JOURNAL OF NANOPARTICLE RESEARCH
·
2021
·Vol. 23
·期 5
·1-14
·
IF 2.253
Zhu Li,Song Zhimei,Feng Sijia,Xu Hongmei,Chen Shiyu,Feng Runliang
In the study, biotin-modified oligochitosan-F127 conjugate (F127-CS-BIO) was synthesized as a carrier to prepare honokiol-loaded micelles via dialysis method. The encapsulation efficiency and drug loading were 70.79 ± 2.70% and 9.70 ± 0.57%, respectively. The mean particle size and zeta potentials were 210.9 ± 4.65 nm and 12.9 ± 7.22 mV, respectively. In vitro release studies showed that the release of honokiol from the micelles was slow and steady. MTT evaluation and cellular uptake results demonstrated that F127-CS-BIO was more easily absorbed by tumor cells and resulted in low IC 50 value of honokiol due to biotin’s targeting guidance when compared with biotin-unmodified F127-CS. In conclusion, F127-CS-BIO polymer was expected as an effective nanocarrier to encapsulate chemotherapeutic drugs. Graphical abstract
研究领域:
药剂学
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纳米医学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Tong Sun,Xi Guo,Rui Zhong,Lu Ma,Hao Li,Zhipeng Gu,Junwen Guan,Hong Tan,Chao You,Meng Tian
Oligochitosan (OCHI) is known to have some specific biological activities. However, its interactions with blood components and related correlation with molecular structures remains to be clarified due to its growing use in biomedical areas. Herein, a series of OCHI were prepared by hydrogen peroxide induced degradation combined fractionation in ethanol solutions and their molecular structures were characterized by GPC, FTIR, 1 H and 13 C NMR, and then the interactions of the prepared OCHI with blood components, including red blood cells (hemolysis, deformability, and aggregation), coagulation system, complement (C3a, and C5a activation), and platelet (activation, and aggregation), were investigated. For red blood cells, OCHI has a quite low risk of hemolysis in a dose- and MW-dependent manner and the deformability and aggregation were observed in its high MW fraction. The coagulation tests revealed that OCHI is capable of a mild anticoagulation through blocking the intrinsic pathway and the anticoagulation corresponding MW was identified. In terms of complement, OCHI could inhibit C3a in a dose-dependent manner and activate C5a with its high MW fraction. In addition, there is no significant effect of OCHI on platelet activation and aggregation. Based on above results, the interactions related mechanism was discussed and proposed.
研究领域:
生物医学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Hui Han,Liqin Tang,Yan Li,Yong Li,Ming Bi,Jun Wang,Fujun Wang,Lu Wang,Jifu Mao
Surgical wound closure is accomplished most frequently with sutures, optimally proceeding rapidly and without complication. However, surgical sutures can trigger foreign body reactions and incite abnormal collagen deposition. Sustained inflammation can result in abnormal wound healing with hypertrophic scar formation. Therefore, evolution of suture material to inhibit inflammation and scar formation is of great clinical significance. In the present study, commercial 3-0 PPDO [poly( p -dioxanone)] suture was used as the base material and modified by adding two layers: a drug-loaded layer and an electroactive layer. The former layer was curcumin (Cur) encapsulated by PLGA [poly (lactic- co -glycolic acid)] and the latter layer was composed of oligochitosan-gelatin/tannic acid/polypyrrole (OCS-GE/TA/PPy). The multifunctional sutures, named S@LC@CGTP, had desirable sustained-drug release properties in vitro where Cur could be released for 8 days due to the action of PLGA. The three-dimensional network structure of OCS-GE/TA ensured S@LC@CGTP against surface cracking and maintained electrical. Furthermore, using an in vivo experiment, S@LC@CGTP could attenuate inflammation and promote scar-free wound healing according to suppression of infiltrating inflammatory cells, down-regulation of TGF-β1 and collagen type I expression, and improved collagen arrangement. Cumulatively, we indicated that S@LC@CGTP suture material has great potential to facilitate optimal, nearly scarless healing of surgical incisions.
研究领域:
生物医学工程
·
材料科学
·
创伤修复
✓ 已验证
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2019
·
IF 3.42
Longfang Ren,Xudong Guo,Yongxia Zhao,Taotao Qiang
Phenolic acid grafed oligochitosan (PA- g -OCS and GA- g -OCS) have been synthesized through the reaction of protocatechuic acid and gallic acid with oligochitosan, which was catalyzed by laccase. Subsequently, the two modified waterborne polyurethanes (POCS-WPU and GOCS-WPU) were obtained using PA- g -OCS and GA- g -OCS as biobased crossing agents to react with polyurethane prepolymer, respectively. The structure of POCS-WPU and GOCS-WPU was characterized by Fourier transform infrared (FTIR) and Ultraviolet-Visible absorption (UV–vis) spectroscopy, and the properties of POCS-WPU and GOCS-WPU coatings were examined. The results elaborated that both of the films present fine water contact angle, pronounced mechanical properties (tensile strength, 23.26 and 29.39 MPa; elongation at break, 504 and 491%, respectively) and significant thermal stability. Moreover, the polymers exhibited favorable antioxidant activity measured from the DPPH and ABTS radical scavenging assays.
研究领域:
材料科学
·
高分子化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Pengchao Xie,Yan Peng,Liyan Qiu
To explore effective therapeutic strategy on nonalcoholic fatty liver disease (NAFLD), the amphiphilic oligochitosan derivative containing ursodeoxycholic acid (UDCA) was synthesized and named as UBC, which could self-assemble and encapsulate exenatide (Exe) to obtain Exe-UBC nano-vesicle. Exe-UBC could be uptaken by fatty-acid cultured cells and release UDCA and Exe responsive to the high esterase concentration. In vitro experiments demonstrated that Exe-UBC activated the expression level of SIRT1 with inhibited expression of PGC-1β and PPAR-γ and consequently exerted synergistic bioaction immediately on reducing lipidosis. After a month of Exe-UBC treated through intravenous injection, the body weight of high-fat diet feeding C57BL/6 mice recovered to ordinary level, and their lipid contents in the liver declined significantly. The recovery in hepatic function indexes like TG , AST , and ALT further revealed the superiority of Exe-UBC vesicles. These results suggested that the co-delivery of UDCA and Exe via Exe-UBC could be a potent platform for NAFLD treatment.
研究领域:
药学
·
生物技术
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2023
·
IF 9.7
Mengjie Kong,Yan Peng,Liyan Qiu
Non-alcoholic fatty liver disease (NAFLD) is currently a common chronic liver disease worldwide. By now, however, there isn't any FDA-approved specific drug for NAFLD treatment. It has been noticed that farnesoid X receptor (FXR), miR-34a and Sirtuin1 (SIRT1) is related to the occurrence and development of NAFLD. A oligochitosan-derivated nanovesicle (UBC) with esterase responsive degradability was designed to co-encapsulate FXR agonist (obeticholic acid, OCA) and miR-34a antagomir (anta-miR-34a) into the hydrophobic membrane and the center aqueous lumen of nanovesicles, respectively, by dialysis method. The action of UBC/OCA/anta-miR-34a loop on the regulation of lipid deposition via nanovesicles was evaluated on high-fat HepG2 cells and HFD-induced mice. The obtained dual drug-loaded nanovesicles UBC/OCA/anta-miR-34a could enhance the cellular uptake and intracellular release of OCA and anta-miR-34a, leading to the reduced lipid deposition in high-fat HepG2 cells. In NAFLD mice models, UBC/OCA/anta-miR-34a achieved the best curative effect on the recovery of body weight and hepatic function. Meanwhile, in vitro and vivo experiments validated that UBC/OCA/anta-miR-34a effectively activated the expression level of SIRT1 by enhancing the FXR/miR-34a/SIRT1 regulatory loop. This study provides a promising strategy for constructing oligochitosan-derivated nanovesicles to co-deliver OCA and anta-miR-34a for NAFLD treatment. Statement of significance This study proposed a strategy to construct oligochitosan-derivated nanovesicles to co-deliver obeticholic acid and miR-34a antagomir for NAFLD treatment. Based on the FXR/miR-34a/SIRT1 action loop, this nanovesicle effectively exerted a synergetic effect of OCA and anta-miR-34a to significantly regulate lipid deposition and recover liver function in NAFLD mice.
研究领域:
药学
·
分子生物学
·
肝病学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Xin Hu,Wen-xuan Hu,Hong-yu Lu,Song Liu,Sheng-qi Rao,Zhen-quan Yang,Xin-an Jiao
This study investigated the effects of enzyme action time (1–5 h) on the physicochemical properties, structure, and functionality of transglutaminase-catalyzed ovalbumin (OVA) in the presence of oligochitosan (OCS). Physically combining OVA and OCS led to a substantial increase in particle size and polydispersity index, rendering the mixed system unstable. With the increase of enzyme action time, the particle size of glycosylated and cross-linked OVA (GC-OVA) decreased first and then increased, reaching a minimum value of 411 nm at 4 h. Moreover, the decrease of free amino groups content at 4 h reflected the occurrence degree of glycosylation and cross-linking reaction, which was close to saturation. Diverse methods were used to analyze variations in the molecular structure of OVA, confirming changes in the primary, secondary, and tertiary structures, and the microstructure. The glycosylation grafting and cross-linking with the appropriate extent of the enzymatic action time is the primary reason for improving the foaming , emulsification properties, and DPPH activity of OVA. Moreover, GC-OVA had greater apparent viscosity, G′ and G″ than the other control systems (the untreated OVA and the physical mixture of OVA and OCS) and significantly affected the hardness and cohesion properties in the gel's texture (5.6 and 2.4-fold that of the untreated OVA, respectively). This study provides information for further understanding the effect of glycosylation grafting caused by enzyme cross-linking time on OVA. The study also demonstrates the possibility of obtaining new protein compositions to expand the applications of food proteins.
研究领域:
食品科学
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Shisuo Jing,Huayuan Chen,Ergang Liu,Meng Zhang,Feng Zeng,Huan Shen,Yuefei Fang,Bahtiyor Muhitdinov,Yongzhuo Huang
Inflammatory bowel disease (IBD) is a chronic, life quality-reducing disease with no cures available yet. To develop an effective medication suitable for long-term use is an urgent but unmet need. Quercetin (QT) is a natural dietary flavonoid with good safety and multifaceted pharmacological activities against inflammation. However, orally administrated quercetin yields unproductive outcomes for IBD treatment because of its poor solubility and extensive metabolism in the gastrointestinal tract . In this work, a colon-targeted QT delivery system (termed COS-CaP-QT) was developed, of which the pectin (PEC)/Ca 2+ microspheres were prepared and then crosslinked by oligochitosan (COS). The drug release profile of COS-CaP-QT was pH-dependent and colon microenvironment-responsive, and COS-CaP-QT showed preferential distribution in the colon. The mechanism study showed that QT triggered the Notch pathway to regulate the proliferation of T helper 2 (Th2) cells and group 3 innate lymphoid cells (ILC3s) and the inflammatory microenvironment was remodeled. The in vivo therapeutic results revealed that COS-CaP-QT could relieve the colitis symptoms and maintain the colon length and intestinal barrier integrity.
研究领域:
药剂学
·
药理学
·
胃肠病学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）