MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Mai Luo,Jinchao Wei,Yunyang Zhao,Yuzhou Sun,Hengxing Liang,Shuangpeng Wang,Peng Li
Methyl-paraoxon (MP) is a representative organophosphorus pesticide (OP), which is a huge threat to the safety of human beings. In this study, carbon dots doped with boron and nitrogen (B, N–CDs) were used as a fluorescent probe for MP detection. The prepared cerium oxide (CeO 2 ) decompose MP efficiently into p-nitrophenol (p-NP) and phosphate group in the alkaline condition. The dissociative p-NP could reduce the fluorescent intensity of B, N–CDs through the inner filter effect (IFE). The pH value, incubation time, the amount of B, N–CDs and solvent effect were investigated and optimized for detection system. Under the optimum conditions, the limit of the detection was calculated to be 24.7 ng mL −1 with simple procedures. Tap water, lake water, Semen Coicis and Panax quinquefolius L. samples were used to test the recoveries of the detection system. The recoveries ranged from 94.68% to 107.28%. Furthermore, the fluorescence laminate was developed by painting B, N–CDs on the polyamide laminate. The MP residue could be observed directly under the 365 nm lamp, and linearity for MP by using the fluorescent laminates was ranged from 0.015 to 1.5 mM. The present work provides a rapid and dual-signal based method (fluorescent and visual) for routine detection of MP in real samples.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
DOI
高影响因子
CARBON
·
2023
·
IF 10.9
Bo Liu,Meijie Cai,Xiangling Feng,Siyu Lu,Song Lin,Feng Tian
Serial nanocomposites consisting of blue fluorescent carbon dots (B-CDs) and MgO nanoparticles were facilely prepared. Then on-site detection and degradation of ethyl-paraoxon were achieved and evaluated. It was revealed that ethyl-paraoxon was firstly decomposed into p-nitrophenol by MgO. Then, p-nitrophenol could significantly quench the fluorescence of decorated B-CDs due to an inner filter effect (IFE). The 0.05B-CDs@MgO showed excellent comprehensive performance among the serial xB-CDs@MgO samples due to its relatively high degradation ability towards ethyl-paraoxon and potent initial fluorescence emission intensity at an excitation wavelength of 360 nm between pH 4 and 10. Finally, based on the IFE, on-site paper sensors via color recognition app on a smartphone were explored compared with a conventional fluorescence spectrometer. Both the quantitative fluorescence intensities and G/B values showed the multi-piecewise linear correlations to ethyl-paraoxon concentration up to 3000 μg/mL in river water with a limit of detection to 0.06 μg/mL and 3.48 μg/mL as well as high recovery rates between 99%-102% and 95%–116%, respectively. The in vitro cytotoxicity and phytotoxicity assessment of the 0.05B-CDs@MgO revealed dose-dependent effects. Such portable and integrated fluorescent B-CDs@MgO nanocomposites on paper arrays show excellent potential in shortening the detection time and reducing the detection cost as a new strategy for the on-site quantitative detection and degradation of ethyl-paraoxon in actual river water.
研究领域:
环境科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 47
·5727-5734
·
IF 2.896
Mengyao Li,Lulu Guo,Lili Chen,Chunhua Lin,Li Wang
Methyl paraoxon (MP) has attracted more and more attention in recent years because of its severe neurotoxicity and respiratory toxicity. Therefore, it is very urgent to develop new and sensitive MP detection methods for health protection and public safety. Covalent organic frameworks (COFs) are widely used in fluorescence detection because they can effectively transmit and amplify probe signals with multiple identical binding sites within an extended framework. Here, COFML-DHTA nanosheet material was synthesized by the solvothermal reaction of melem (ML) and 2,5-dihydroxyterephthalaldehyde (DHTA). The resulting COFML-DHTA exhibits remarkable luminescence quenching toward MP due to the relationship of competitive absorption and Förster resonance energy transfer between MP and COFML-DHTA. COFML-DHTA can be used for sensitive and selective detection of MP in a wide concentration range of 0.57 ng mL−1 to 30 μg mL−1 with a detection limit of 0.19 ng mL−1. The material has good chemical stability, excellent selectivity, good reusability and hydrophilicity, which provide more possibilities for COFs in the detection of pesticides.
研究领域:
材料科学
·
环境科学
·
毒理学
✓ 已验证
DOI
高影响因子
Journal of Pharmaceutical Analysis
·
2020
·
IF 2.673
Yuzhou Sun,Jinchao Wei,Jian Zou,Zehua Cheng,Zhongming Huang,Liqiang Gu,Zhangfeng Zhong,Shengliang Li,Yitao Wang,Peng Li
A new electrochemical sensor for organophosphate pesticide (methyl-paraoxon) detection based on bifunctional cerium oxide (CeO 2 ) nanozyme is here reported for the first time. Methyl-paraoxon was degraded into p -nitrophenol by using CeO 2 with phosphatase mimicking activity. The CeO 2 nanozyme-modified electrode was then synthesized to detect p -nitrophenol. Cyclic voltammetry was applied to investigate the electrochemical behavior of the modified electrode, which indicates that the signal enhancement effect may attribute to the coating of CeO 2 nanozyme. The current research also studied and discussed the main parameters affecting the analytical signal, including accumulation potential, accumulation time, and pH. Under the optimum conditions, the present method provided a wider linear range from 0.1 to 100 μmol/L for methyl-paraoxon with a detection limit of 0.06 μmol/L. To validate the proof of concept, the electrochemical sensor was then successfully applied for the determination of methyl-paraoxon in three herb samples, i.e., Coix lacryma-jobi, Adenophora stricta and Semen nelumbinis. Our findings may provide new insights into the application of bifunctional nanozyme in electrochemical detection of organophosphorus pesticide.
研究领域:
分析化学
·
环境科学
·
农药检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Xuan Zhang,Nan Hao,Shucheng Liu,Kai Wei,Changchang Ma,Jianming Pan,Sheng Feng
Methyl paraoxon (MP) is a highly toxic, efficient and broad-spectrum organophosphorus pesticide , which poses significant risks to ecological environment and human health. Many detection methods for MP are based on the enzyme catalytic or inhibition effect. But natural biological enzymes are relatively expensive and easy to be inactivated with a short service life . As a unique tool of nanotechnology with enzyme-like characteristics, nanozyme has attracted increasing concern. However, a large proportion of nanozymes lack the intrinsic specificity, becoming a main barrier of constraining their use in biochemical analysis. Here, we use a one-pot reverse microemulsion polymerization combine the gold nanoclusters (AuNCs) with molecularly imprinted polymers (MIPs), polydopamine (PDA) and hollow CeO 2 nanospheres to synthesize the bright red-orange fluorescence probe (CeO 2 @PDA@AuNCs-MIPs) with high phosphatase-like activity for selective detection of MP. The hollow structure possesses a specific surface area and porous matrix, which not only increases the exposure of active sites but also enhances the efficiency of mass and electron transport . Consequently, this structure significantly enhances the catalytic activity by reducing transport distances. The introduced MIPs provide the specific recognition sites for MP. And Ce (III) can excite aggregation induced emission of AuNCs and enhance the fluorescent signal. The absolute fluorescence quantum yield (FLQY) of CeO 2 @PDA@AuNCs-MIPs (1.41 %) was 12.8-fold higher than that of the GSH-AuNCs (0.11 %). With the presence of MP, Ce (IV)/Ce (III) species serve as the active sites to polarize and hydrolyze phosphate bonds to generate p -nitrophenol (p-NP), which can quench the fluorescent signal through the inner-filter effect. The as-prepared CeO 2 @PDA@AuNCs-MIPs nanozyme-based fluorescence method for MP detection displayed superior analytical performances with wide linearities range of 0.45–125 nM and the detection limit of 0.15 nM. Furthermore, the designed method offers satisfactory practical application ability. The developed method is simple and effective for the in-field detection.
研究领域:
环境科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2021
·Vol. 99
·期 1
·122-131
·
IF 2.326
Liu Shisheng,Wei Xiaohui,Lin Song,Guo Minjie
The preparation of MgO nanoparticles with relatively small sizes and highly active surface areas is still a great challenge nowadays. The principal objective of this work was to investigate and optimize the calcined conditions of MgO with smaller sizes and higher surface areas, which contributed to its better degradation ability towards paraoxon pollutants. Firstly, the aerogel Mg(OH) 2 nanosheets with an increased surface area of 349.0 m 2 /g was prepared via a combined sol–gel-hydrothermal method. Then the calcined MgO nanoparticles with different morphology, size dimensions, and specific surface areas were obtained at different calcination temperatures from 400 to 800 °C. The optimized average grain size of MgO obtained at 600 °C was achieved to 8.7 ± 3.2 nm with a relatively high surface area up to 231.4 m 2 /g. Meanwhile, such as-prepared MgO nanoparticles showed an excellent performance towards paraoxon degradation, and the maximum degradation amount of paraoxon-ethyl was up to 57.8 mg/g@90 min at room temperature. The kinetics of degradation was consistent with the pseudo-second-order model ( R 2 > 0.99). Such excellent degradation capacity was derived from more generations of superoxide radical (•O 2 − ) sites as revealed by using the nitro blue tetrazolium (NBT) as a probe. The results indicated that the MgO nanoparticles reported herein could be an effective candidate for the environmental remediation of organophosphorus toxin pollutants due to their facile scale-up, low cost, eco-friendly characteristic, and high removal efficiency.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Mai Luo,Ling Chen,Jinchao Wei,Xiping Cui,Zehua Cheng,Ting Wang,Incheng Chao,Yunyang Zhao,Hao Gao,Peng Li
Exogenous pollution of Chinese medicinal materials by pesticide residues and heavy metal ions has attracted great attention. Relying on the rapid development of nanotechnology and multidisciplinary fields, fluorescent techniques have been widely applied in contaminant detection and pollution monitoring due to their advantages of simple preparation, low cost, high throughput and others. Most importantly, synchronous detection of multi-targets has always been pursued as one of the major goals in the design of fluorescent probes. Herein, we firstly develop a simultaneous sensing method for methyl-paraoxon (MP) and Nickel ion (Ni, Ⅱ) by using carbon based fluorescent nanocomposite with ratiometric signal readout and nanozyme. Notably, the designed system showed excellent effectiveness even when the two pollutants co-exist. Under the optimum conditions, this method provides low limits of detection of 1.25 µM for methyl-paraoxon and 0.01 µM for Ni (Ⅱ). To further verify the reliability, recovery studies of these two analytes were performed on ginseng radix et rhizoma, nelumbinis semen, and water samples. In addition, smartphone-based visual analysis has been introduced to expand its applicability in point of care detection. This work not only expands the application of the dual-mode approach to pollutant detection, but also provides insights into the analysis of multiple pollutants in a single assay.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Molecular Medicine Reports
·
2016
·Vol. 15
·期 1
·387-395
·
IF 1.559
Bing Wang,Rui‑Ning Yang,Yue‑Rong Zhu,Ji‑Cheng Xing,Xiao‑Wei Lou,Yu‑Jie He,Qi‑Long Ding,Ming‑Yue Zhang,Hong Qiu
Xanthine oxidase (XOD) and paraoxonase 1 (PON1) are important enzymes in redox reactions in vivo, and are predominantly synthesized by the liver. The aim of the present study was to investigate the redox state in nonalcoholic fatty liver disease, and determine the association between the activities of XOD and PON1 and the severity of NAFLD. Sprague‑Dawley rats were randomly divided into control, model and α‑lipoic acid (high and low dose) groups. The rats in the NAFLD model were induced by feeding a high fat diet for 12 weeks and the in vitro cell model of hepatocyte steatosis was induced by treating L‑02 cells with oleic acid for 24 h. The body weight, liver function, lipid and oxidative stress indices, and histological features of the liver were examined in the rats. Compared with the control group, the rats in the NAFLD model group showed impaired liver function, lipid disorders and damage from oxidative stress. The serum activity of XOD increased significantly from the 4th week and was markedly higher, compared with that in the control group, reaching a peak in the 12th week. The activity of PON1 was negatively correlated with that of XOD. Compared with the control cells, the activity of XOD and levels of free‑fatty acids were significantly higher, and the activity of PON1 was significantly lower in the NAFLD L‑02 cell model. All the above indicators were significantly improved by treatment with the antioxidant, α‑lipoic acid. The activities of XOD and PON1 may be promising as markers in a noninvasive approach for detecting the severity of NAFLD clinically. α‑lipoic acid had protective effects on the NAFLD rats, and the potential mechanism may be associated with the inhibition of oxidative stress and lipid peroxidation.
研究领域:
肝病学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Qingzhen Tian,Jinjin Liu,Yuxuan Long,Hao Liang,Kechen Wu,Xi Chen,Qinqin Bai,Xiangheng Niu
Pesticide sensing crucially safeguards food safety and public health against environmental and health hazards. While oxidoreductase-type nanozymes (peroxidase and oxidase) have been widely used in optical pesticide detection, their susceptibility to redox interference as well as poor target specificity limits practical applications. To overcome the deficiencies, here we developed Ca 2+ -chelated 2-aminoterephthalic acid on nanosized ceria (Ca-ATPA@CeO 2 ) as an organophosphorus hydrolase mimic. This design integrates stable fluorescence and dual-site catalytic activity to specifically detect methyl-paraoxon (MP) in complex food matrices. The synergy between Ca 2+ (hard Lewis acid) and CeO 2 creates dual active sites to catalyze MP hydrolysis into yellow p -nitrophenol (pNP), and the latter quenches nanozyme fluorescence via inner filter effect. The system enables cross-validated quantification of MP in complex samples, eliminating redox interference through target-specific catalysis. The bimodal “on” colorimetric (pNP color signal) and “off” fluorescence (nanozyme fluorescence intensity) detection achieved linear ranges within 1–200 μM, providing detection limits of 1.43 μM and 0.087 μM, respectively. Our work proposes a reliable strategy for selective MP detection that can avoid redox interference, also providing a simple yet efficient design of high-activity fluorescent hydrolase mimics with broadened applications in food safety analysis and beyond.
研究领域:
食品安全
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Guangxia Yu,Weixiang Wu,Qiang Zhao,Xiaoyun Wei,Qing Lu
This work introduced an efficient immobilization of acetylcholinesterase (AChE) onto amino functionalized carbon nanotubes (CNT–NH 2 ), in order to fabricate high sensitive and practical organophosphorus pesticide (OPs) biosensors. Compared with the pristine, –COOH and –OH decorated CNTs, there were larger amount of enzymes adsorbed on the surface of CNT–NH 2 with a favorable orientation and the best amperometric response was obtained on the AChE/CNT–NH 2 /GC electrode. Furthermore, the biosensor modified with CNT–NH 2 showed a high affinity to acetylthiocholine chloride (ATCh) and could catalyze the hydrolysis of ATCh with an apparent Michaelis–Menten constant ( K m ) value of 67.4 µM. Using paraoxon as a model compound, wide linear ranges from 0.2 nM to 1 nM and 1 nM to 30 nM, and a low detection limit of 0.08 nM were obtained with satisfactory reproducibility and stability. Moreover, the biosensor had also been successfully employed for the determination of low concentrations of pesticides in real vegetable samples. This method could be extended to other functionalized nano–materials for their application in constructing biosensors.
研究领域:
分析化学
·
环境科学
·
生物技术
✓ 已验证
DOI
高影响因子
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
·
2025
·
IF 5.9
Zhenpeng Men,Ragini Singh,Bingyuan Zhang,Santosh Kumar
In this paper, we introduce a seven-core tapered optical fiber biosensor with high sensitivity for label-free detection of paraoxon compounds that utilizes the localized surface plasmon resonance (LSPR) effect. By its unique circle-M-circle (CMC) structure surrounding the fiber surface, it achieves an obvious amplification of evanescent field at the sensor and improves sensitivity greatly. Finally, the optimized tapered shape enhances surface plasmon light interaction and hence increases sensitivity to low concentrations of paraoxon residues. Since gold nanoparticles (AuNPs) are used as an active probe for surface modification, the optical properties of Au make them have a good LSPR effect. We also employ, as auxiliary materials, tungsten disulfide (WS2) thin layers and ZnO nanowires (NWs), of which the biocompatibility advantage can reinforce together with stability for detection probes to the WaveFlex sensor. The results of the experiment show a high sensitivity for detecting paraoxon residues in agricultural products: 33.8 ng/mL and a detection limit of 6.83 μg/mL. The developed biosensor enables portability through miniaturization and also maintains its performance stability in various environmental conditions, which can be considered a powerful tool for field monitoring of paraoxon to enhance both environmental and food safety assessments. The sensor has shown favorable practical applicability due to at least desirable selectivity and repeatability. The CMC-like optical fiber-based WaveFlex biosensor being developed based on LSPR detection represents a great progress of sensor-based technology for environmental monitoring with wide application scenarios, which serves as an efficient and effective surveillance mechanism for point-of-sampling detection to the timely-onset paraoxon analysis.
研究领域:
环境科学
·
生物传感技术
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shaoyang Liu,Mai Luo,Huizhen Liang,Liang Zou,Jiliang Cao,Jinchao Wei,Peng Li
Organophosphorus pesticide residues pose significant risks to human health, but conventional analytical methods are costly and complex. To address this, we developed a new environmentally friendly technique using a simple hydrothermal method to create a ratiometric fluorescent composite (RFC) with two distinct signals: blue and orange. This RFC allowed for rapid detection and quantitative analysis of organophosphorus pesticides. The composite incorporated a cerium-based metal–organic framework (Ce-UiO-66), which could degrade methyl paraoxon (MP) due to the two oxidation states of cerium. The resulting degradation product, p-nitrophenol (p-NP), specifically quenched the blue fluorescence of the RFC via the inner filter effect (IFE), while the orange fluorescence remained unaffected, ensuring consistency under microenvironmental and spectrometer conditions. This innovative approach was sensitive, with a detection limit of 0.55 μM, selective, and repeatable, offering a new strategy for pesticide analysis and safety evaluation of edible and medicinal plant and water samples.
研究领域:
环境科学
·
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）