Journal of Biophotonics
·
2018
·Vol. 12
·期 2
·e201800134
·
IF 3.768
Jianyi Xu,Yilin Ma,Tingting Yu,Dan Zhu
Various tissue optical clearing techniques have sprung up for large volume imaging. However, there are few methods showed clearing and imaging data on different organs while most of them were focused on mouse brain, and as a result, it is difficult to select the suitable method for organs in practical applications due to lack of quantitative evaluation and comprehensive comparison. Therefore, it is necessary to evaluate and compare the performances of clearing methods for different organs. In this paper, several typical optical clearing methods were applied, including 3DISCO, uDISCO, SeeDB, FRUIT, CUBIC, Sca l eS and PACT to clear intact brain, heart, kidney, liver, spleen, stomach, lung, small intestine, skin and muscle. The clearing efficiency, sample deformation, fluorescence preservation and imaging depth of these methods were quantitatively evaluated. Finally, based on the systemic evaluation of various parameters described above, the appropriate clearing method for specific organ including kidney or intestine was screened out. This paper will provide important references for selection of appropriate clearing methods in related researches.
研究领域:
生物医学工程
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显微成像
·
组织学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Ligui Wu,Xiaoyu Xiao,Fen Chen,Huan Zhang,Liangliang Huang,Lingling Rong,Xiaoming Zou
Antibiotic resistance genes (ARGs) have been widely detected around the world and are generally viewed as emerging pollutants with environmental persistence. The proliferation of ARGs can be easily promoted by antibiotics. However, the dynamics of ARGs in the environment are still unable to be quantified using a single parameter, which is vital to evaluating the ability of ARGs to spread by antibiotics and effectively controlling ARGs. A new parameter, termed the relative area ratio of sample to control (Δ A R ), was developed based on the quantitative features determined by ARG-time curves in soils contaminated with sulfonamides (SAs) and verified by quantitative structure-activity relationships (QSARs) models. The results showed that Δ A R can not only be used to accurately quantify the characteristics of SAs resistance genes ( Suls ) over time but also be applied to reveal the relationships between the proliferation of Suls and important factors (i.e., concentrations and chemical structures). Moreover, the Δ A R -based QSARs model indicated that bioavailability and the frequency of conjugative transfer, rather than the ability of induced mutations in bacteria, tend to be key processes of the characteristics of the proliferation of Suls . Therefore, Δ A R is a useful parameter to perform environmental risk assessments of ARG proliferation in the environment.
研究领域:
环境科学
·
微生物学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Nan Zhao,Feng Ju,Quanwei Song,Zhiwen Qi,Hao Ling
Soil organic matter (SOM) is considered as a pivotal factor influencing the adsorption of pollutants. However, few prior quantitative investigations of the SOM functional group distribution to the contaminants' fate have been conducted. In this paper, the SOM cluster method based on COSMO-RS theory has been conducted to illustrate the chemical composition variables of SOM that affect the polycyclic aromatic hydrocarbons (PAHs) fate in quantitative terms. In the theoretical simulations, the contributions of carbonyl, carboxyl, aromatic, oxyalkyl and aliphatic groups in SOM to phenanthrene (Phe) and pyrene (Pyr) adsorption are evaluated by calculating the partition coefficients ( LogP ). The results show that the increase in oxyalkyl content leads to a decrease in LogP . Inversely, carbonyl and carboxyl groups of SOMs positively associated with Phe adsorption. The changes in aromatic and alkyl components have a similar magnitude of influence on LogP . Moreover, the effect of non-carbon-based functional groups in SOM on the Phe partitioning has been examined for the first time. The increase of sulfur and nitrogen content in SOM hinder Phe adsorption, while the rise of phosphorus content promotes the adsorption. In soil adsorption experiments, four natural soils, characterized by X-ray photoelectron spectroscopy (XPS) and Diffuse reflectance infrared Fourier transform (DRIFT), are selected to verify the influence of SOM functional group distribution. Comparing the experimental SOM-water partition coefficient ( LogK oc ) with the simulation predicted LogP suggests that the COSMO-RS based SOM cluster method can predict PAHs adsorption ability in SOM.
研究领域:
环境科学
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化学
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DOI
PubMed
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2025
·
IF 5
Yang Jiao,Jinyang Wang,Yingchao Zhang,Yinji Ma,Xue Feng
Bioelectronics bridges the gap between electronic devices and biological systems through interfaces. The conformability of interfaces is significant in improving the quality of acquired physiological signals. However, practical methods for conformal assessment of arbitrary interfaces are still lacking. Mechanical predictions are imprecise for soft tissue that has curved shapes and is under dynamic deformation. Thus, current conformal assessment relies on visual inspections of contact status or feedback derived from acquired signals. Here, a quantitative and non-contact method is proposed to assess the conformability of bioelectronics on tissue. Biocompatible speckles enhance feature points on the target surface, and its deformed shape is reconstructed based on binocular stereo vision. A normalized metric, mean gap height, is proposed for conformal assessment, which intuitively quantifies conformability as a percentage and reflects the gap caused by partial contact. The method is applied to assess the conformability of a stretchable film covering a static hemisphere, and the results highlight the effects of various conformal strategies and offer guidelines to prevent overdesign. Furthermore, the method illustrates the interface evolution of surface electromyography devices during a muscle contraction cycle, demonstrating potential applications in optimizing electrophysiological monitoring interfaces for daily healthcare.
研究领域:
生物医学工程
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柔性电子器件
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生理信号采集
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机械变形分析
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产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Mingyu Zhao,Fei Jiang,Zheng Li,Yeqiang Lu,Guojun Jiang,Zhijuan Sun,Lixin Xue
Super-hydrophobic none woven fabrics (NWFs) made of polypropylene (PP) based fibers and porous fibers with the cross-sectional shapes of round (R p ), tri-lobal (Y p ) and C p shape were prepared and combined with oxygen sensing probes to construct on-line oil spillage monitoring systems. The effects of fiber cross sectional shapes and surface micro-porosity on qualitative response time (RT), signal/noise ratio (SNR) were systematically investigated, for the first time, together with spilled oil viscosity and surfactant content in water. Reliable low detection limit (RLDL) was found decreased from 0.28 to 0.20, 0.12 and 0.10 g/L, respectively, when the fibers were changed from conventional R shape to C shape, C p shape and F-SiO 2 @C p shape. Quantitative determination of oil content in emulsions or floating oil layers were found to be feasible based on the detected linear relationship between ln values of oil content with obtained response time (RT), or between the oil content with obtained signal-noise ration (SNR) values. In time (10–60 s) qualitative responses to early stage very small (spilled area close to 5 cm 2 ) oil spillages and onsite quantitative assessment of ultra-low oil contaminating levels (0.1 g/L) can be realized in a single detection probe for the first time.
研究领域:
材料科学
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环境监测
·
化学传感
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DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 9
·1-9
·
IF 6.232
Yin Zhenzhen,Cheng Xiaoli,Wang Gan,Chen Jia,Jin Yan,Tu Qiuyun,Xiang Juan
A highly sensitive and specific surface plasmon resonance (SPR) method using one anti-alpha-synuclein antibody (anti-αS) and titanium phosphate nanoparticles (Ti 4+ @TiP) was developed for quantitative evaluation of phosphorylated αS level which was defined by the ratio of p-αS to total alpha-synuclein (t-αS) (p-αS/t-αS). The close affinities of anti-αS to αS (0.975 pM −1 ) and p-αS (0.938 pM −1 ) were obtained. Based on this fact , both αS forms were simultaneously captured and the t-αS was quantified using the anti-αS immobilized Au chip. With the selective recognition of Ti 4+ @TiP nanoparticles, the p-αS was quantified. The dynamic ranges of our method were 1.0~20.0 pg mL −1 for the detection of t-αS and 0.1~10.0 pg mL −1 for that of p-αS. The analysis of αS- and p-αS-spiked artificial cerebrospinal fluid samples revealed the high accuracy of the method. Furthermore, the concentrations of αS and p-αS in clinical CSF samples collected from three healthy donors were determined and displayed a high correlation with the results from a commercial ELISA kit, confirming the viability and of the proposed method. The method is convenient, economical, and practical for the evaluation of phosphorylated αS level with high sensitivity and selectivity. It is of great significance for the early diagnosis of PD and the evaluation of PD progression. Graphical abstract
研究领域:
生物传感器
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纳米技术
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神经退行性疾病
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分析化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Zhao Sheng Qian,Lu Jing Chai,Yuan Yuan Huang,Cong Tang,Jia Jia Shen,Jian Rong Chen,Hui Feng
A convenient and real-time fluorometric assay with the assistance of copper ions based on aggregation and disaggregation of carbon quantum dots (CQDs) was developed to achieve highly sensitive detection of alkaline phosphatase activity. CQDs and pyrophosphate anions (PPi) were used as the fluorescent indicator and substrate for ALP activity assessment respectively. Richness of carboxyl groups on the surface of CQDs enables their severe aggregation triggered by copper ions , which results in effective fluorescence quenching . Under the catalytic hydrolysis of ALP, PPi can be rapidly transformed to phosphate ions. Stronger affinity of phosphate ions to copper ions than carboxyl groups is taken advantage of to achieve fluorescence recovery induced by re-dispersion of CQDs in the presence of ALP and PPi. Quantitative evaluation of ALP activity in a broad range from 16.7 to 782.6 U/L with the detection limit of 1.1 U/L can be realized in this way, which endows the assay with high enough sensitivity for practical detection in human serum. This strategy broadens the sensing application of fluorescent CQDs with excellent biocompatibility, and provides an example based on disaggregation in optical probe development.
研究领域:
分析化学
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生物化学
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生物医学工程
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Yao Tian,Zhipeng Zhang,Nan Gao,Pengcheng Huang,Fang–Ying Wu
In this work, a label-free, selective, and sensitive luminescent sensing platform was established for tyrosinase (TYR) activity monitoring and its inhibitor screening using one kind of lanthanide coordination polymer nanoparticles AMP–Tb/Ag + . By taking advantage of the specific binding and redox properties of Ag + incorporated into the AMP–Tb network and dopamine (DA) as the product of the model substrate tyramine , the enzymatic reaction and the signal change of the sensing platform was effectively linked. The cooperative effect of a weakened energy transfer from AMP to Tb 3+ by altering the electronic structure of Ag + and an efficient photoinduced election transfer (PET) process caused by dopaquinone facilitated the luminescence quenching of Tb 3+ . Thus, this luminescent sensing platform could be employed for quantitative evaluation of TYR activity. There was a good linear range for TYR activity from 0.08 to 0.20 U mL − 1 with a low detection limit of 0.004 U mL − 1 . Furthermore, this assay was successfully applied to accurate determination of TYR activity in human serum samples and efficient screening of TYR inhibitors. Considering unique spectral characteristics of lanthanides along with operation simplicity and superior analytical performance, this sensing platform is very promising in clinical diagnosis and drugs screening for TYR–associated diseases.
研究领域:
分析化学
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生物医学工程
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材料科学
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生物化学
·
临床诊断
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Zhixin Wang,Yuanpeng Fu,Tianzhao Bu,Yuqi Qiu,Bo Pang,Qingyang Zheng,Sen Zhou,Yangyang Li,Xiling Xiao,Zhouping Yin,Hao Wu
Quantitative and objective assessment of muscle spasticity grades in post-stroke patients plays a pivotal role for physicians and patients in adjusting rehabilitation plans and preventing secondary complications. However, the development of such methods for spasticity detection and assessment is hindered by the subjectivity of conventional assessment methods, the limited clinical applicability of digital-devices, and the structural complexity of sensors used for monitoring muscle strength. Here, a multimodal on-skin sensor is developed, enabling simultaneous acquisition of surface electromyography (sEMG) and triboelectric signals, through wavelet analysis, the signal from the sensor can comprehensively reflect the bioelectrical and biomechanical characteristics of human motion. The high-performance triboelectric materials (BPA NPs) with metal-organic–inorganic core-shell structures are synthesized to enhance the signal quality of the multimodal on-skin sensor by leveraging the self-polymerization ability and weak reducibility of dopamine. The sensitivity of the multimodal on-skin sensor is improved by designing a radially arranged micro-cone array with gradient heights. Additionally, a quantitative spasticity assessment strategy is developed by integrating muscle co-activation coefficients (Index 1) and antagonistic efficacy metrics (Index 2), which are strongly correlated with the Modified Ashworth Scale scores. The multimodal on-skin sensor with the proposed assessment strategy enables quantitative assessment of spasticity in 9 spasticity patients.
研究领域:
生物医学工程
·
可穿戴传感器
·
神经康复
·
信号处理
·
智能材料
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产品关联
DOI
高影响因子
Sustainability
·
2024
·Vol. 16
·期 19
·8568
·
IF 3.3
Yuan-Yuan Wang,Dan-Feng Zhang,Hong-Rui Zhang,Wen-Jun Liu,Zhi-Quan Chen,Jun-Cheng Jiang,Lei Ni
In this study, sodium dodecylbenzene sulfonate was used as a stabilizer, and NaOH, TBHP, and benzoyl chloride were used as reactants in the preparation of tert–butyl peroxybenzoate (TBPB) using a two–step process. The process conditions were optimized by a three–factor, three–level Box–Behnken design approach. The results showed that the yield of TBPB achieved 88.93% under the optimum conditions of temperature of 31.50 °C, feeding time of 22.00 min, and NaOH concentration of 15%. The exothermic properties of the synthesis of TBPB were investigated using reaction calorimetry. The thermal decomposition characteristics of reactants and products were analyzed by differential scanning calorimetry (DSC) and accelerating rate calorimetry (ARC), and the changes in substance types, characteristic peaks, and exothermic quantities during the reaction were analyzed before and after the reaction by FTIR. The reaction mechanism was proposed by combining EasyMax 102, RC1e, gas chromatography (GC), and Fourier transform infrared spectrometry (FTIR). A comprehensive study of the reaction mechanism and reaction exotherm was carried out using density functional theory (DFT) to predict the reaction energy change and the direction of the reaction and to determine whether the reaction was reversible or not. The risk level for the synthesis of TBPB in semi–batch mode was evaluated using a risk matrix and the Stoessel criticality diagram. The optimal conditions for the TBPB synthesis process in a plate microreactor were explored. Both microreactors and semi–batch modes were comparatively analyzed using the m–ITHI quantitative assessment method. The results indicated a hazard class 2 in semi–batch mode and a hazard class 1 in the microreactor. The results of the study may provide a reference for the further improvement of the intrinsically safe design of the synthetic TBPB process.
研究领域:
化学工程
·
过程安全
·
热分析
·
反应工程
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DOI
高影响因子
危险品化学品经营许可证（带存储）