Toxins
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2020
·Vol. 12
·期 8
·521
·
IF 3.531
Xia Hong,Yuhao Mao,Chuqin Yang,Zhenjiang Liu,Ming Li,Daolin Du
Zearalenone (ZEN) is a prevalent mycotoxin that needs intensive monitoring. A semi-quantitative and quantitative immunochromatographic assay (ICA) was assembled for investigating ZEN contamination in 187 samples of cereal and their products from China in 2019. The semi-quantitative detection model had a limit of detection (LOD) of 0.50 ng/mL with visual judgment and could be completely inhibited within 5 min at 3.0 ng/mL ZEN. The quantitative detection model had a lower LOD of 0.25 ng/mL, and ZEN could be accurately and digitally detected from 0.25–4.0 ng/mL. The ICA method had a high sensitivity, specificity, and accuracy for on-site ZEN detection. For investigation of the authentic samples, the ZEN-positive rate was 62.6%, and the ZEN-positive levels ranged from 2.7 to 867.0 ng/g, with an average ZEN-positive level being 85.0 ng/g. Of the ZEN-positive samples, 6.0% exceeded the values of the limit levels. The ZEN-positive samples were confirmed to be highly correlated using LC-MS/MS (R2= 0.9794). This study could provide an efficiency and accuracy approach for ZEN in order to achieve visual and digitized on-site investigation. This significant information about the ZEN contamination levels might contribute to monitoring mycotoxin occurrence and for ensuring food safety.Keywords:zearalenone;immunochromatographic assay;semi-quantification;quantificationKey Contribution:A semi-quantitative and quantitative test strip had been developed and applied for investigating zearalenone contamination in 187 authentic samples in China in 2019.
研究领域:
食品安全
·
分析化学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Xiu-Fang Yan,Zeng-Ping Chen,Ying Huang,Chao Kang,Ru-Qin Yu
Probe encapsulated by biologically localized embedding (PEBBLE) has emerged as a new type of sensing technique for complex systems. Generalized ratiometric PEBBLE nanosensors prepared by encapsulating an intensity-based probe and an inert reference dye inside the pores of stable matrix possess advantages of easy synthesis, immunity to interference, lower toxicity, and robustness to variations in probe loading. However, the selection of appropriate reference dyes used in generalized ratiometric PEBBLE nanosensors is a rather difficult task since they should satisfy some stringent requirements. In this contribution, the feasibility of using carbon dots (C-dots) as generic inert references in synthesizing PEBBLE nanosensors was first investigated in detail. And a dual-wavelength monitoring strategy and the quantitative fluorescence model for generalized ratiometric probes (QFM GRP ) were adopted to solve the problems brought by the use of carbon dots as inert references. C-dots doped PEBBLE nanosensors (C-PEBBLE nanosensors) for the quantification of NO 2 − and free Ca 2+ were synthesized by encapsulating C-dots and intensity based fluorescence probes (i.e., acriflavine for NO 2 − , and Rhod-2 for Ca 2+ , respectively) inside the pores of stable matrix. Experimental results showed that the combination of C-PEBBLEs, the QFM GRP model and the dual-wavelength monitoring strategy achieved accurate quantification of NO 2 − and the free Ca 2+ in real-world samples. Their quantitative results were in good consistence with those determined by HPLC and atomic absorption spectrophotometer, respectively. The strategies proposed in this contribution have generic applicability in the synthesis of PEBBLE nanosensors and their quantitative applications.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2020
·Vol. 101
·期 4
·1659-1665
·
IF 2.614
Yuhao Mao,Mingna Sun,Xia Hong,Sagnik Chakraborty,Jinsheng Duan,Ming Li,Daolin Du
BACKGROUND The toxicity and health risks of mycotoxins have encouraged increased awareness and strict monitoring of these contaminants in agricultural by-products. In this paper, we developed and tested a sensitive, selective, and self-assembling immunochromatographic (IC) strip for on-site detection of ochratoxin A (OTA). We were able to demonstrate semi-quantitative and quantitative detection of OTA in agricultural by-product samples. RESULTS The optimized IC strip had a limit of detection (LOD) of 0.5 ng mL −1 OTA using the naked eye for semi-quantitative detection. When a digitized strip reader was used to achieve quantitative results, the LOD for OTA was reduced to 0.1 ng mL −1 with a linear detection range of 0.1–10 ng mL −1 . We also evaluated the specificity, stability, accuracy, and precision of the IC strip and demonstrated high performance in all of these areas. We then confirmed the ability of the IC strip to visually detect OTA contamination in authentic agricultural by-product samples from the markets in China. Furthermore, quantitative detection of OTA using the IC strip showed that the concentration of OTA ranged from 1.7 to 101.3 ng g −1 in the positive agricultural by-product samples, correlating well with the measurements obtained via liquid chromatography with tandem mass spectrometry. CONCLUSION The results indicated that this proposed IC strip was capable of sensitive, semi-quantitative, quantitative, and on-site detection of OTA contamination in agricultural by-product samples. © 2020 Society of Chemical Industry
研究领域:
食品安全
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分析化学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Yueyi Zhang,Haihui Ren,Xiao Tang,Qiaorong Liu,Wen Xiao,Zunjian Zhang,Yuan Tian
The formidable challenge posed by the presence of extremely high amounts of compounds and large differences in concentrations in plasma significantly complicates non-targeted metabolomics analyses. In this study, a comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry (GC×GC-qMS) method with a solid-state modulator (SSM) for non-targeted metabolomics in beagle plasma was first established based on a GC-MS method, and the qualitative and quantitative performance of the two platforms were compared. Identification of detected compounds was accomplished utilizing NIST database match scores, retention indices (RIs) and standards. Semi-quantification involved the calculation of peak area ratios to internal standards. Metabolite identification sheets were generated for plasma samples on both analytical platforms, featuring 22 representative metabolites chosen for validating qualitative accuracy, and for conducting comparisons of linearity, accuracy, precision, and sensitivity. The outcomes revealed a threefold increase in the number of identifiable metabolites on the GC×GC-MS platform, with lower limits of quantitation (LLOQs) reduced to 0.5–0.05 times those achieved on the GC-MS platform. Accuracy in quantification for both GC×GC-MS and GC-MS fell within the range of 85–115%, and the vast majority of intra- and inter-day precisions were within the range of 20%. These findings underscore that relative to the conventional GC-MS method, the GC×GC-MS method developed in this study, combined with SSM, exhibits enhanced qualitative capabilities, heightened sensitivity, and comparable accuracy and precision, rendering it more suitable for non-targeted metabolomics analyses.
研究领域:
分析化学
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代谢组学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
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2015
·
IF 3.545
Yakun Lian,Hua Jiang,Jinzhou Feng,Xiaoyan Wang,Xiandeng Hou,Pengchi Deng
ATP , ADP and AMP are energy substances with vital biological significance. Based on the structural differences, a simple, rapid and comprehensive method has been established by 1 H and 31 P Nuclear Magnetic Resonance ( 1 H-NMR and 31 P-NMR) spectroscopies. Sodium 3-(trimethylsilyl) propionate-2,2,3,3-d 4 (TMSP) and anhydrous disodium hydrogen phosphate (Na 2 HPO 4 ) were selected as internal standards for 1 H-NMR and 31 P-NMR, respectively. Those three compounds and corresponding internal standards can be easily distinguished both by 1 H-NMR and 31 P-NMR. In addition, they all have perfect linearity in a certain range: 0.1–100 mM for 1 H-NMR and 1–75 mM for 31 P-NMR. To validate the precision of this method, mixed samples of different concentrations were measured. Recovery experiments were conducted in serum (91–113% by 1 H-NMR and 89–113% by 31 P-NMR).
研究领域:
生物化学
·
医学检测
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 3
·1036-1045
·
IF 1.938
Xiaozhi Wang,Weijun Zhao,Lingfeng Li,Xiangyang Wang,Peng Li,Yu Wang,Jikui Luo
Sulfites (sulfur dioxide) and inorganic sulfites are types of food additives and preservatives, widely used in the production of food and herbal medicines (HMs). However, the overingestion of sulfites and its associates are harmful to human health and may cause medical complications. Various methods and instruments have been developed for measuring sulfites in foods and HMs, but they typically suffer from many issues, such as high detection limitation, inaccurate and non-reliable results, time and labor-intensive sample preparation, and high cost. Herein, we present a fast, sensitive, and quantitative method to determine sulfites in HMs using field asymmetric-wave ion mobility spectrometry (FAIMS) coupled with a headspace bubbling method. The headspace air bubbling method is effective and efficient at generating stable sulfur dioxide (SO2) in the gas phase for FAIMS analysis. It allows sulfites with a concentration down to 1 mg kg−1 to be easily detected by this new method in 20 min, much shorter than with current technologies. The limits of detection (LOD) and limits of quantification (LOQ) are 1 mg kg−1 and 3 mg kg−1 in HMs, respectively. The new method is of great significance to ensure medical safety and for HM production quality control.
研究领域:
分析化学
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药学
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食品安全
✓ 已验证
产品关联
DOI
高影响因子
Separations
·
2022
·Vol. 9
·期 6
·140
·
IF 3.344
Xue Li,Wei Wang,Suling Sun,Junhong Wang,Jiahong Zhu,Feng Liang,Yu Zhang,Guixian Hu
A method for quantifying the anthocyanins in grapes was firstly developed by ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOFMS) combined with quantitative analysis of multi-components by single marker (QAMS). A total of 10 main anthocyanins were analyzed by using peonidin 3-O-glucoside as the reference standard. The accuracy of this method was evaluated by an established and validated external standard quantification method with 10 reference compounds. The standard method difference (SMDs) of the quantification results between QAMS and the external standard methodwasless than 15%. Furthermore, the QAMS method was used to analyzefour batches of grapes and the data was compared with those obtained using the external standard method. No significant difference wasobtained in the results obtained by both methods. These results indicated that the QAMS method could accurately determine the anthocyanins in grapes. This method can provide a basis to address the absence of reference standards for analyzing anthocyanins in other foods.
研究领域:
食品科学
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分析化学
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植物学
✓ 已验证
DOI
高影响因子
Food Quality and Safety
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2025
·
IF 4.4
Hu Jinyan,Liu Xiaogang,Liu Tiantian,Zhao Ting,Li Mingliang,Yang Qilin,Jiang Xiaofeng,Xiong Yanfei,Shen Caihong,Liu Shuangping,Mao Jian,Tu Rongkun
ObjectivesYeast plays a crucial role in fermented foods, yet the application of the CRISPR/Cas system for yeast detection has been rarely reported. The objective of this study is to develop a rapid and quantitative detection method for brewing yeast based on the CRISPR-Cas12a system, aiming to achieve higher sensitivity than the quantitative real-time polymerase chain reaction (qPCR) assay and fill the gap in the application of CRISPR for yeast detection in fermented foods.Materials and Methods: This work established one-step RPA-CRISPR/Cas12a (ORCC) detection for quantifying yeasts in fermented foods by integrating target amplification and the CRISPR system into a one-pot reaction.ResultsA numerical standardized definition was established for the quantifiable state in ORCC detection of yeasts. Using ORCC detection, which occurs at 37 °C, absolute quantification of Saccharomyces cerevisiae, Wickerhamomyces anomalus, and Zygosaccharomyces bailii can be completed in less than 45 min. Under optimal conditions, the limits of quantification and the limits of detection for three yeasts were 100 copies/μL and 1 copy/μL, with no cross-reaction observed among non-target yeasts. In pure yeast samples, ORCC detection of the target yeasts achieved a detection rate of 91.60% - 116.57%. In simulated fermentation samples, the recovery rate for spiked target yeasts using ORCC detection ranged from 92.78% to 106.90%. In original naturally fermented samples, target yeasts can be quantified by ORCC detection but not by qPCR assay. In spiked naturally fermented samples, the recovery rates of the qPCR assay were generally lower than those of ORCC detection.ConclusionORCC detection is simple, rapid, accurate, and highly sensitive for detecting three yeasts in fermented foods. It has significant potential for precisely monitoring yeast growth and fermentation process in various fermentation systems.
研究领域:
微生物学
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分子生物学
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食品科学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 5
·2022
·
IF 4.6
Ting Zhang,Dingrong Wan,Yuanyuan Li,Sisi Wang,Xiuteng Zhou,Fatemeh Sefidkon,Xinzhou Yang
TheArtemisia argyiLévl. et Van. growing in the surrounding areas of Qichun County in China are called Qiai (QA). Qiai is a crop that can be used both as food and in traditional folk medicine. However, detailed qualitative and quantitative analyses of its compounds remain scarce. The process of identifying chemical structures in complex natural products can be streamlined by combining UPLC-Q-TOF/MS data with the UNIFI information management platform and its embedded Traditional Medicine Library. For the first time, 68 compounds in QA were reported by the method in this study. The method of simultaneous quantification of 14 active components in QA using UPLC-TQ-MS/MS was reported for the first time. Following a screening of the activity of QA 70% methanol total extract and its three fractions (petroleum ether, ethyl acetate, and water), it was discovered that the ethyl acetate fraction enriched with flavonoids such as eupatilin and jaceosidin had the strongest anti-inflammatory activity, while the water fraction enriched with chlorogenic acid derivatives such as 3,5-di-O-caffeoylquinic acid had the strongest antioxidant and antibacterial activity. The results provided the theoretical basis for the use of QA in the food and pharmaceutical industries.Keywords:Artemisia argyiLévl. et Van.;UPLC-MS;quantification;anti-inflammatory;antioxidant
研究领域:
药理学
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分析化学
·
传统医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Lin Zhu,Xiaoyue Su,Hao Ma,Lianze Shan,Bing Zhao
Spectra in surface-enhanced Raman scattering (SERS) are always accompanied by a continuous emission known as “background”, which complicates the analysis with a poor signal-to-noise ratio (SNR), especially for qualitative and quantitative detection. Here, the SERS profile of the CH 3 NH 3 PbBr 3 substrate displays high similarity with the bulk Raman spectra, and its FWHM is exceeding narrow. The significantly increased SNR of the probe molecules indicates that the substrate helps to improve the quantitative analysis capability of the SERS technology and alleviate the background-related problems. For the CH 3 NH 3 PbBr 3 substrate, good linearity (R 2 = 0.9591) in the range of 1.0 × 10 -7 − 1.0 × 10 -3 mol/L was obtained and the limit of detection (LOD) is 7.88 × 10 -6 mol/L. The reason for this excellent performance is that the introduction of organic molecules splits the 3D CH 3 NH 3 PbBr 3 substrate to form a 2D perovskite that fixes the molecular orientation through chemical interactions. The CH 3 NH 3 PbBr 3 has the advantage of maintaining the conformation and orientation consistency of adsorbed molecules. Meanwhile, compared with the commonly used noble metal (Au, Ag) and semiconductor (ZnO, TiO 2 ) SERS substrates, the CH 3 NH 3 PbBr 3 substrate has obvious superiorities in both spectral peak intensity and peak shape. Furthermore, the CH 3 NH 3 PbBr 3 substrate possesses specific recognition ability that has the same enhancement effect for a series of pyridine-containing probe molecules. Finally, the enhancement mechanism is discussed according to the CT contribution. As a promising substrate for SERS technology, CH 3 NH 3 PbBr 3 will provide more possibilities for quantitative and multiplex detection in the future.
研究领域:
分析化学
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材料科学
·
光谱学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Hui Ma,Christian Marcus Pedersen,Qi Zhao,Zexiang Lyu,Hui Chang,Yan Qiao,Xianglin Hou,Yingxiong Wang
The Fischer–Tropsch (FT) process is a practical approach to convert synthesis gas (CO and H 2 ) into hydrocarbons and oxygenates, and these product mixtures are usually well-characterized. However, the analysis of Fischer–Tropsch waste water (FTW) is still somewhat underdeveloped and the exact composition of FTW remains unclear. Herein, various qualitative NMR techniques, especially diffusion-ordered spectroscopy (DOSY) and one dimension (1D) selective gradient total correlation spectroscopy (SelTOCSY) were strategically applied in the analysis of FTW. The NMR results show that the DOSY technique can pseudo-separate most of components in complex mixtures over the diffusion dimension. The SelTOCSY technique is used as a supporting method in the cases where the DOSY technique cannot clearly distinguish overlapped signals. Moreover, the quantitative 1 H NMR (qNMR) was further used to quantify the components of the sample. These routine and advanced qualitative and quantitative NMR technique utilized here provide a fast, effective and feasible method for the identification of complex mixtures in FTW, which might be a powerful and fast alternative to gas chromatography or high performance liquid chromatography for FTW research.
研究领域:
化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF RARE EARTHS
·
2023
·
IF 4.9
Songil Sin,Jiawei Ji,Lijun Cheng,Zhiwen Gu,Chong Tan,Kwangchol Ri,Changjin Tang
CeO 2 -based catalysts are widely investigated for selective catalytic reduction (SCR) of NO with NH 3 . Interaction of NO/O 2 with CeO 2 generally produces two surface species, i.e., nitrates and nitrites. However, the explicit quantification of these two species is still unresolved. Herein, we reported that spectrophotometry characterization was effective in determining surface adsorbed NO x species on CeO 2 by measuring the corresponding ions (NO 2 – and NO 3 – ) dissolved in aqueous solution. Experimental results show that both nitrate (–NO 3 ) and nitrite (–NO 2 ) species can be quantitatively evaluated and the accuracy is verified by calibrating with NO x -TPD result. Exclusive transfer of adsorbed NO x from catalyst surface to aqueous solution is confirmed and the dissolution process can be accelerated by ultrasonic treatment. Moreover, useful information related to evolution of surface NO x species under various conditions (O 2 treatment, different adsorption temperature and duration) and over different catalysts (Fe 2 O 3 , MnO 2 and MnO x –CeO 2 ) are provided. The result of present study demonstrates the potential of spectrophotometry for quantitative discrimination of surface NO x species on CeO 2 and other oxide-based materials, which is conducive to mechanism analysis of SCR reaction.
研究领域:
催化化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）