ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Haoyang Liu,Yue Zhu,Weixu Chen,Renwang Sheng,Chuanquan Liu,Yuzhi Sun,Jia Liu,Mingyue Wang,Jun Lu,Jialin Chen,Wei Zhang
The advancement of nanomedicine requires a thorough understanding of the intrinsic bioactivity and molecular interactions of nanomaterials for safe and effective clinical applications, which remains lacking for most currently developed nanomaterials. Here, we uncover the unique intrinsic bioactivity and regulatory mechanisms of carbon-based fullerol nanomaterials through high-throughput molecular analysis and explore their therapeutic potential for tissue regeneration using tissue engineering approaches. Fullerol exhibits intrinsic pro-differentiation and antioxidant properties that enhance the osteogenesis and chondrogenesis of MSCs. Mechanistically, proteomic analysis combined with small-molecule inhibition studies reveals that fullerol is internalized by MSCs via clathrin-mediated endocytosis and activates NRF2 signaling, thereby exerting antioxidant effects that restore impaired MSC viability and differentiation under oxidative stress. Leveraging these unique bioactivities, we develop a fullerol-functionalized hydrogel with feasible physicochemical properties and triple biological functions in antioxidant, pro-osteogenic, and pro-chondrogenic effects and confirm its great regenerative capacity for both cartilage and subchondral bone by promoting structural restoration and improving functional recovery in a rat osteochondral defect model. Our findings offer new insights into the intricate interactions between stem cells and nanomaterials at the cellular and molecular levels and broaden the potential biomedical applications of fullerol for future cartilage and bone regeneration therapies.
研究领域:
再生医学
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纳米生物学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
Xiao-Pan Liu,Wen-Qian Sun,Tong-Xin Liu,Bing-Bing Liu,Chang-Po Chen
Due to the strong background interferences in the low-mass region and poor reproducibility of conventional organic matrices, it is of great importance to develop a novel matrix for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to qualitatively and quantitatively analyze small molecules. In this work, water-soluble fullerenol C 60 (OH) 24–26 was selected as a MALDI matrix for the analysis of low-molecular-weight compounds in consideration of optical absorption property, water solubility and stability. Compared with the traditional matrices, fullerenol demonstrated lower background interference and stronger peak intensity. In addition, the hydrophilic fullerenol could avoid the heterogeneous crystallization in sample preparation, increase the reproducibility and sensitivity of MALDI-MS, and ameliorate quantitative analysis of small molecules. With saccharin as model analyte, quantitative analysis was carried out using fullerenol as matrix. The results demonstrated satisfying reproducibility and good tolerance to salt. The limit-of-detection of the quantitative analysis was as low as 4 pmol, and the linear range is 1–100 μg mL −1 with R 2 greater than 0.99. The analytical results also showed excellent precision and accuracy, low matrix effect and good recovery rate. Fullerenol as a potential matrix was further validated in the quantification of saccharin sodium in different real food samples, such as nuts and drinks. This work not only confirms the potential of fullerenol for the quantitative analysis in food field, but also provides a new technique for rapid analysis of small molecules.
研究领域:
分析化学
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食品科学
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质谱技术
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DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2019
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IF 0
Mengjun Chen,Keyang Yin,Geping Zhang,Huizhong Liu,Bo Ning,Youyong Dai,Xiaojing Wang,Hongguang Li,Jingcheng Hao
Fullerene C60 (refers to C60 hereafter) has a unique three-dimensional architecture and intriguing physicochemical properties. It has great potential applications in materials chemistry and life science. However, a big obstacle for the widespread application of C60 lies in the limited strategies to make supramolecular structures with diverse morphologies and functions. Herein, we report a strategy to prepare C60-based, magnetic microcapsules which can be used as external antioxidants to effectively attenuate oxidative stress. The microcapsules are composed of fullerenol, a highly water-soluble C60 multiadduct, and iron ions (Fe3+) released from a rusty nail. They can be easily obtained through coordination between the hydrophilic functional groups in fullerenol and Fe3+ with polystyrene microspheres as templates. The fullerenol/Fe3+ microcapsules have good colloidal stability both in water and serum. Their biocompatibility has been confirmed by in vitro tests on HEK293 and Hela cells. Electron spin resonance measurements indicate that the fullerenol/Fe3+ microcapsules can effectively scavenge hydroxyl radicals (OH·–) produced by H2O2, which greatly improves the living environment of the cells. The fullerenol/Fe3+ microcapsules exhibit ferromagnetic properties and can respond to the external magnetic field, enabling magnetic manipulation, and/or separation in practical applications.
研究领域:
材料化学
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生命科学
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纳米医学
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DOI
PubMed
COMPOSITES COMMUNICATIONS
·
2024
·
IF 6.5
Fan Gao,Ying Nie,Yue Zhang,Tao Wang
Carbon nanomaterial is a momentous field of current biomedical research. However, extensive exploration is still required to endow carbon nanomaterial with both antibacterial properties and biological safety. In this study, fullerenol and calcium ions were synthesized into C 60 (OH) n -Ca nanoparticles through an electrostatic self-assembly strategy. Morphological and chemical analyses revealed that the C 60 (OH) n -Ca nanoparticles contained 4.52 at.% Ca, with a minimum size of approximately 10 nm. The electrostatic self-assembly did not alter the chemical structure of the fullerenol. The photodynamic antibacterial effect of the C 60 (OH) n -Ca nanoparticles was concentration-dependent, showing antibacterial rates of 94 % and 96.6 % against Escherichia coli and Staphylococcus aureus at a concentration of 100 μg/mL, while the antibacterial effect was unsatisfactory under dark conditions. Additionally, C 60 (OH) n -Ca nanoparticles promoted the proliferation of MC3T3-E1 cells, demonstrating desirable biological safety. The C 60 (OH) n -Ca nanoparticles synthesized in this article achieved a balance between antibacterial and cytocompatibility, thus possess potential applications in biomedical fields.
研究领域:
生物医学
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材料科学
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DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
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2018
·Vol. 24
·期 62
·16609-16619
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IF 5.16
Shengju Zhou,Linwen Zhang,Yongqiang Feng,Hongguang Li,Mengjun Chen,Wei Pan,Jingcheng Hao
Graphical Coming together : Highly pure fullerenols have been prepared by dialysis and show photoluminescence in water that is sensitive to the presence of Cu 2+ ions. When mixed with a cationic surfactant, a transition between the aggregates was induced from highly ordered vesicles to honeycomb-structured crystals (see picture; c s =concentration of the surfactant). Fullerenols have been known for decades; however, the photoluminescent (PL) properties and applications in supramolecular chemistry of these molecules have not been well addressed. Herein, a strategy has been developed to purify the as-prepared fullerenols, and the photoluminescence and capabilities of these molecules to form supramolecular self-assemblies were systematically studied. It was found that fullerenols show wavelength-dependent emission with an absolute fluorescent quantum yield of approximately 3.5 %. The PL characteristics are reminiscent of carbon dots, especially those obtained by using the top–down method. The studied fullerenols can be used to detect Cu 2+ ions with a limit of detection down to 3.50 μ m . In addition, the amphiphilicity of the fullerenols can be readily tuned by ionic complexation with cationic surfactants, such as 1-tetradecyl-3-methylimidazoliumbromide (C 14 mimB) and tetradecyltrimethylammonium bromide (TTAB). On increasing the concentration of the surfactant, the transition of aggregates was induced from highly ordered vesicles to honeycomb-structured crystals and finally to giant micelles. After the formation of supramolecular self-assemblies, enhancement of photoluminescence was observed, which can be ascribed to the suppression of intramolecular vibrations and motion combined with the loosely packed self-assembly array. This study provides a facile way to generate PL nanoarchitectures, which may find applications in fluorescent sensing, drug delivery, and optoelectronics.
研究领域:
材料科学
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化学
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DOI
PubMed
高影响因子
Small Methods
·
2025
·
IF 9.1
Jinrui Li,Huiyan Xie,Di Sun,Hongguang Li,Xia Xin
Exploring potential third-order nonlinear optical (NLO) materials attracts ever-increasing attention. Given that the atomically precise and rich adjustable structural features of silver nanoclusters (Ag NCs), as well as the unique π-electron conjugated system of carbon-based nanomaterials, a supramolecular co-assembly amplification strategy to enhance the luminescent intensity and NLO performance of the hybrids of the two components, are constructed and the relationship between structures and optical properties are investigated. By combining water soluble Ag NCs [(NH 4 ) 6 [Ag 6 (mna) 6 ] (H 2 mna = 2-mercaptonicotinic acid, abbreviated to Ag 6 ─NCs hereafter) containing uncoordinated carboxyl groups with water-soluble fullerene derivatives modified with multiple hydroxyl groups (fullerenols, C 60 ─OH), the π-electron delocalization is expanded owing to non-covalent hydrogen bonding effect between Ag6─NCs and C 60 ─OH, which provides a feasible basis for realizing the NLO response. Then, the co-assemblies are doped into a PMMA matrix to prepare composite film and its NLO properties are evaluated by Z-scan technique. Remarkably, the effective nonlinear absorption coefficients β is of two orders magnitude higher than those of the Ag 6 ─NCs assemblies at the absence of C 60 ─OH. This work showcases a new approach for amplifying NLO responses which greatly facilitates the development of integrated photonic devices.
研究领域:
材料科学
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光学
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DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2019
·
IF 0
Dan Xie,Ya-Nan Chang,Gengmei Xing,Lina Zhao,Min Li,Yuliang Zhao
Fullerenol has been demonstrated to be a potential anticancer nanodrug thanks to its excellent antioxidant properties. In this work, surface-enhanced Raman scattering (SERS)-based frequency shift method is employed to explore the interaction between fullerenol and the key digestive proteases (pepsin and trypsin) in the gastrointestinal tract. A dynamic adsorption process of fullerenol on pepsin/trypsin has been monitored by our detection platform. The binding sites of fullerenol to trypsin and pepsin are evidenced by introducing their inhibitors to the detection system, and the binding configurations/modes of fullerenol to pepsin and trypsin are precisely determined through computational simulations. The permeability of fullerenol through intestinal epithelial cells monolayer is also investigated and the results show that it can reach 6% in 6 h and 13% in 48 h, indicating its high oral bioavailability in human body. This work demonstrates the activity of fullerenol in the gastrointestinal tract and provides potential guidance for appropriate oral medicine design with the aid of a molecular docking method.
研究领域:
药理学
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生物化学
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纳米医学
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DOI
DYES AND PIGMENTS
·
2025
·
IF 4.2
Haonan Xu,Yuping Han,Danying Zhao,Zheng Liu,Guibin Wang,Yunhe Zhang
In this study, we designed and synthesized a novel hyperbranched structure of phthalocyanine compound, which was axially linked with fullerenol to form a D-A (donor and acceptor) type compound HLaPc-C 60 (OH) n . In this configuration, phthalocyanine acts as the electron donor, while fullerenol as the acceptor. The formation of hyperbranched structures and the introduction of fullerenol expanded the π-conjugation system and weakened the aggregation phenomenon. The unique three-dimensional hyperbranched structure with abundance of D-A enhances the photoinduced intramolecular electron transfer (PET) and energy transfer (ET) processes. This improvement contributes to the compound's excellent nonlinear optical properties. In addition, we prepared a PPSU-based composite film (HLaPc-C 60 (OH) n /PPSU) in which the interaction between the similar sulfonyl structures and HLaPc enhances its dispersion. The values of large third-order nonlinear susceptibility (Im[χ (3) ]) of 2.20 × 10 −8 esu and a low limit threshold (Ilim) of 0.91 J/cm 2 demonstrate that the composite film material has considerable potential applications as optical limiters.
研究领域:
材料化学
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光物理化学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Yunqi Wang,Peng Zhou,Qingguo Wang,Xiaowei Huo,Xue Huang,Qian Ye,Hao Xu,Guanyu Zhou,Yuhang Liu,Jing Zhang
Hydroxylated fullerene, Fullerol, has applied in many areas because of its high electron affinity and antioxidant potential, while it was few used in wastewater treatment. In this study, the mechanism involved that Fullerol accelerated the regeneration of Fe(II) in the Fe(III)/H 2 O 2 system under dark condition, which was investigated with chloramphenicol (CAP) as a model pollutant. In Fullerol/Fe(III)/H 2 O 2 system, CAP removal was completely achieved at 60 min via a non-photochemical pathway. In addition, the quenching experiments and electron spin resonance (ESR) results indicated that hydroxyl radical ( OH) was the main reactive oxygen species (ROSs). The Fullerol/Fe(III)/H 2 O 2 system had a rate constant of CAP degradation about 5.0 × 10 −2 min −1 , which was about 22 times higher than it in Fe(III)/H 2 O 2 Fenton-like system (2.3 × 10 −3 min −1 ). Fe(II) was ascertained the effective component that was responsible for H 2 O 2 activation to produce OH, which derived from 1) the first pathway: Fullerol complexed with Fe(III) and the redox potential of the complex is much lower than Fe(III)/Fe(II); 2) the second pathway: Fullerol acted as an electron donor to reduce Fe(III) into Fe(II). Moreover, CAP degradation intermediates were identified and its degradation pathways were proposed. This study suggested a new approach for improving the Fe(III)/H 2 O 2 system by accelerating Fe(III)/Fe(II) cycles with Fullerol.
研究领域:
环境科学
·
废水处理
·
化学工程
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产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2019
·
IF 14.229
Peng Zhou,Jing Zhang,Zhaokun Xiong,Yang Liu,Xiaowei Huo,Xin Cheng,Wenshu Li,Feng Cheng,Yongli Zhang
Hydroxylated C 60 Fullerene (Fullerol) induced photochemical production of reactive oxygen species (ROS, e.g. singlet oxygen ( 1 O 2 ) and hydroxyl radical ( OH)) is a slow process, and the role of its surface groups other than high water-solubility is still lack of investigation. In this study, we demonstrate Fullerol can promote Fe(III)/Fe(II) cycles to degrade dimethyl phthalate (DMP) in Fe(III)/H 2 O 2 Fenton system as both an electron donor and an electron-transfer mediator. At acidic condition, the formation of carbonyl and carboxyl via pinacol rearrangement and subsequent keto-enol isomerization can produce cage-opened structure of Fullerol, resulting in the pH-dependent formation of Fe(III)-Fullerol complex. Two pathways of Fe(II) recovery via intramolecular electron transfer of Fe(III)-Fullerol complex to enhance Fe(III)/H 2 O 2 Fenton oxidation were proposed: 1) Fullerol direct reduces Fe(III) into Fe(II) as an electron donor in dark, and it was strongly enhanced under visible light irradiation; 2) Fullerol mediates electron transfer from electron donor (e.g. hydrogen peroxide and organics) to Fe(III) in visible light condition. Furthermore, DMP degradation pathway was proposed based on variation of main intermediates.
研究领域:
环境科学
·
化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Peng Zhou,Xiaowei Huo,Jing Zhang,Yang Liu,Feng Cheng,Xin Cheng,Yunqi Wang,Yongli Zhang
Hydroxylated fullerene (fullerenol) has gained increasing attention in the field of water environment remediation based on its intrinsic properties of photosensitivity and high water solubility. In this work, C 60 fullerenol was added to the Fe(III)/PMS process to enhance diethyl phthalate (DEP) degradation both with and without visible light. The DEP removal ratio was increased from 2.2% to 58.8% with the addition of fullerenol at 60 min in dark condition, and the removal of DEP was completely achieved within 50 min under visible light irradiation. Mechanism investigation shows that carboxyl and carbonyl were formed via keto-enol isomerization at acid condition, resulting in the formation of open-cage fullerenol. The open-cage fullerenol can act as complex agent and electron donor to enhance oxidizing capability of the Fe(III)/PMS process by promoting Fe(III)/Fe(II) cycles. Competitive radical capture tests by electron spin resonance (ESR) and radical quenching analysis demonstrate that hydroxyl radical and sulfate radical were primarily responsible for DEP removal, but not superoxide radical and singlet oxygen. The DEP removal in the F/Fe(III)/PMS process is more efficiency at initial pH ranging from 2.1 to 4.9. Moreover, the DEP degradation route was proposed involving the generation of various intermediary products. This study provides a novel method for the application of fullerene derivatives on environment remediation by combining with metal catalyzed advanced oxidation processes.
研究领域:
环境科学
·
化学
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产品关联
DOI
高影响因子
Nanomaterials
·
2021
·Vol. 12
·期 1
·139
·
IF 5.076
Shuqing Chen,Qi Ding,Yan Gu,Xin Quan,Ying Ma,Yulong Jia,Hongmei Xie,Jinzhu Tang
The tribological performances of fullerenol and nanodiamonds (NDs) as additives in water-based lubricants for amorphous carbon (a-C) coatings are investigated to avoid disadvantage factors, such as chemical reactions and deformation of particles. The effects of size and additive amount on tribological properties of nanoparticles are studied by rigid nanoparticles within the dot size range. The results show that owing to its small particle size (1–2 nm), fullerenol cannot prevent direct contact of the friction pair at low concentration conditions. Only when the quantity of fullerenol increased to support the asperity contact loads in sufficient concentration did nano-bearings perform well in anti-friction and anti-wear effects. Unlike fullerenol, nanodiamond particles with a diameter of about 5–10 nm show friction-reducing effect based on the nano-bearing effects at ultra-low concentration (0.01 wt.%), whereas particles at higher concentration block the rolling movement, hence increasing the coefficient of friction (COF) and wear. As a result of the effect of difference in size, fullerenol provides a better overall lubrication, but it is hard to reach a friction coefficient as low as NDs even under the optimal conditions.Keywords:fullerenol;nanodiamonds;water-based lubricant additive;a-C;nano-bearing effects
研究领域:
材料科学
·
摩擦学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）