ENVIRONMENTAL POLLUTION
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2025
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IF 7.3
Bowen Duan,Senchao Lai,Qiaoze Zhu,Zhiwei Pan,Yingyi Zhang
Proteins in aerosols can undergo multiphase reactions when exposed to air pollutants like ozone (O 3 ) and nitrogen dioxide (NO 2 ), posing adverse impacts on the environment and human health. The influence of radiation on the reactions has not been thoroughly studied and the reaction mechanism remains unclear. We coated bovine serum albumin exposed (BSA) in a flow tube and exposed it to different combinations of O 3 and NO 2 under dark and ultraviolet (UV) radiation conditions. The results showed that O 3 could significantly promote the degradation, nitration and oligomerization of BSA, indicating that O 3 is the primary driver of the multiphase reactions. We found that UV enhanced protein degradation after exposure to NO 2 and O 3 . Meanwhile, UV also enhanced the nitration and oligomerization of protein by NO 2 . When exposed to NO 2 and O 3 , the effects of nitration and oligomerization on tyrosine residue of BSA was also investigated. Nitrated tyrosine (NTyr) levels were always higher than that of cross-linked tyrosine (DiTyr), highlighting the dominance of nitration in modification reactions. However, the negative influence of UV radiation on protein nitration and oligomerization of BSA was observed in our exposure experiment. We propose that UV radiation induced degradation may be an important factor to suppress nitration and oligomerization via the breakage of nitrated and oligomerized protein products. This work provides the dataset and knowledge by laboratory exposure to emphasize the possible role of radiation in the multiphase reactions of proteins in the atmosphere, which will help to gain a better understanding of reaction mechanisms.
研究领域:
环境科学
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生物化学
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DOI
PubMed
高影响因子
EMBO REPORTS
·
2022
·Vol. 23
·期 11
·e54569
·
IF 9.071
Yueke Lin,Xiaoting Lv,Caiyu Sun,Yanlin Sun,Min Yang,Dapeng Ma,Weiqiang Jing,Yunxue Zhao,Yeping Cheng,Haocheng Xuan,Lihui Han
Tripartite motif protein (TRIM) 50 is a new member of the tripartite motif family, and its biological function and the molecular mechanism it is involved in remain largely unknown. The NOD-like receptor family protein (NLRP)3 inflammasome is actively involved in a wide array of biological processes while mechanisms of its regulation remain to be fully clarified. Here, we demonstrate the role of TRIM50 in NLRP3 inflammasome activation. In contrast to the conventional E3 ligase functions of TRIM proteins, TRIM50 mediates direct oligomerization of NLRP3, thereby suppressing its ubiquitination and promoting inflammasome activation. Mechanistically, TRIM50 directly interacts with NLRP3 through its RING domain and induces NLRP3 oligomerization via its coiled-coil domain. Finally, we show that TRIM50 promotes NLRP3 inflammasome-mediated diseases in mice. We thus reveal a novel regulatory mechanism of NLRP3 via TRIM50 and suggest that modulating TRIM50 might represent a therapeutic strategy for NLRP3-dependent pathologies.
研究领域:
免疫学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
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2022
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IF 4.117
Tan Qin,Yu Wang,Jing Nie,Lushan Yu,Su Zeng
Background Sodium taurocholate co -transportering polypeptide (NTCP, SLC10A1 ) is a vital bile acid transporter and the functional receptor of hepatitis B and D virus. The oligomerization of NTCP is important for the structural study of its interaction with HBV preS1 peptide. Methods Recombinant NTCPs were expressed in Sf9 host cell using baculoviruses. Function of recombinant NTCP was verified by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method. A quantitative fluorescence resonance energy transfer (FRET) method was established to analyze the interaction between NTCP wild type (WT) and mutants. Co-immunoprecipitation (Co-IP) was used to test the interaction between NTCP variants. Results Sub-cellular location of recombinant NTCPs varies with the modification of NTCP. Bands of monomer, dimer and oligomers were shown in gel analysis of NTCP. Significant FRET was observed between cyan florescence protein (CFP) tagged NTCP and yellow florescence protein (YFP) tagged NTCP. FRET efficiency between CFP- and YFP-NTCP S267F mutants was lower than WT. Co-IP results showed that S267F interacts with WT NTCP when co-expressed in cell. Conclusion Dimer is the predominant form of NTCP expressed in Sf9 when solubilized with detergent. FRET and Co-IP analysis support that NTCP forms oligomers in Sf9 cell. General significance Our results showed that NTCP formed oligomers in Sf9 cell. Meanwhile the FRET analysis of NTCP variants further elucidated the molecular mechanism of NTCP oligomerization.
研究领域:
病毒学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2018
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IF 5.089
Xiaojun Fu,Wusigale,Hao Cheng,Zheng Fang,Li Liang
Proteins have been widely used to prepare effective carriers for the protection of bioactive components. There were rare studies on proteins' change during protection and its impact on the protection, albeit they are important for the choosing of suitable proteins used as carrier materials. Folic acid is a synthetic form of folate but decomposes under ultraviolet irradiation. In this study, improved protection of whey protein isolate (WPI) against the photodecomposition of folic acid was investigated by using fluorescence and HPLC. During the vitamin decomposition, WPI's change was characterized in term of composition, structure, interaction with the vitamin or its decomposition products. Folic acid decomposition photosensitized indirect oxidation of WPI, leading to unfolding, degradation in concurrence with oligomerization . The increase in the absorbance and antioxidant activity contributed to the protein improved protection for folic acid. WPI could thus be a good carrier material for the encapsulation and protection of folic acid.
研究领域:
食品科学
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营养学
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DOI
高影响因子
CHEMOSPHERE
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2021
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IF 7.086
Shunyao Li,Kai Sun
Humic constituents (HCs) are ubiquitous in the aquatic ecosystems , and contain various functional groups that seriously impact the conversion of 17 β -estradiol (17 β -E2) by fungal laccase. The purpose of this study was to explore the influencing mechanism of HCs on Trametes versicolor laccase-enabled 17 β -E2 oxidation and oligomerization. Herein, T. versicolor -secreted laccase could rapidly convert 99.2% of 17 β -E2 (rate constant = 3.7 × 10 −2 min −1 , half-life = 18.7 min) into multifarious oligomers at 25 °C and pH 5.0, by phenolic radical-caused C–C and/or C–O self-linking routes, whereas HCs with O -phenolic hydroxyl groups ( O -p-OH, i.e. , catechol, pyrogallol, gallic acid, and caffeic acid) dramatically suppressed 17 β -E2 oligomerization. Compared with HC-free, 17 β -E2 rate constants weakened 6.3–15.8 fold in the presence of HCs containing O -p-OH. It is largely because the O -p-OH was preferentially oxidized by T. versicolor laccase to create the electrophilic O -quinone monomers/oligomers. These unstable reactive O -quinone intermediates strongly reversed 17 β -E2 phenolic radicals to their monomeric molecules via two proton-transfer versus two electron-transfer channels, thus intercepting 17 β -E2 oxidation and oligomerization. These findings highlight new insights into the effect of HCs containing O -p-OH on T. versicolor laccase-started 17 β -E2 conversion, which is beneficial to re-understanding the fate and geochemical behavior of 17 β -E2 in water.
研究领域:
环境科学
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生物化学
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水生生态学
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酶学
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DOI
PubMed
高影响因子
GOLD BULLETIN
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2023
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IF 2.2
Hong Qing,Jin Xinchun,Zhou Chenheng,Shao Jiahui
Protein oligomerization contributes to Alzheimer’s disease development (AD). A nanoparticle that can speed up the oligomerization of proteins is generally considered harmful. Gold nanoparticles (AuNPs) have been reported to be making headway in biological platforms, but they may also have the capacity to stimulate protein oligomerization. Our goal herein was to investigate the neurotoxicity and oligomerization of amyloid-β-1-42 (Aβ 1-42 ) in the presence of AuNPs. The precipitation approach was used to create AuNPs, which were then analyzed using transmission electron microscopy (TEM), ThT, Congo red, and CD spectroscopy. The results demonstrated that the 50-nm-sized fabricated AuNPs guided acceleration in Aβ 1-42 . In addition, cytotoxicity studies on PC 12 cells showed that Aβ 1-42 with AuNPs were less toxic than untreated oligomers Aβ 1-42 in terms of inducing cell death, oxidative apoptosis, stress, and membrane leakage. In conclusion, our investigation sheds light on how AuNPs stimulate the development of cytotoxic oligomers by binding to proteins in Alzheimer’s disease.
研究领域:
生物医学
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纳米技术
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神经科学
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DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
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2018
·Vol. 36
·期 11
·1207-1216
·
IF 2.016
Zhang Wei,Jiang Bin-Bo,Ye Jian,Liao Zu-Wei,Huang Zheng-Liang,Wang Jing-Dai,Yang Yong-Rong
In this study, a series of aryloxy-aluminoxanes originated directly from the hydrolysis of reaction products of AlMe 3 and phenols were synthesized, which could serve as effective polymer-retarding activators for the iron-catalyzed ethylene oligomerization. The molar ratios of [PhOH]/[AlMe 3 ] and [H 2 O]/[Al] during the preparation were explored and their impacts on the oligomerization activity and product distribution were discussed. To obtain the effective activators with good polymer-retarding effect and relatively high activity, the optimized conditions were proposed to be [PhOH]/[AlMe 3 ] = 0.5 and [H 2 O]/[Al] = 0.7. Various aluminoxanes with different [―OH] sources confirmed the importance of using phenols in preparing the effective polymer-retarding activators. By utilizing these aryloxy-aluminoxanes, the mass fraction of polymers in the total products could be reduced to lower than 1.0 wt%, which is much lower than that of the MAO-activated systems (> 30 wt%). This is a potential benefit for the industrial application of the iron-catalyzed oligomerization process.
研究领域:
化学工程
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催化科学
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DOI
高影响因子
Journal of Translational Medicine
·
2024
·Vol. 22
·期 1
·1-16
·
IF 6.1
Xiong Yi,Chen Jiawen,Liang Wei,Li Kun,Huang Yingqi,Song Jingwen,Zhang Baoyu,Qiu Xiusheng,Qiu Dongbo,Zhang Qi,Qin Yunfei
Background Liver surgery during the perioperative period often leads to a significant complication known as hepatic ischemia-reperfusion (I/R) injury. Hepatic I/R injury is linked to the innate immune response. The cGAS-STING pathway triggers the activation of innate immune through the detection of DNA within cells. Nevertheless, the precise mechanism and significance of the cGAS-STING pathway in hepatic I/R injury are yet to be investigated. Methods Mouse model of hepatic I/R injury was used in the C57BL/6 WT mice and the STING knockout (STING-KO) mice. In addition, purified primary hepatocytes were used to construct oxygen-glucose deprivation reperfusion (OGD-Rep) treatment models. Results Our research revealed a notable increase in mRNA and protein levels of cGAS and STING in liver during I/R injury. Interestingly, the lack of STING exhibited a safeguarding impact on hepatic I/R injury by suppressing the elevation of liver enzymes, liver cell death, and inflammation. Furthermore, pharmacological cGAS and STING inhibition recapitulated these phenomena. Macrophages play a crucial role in the activation of the cGAS-STING pathway during hepatic I/R injury. The cGAS-STING pathway experiences a significant decrease in activity and hepatic I/R injury is greatly diminished following the elimination of macrophages. Significantly, we demonstrate that the activation of the cGAS-STING pathway is primarily caused by the liberation of mitochondrial DNA (mtDNA) rather than nuclear DNA (nDNA). Moreover, the safeguarding of the liver against I/R injury is also attributed to the hindrance of mtDNA release through the utilization of inhibitors targeting mPTP and VDAC oligomerization. Conclusions The results of our study suggest that the release of mtDNA plays a significant role in causing damage to liver by activating the cGAS-STING pathway during I/R injury. Furthermore, inhibiting the release of mtDNA can provide effective protection against hepatic I/R injury.
研究领域:
免疫学
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肝病学
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分子生物学
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DOI
PubMed
高影响因子
DEVELOPMENTAL CELL
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2022
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IF 13.417
Wen-Cheng Liu,Ru-Feng Song,Yi-Min Qiu,Si-Qiu Zheng,Ting-Ting Li,Yan Wu,Chun-Peng Song,Ying-Tang Lu,Hong-Mei Yuan
Summary H 2 O 2 affects the expression of genes that are involved in plant responses to diverse environmental stresses; however, the underlying mechanisms remain elusive. Here, we demonstrate that H 2 O 2 enhances plant freezing tolerance through its effect on a protein product of low expression of osmotically responsive genes2 ( LOS2 ). LOS2 is translated into a major product, cytosolic enolase2 (ENO2), and sometimes an alternative product, the transcription repressor c-Myc-binding protein (MBP-1). ENO2, but not MBP-1, promotes cold tolerance by binding the promoter of C-repeat/DRE binding factor1 ( CBF1 ), a central transcription factor in plant cold signaling, thus activating its expression. Overexpression of CBF1 restores freezing sensitivity of a LOS2 loss-of-function mutant. Furthermore, cold-induced H 2 O 2 increases nuclear import and transcriptional binding activity of ENO2 by sulfenylating cysteine 408 and thereby promotes its oligomerization . Collectively, our results illustrate how H 2 O 2 activates plant cold responses by sulfenylating ENO2 and promoting its oligomerization, leading to enhanced nuclear translocation and transcriptional activation of CBF1 .
研究领域:
植物生理学
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分子生物学
·
应激反应
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Qiuju Shi,Lei Gao,Wenjian Li,Jiaxin Wang,Zhang Shi,Yixin Li,Jiangyao Chen,Yuemeng Ji,Taicheng An
Uncertain chemical mechanisms leading to brown carbon (BrC) formation affect the drivers of the radiative effects of aerosols in current climate predictions. Herein, the aqueous-phase reactions of methylglyoxal (MG) and typical reduced nitrogen species (RNSs) are systematically investigated by using combined quantum chemical calculations and laboratory experiments. Imines and diimines are identified from the mixtures of methylamine (MA) and ammonia (AM) with MG, but not from dimethylamine (DA) with the MG mixture under acidic conditions, because deprotonation of DA cationic intermediates is hindered by the amino groups occupied by two methyl groups. It leads to N-heterocycle (NHC) formation in the MG + MA (MGM) and MG + AM (MGA) reaction systems but to N-containing chain oligomer formation in the MG + DA (MGD) reaction system. Distinct product formation is attributed to electrostatic attraction and steric hindrance, which are regulated by the methyl groups of RNSs. The light absorption and adverse effects of NHCs are also strongly related to the methyl groups of RNSs. Our finding reveals that BrC formation is mainly contributed from MG reaction with RNSs with less methyl groups, which have more abundant and broad sources in the urban environments.
研究领域:
大气化学
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环境科学
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量子化学
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产品关联
DOI
PubMed
高影响因子
FUEL
·
2021
·
IF 6.609
Jian Chen,Hongyan Wang,Lili Deng,Hui Wang,Zengxi Li
Butene oligomerization followed by hydrogenation is regarded as a promising way to produce alkylate oil, and the challenge of this process is the oriented conversion of butene to octene, which closely relates to the alkylate quality. Ionic liquids (ILs) are a kind of promising catalyst for olefin oligomerization. In this study, a series of functional metal-free acidic ILs (11 in total) were synthesized, characterized, and used for the oligomerization of iso butene. It was found that among the tested neat ILs, sulfobutyltriethylammonium triflate ([TENBs][CF 3 SO 3 ]) exhibited the best performance. This IL catalyst could be reused for 25 times, without obvious loss in catalytic activity. To improve catalytic activity of the neat IL, composite ILs formed by mixing [TENBs][CF 3 SO 3 ] with a second functional IL were developed. In the best composite system with tunable acidity, iso butene conversion could increase by ~88% compared with the neat IL, and meantime the dimer selectivity could increase to 79%. The conversion could increase from 25% to over 80% in the best composite system with enhanced mass transfer capability. Spectroscopic characterization and Gaussian simulation revealed the different catalytic behaviors of these ILs, which demonstrated that ILs with lower bond dissociation enthalpy (< 521 KJ·mol −1 ) and proper binding energy with iso butene (−27 to −20 KJ·mol −1 ) could efficiently catalyze iso butene oligomerization, the acidic active sites were from the sulfonic acid group in the cation and the acidic anion, and that deactivation of the IL catalyst after being used for 29 times could be attributed to the loss of the acid sites in both the cation and anion.
研究领域:
化学工程
·
催化材料
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xuan Zhao,Qingzhi Han,Na Na,Jin Ouyang
Cell membrane surface receptor proteins play an important role in cellular biological processes. There are numerous methods to detect receptors, yet developing an artificially controlled and specific detection and treatment strategy remains a challenge. Herein, we develop such a strategy based on upconversion nanoparticles (UCNPs) loaded DNA probes that enable two-color ratiometric imaging excitated by a 980 nm laser. The light response controllable signal opening strategy avoids waste during probe transportation and improves sensitivity. Thereby the number of receptors on individual DU145 cell membranes is counted by single-molecule detection. Due to the different expression of specific receptor proteins, the number of single fluorescent dots counted can be used as a basis for distinguishing DU145 from other cells. This work is highly controllable to increase sensitivity, providing a platform for cancer diagnosis and treatment.
研究领域:
生物化学
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分子生物学
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纳米医学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
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