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JOURNAL OF APPLIED POLYMER SCIENCE · 2025 · IF 2.8

Polyethylene/Hydrophobic Calcium Lignosulfonate Composite Film for Anti-Photooxidation of Fuji Apples

Song Zhang,Zhang Chen,Yanfeng Gao

Excessive ultraviolet (UV) radiation exposure induces photooxidative stress in plants, leading to severe cell damage. Current packaging films often rely on nonrenewable, petroleum-based UV absorbers, which may pose potential health risks. Calcium lignosulfonate, a natural byproduct derived from the pulp and paper industry, has attracted significant attention as a sustainable alternative to petroleum-based UV absorbers. In this study, calcium lignosulfonate was hydrophobically modified to enhance its compatibility with nonpolar low-density polyethylene (LDPE), and a UV-blocking composite film was subsequently fabricated. The hydrophobic modification significantly increased the water contact angle of calcium lignosulfonate from 5° to 101°. Incorporating the modified calcium lignosulfonate into LDPE improved both the melting point and crystallinity of the polymer. The resulting composite film demonstrated excellent UV-blocking performance, achieving a UV-A blocking rate of 95% and a UV-B blocking rate of 99%, and effectively mitigated photooxidative browning in “Fuji” apples. This film exhibits great potential for anti-photooxidation packaging applications while simultaneously reducing dependence on petroleum-based resources.

研究领域: 食品科学 · 材料科学
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JOURNAL OF FOOD BIOCHEMISTRY · 2021 ·Vol. 45 ·期 12 ·e13995 · IF 2.72

Effect of ascorbic acid on tyrosinase and its anti-browning activity in fresh-cut Fuji apple

Yi-Ting Wen,Yu-Qin Liang,Wei-Ming Chai,Qi-Ming Wei,Zi-Yi Yu,Lin-Jun Wang

Tyrosinase (polyphenol oxidase) is the key enzyme of enzymatic browning in fruits and vegetables. In this research, the impact of ascorbic acid on tyrosinase and its anti-browning effect on fresh-cut Fuji apple were investigated. Ascorbic acid had a dual effect on tyrosinase with a half inhibitory concentration (IC 50 ) of 13.40 ± 0.05 µM. Fluorescence assay demonstrated that ascorbic acid interacted with tyrosinase in a dynamic contaction caused by Förster’s resonance energy transfer (FRET) and induced a conformational change of the enzyme. Thermodynamic analysis, copper interaction, and molecular docking further confirmed that ascorbic acid could chelate the copper ions located in active center and interact with amino acid residues of tyrosinase via hydrophobic interaction. In addition, ascorbic acid prevented the browning of fresh-cut apples by increasing APX activity and inhibiting PPO and POD activities which reduce the oxidation of total phenolics and flavonoids. Practical applications The present study demonstrated that ascorbic acid had a strong inhibitory activity against tyrosinase (IC 50 = 13.40 ± 0.05 µM) and anti-browning activity against fresh-cut Fuji apple. It could delay the browning degree of apple juice, increase APX activity, inhibit PPO and POD activities, and reduce the oxidation of total phenolics and flavonoids. These findings provided a basis for the feasible application of ascorbic acid on the preservation of fruits.

研究领域: 食品科学 · 植物生理学
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Food Bioscience · 2022 · IF 5.318

Effects of secondary fermentation of four in-bottle Saccharomyces cerevisiae strains on sparkling cider sensory quality

Wenting Li,Yuning Zhao,Chen Liu,Shuai Li,Tianli Yue,Yahong Yuan

In this study, the first fermentation of Fuji apple juice was carried out using Saccharomyces cerevisiae WLS21 and continued a second fermentation using S. cerevisiae HYJ1, HYJ2, PE2, and Y41 in sealed bottles after adjusting the sugar level. The physicochemical indexes, aromas, and sensory characteristics of four sparkling ciders were analyzed. The results revealed that HYJ2 produced the highest amount of ethanol and Y41 had the highest pressure in the bottle at the end of fermentation. Y41 produced the highest citric and succinic acids and HYJ1 produced higher fumaric acid and total volatiles. HYJ1 cider had an obvious herbaceous odor, and Y41 cider had a fruity character. Sixty-seven volatiles were detected from four sparkling ciders (55 fermentative and 12 non-fermentative aromas). Esters (EEFAs and HAAs) were the main volatile aroma family produced during secondary fermentation, and terpenes contributed the most to fruity odor. Based on univariate (ANOVA) and multivariate analysis of variance (PLS-DA) screening of four cider significant variables, 13 volatiles with variable predictive importance (VIP)≥1 were identified. PLSR analysis revealed that 2-methylbutyl acetate, ethyl heptanoate, 2-methyl-1-butanol, nonanal, 2-nonanone, β-damascenone, and linalool were potential volatile markers for OAV>1.

CAS / MDL: 589-98-0
研究领域: 食品科学 · 饮料技术 · 微生物学 · 农学
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SCIENTIA HORTICULTURAE · 2017 · IF 1.624

Rhizospheric application with 5-aminolevulinic acid improves coloration and quality in ‘Fuji’ apples

Jie Zheng,Yu-yan An,Xin-xin Feng,Liang-ju Wang

5-Aminolevulinic acid (5-ALA), an eco-friendly plant growth regulator, has been increasingly applied to crop and fruit production. In the actual production, many fruits are often covered by plastic bags which will not be removed until the fruits are harvested. Therefore, although 5-ALA shows promotive effect on fruit quality, fruit spray cannot be implemented in the orchards using plastic bags. In this study, rhizospheric application of 5-ALA increased the endogenous 5-ALA content in the leaves and fruits, suggesting that 5-ALA is transported from roots to aboveground. Using a multiple plant efficiency analyzer (M-PEA), we also found that rhizospheric application of 5-ALA significantly improved PS II and PS I photosynthetic activities of apple leaves. It means that 5-ALA-treated apple trees might accumulate more assimilates for fruits to improve their quality. Meanwhile, rhizospheric application of 5-ALA obviously increased fruit weight, the content of flavonoids, total soluble solids and ascorbic acid, as well as activities of antioxidant enzymes. Most importantly, rhizospheric irrigation of 5-ALA also significantly improved fruit coloration. Using calli induced from the flesh of ‘Fuji’ fruits, we found that 5-ALA up-regulated the expressions of many genes involved in flavonoid anabolism. These results suggest that rhizospheric application of 5-ALA solution is effective to improve external and interior qualities of apple fruits although 5-ALA was not directly applied to fruit surface. Taken together, we conclude that rhizospheric application of 5-ALA can be transported to the leaves and fruits of apple trees, where it enhances leaf photosynthetic capacity and improves fruit coloration and interior qualities simultaneously. This finding provides an effective and handy method to improve apple quality in modern orchards.

研究领域: 园艺学 · 植物生理学 · 农学
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