Insects
·
2024
·Vol. 16
·期 1
·31
·
IF 2.7
Shaohua Lu,Li Yang,Zonglin Wu,Mingshun Chen,Yujie Lu
Simple SummaryThe sense of smell helps insects perform essential behaviors like finding mates and food and avoiding dangers. Predators use unique smells to locate prey, but it is less understood if prey can recognize these predator odors and use them to escape. In this study, we examined howTribolium castaneum(Coleoptera: Tenebrionidae) andOryzaephilus surinamensis(Coleoptera: Silvanidae), two common pests, react to the predatorXylocoris flavipes, widely used in pest control. We found that these pests avoidX. flavipesodors, particularly the volatiles, linalool and geraniol, which decrease their attraction to food sources. These compounds show strong potential as natural repellents for pest management.The olfactory sensory system plays vital roles in daily activities, such as locating mate partners, foraging, and risk avoidance. Natural enemies can locate their prey through characteristic volatiles. However, little is known about whether prey can recognize the volatiles of their predators and if this recognition can increase the efficiency of prey escaping from predators.Xylocoris flavipesis a predator ofTribolium castaneum(Herbst) andOryzaephilus surinamensis(Linne) that has been widely used in stored pest control. Herein, we analyze the volatile components ofXylocoris flavipesand their impacts on the olfactory behavior ofT. castaneumandO. surinamensis. We found thatT. castaneumandO. surinamensispreferred blank air rather than odors ofX. flavipesandX. flavipesemissions, which significantly decreased the orientation preference ofT. castaneumandO. surinamensisto wheat.X. flavipesemits three major volatiles, including linalool, α-terpineol, and geraniol. Y-tube bioassays showed thatT. castaneumandO. surinamensiscan recognize linalool and geraniol at certain concentrations, especially at 200 μg/mL. EAG recordings verified that linalool and geraniol elicit higher olfactory responses in the two pests, but very small EAG responses were observed in the insects to α-terpineol. A further repellency evaluation also proved that linalool and geraniol are repellent to the two pests, and this repellency can be slightly enhanced by mixing them together.T. castaneumandO. surinamensiscan recognize the predatorX. flavipesby perceiving its volatiles and using them as signals for escaping. The two most potent volatiles, linalool and geraniol, may have potential values as repellents in controlling pests in these two stored products.
研究领域:
昆虫学
·
化学生态学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Insects
·
2021
·Vol. 12
·期 10
·870
·
IF 2.769
Han Chen,Honghua Su,Shuai Zhang,Tianxing Jing,Zhe Liu,Yizhong Yang
Simple SummarySince the widespread adoption of Bt cotton in the late 1990s, the green mirid bug,Apolygus lucorum(Hemiptera: Miridae), has become one of the most important pests in cotton fields and some other crops. To manage this destructive pest,Peristenus spretus(Hymenoptera: Braconidae) has been tested in augmentative biological control. In this study, after cotton plants were damaged by different densities ofA. lucorum, the behavioral responses ofA. lucorumandP. spretusto cotton plants volatiles were evaluated, and the quality and quantity of volatiles from cotton plants were analyzed. The results demonstrated that HIPVs emitted by plants in response toA. lucorumcould be influenced by the pest density and could be identified byP. spretusas a signal of the host. Our results would help understand howP. spretusplays a role in biological control againstA. lucorum.AbstractPlants would release herbivore-induced plant volatiles (HIPVs) to repel herbivores and attract natural enemies after being damaged by herbivores. In this study, after cotton plants were damaged by different densities ofApolygus lucorum, the behavioral responses ofA. lucorumandPeristenus spretusto cotton plants volatiles were evaluated, and the quality and quantity of volatiles from cotton plants were analyzed. Only when cotton plants were damaged by four bugs did bothA. lucorumandP. spretusshow an obvious response to damaged cotton plants, which indicates that cotton defense is correlated with pest density. The collection and analysis of volatiles reveals that the increase in pest density results in the emission of new compounds and an increase in the total number of volatiles with an alteration in proportions among the compounds in the blend. These changes in volatile profiles might provide wasps and mirids with specific information on host habitat quality and thus could explain the behavioral responses of parasitoids and pests.Keywords:behavior;pest;natural enemy;HIPVs;density
研究领域:
昆虫学
·
植物保护
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Huitang Qi,Tian Liu,Qiong Lu,Qing Yang
Cycloxaprid (CYC) is effective in the control of hemipteran pests, but its bioactivity against lepidopteran pests is still unclear. Here, the bioactivity of CYC against lepidopteran pests was found to be much worse than that against hemipteran insects. To reveal the mechanism, the transcriptomes of CYC-treated and untreated Ostrinia furnacalis larvae were compared. Among the top 20 differentially expressed genes, 11 encode proteins involved in cuticle formation, while only one encodes a detoxifying enzyme. Thus, the cuticle appears to be important for the insensitivity of O. furnacalis to CYC. A pretreatment of O. furnacalis larvae with methoprene enhanced the bioactivity of CYC by 1.12-fold. Moreover, mixtures of CYC with graphene oxide increased the bioactivity of CYC by 1.88-fold. Because lepidopteran and hemipteran insects often harm crops at the same time, the work can help make full use of CYC and reduce the environmental impacts of using multiple pesticides.
研究领域:
昆虫学
·
农药科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2025
·
IF 6.3
Lanxin Luo,Ting Gao,Yanni Deng,Mengyao Chai,Bo Li,Hao Ni,Kai Wang,Mengting Zhang,Yaojia Liu,Hao Jiang,Chuankui Song,Tingting Jing
Herbivore-induced plant volatiles play a crucial role in enhancing plant resistance and adaptability. However, the key volatiles triggering this response and their activation mechanisms remain largely unknown. Although tea aphids ( Toxoptera aurantii ) are common tea plantation pests, the underlying biochemical pathways by which aphid-induced volatiles in Camellia sinensis contribute to aphid resistance are not well understood. In this study, we measured jasmonic acid (JA) accumulation induced by aphid feeding using LC-MS and DESI-IMS analysis. We also identified a diverse array of aphid-induced volatiles through GC-MS. Notably, tea aphids stimulated β-Elemene accumulation, enhancing ‘Shuchazao’ resistance to tea aphids. We also evaluated the insecticidal activity of β-Elemene through contact toxicity and repellent bioassays. For the first time, we identified aphid-induced CsELE as a terpene synthase enzyme catalyzing the final step in β-Elemene biosynthesis from farnesyl pyrophosphate. Transient overexpression and gene silencing of CsELE affected β-Elemene accumulation, thereby influencing tea plant resistance to aphids. Furthermore, Dual-Luciferase and Y1H assays revealed that MYC2a, a core transcription factor in the JA signaling pathway, positively regulates CsELE expression. Overall, this study advances our understanding of the transcriptional regulation of tea volatiles in response to pest attacks, providing new insights into the ecological significance of terpene volatiles under biotic stress.
研究领域:
植物学
·
分子生物学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CELLULAR PHYSIOLOGY
·
2021
·Vol. 236
·期 8
·5921-5936
·
IF 6.384
Sijia Liang,Jing Luo,Muna Alariqi,Zhongping Xu,Aoli Wang,Muhammad Naeem Zafar,Jun Ren,Fuqiu Wang,Xuefei Liu,Yanfeng Xin,Haonan Xu,Weifeng Guo,Yanqin Wang,Weihua Ma,Lizhen Chen,Keith Lindsey,Xianlong Zhang,Shuangxia Jin
Plant bugs (Miridae species) have become major agricultural pests that cause increasing and severe economic damage. Plant-mediated RNA interference (RNAi) is emerging as an eco-friendly, efficient, and reliable strategy for pest management. In this study, we isolated and characterized a lethal gene of Apolygus lucorum and named it Apolygus lucorum LIM ( AlLIM ), which produced A. lucorum mortality rates ranging from 38% to 81%. Downregulation of the AlLIM gene expression in A. lucorum by injection of a double-stranded RNA (dsRNA) led to muscle structural disorganization that resulted in metamorphosis deficiency and increased mortality. Then we constructed a plant expression vector that enabled transgenic cotton to highly and stably express dsRNA of AlLIM ( dsAlLIM ) by Agrobacterium -mediated genetic transformation. In the field bioassay, dsAlLIM transgenic cotton was protected from A. lucorum damage with high efficiency, with almost no detectable yield loss. Therefore, our study successfully provides a promising genetically modified strategy to overpower A. lucorum attack.
研究领域:
植物保护
·
分子生物学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
Insects
·
2025
·Vol. 16
·期 3
·307
·
IF 2.9
Fida Hussain Magsi,David R. Hall,Zongxiu Luo,Xiangfei Meng,Chunli Xiu,Zhaoqun Li,Lei Bian,Nanxia Fu,Jianlong Li,Zongmao Chen,Xiaoming Cai
Simple SummaryHelopeltis cinchonaeis an economically important pest of tea plantations in Asia and has recently been reported in various provinces of China, expanding its population range. The bugs feed on growing shoots, sucking the sap and leaving the shoots susceptible to infection by fungi. No known control or monitoring tools have been developed forH. cinchonaeexcept for the excessive application of pesticides. The components of the sex pheromone produced by femaleH. cinchonaehave been identified and synthesized in previous work and have been shown to attract male bugs to traps. This is the first time that the components of the sex pheromone have been identified for a species ofHelopeltisbug. Here, we present the results of field trapping tests to optimize the blend of pheromone components, compare different types of traps, and determine the best position of the trap in order to maximize the catch of male bugs. Traps baited with the optimized lure were used to monitor populations of the bug and showed two distinct generations during the year. These results contribute to an environmentally-friendly approach to monitoring and managingH. cinchonaein the field.Helopeltis cinchonaeis an emerging pest of tea and causes severe damage to tea plantations in China. The female ofH. cinchonaehas been reported to produce a sex pheromone consisting of two components, hexyl (3R)-3-acetoxybutyrate and (5R)-1-acetoxy-5-butyroxyhexane, and lures containing the synthetic compounds have been shown to attract maleH. cinchonaeto traps in the field. This is the first time that components of the sex pheromone have been identified for a species ofHelopeltisbug, but their field application has not been evaluated in detail. The present study shows that a blend of both compounds loaded into a polyethylene vial at 0.2 and 2 mg, respectively, caught significantly more maleH. cinchonaebugs than the individual compounds and all the other tested blends. Sticky wing traps baited with the binary blend of compounds at the optimized ratio and dosage caught more bugs than bucket funnel or delta traps, and traps hung at a height of 10 cm above the tea shoots caught more bugs than those at other heights. The optimized traps and lures were used for monitoring the pest and two distinct population peaks ofH. cinchonaewere observed, the first one during mid-May to early June, and the other one in mid-September. Overall, the results of this study contribute to an environmentally-friendly approach to monitoring and managingH. cinchonaein the field.
研究领域:
昆虫学
·
农业科学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED ENTOMOLOGY
·
2012
·Vol. 137
·期 6
·462-468
·
IF 1.311
P Gu,C.-L. Peng,J. Li,C.-H. Feng,P. Li,Y.-J. Du
Field studies using the synthetic sex pheromone of Trichophysetis cretacea , a trinary blend of ( Z )-11-hexadecenyl acetate ( Z 11-16:OAc), ( Z )-11-hexadecenal ( Z 11-16:Ald) and ( Z )-11-hexadecenol ( Z 11-16:OH), were performed in Sichuan to determine operational parameters for detection and control, such as dispenser type, blend ratio, dosage, and trap type, height and density. Of three pheromone dispensers tested, grey halo-butyl isoprene elastomeric septa were significantly more effective than polyvinyl chloride capillary tubing or silicone rubber septa. The ratio of the three components in the blend significantly affected moth catch. In the halo-butyl isoprene septa, the most effective ratio was 5 : 2 : 1 Z 11-16:OAc: Z 11-16:Ald: Z 11-16:OH. Sticky wing traps caught significantly more moths than water, noctuid moth or cone funnel traps. The most effective height at which wing traps were hung was 20 cm above the jasmine plants. Optimum trap density was 45 traps per hectare. Addition of volatile jasmine compounds did not increase the attractiveness of the sex pheromone. A dosage of 50 μg Z 11-16:OAc per lure was most effective in the autumn weather conditions of Quanwei. These data provide sufficient information to develop effective protocols for using the T. cretacea pheromone to detect and monitor this pest in the jasmine fields.
研究领域:
昆虫学
·
农业害虫防治
✓ 已验证
DOI
高影响因子
PLANT PHYSIOLOGY
·
2017
·Vol. 176
·期 2
·1808-1823
·
IF 6.456
Hu Qin,Min Ling,Yang Xiyan,Jin Shuangxia,Zhang Lin,Li Yaoyao,Ma Yizan,Qi Xuewei,Li Dongqin,Liu Hongbo,Lindsey Keith,Zhu Longfu,Zhang Xianlong
Plants are constantly challenged by a multitude of pathogens and pests, which causes massive yield and quality losses annually. A promising approach to reduce such losses is to enhance the immune system of plants through genetic engineering. Previous work has shown that laccases (p-diphenol:dioxygen oxidoreductase, EC 1.10.3.2) function as lignin polymerization enzymes. Here we demonstrate that transgenic manipulation of the expression of the laccase gene GhLac1 in cotton (Gossypium hirsutum) can confer an enhanced defense response to both pathogens and pests. Overexpression of GhLac1 leads to increased lignification, associated with increased tolerance to the fungal pathogen Verticillium dahliae and to the insect pests cotton bollworm (Helicoverpa armigera) and cotton aphid (Aphis gosypii). Suppression of GhLac1 expression leads to a redirection of metabolic flux in the phenylpropanoid pathway, causing the accumulation of JA and secondary metabolites that confer resistance to V. dahliae and cotton bollworm; it also leads to increased susceptibility to cotton aphid. Plant laccases therefore provide a new molecular tool to engineer pest and pathogen resistance in crops.
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 3
·631
·
IF 4.6
Xu Feng,Cheng-Bin Shan,Jian-Nan Ma,Yue Ma,Na Li,De-Jian Zhang,Zhan-Yuan Lu,Chao-Mei Ma
Tribolium confusum is a major stored-product pest that exhibits resistance to chemically synthesized pest repellents. This study investigated the potential of essential oil (EO) extracted from the roots of Cyperus esculentus as a natural alternative for pest management. The EO was obtained through steam distillation, and its chemical composition was elucidated using gas chromatography–mass spectrometry. The primary compounds, cyperotundone and cyperene, were further isolated from the EO through silica gel column chromatography. The efficacy of the EO and its isolated compounds as pest repellents was evaluated against a flaxseed pest, which was identified as T. confusum through DNA sequence analysis. The results demonstrated that at 86.12 μg/cm 2 , the EO and its two main components maintained significant repellent activity for up to 24 h. In contrast, the effectiveness of the positive control, N, N-diethyl-3-methylbenzamide (DEET) declined rapidly after 8 h. At 16 h, the repellent activity of the EO and one of its main components, cyperotundone, was significantly greater than that of DEET. Furthermore, at a lower concentration of 43.06 μg/cm 2 , cyperotundone’s repellent activity was significantly stronger than DEET’s at 16 h. Additionally, cyperotundone outperformed DEET significantly from 4 to 16 h at 21.53 μg/cm 2 and at 16 h at 10.76 μg/cm 2 . Among the two compounds, cyperotundone exhibited a longer-lasting repellent effect compared to cyperene, which is consistent with the lower evaporation rate of cyperotundone. Biochemical assays revealed that exposure to the EO of C. esculentus and its major compounds significantly reduced ( p < 0.05) the activities of acetylcholinesterase and glutathione-S-transferase in T. confusum . Molecular docking experiments indicated that the compounds could bind to olfactory receptors with low binding energies. qRT-PCR analysis revealed that the EO and its two compounds significantly altered ( p < 0.05) the expression levels of odorant receptor genes in the pest. These findings suggest that the repellent action of C. esculentus EO and its major compounds on T. confusum may be mediated through the modulation of the pest’s olfactory system, as well as by inhibiting essential enzymatic activities in the pests. This research contributes valuable insights into the development of sustainable, long-lasting, and eco-friendly pest repellents, harnessing the potential of the rich botanical resource C. esculentus .
研究领域:
植物学
·
昆虫学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2022
·
IF 5.437
Dahe Qiao,Mengsha Tang,Ling Jin,Xiaozeng Mi,Hongrong Chen,Junyan Zhu,Shengrui Liu,Chaoling Wei
Monoterpenes and sesquiterpenes are the most abundant volatiles in tea plants and have dual functions in aroma quality formation and defense responses in tea plants. Terpene synthases (TPS) are the key enzymes for the synthesis of terpenes in plants; however, the functions of most of them in tea plants are still unknown. In this study, six putative terpene biosynthesis gene clusters were identified from the tea plant genome. Then we cloned three new TPS-b subfamily genes, CsTPS08 , CsTPS10 and CsTPS58 . In vitro enzyme assays showed that CsTPS08 and CsTPS58 are two multiple-product terpene synthases, with the former synthesizing linalool as the main product, and β-myrcene, α-phellandrene, α-terpinolene, D-limonene, cis-β-ocimene, trans-β-ocimene and (4E,6Z)-allo-ocimene as minor products are also detected, while the latter catalyzing the formation of α-pinene and D-limonene using GPP as the substrate. No product of CsTPS10 was detected in the prokaryotic expression system , but geraniol production was detected when transiently expressed in tobacco leaves. CsTPS08 and CsTPS10 are two functional members of a monoterpene synthase gene cluster, which were significantly induced during both Ectropis oblique feeding and fresh leaf spreading treatments, suggesting that they have dual functions involved in tea plant pest defense and tea aroma quality regulation. In addition, the differences in their expression levels in different tea plant cultivars provide a possibility for the subsequent screening of tea plant resources with a specific aroma flavor. Our results deepen the understanding of terpenoid synthesis in tea plants.
研究领域:
植物学
·
分子生物学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·
IF 3.963
Shanshan Gao,Ruixue Lu,Yonglei Zhang,Haidi Sun,Siying Li,Kunpeng Zhang,Ruimin Li
Tribolium castaneum ( T. castaneum ) is a worldwide pest of stored grain that mainly harms flour, and not only causes serious loss of flour quality but also leads to deterioration of flour quality. Chemical detection plays a key role in insect behavior, and the role of odorant-binding proteins (OBPs) in insect chemical detection has been widely studied. However, the mechanism of OBPs in insect defense against exogenous toxic substances is still unclear. In this study, biochemical analysis showed that eugenol , the active component of A. vulgaris essential oil , significantly induced the expression of the OBP gene OBPC12 from T. castaneum ( TcOBPC12 ). The mortality of late larvae treated with eugenol was higher than that of the control group after RNA interference (RNAi) against TcOBPC12 , which indicates that the OBP gene is involved in the eugenol defense mechanism and leads to a decrease in sensitivity to eugenol. Tissue expression profiling showed that the expression of TcOBPC12 in the epidermis, hemolymph, and intestine was higher than in other larval tissues, and TcOBPC12 was expressed mainly in the epidermis, head, and fat body of adults. The developmental expression profile showed that the expression of TcOBPC12 in late eggs, early and late larval stages, and late adult stages was higher than in other developmental stages. These data suggest that TcOBPC12 may be involved in the absorption of exogenous toxic substances by the larvae from T. castaneum . Our results provide a theoretical basis for the metabolism and degradation mechanism of exogenous toxic substances and help explore more potential target genes of insect pests .
研究领域:
昆虫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
·
2020
·
IF 1.101
Yunhao Gao,Donglin Li,Dongyang Li,Pengfei Xu,Kaikai Mao,Yunhua Zhang,Xueying Qin,Tao Tang,Hu Wan,Jianhong Li,Mingcheng Guo,Shun He
Nanopesticides with antiwashing capacity on leaves are the most promising new approaches for sustainable pest management and have been fully evaluated in the laboratory. However, few studies have tested these nanopesticides on pests, and their efficacy under field conditions has not been investigated. In this study, an adhesive hollow mesoporous silica hybrid with well-defined spherical shape and good monodispersity was used as a nanocarrier of cyantraniliprole (CNAP) to fabricate an adhesive nanopesticide (CNAP-HMS-PDAAM). The control efficacy of CNAP-HMS-PDAAM was tested under field conditions. The results indicated that the efficacy of four doses of CNAP-HMS-PDAAM (30.0–69.0 g a.i./ha) against Cnaphalocrocis medinalis (Guénee) 3, 7, and 14 days after spraying did not significantly differ from that of Benevia (34.5 g a.i./ha). Twenty-eight days after spraying, the efficacy of all four doses of CNAP-HMS-PDAAM was significantly better than that of Benevia. Additionally, the efficacy of CNAP-HMS-PDAAM at doses of 34.5, 39.0 and 69.0 g a.i./ha against Chilo suppressalis (Walker) were significantly higher than that of Benevia (34.5 g a.i./ha). Thus, CNAP-HMS-PDAAM showed long-term control efficacies against C. medinalis (Guénee) and C. suppressalis (Walker), mainly due to its strong adhesive property on rice leaves and its sustained release properties. In addition, the nanocarriers showed good biocompatibility and had no obvious influence on the growth of rice.
研究领域:
农业科学
·
植物保护
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）