Abstract In this study, flower-shaped nano-silica was prepared through a calcination method, and then modified. Then using the method of free radical polymerization, and polysiloxane composite compounded with siloxane. The prepared composite microspheres combined the stability of polysiloxane with the unique surface morphology of flower-shaped nano-silica. The relative density of the composite material was controlled by the significant difference in density between siloxane microspheres and nano-silica. The structure and morphology of the composite microspheres were characterized and analyzed using FTIR, SEM, and other tests. The results showed successful compounding of siloxane and nano-silica, resulting in superhydrophobic polysiloxane/nano-SiO 2 composite microspheres with a water contact angle of 152°. The composite microspheres exhibited a nebula-like overall structure.
Three-dimensional (3D) substrates have received considerable attention for the fabrication of protein microarrays with high protein capacity owing to their enlarged surface area for the high density immobilization of proteins. In this work, a facile strategy for constructing the protein microarrays with a low background and high signal intensity was developed based on the polystyrene nanoparticles (PS-NPs) assembled substrate. The discontinuous hydrophilic surface with hydrophobic initiators and hydrophilic poly(ethylene glycol) methacrylate (PEGMA) brushes was fabricated in the first step via digital micromirror device (DMD)-mediated photoinduced atom transfer radical polymerization (Photo-ATRP) process, and the hydrophilic interaction between sodium dodecyl sulfate (SDS)-modified PS-NPs and PEGMA brushes enabled the selective assembly of PS-NPs on the PEGMA brush region, resulting in the successful formation of arrayed surface with PS-NPs patterns. Then, poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) brushes were grafted surrounding the PS-NPs to eliminate background noise caused by non-specific protein absorption. Compared to the PGMA brushes patterned substrate, the PS-NPs substrate exhibited excellent protein adsorption capability, leading to the 3D protein microarrays with higher protein capacity were easily obtained. Especially, the assembly density could be controlled by adjusting the PS suspension concentration, resulting in the effective regulation on the density of surface-bound proteins. Finally, the spatial control of protein density on the same substrate was achieved through regulating the distribution density of assembled PS-NPs, allowing for a protein microarrays with controllable density. This simple and effective mothed for fabricating nanoparticle-based 3D protein microarrays has tremendous potential in the fields of biomedical detection and high-throughput analysis.
Cuiyan Li,Zhao Xu,Haibo Ouyang,Liyuan Chang,Jianfeng Huang,Yijun Liu
Porous C/SiC ceramics were prepared by chemical vapor reaction of SiO and porous C/C composites. The porous C/SiC ceramics presented a hierarchical structured consisted of carbon fibers, SiC layer, microspheres and nanowires. The density of porous C/C composites control the growth of SiC nanowires and nanolayer, and influence the pore size distribution and specific surface area of the porous C/SiC ceramics. The C/SiC samples converting from the C/C composites with a density of 0.46 g/cm 3 possess the superior adsorption properties. The adsorption efficiency can reach 98.2% within 80 min, indicating that C/SiC samples have a promising adsorption property for MB from aqueous solution. Furthermore, the adsorption efficiency after five cycles microwave regeneration still reached 80.2%, which present excellent regeneration stability.
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