. ACSL4/ECH1 co-inhibition achieved potent suppression of metastasis. Our work establishes the dual functions of PUFA-lipids in tumor progression and metastasis that may be exploitable for therapeutic development.
研究领域:
癌症生物学
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代谢生物学
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脂质代谢
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分子肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Jiru Miao,Haiyang Chai,Longxing Niu,Mi Ouyang,Rong Wang
Catheter-associated urinary tract infection is one of the most common hospital-acquired infections. Proteus mirabilis, a common pathogen associated with urinary tract infections, has swarming motility and has pili on its surface for adhesion and flagella for upward movement. Migration of P. mirabilis along the catheter surface can lead to ascending urinary tract infection. However, there is currently a lack of effective strategies to inhibit or delay the colonization, migration, and encrustation formation of P. mirabilis in urinary catheters. This study developed a method for constructing a stable superhydrophobic coating on the surface of urinary catheters using a layer-by-layer approach. The adhesion and deposition of polydopamine were enhanced by pre-coating a liquid bandage film on the polydimethylsiloxane surface, resulting in a multilayer micro-nano composite structure on the catheter surface. This structure was combined with copper ions and superhydrophobic modifiers, ultimately achieving a highly stable superhydrophobic coating. The coating retains its superhydrophobic properties after prolonged incubation, friction tests, and tape peeling tests. Importantly, the coating demonstrates excellent efficacy in inhibiting P. mirabilis colonization, migration, and encrustation formation. This study offers novel insights into developing biomedical superhydrophobic coatings with enhanced stability and efficacy in inhibiting urinary tract bacterial infections.
研究领域:
生物医学工程
·
材料科学
·
微生物学
·
传染病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Translational Pediatrics
·
2021
·Vol. 10
·期 9
·2313
·
IF 2.488
Wei Shen,Wen Qiu,Yuting Liu,Weihua Liao,Yiyi Ma,Yan He,Zhang Wang,Hongwei Zhou
Background The gut microbiome plays a potential role in clinical events in preterm infants and may affect their lateral development. Understanding the initial colonization of microbes in the gut, their early dynamic changes, and the major factors correlated with these changes would provide crucial information about the developmental process in early life. Methods The present study enrolled 151 preterm infants and examined the longitudinal dynamics of their fecal microbiome profiles during the period of hospitalization using 16S ribosomal RNA gene sequencing. Random forest modeling was used to predict postnatal age (Age), postmenstrual age (PMA), and gestational age (GA), using gut microbiome features. Results Principal coordinate analysis revealed that the gut microbiome of the preterm infants displayed an obvious time-dependent change pattern, which showed the strongest association with Age, followed by PMA, and a much weaker association with (GA). Random forest modeling further evidenced the time-dependent change pattern, with the Pearson’s correlation coefficients between the actual values and the gut microbiome-predicted values being 0.68, 0.53, and 0.38 for postnatal, postmenstrual, and gestational age, respectively. The microbiome dynamism could be further divided into four Age stages, each with its own characteristic microbial taxa. The first 1–4 days (T1 stage) represented the meconium microbiome, with colonization of a high diversity of microbes before or during delivery. During 5–15 days (T2 stage), the gut microbiome of the preterm infants underwent a rapid turnover, in which microbial diversity declined, and stabilized afterward. Enterobacteriaceae , Enterococcaceae , Streptococcaceae , Staphylococcaceae , and Clostridiaceae were the major classes in the gut microbiome in the lateral stages of development (T3–T4 stage). Conclusions Postnatal age, rather than the gestational age, is significantly correlated with the gut microbiome of preterm infants, suggesting that clinical interventions contribute more to the early dynamics of gut microbiome in preterm infants than the natural development of the gut.
研究领域:
微生物组学
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新生儿科学
·
发育生物学
·
临床微生物学
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生物信息学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 7
·11214-11227
·
IF 0
Li Tiantian,Yang Huan,Zhang Naili,Dong Lijia,Wu Aiping,Wu Qiqian,Zhao Mingshui,Liu Hua,Li Yan,Wang Yanhong
Cadmium (Cd) contamination is a widespread environmental issue. There is a lack of knowledge about the impacts of applying arbuscular mycorrhizal fungi (AMF) and biochar, either alone or in their combination, on alleviating Cd phytotoxicity in Ligustrum lucidum . Therefore, a pot experiment was conducted in a greenhouse, where L. lucidum seedlings were randomly subjected to four regimes of AMF treatments (inoculation with sterilized AMF, with Rhizophagus irregularis , Diversispora versiformis , alone or a mixture of these two fungi), and two regimes of biochar treatments (with or without rice-husk biochar), as well as three regimes of Cd treatments (0, 15, and 150 mg kg −1 ), to examine the responses of growth, photosynthetic capabilities, soil enzymatic activities, nutritional concentrations, and Cd absorption of L. lucidum plants to the interactive effects of AMF, biochar, and Cd. The results demonstrated that under Cd contaminations, AMF alone significantly increased plant total dry weight, soil pH, and plant nitrogen (N) concentration by 84%, 3.2%, and 13.2%, respectively, and inhibited soil Cd transferring to plant shoot by 42.2%; biochar alone significantly enhanced net photosynthetic rate, soil pH, and soil catalase of non-mycorrhizal plants by 16.4%, 9%, and 11.9%, respectively, and reduced the soil Cd transferring to plant shoot by 44.7%; the additive effect between AMF and biochar greatly enhanced plant total dry weight by 101.9%, and reduced the soil Cd transferring to plant shoot by 51.6%. Furthermore, dual inoculation with D. versiformis and R. irregularis conferred more benefits on plants than the single fungal species did. Accordingly, amending Cd-contaminated soil with the combination of mixed-fungi inoculation and biochar application performed the best than either AMF or biochar alone. These responses may have been attributed to higher mycorrhizal colonization, soil pH, biomass accumulation, and biomass allocation to the roots, as well as photosynthetic capabilities. In conclusion, the combined use of mixed-fungi involving D. versiformis and R. irregularis and biochar addition had significant synergistic effects on enhancing plant performance and reducing Cd uptake of L. lucidum plants in Cd-contaminated soil. Graphical abstract
研究领域:
环境科学
·
植物科学
·
土壤生物学
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微生物学
·
生态毒理学
✓ 已验证
DOI
MACROMOLECULAR BIOSCIENCE
·
2023
·
IF 4.6
Xiuxia Sun,Qi Chen,Angelique A. Guan,Shuaishuai Yuan,Zhibo Li
Abdominal wall defect caused by surgical trauma, congenital rupture, or tumor resection may result in hernia formation or even death. Tension-free abdominal wall defect repair by using patches is the gold standard to solve such problems. However, adhesions following patch implantation remain one of the most challenging issues in surgical practice. The development of new kinds of barriers is key to addressing peritoneal adhesions and repairing abdominal wall defects. It is already well recognized that ideal barrier materials need to have good resistance to nonspecific protein adsorption, cell adhesion, and bacterial colonization for preventing the initial development of adhesion. Herein, electrospun poly(4-hydroxybutyrate) (P4HB) membranes infused with perfluorocarbon oil are used as physical barriers. The oil-infused P4HB membranes can greatly prevent protein attachment and reduce blood cell adhesion in vitro. It is further shown that the perfluorocarbon oil-infused P4HB membranes can reduce bacterial colonization. The in vivo study reveals that perfluoro(decahydronaphthalene)-infused P4HB membranes can significantly prevent peritoneal adhesions in the classic abdominal wall defects’ model and accelerate defect repair, as evidenced by gross examination and histological evaluation. This work provides a safe fluorinated lubricant-impregnated P4HB physical barrier to inhibit the formation of postoperative peritoneal adhesions and efficiently repair soft-tissue defects.
研究领域:
生物医学工程
·
材料科学
·
外科实践
·
组织工程
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
iScience
·
2024
·
IF 4.6
Ming-Xin Cao,Zhan-Yin Qian,Yan-Jie Liang,Qi-Yao Liu,Han-Ping Wang,Yang Meng,Yin-Song Wang,Yue Wang
Dental caries is a common disease resulting from tooth demineralization caused by bacterial plaque. Probiotics have shown great potential against caries by regulating the balance of oral flora. However, obstacles such as poor colonization and lysozyme sensitivity in oral cavity hinder their further application. In this study, an efficient layer-by-layer surface coating of tannic acid (TA)-Ca 2+ and casein phosphopeptide (CPP) was applied to the probiotic Bifidobacterium infantis (BI) with potential anti-caries activity. Multi-functionalized probiotics thus prepared (called BI@TA-Ca 2+ @CPP) exhibited similar growth pattern, resistance to lysozyme and enhanced colonization potential. The ability of BI@TA-Ca 2+ @CPP in inhibiting cariogenic biofilm formation was improved. Moreover, BI@TA-Ca 2+ @CPP showed excellent remineralization efficacy. In a caries-induced rat model, BI@TA-Ca 2+ @CPP significantly prevented the occurrence and progression of tooth decay, meanwhile showing good biocompatibility. In summary, this study underlines the importance of probiotics coating in antibiofilm and remineralization activity as a promising strategy against dental caries.
研究领域:
龋齿预防
·
口腔微生物学
·
益生菌工程
·
生物矿化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）