CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jinhua Liu,Lan Guan,Zhe Li,Li Huang,Dan Li,Zhe Li,Xiaohui Wang,Yingqun Zhu,Yuanan Liu
Real-time blood gas analysis is of great significance in disease diagnosis and treatment. Here we have developed a pH, PO 2 and PCO 2 multifunctional fluorescent capillary sensing system. The pH, O 2 and CO 2 fluorescent indicators with same excitation wavelength are fixed into a single capillary tube. The sensing system possesses sensitive fluorescence response to pH, O 2 and CO 2 , and the changes of pH, O 2 and CO 2 in buffer, in vitro, and in vivo can be detected simultaneously by measuring the fluorescence signals of sensor probe. The PO 2 , PCO 2 and pH of rabbits in four disease models were assessed efficiently by the sensing system, exhibiting similar variation trend with the results by blood gas analyzer. Studies in living rabbit display the reasonable and favorable feasibility of capillary sensing system in real-time arterial blood gas analysis. Overall, the multifunctional fluorescent capillary sensing system exhibits significant potential in real-time arterial blood gas analysis and clinical application of disease diagnosis.
研究领域:
医学诊断
·
生物传感技术
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Fangbing Wang,Jing Ren,Qiwen Peng,Hongyi Sun,Qiankun Zeng,Yongheng Zhang,Guoyue Shi,Min Zhang
Plasma separation, rich in biomarkers crucial for diagnosis, is conventionally achieved via high-speed centrifugation, a method hindered by its blood usage, lengthy processes, and complex operations, which delays detection. We introduced a novel real-time blood sensing method based on a Janus membrane and enzymes @MOFs. Asymmetric driving of the janus membrane can realize spontaneous separation of plasma and prevent hemolysis during direct separation. Glucose oxidase (GOx), uric acid oxidase (UOx) and horseradish peroxidase (HRP) were encapsulated in a hydrophilic organometallic framework (MOFs) to construct an enzyme cascade nanoreactor. Embedding enzyme in hydrophilic MOFs not only retains the natural conformation of free enzyme but also improves the brittleness of enzyme, endows MOFs with new biological functions, and expands its sensing application. Using 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogen and a custom app for color interpretation, we achieved real-time visualization of glucose (Glu) and uric acid (UA) at a 50 μM limit. The system accurately analyzed serum samples, matching commercial kits and showing promise for portable, personalized diagnostics.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Kai-Yue Tan,Chun-Yan Li,Yong-Fei Li,Junjie Fei,Bin Yang,Ya-Jun Fu,Fang Li
Adenosine triphosphate (ATP) is mainly produced in the mitochondrion and used as a universal energy source for various cellular events. Various fluorescent probes for ATP have been established successfully, but most of them are not appropriate for monitoring the fluctuation of the mitochondrial ATP level. Herein, a fluorescent probe named Mito-Rh is first synthesized and used to recognize ATP in mitochondrion. In the probe, rhodamine, diethylenetriamine, and triphenylphosphonium are selected as fluorophore, reaction site, and mitochondrion-targeting group, respectively. Probe Mito-Rh shows high sensitivity to ATP with 81-fold fluorescence enhancement, and the detection range (0.1–10 mM) can match the concentration level of ATP in the mitochondrion. Moreover, Mito-Rh provides excellent selectivity toward ATP over other biological anions (ADP, AMP, GTP, CTP, UTP) owing to a concurrent effect of dual recognition sites (hydrogen bond and π–π stacking). In particular, the probe can localize in mitochondrion specifically and demonstrates utility in the real-time detection of mitochondrial ATP concentration changes.
研究领域:
细胞生物学
·
生物化学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 18
·4379-4386
·
IF 3.307
Wang Hong,Zhang Peisheng,Tian Yong,Zhang Yuan,Yang Heping,Chen Shu,Zeng Rongjin,Long Yunfei,Chen Jian
A simple and readily available fluorescent probe is needed for the real-time monitoring of endogenous cysteine (Cys) levels in living cells, as such a probe could be used to study the role of Cys in related diseases. Herein, we report the first fluorescent probe based on carbon dots (CDs-FITA) for the selective and ratiometric imaging of endogenous Cys in live cells. In this ratiometric fluorescent probe, a fluorescein derivative (FITA) that recognizes Cys is covalently linked to the surfaces of carbon dots (CDs); employing CDs greatly improves the water solubility of the probe. Acrylate on FITA is selectively cleaved by Cys in aqueous solution under mild conditions, leading to a dramatic increase in the fluorescence from fluorescein. The probe therefore allows the highly selective ratiometric fluorescent detection of Cys even in the presence of various interferents. The as-prepared CDs-FITA showed excellent performance when applied to detect Cys in blood serum. In addition, due to its negligible cytotoxicity, the CDs-FITA can also be utilized for the real-time monitoring of endogenous cysteine (Cys) levels in living cells.
Illustration of the CD-based probe for Cys imaging in living cells
研究领域:
生物化学
·
分析化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Jiameng Pei,Lina Tang,Chenyang Kang,Fan Shi,Fengxia Liang,Yutao Li,Guo-Jun Zhang
Acupuncture therapy reduces blood pressure in hypertensive patients by stimulating acupoints located at specific anatomical positions with needles. Hydrogen sulfide (H 2 S) release and accumulation in the paraventricular nucleus (PVN) might be a pivotal step for triggering blood pressure reduction. But to date, the dynamics of H 2 S in the PVN during acupuncture treatment remain unclear. In this work, a multiple-nanomaterial-functionalized microneedle sensor is prepared for real-time monitoring of H 2 S in the PVN of rat while stimulated by electroacupuncture. Gold nanoparticles (AuNPs), molybdenum disulfide (MoS 2 ) and Ag 2 S were sequentially layered on the needle surface. The excellent electrical conductivity of AuNPs, the high carrier mobility of MoS 2 and the high catalytic performance of Ag 2 S ensure sensitive and specific detection of H 2 S in the range of 1 μM–100 μM with detection limit down to 95 nM. The microneedle sensor also demonstrates exceptional stability, remaining effective at room temperature for up to two weeks. Based on this, the microneedle sensor is used to monitor H 2 S release in vivo before and after acupuncture treatment. Our research indicates that acupuncture effectively increases H 2 S levels in the PVN of hypertensive rats, contributing to blood pressure reduction. This microneedle sensor provides a reliable method for monitoring H 2 S release in vivo, offering valuable insights into the role of H 2 S in the neurophysiology of hypertension and the mechanism of acupuncture in its treatment.
研究领域:
神经生理学
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生物传感技术
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中医药学
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产品关联
DOI
PubMed
高影响因子
Nature Protocols
·
2024
·
IF 13.1
Ma Xiaoxie,Zeng Xiaoyan,Huang Yurou,Liu Sheng Hua,Yin Jun,Yang Guang-Fu
Salt stress is an adverse environmental condition that harms plant growth and development. The development of salt stress probes is critical for tracking the growth dynamics of plants, molecular breeding or screening of growth regulators. The sodium chloride (NaCl)-responsive fluorescent probe Aza-CyBz is designed based on the tenet that NaCl induces formation of ordered aggregates, and the sensitive fluorescence response can enable the visualization of plant salt stress in root tip tissues and live plants. Herein, we describe a detailed three-step route for synthesis of Aza-CyBz and applications to monitoring salt stress in Arabidopsis thaliana . The procedures for operating fluorescence imaging under various stresses are also listed to eliminate interference from the oxidative mechanism of salt stress. Compared with conventional invasive approaches such as inductively coupled plasma emission spectrometry and flame photometer, our protocol can real-time monitor salt stress experienced by plants, which demands simple pretreatment procedure and staining technique. Due to near infrared fluorescence, this method provides direct visual observation of salt stress at both tissue and live plant levels, which is superior to conventional noninvasive approaches. The preparation of probe Aza-CyBz takes ~2 d, and the imaging experiments for assessing salt stress experienced by plants, including the preparation of stressed plant samples takes ~9–11 d for root tip tissues and ~23 d for live plants. Notably, acquisition and analysis visual images of salt stress in plants can be completed within 2 h and they require only a basic knowledge of spectroscopy and chemistry.
研究领域:
植物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Advanced Optical Materials
·
2025
·
IF 7.2
Jie Xu,Yanyan Li,Yuge Yao,Yanjun Ding,Yaying Tan,Gaoji Hu,Shiming Zhang,Lintao Zeng
In nature, the ability to regulate and transmit color signals is fundamental to the adaptive survival of many organisms. A key aspect of this adaptability is the autonomous detection of chromatic changes in color-displaying structures, facilitating precise color matching with environmental stimuli. However, conventional mechanochromic materials rely on a single mechanical force feedback channel, lacking the ability to continuously monitor their color state in real-time. In this work, a novel approach is presented that integrates ZnS@SiO 2 photonic crystals and LiCl into a TEMPO-Oxidized cellulose nanofibers-polyacrylamide hydrogel matrix (PAM-TOCNF hydrogel), which successfully endow conductivity variation into this mechanochromic hydrogel. This system mimics the chameleonic mechanism by coupling mechanical force with ion conduction to achieve responsive color changes and real-time color process monitoring. These findings establish a new paradigm for optoelectronic systems with stimulus-responsive capabilities, paving the way for future advancements in bio-inspired, adaptive technologies.
研究领域:
材料科学
·
生物工程
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Rui Liao,Xiaosong Du,Zhiqiang Zhai,Yang Wang,Jun Li,Yin Long,Yadong Jiang,Heng Zheng
Excessive or burst generation of reactive oxygen species (ROS) can induce oxidative stress, precipitating a range of critical illnesses, including cancers, Parkinson's disease and Ischemia-reperfusion injury. Conventional biological assays for ROS, involving discrete steps of capturing, labelling, and spectrometric detection, are complex and time-intensive. Moreover, their accuracy is substantially compromised by the short lifespan (microseconds to milliseconds) of ROS. Consequently, there is a pressing need for a rapid and efficient method that enables real-time detection. In this study, we have developed a printable, flexible ROS sensor based on a robust nanoenzyme composite by direct deposition of the paste onto a flexible polyethylene terephthalate (PET) substrate. This device demonstrated the fast and real-time responses to the hydrogen peroxide (mimetic agent) in the laboratory and to total ROS in sweat of an individual, exhibiting an outstanding current response to hydrogen peroxide across a broad concentration range of 0.01–10 mM, with a limit of detection (LOD) of 1.85 μM. The device's sensitivity to hydrogen peroxide (136.59 μA mM −1 cm −2 ), was found to be 1.5 to 10 times higher than that of sensors previously reported. Moreover, the IFRS device successfully identified instantaneous ROS levels in the sweat of adult males in vitro , with amperometric response increased 8 times after half an hour strenuous exercise, thereby exhibiting excellent selectivity, remarkable stability, and confirmed high biosafety. Overall, the IFRS provides a viable and practical solution for simple, expedited, and real-time ROS detection in the near future.
研究领域:
生物医学工程
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分析化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CELL BIOLOGY
·
2024
·Vol. 224
·期 1
·e202403198
·
IF 7.4
Liu Xiaofan,Chang Zhi,Sun Pingping,Cao Beibei,Wang Yuzhi,Fang Jie,Pei Yechun,Chen Baohui,Zou Wei
Maximizing cell survival under stress requires rapid and transient adjustments of RNA and protein synthesis. However, capturing these dynamic changes at both single-cell level and across an organism has been challenging. Here, we developed a system named MONITTR (MS2-embedded mCherry-based monitoring of transcription) for real-time simultaneous measurement of nascent transcripts and endogenous protein levels in C. elegans. Utilizing this system, we monitored the transcriptional bursting of fasting-induced genes and found that the epidermis responds to fasting by modulating the proportion of actively transcribing nuclei and transcriptional kinetics of individual alleles. Additionally, our findings revealed the essential roles of the transcription factors NHR-49 and HLH-30 in governing the transcriptional kinetics of fasting-induced genes under fasting. Furthermore, we tracked transcriptional dynamics during heat-shock response and ER unfolded protein response and observed rapid changes in the level of nascent transcripts under stress conditions. Collectively, our study provides a foundation for quantitatively investigating how animals spatiotemporally modulate transcription in various physiological and pathological conditions.
研究领域:
分子生物学
·
遗传学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Zhongjiao Feng,Jiasheng Wu,Meiyu Jiang,Jie Sha,Weimin Liu,Haohui Ren,Wenjun Zhang,Chun-Sing Lee,Pengfei Wang
The pH homeostasis of Golgi plays a vital role in maintaining normal cell functions. An effective near-infrared fluorescent probe that can sense pH changes in Golgi apparatus is of great significance. Here, a Golgi-targeting fluorescent probe ( Golgi-hNR) based on a rhodamine derivative is presented to show a highly sensitive response to pH variations. Golgi-hNR displays a conspicuous pH-dependent behavior at 664 nm from pH 7.4–3.0 with a remarkable fluorescence off-on response. The pKa value of Golgi-hNR is measured to be 3.62. Confocal fluorescence imaging reveals that Golgi-hNR diffuses into Golgi with high selectivity . Using Golgi-hNR , we have successfully visualized real-time tracking of Golgi pH variations for medical therapy in living cells.
研究领域:
细胞生物学
·
医学
✓ 已验证
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2021
·
IF 5.275
Yingying Zhang,Yanliang Zhao,Bo Song,Kunming Liu,Jiamin Gu,Yiying Yue,Ranhua Xiong,Chaobo Huang
Fluorescence probe combines with fluorescence imaging technology has become the most powerful analytical method with their great advantages of high sensitivity and selectivity and real-time monitoring. Ni 2+ is widely distributed in food, environment and living animals, thereof, it is of great significance for detection Ni 2+ with high selectivity. Herein, a simple strategy is proposed to design and synthesiz a small molecule fluorescent probe Y1 by using “one-pot” method. The spectroscopic behaviors including UV–Vis absorption and fluorescence emission spectrum have been used to verify the feasibility of probe towards Ni 2+ in water/EtOH ( v / v = 2:8) mixtures under neutral condition. As expected, Y1 offers high selectivity and sensitivity for detection Ni 2+ in aqueous solution with a good linear relationship and low detection limit within Ni 2+ concentration variation from 0 to 13 μM ( DOL = 0.0038 μM, R 2 = 0.9983). It is remarkable that Y1 can be applied for real-time visualization Ni 2+ change in sprouted potato and zebrafish with great photo-stability, highlighting that the practicability and feasibility of Y1 to detect and monitor Ni 2+ in the field of food industry and biomedical field.
研究领域:
分析化学
·
生物医学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS
·
2017
·Vol. 17
·期 3
·530
·
IF 2.677
Ru-Jia Yu,Jia-Jia Sun,Heng Song,Jing-Zhi Tian,Da-Wei Li,Yi-Tao Long
Real-time monitoring of chemical reactions is still challenging as well as important to study reaction mechanisms and reaction kinetics. Herein, we demonstrated the real-time monitoring of o-phenylenediamine (OPD) oxidation on the surface of gold nanoparticles by surface-enhanced Raman spectroscopy (SERS). The oxidation mechanism and the reaction kinetics were investigated on the basis of the SERS spectrum variation and the related density functionalized theory calculation. It was shown that the oxidation of OPD in the presence of copper ions was a two-step process of the deprotonation of the amino group on the aromatic rings and the rearrangement of the electron cloud to a π-conjugated system, which may open a new door to comprehensively understand the reaction process.Keywords:surface-enhanced Raman spectroscopy;oxidation of o-phenylenediamine;real-time monitoring
研究领域:
化学动力学
·
理论计算
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）