ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Lu huang,Hong Jiang,Bin Li,Lanying Chen,Tongtong Yang,Jiang Wang,Huanwen Chen
An online reactive internal extraction electrospray ionization (iEESI) method was developed for the rapid determination of organic and inorganic speciation information for selenium in poultry tissue samples without complex sample pretreatment. The addition of citric acid as a reducing agent to the internal extraction solvent of methanol/acetic acid (99:1, V/V) for iEESI resulted in the reduction of selenate in the sample to selenite, accompanied by the production of malic acid as an oxidation product. The quantitative analysis of selenate was conducted by using malic acid. By addition of o-phenylenediamine (OPD) to the extractant, inorganic selenium including selenite and selenite reduced from selenate was immediately extracted and converted into 1,3-dihydro-2,1,3-benzoselenadiazole after an in situ reaction. The methanol in the reagent extracted organic selenium compounds from the samples, enabling a rapid quantitative analysis of organic and inorganic selenium in chicken tissue. The concentrations of selenomethionine (SeMet), l-selenocystine (SeCys(2)), selenite, and selenate in different tissue samples were examined, showing strong linearity in the range of 1.00–100.00 μg/kg with the limit of detection (LOD) ranging from 0.36 to 0.84 μg/kg. The results were validated by two conventional methods with an accuracy of 84.2–107.6%. The quantitative analysis of three chicken visceral samples revealed that the highest concentration of organic selenium was present in chicken livers, while the highest concentration of inorganic selenium was observed in gizzards. The findings may provide a reliable method for studying selenium metabolism pathways in organisms.
研究领域:
分析化学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
VETERINARY PARASITOLOGY
·
2024
·
IF 2
Bohan Wang,Jiali Meng,Xiaoxiao Qi,Penglong Wang,Qi Liu,Lifang Wang,Weiwei Sun,Baoliang Pan
Surface hydrophobicity of organisms provides biological self-protection. The hydrophobicity of pest surface, acting as a main obstacle for the pest control, can lead to low utilization and high loss of pesticides. Dermanyssus gallinae is a notorious pest in egg-laying hens, whose control primarily depends on acaricide spraying, while its surface hydrophobicity and potential influence on pesticide effectiveness are not clear. In the present study, the contact angle measurements revealed that the surface of D. gallinae was hydrophobic. Analysis using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the surface microstructures of D. gallinae consist of cuticular folds, with a lipid-rich outermost layer of the cuticle. Based on gas chromatography-mass spectrometry (GC-MS) and gas chromatography (GC), it was found that the major compositions of cuticular lipids were fatty acids and n-alkanes. Modifying the chemical compositions and microstructures of the D. gallinae surface resulted in a reduction in surface hydrophobicity and an increase in the permeation of Rhodamine B through the cuticle. This observation suggested that the chemical compositions and microstructures were pivotal in determining surface hydrophobicity, hindering compound penetration into the cuticle. Finally, it was found improving the wettability of pesticide solution by adding surfactants could overcome the surface hydrophobicity and enhance the efficacy of pesticide against the mites. This study sheds light on the surface hydrophobicity mechanism of D. gallinae and provides a novel strategy to improve the efficacy of acaricides against the mites.
研究领域:
昆虫学
·
害虫防治
·
动物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2021
·Vol. 86
·期 12
·5495-5502
·
IF 3.167
Liangting Shao,Yue Qin,Xinglian Xu,Huhu Wang
Sodium hypochlorite (NaClO) has been widely used at 100 ppm concentration during poultry slaughter to reduce carcass microorganism loads. However, its use in poultry processing is restricted owing to the potential risks of disinfection by-products (DBPs) that can be produced by the reaction of NaClO with poultry meat components. This study assessed whether dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA), as primary DBP representatives, were produced when NaClO was used as a disinfectant in various methods during broiler prechilling. Headspace gas chromatography-mass spectrometry for the quantitative determination of DCAA and TCAA in 180 prechilling water samples and 30 broiler meat samples, obtained from large standard slaughterhouses equipped with an online monitoring system to control the NaClO concentration between 50 and 100 ppm, showed that neither DCAA nor TCAA were detected. In simulation assays, haloacetic acids (HAAs) were not detected when the concentration of the NaClO solution was less than 200 ppm with low frequency addition; however, more than 0.1 mg/L of DCAA and TCAA were detected on applying 200, 300, 400, 500, and 1000 ppm NaClO at high frequency. These findings indicated that adding high concentrations of NaClO and frequently adding low levels pose a potential risk of DBP formation. This investigation provides a basis for the establishment of food risk and the scientific use of NaClO in poultry processing, and contributes to the evaluation of DBPs in poultry slaughter. Practical Application This study confirmed the occurrences of DCAA and TCAA during broiler chilling processing, indicating that formation of HAAs in simulation systems was correlated with NaClO levels and validated the absence of DCAA and TCAA with less than 200 ppm, providing a basic study for food safety standards and regulations on the disinfectants used in food processing.
研究领域:
食品安全
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ZOOLOGISCHER ANZEIGER
·
2023
·
IF 1.4
Xiaoxiao Qi,Huan Li,Xin Liu,Bohan Wang,Jiali Meng,Qi Liu,Weiwei Sun,Baoliang Pan
Dermanyssus gallinae is a hematophagous ectoparasitic mite in the poultry industry worldwide. Its current control mainly relies on spraying acaricides with many deficiencies. It's urgently needed to develop novel control approaches, such as pheromones. The pheromones are detected by chemoreceptors , which are not well investigated in D. gallinae . In the current study, Y-tube olfactometer bioassays revealed that extracts of feces of mites could attract D. gallinae , indicating aggregation pheromones existed in feces. After varnishing the tips of the foreleg tarsi, the female mites' response to feces extracts disappeared in Y-tube olfactometer bioassays. Still, their response to guanine (a non-volatile compound acting as a potential arrestment pheromone) remained in filter paper contact bioassays. These results suggested that the sensilla at tips of the foreleg tarsi had olfactory function, and the sensilla at the tips of the pedipalpal tarsi could have gustatory function and the gustatory organs were located in other body parts of female mites. Through scanning electron microscopy (SEM), it was found that adults and nymphs possess a cluster of 8 peg-like sensilla as well as 2 blunt, peg-like sensilla at the tip of each pedipalpal tarsus. Nevertheless, the larvae had only a cluster of 5 blunt peg-type sensilla. Each sensillum has a terminal pore or depression, suggesting it functions as gustatory organ. The present study confirmed the location and explored the architecture of gustatory organs of D. gallinae , which would facilitate the development of novel control method based on pheromones .
研究领域:
昆虫学
·
寄生虫学
✓ 已验证
产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·Vol. 80
·期 10
·4950-4958
·
IF 3.8
Penglong Wang,Qi Liu,Xu Wang,Tiancong Sun,Boxing Liu,Bohan Wang,Huan Li,Chuanwen Wang,Weiwei Sun,Baoliang Pan
BACKGROUND Dermanyssus gallinae , the poultry red mite (PRM), is a worldwide ectoparasite posing significant economic challenges in poultry farming. The extensive use of pyrethroids for PRM control has led to the emergence of pyrethroid resistance. The objective of this study is to detect the pyrethroid resistance and explore its associated point mutations in the voltage-gated sodium channel (VGSC) gene among PRM populations in China. RESULTS Several populations of D. gallinae , namely CJF-1, CJP-2, CJP-3, CSD-4 and CLD-5, displayed varying degrees of resistance to beta-cypermethrin compared to a susceptible field population (CBP-5). Mutations of VGSC gene in populations of PRMs associated with pyrethroid resistance were identified through sequencing its fragments IIS4–IIS5 and IIIS6. The mutations I917V, M918T/L, A924G and L925V were present in multiple populations, while no mutations were found at positions T929, I936, F1534 and F1538. CONCLUSION The present study confirmed the presence of extremely high levels of pyrethroid resistance in PRM populations in China, and for the first time detected four pyrethroid resistance mutations in the VGSC gene. Identifying pyrethroid resistance in the field population of PRM in China can be achieved through screening for VGSC gene mutations as an early detection method. Our findings underscore the importance of implementing chemical PRM control strategies based on resistance evidence, while also considering the management of acaricide resistance in the control of PRMs. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
·
分子生物学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2024
·
IF 3.8
Xinzhang Chen,Xu Lin
Trimethyl tin (TMT) is a good stabilizer for plastic products but is also a toxic environmental pollutant. Selenium has good antioxidant and anti-inflammatory properties and has been widely used in the poultry industry. However, it has not been reported whether selenium enrichment can antagonize TMT-induced viral hepatitis in poultry. To fill this gap, AA broiler models exposed to TMT for 42 d were established and fed a Se-enriched diet at the same time. H&E staining showed that selenium could significantly alleviate TMT-induced liver inflammation. Further analysis of the underlying mechanism revealed that TMT induced nitrosation stress (RNS), increased NO content and iNOS expression, which in turn activated the NF-κB/NLRP3 pathway and induced pyroptosis. However, selenium enrichment can reverse this situation, that is, reduce the occurrence of RNS, reduce the degree of pyroptosis, and thus alleviate the occurrence of inflammation. In our study, we demonstrated for the first time that TMT could induce hepatotoxicity other than neurotoxicity in poultry and that selenium could antagonize TMT-induced hepatotoxic hepatitis in chickens through the RNS/NF-κB/NLRP3 pathway.
研究领域:
毒理学
·
兽医学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
VETERINARY PARASITOLOGY
·
2022
·
IF 2.821
Xiaoxiao Qi,Huan Li,Bohan Wang,Jiali Meng,Xu Wang,Weiwei Sun,Baoliang Pan
Dermanyssus gallinae , also known as the poultry red mite (PRM), is one of widespread ectoparasite in the poultry industry worldwide, causing direct and indirect detriments to poultry as well as substantial financial losses. Novel control methods are urgently needed to improve the current acaricide-based control of D. gallinae . The control approach based on arrestment pheromone is environment-friendly but the related research is limited in PRMs. In the present study we found two compounds from the mite feces acting as arrestment pheromones of D. gallinae , which could lead to mite arrestment upon contact. One is guanine, which was also found in unfed female mites’ acid-saline extract. The other is hematin. Moreover, it was found that the ferric ion of hematin played a pivotal role in stimulating the arrestment of mites. Finally, it was found the combination of guanine or/and hematin plus cypermethrin led to significantly improved mite-killing performance compared with cypermethrin, showing a promising potential of novel control method based on the arrestment pheromone.
研究领域:
昆虫学
·
寄生虫学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yi-Fan Xia,Yan-Xia Li,Qing-Yan Xiao,Zhi-Qiang Cai,Qian-Wen Yang,Yi-Xuan Hu,Zi-Yi Mei,Guang-Ming Bao,Hou-Qun Yuan
Diclazuril (DIC) is a broad-spectrum anti-coccidiosis drug of the triazine class, widely used in poultry farming. The overuse of DIC may lead to its accumulation in animal bodies, which may enter the food chain and threaten human health. In this work, we fabricated a stable Eu 3+ -doped UiO-66 fluorescence sensor ( EuUHIPA-30 ) for the sensitive detection of DIC. Among 20 veterinary drugs, the fluorescence of EuUHIPA-30 selectively responds to DIC, with a low detection limit (0.19 μM) and fast response (10 s). EuUHIPA-30 is recyclable and can detect DIC in chicken and eggs with good recoveries. Moreover, a smartphone-integrated paper-based sensor enables the instrument-free, rapid, visual, and intelligent detection of DIC in chickens and eggs. This work provides a promising candidate for practical fluorescent DIC sensing in animal-derived food to promote food safety.
研究领域:
食品安全
·
分析化学
·
兽医学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Hui Jin,Jie Hou,Yuyu Ruan,Jiang Xu,Kun Yang,Daohui Lin
Antibiotics are commonly released into paddy fields as mixtures via human activities. However, the simultaneous extraction and detection of these chemicals from multiple media are technically challenging due to their different physicochemical properties, resulting in unclear patterns of their transport in the soil-rice system. In this study, a “quick, easy, cheap, effective, rugged, and safe” (QuEChERS) method was developed for the simultaneous analysis of 4 tetracyclines (TCs) and 4 fluoroquinolones (FQs) in the soil and rice tissues from a local poultry farm, and thereby the distribution patterns of the target antibiotics in the soil-rice system and their risk levels to the soil were analyzed. After parameter optimization, the calibration range used for the target antibiotics was 0.1–50 μg/L and each calibration curve was linear with a coefficient of determination ( R 2 > 0.995); The QuEChERS method achieved satisfactory recovery rates (70.3-124.6%) along with sensitive detection limits (0.005-0.21 ng/g) for TCs and FQs in the soil, root, stem, leaf, and grain. Among the 8 antibiotics, enrofloxacin (ENX), ciprofloxacin (CIP), oxytetracycline (OTC), and doxycycline (DOX) were detected around a poultry farm. The four antibiotics in the collected paddy soils around the poultry farm ranged from 7.1 ng/g to 395.5 ng/g. Notably, ENX and DOX had higher ecological risks (risk quotient values >1) than CIP and OTC in soil. ENX, CIP, and DOX were highly enriched in rice roots with concentrations up to 471.9, 857.3, and 547.4 ng/g, respectively, which were also detected in rice aboveground tissues. The findings may provide both technical and practical guidance for the understanding of antibiotic environmental behavior and risks.
研究领域:
环境科学
·
农业化学
·
土壤学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）