Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Wenju Liu,Bin Li,Shikun Wu,Shaofeng Wang,Miaomiao He,Hongjuan Bai,Han Wang,Yanmin Shen
Coumaric acid (p-CA), a naturally occurring antimicrobial agent with a broad range of potential applications, is severely limited by its poor water solubility. This study addressed this challenge by encapsulating p-CA within a biocompatible cyclodextrin metal-organic framework (CD-MOF), achieving a maximum drug-loading capacity of 10.217 ± 0.303 %. We performed comprehensive characterization involving X-ray diffraction (XRD), Fourier Transform infrared spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Thermogravimetry Analysis (TGA) and Brunauer Emmett Teller (BET) was undertaken to confirm the successful formation of the inclusion complex. It is noteworthy that the water solubility of p-CA at 25 °C increased 10-fold upon encapsulation, with rapid release achieved within 30 min. Encapsulated p-CA showed enhanced oxidation resistance (increased from 81.527 ± 0.871 % to 85.222 ± 0.871 %), as evidenced by DPPH-scavenging assays, and retained the antimicrobial activity of p-CA (Both p-CA and p-CA/CD-MOF exhibited 100 % antibacterial efficacy against Staphylococcus aureus and approximately 90 % efficacy against Escherichia coli ). Molecular docking and dynamics simulations revealed that p-CA inhibited bacterial glucose transport by occupying the active site of enzyme IIC (EIIC). Furthermore, experiments on the preservation of freshness in strawberries demonstrated the efficacy of p-CA/CD-MOF in maintaining fruit quality over 5 days at 25 °C. These findings emphasize the potential of CD-MOF encapsulation to circumvent the solubility limitations of p-CA, thereby facilitating its expanded use in food preservation and biomedical applications.
研究领域:
食品科学
·
材料科学
·
生物医学工程
·
药物递送
·
抗菌剂研究
✓ 已验证
产品关联
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Xuemei Meng,Tong Zhang,Jun Li,Xiya Cao,Linan Duan,Haitian Fang,Junnan Xu
To overcome the poor solubility and bioavailability of p -coumaric acid ( p -CA), an inclusion complex ( p -CA- β -CD) of p -CA and β -cyclodextrin ( β -CD) was prepared. FT-IR, XRD, DSC, and SEM were performed to characterize the p -CA- β -CD complex. Additionally, the antibacterial activity of p -CA- β -CD against Alicyclobacillus acidoterrestris and the underlying mechanisms were investigated. The results confirmed the successful fabrication of p -CA- β -CD and demonstrated a significant enhancement in p -CA solubility upon complexation with β -CD. p -CA- β -CD effectively inhibited A. acidoterrestris , with an MIC of 1.4 mg/mL, by damaging cell membrane, reducing intracellular pH and ATP, increasing ROS levels, and altering cell morphology. Moreover, 1MIC of p -CA- β -CD caused 2.89 and 2.18 log reductions of A. acidoterrestris during apple juice storage at 4 °C and 25 °C, respectively, without remarkable effect on physicochemical indexes and sensory quality. These findings suggested that p -CA- β -CD could potentially be an emerging and promising natural agent for controlling A. acidoterrestris in juice.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2024
·
IF 3.4
Jiangmei Zheng,Ruobin Sun,Dan Wu,Pengcheng Chen,Pu Zheng
Phenylalanine ammonia-lyase (PAL) plays a pivotal role in the biosynthesis of phenylalanine. PAL from Zea mays (ZmPAL2) exhibits a bi-function of direct deamination of L-phenylalanine (L-Phe) or L-tyrosine(-L-Tyr) to form trans -cinnamic acid or p -coumaric acid. trans -Cinnamic acid and p -coumaric acid are mainly used in flavors and fragrances, food additives, pharmaceutical and other fields. Here, the Activity of ZmPAL2 toward L-Phe or L-Tyr was improved by using semi-rational and rational designs. The catalytic efficiency ( k cat / K m ) of mutant PT10 (V258I/I459V/Q484N) against L-Phe was 30.8 μM −1 s −1 , a 4.5-fold increase compared to the parent, and the catalytic efficiency of mutant PA1 (F135H/I459L) to L-tyrosine exhibited 8.6 μM −1 s −1 , which was 1.6-fold of the parent. The yield of trans -cinnamic acid in PT10 reached 30.75 g/L with a conversion rate of 98%. Meanwhile, PA1 converted L-Tyr to yield 3.12 g/L of p -coumaric acid with a conversion rate of 95%. Suggesting these two engineered ZmPAL2 to be valuable biocatalysts for the synthesis of trans -cinnamic acid and p -coumaric acid. In addition, MD simulations revealed that the underlying mechanisms of the increased catalytic efficiency of both mutant PT10 and PA1 are attributed to the substrate remaining stable within the pocket and closer to the catalytically active site. This also provides a new perspective on engineered PAL.
研究领域:
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Manjia Lu,Shirong Sun,Xiaoya Li,Qiyu Liu,Wenli Zhang,Xueqing Qiu
Two distinctive aromatic units, p − coumarate and ferulate, exist in corncob lignin, which have the potential to yield p − coumaric acid ( p CA) and ferulic acid (FA). Although p CA and FA are primarily extracted from corncob lignin utilizing strong acids and bases, extremely acidic or alkaline conditions result in the disruption of the aromatic unit structure of the residual lignin. Herein, lactic acid coupled with choline chloride was utilized as acidic deep eutectic solvent (DES), while K 2 CO 3 with glycerin was used as alkaline DES, thereby facilitating the extraction of p CA, FA and lignin from corncob in a mild environment. Furthermore, the differences in monophenol yields from pyrolysis of lignin were investigated. The alkaline DES exhibited a stronger extraction capacity for these acids. The yields of p CA and FA were 15.47 mg/g and 7.44 mg/g (based on the weight of corncob). Contrastively, the subsequent pyrolysis process yielded a higher amount of monophenol from the lignin extracted using acidic DES, with notably greater quantities of low methoxy phenolic monomers. This renders it a preferred option for subsequent processing into high−calorific biofuels. This work presents a straightforward and efficient strategy for the deconstruction of the lignin from corncob to enhance the utilization value of agricultural wastes.
研究领域:
生物质化学
·
能源科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2022
·Vol. 10
·期 11
·2269
·
IF 4.926
Yan-Lu Wang,Wei-Kang Wang,Qi-Chao Wu,Fan Zhang,Wen-Juan Li,Sheng-Li Li,Wei Wang,Zhi-Jun Cao,Hong-Jian Yang
In plant cell wall, ferulic acid (FA) andp-coumaric acid (pCA) are commonly linked with arabinoxylans and lignin through ester and ether bonds. These linkages were deemed to hinder the access of rumen microbes to cell wall polysaccharides. The attachment of rumen microbes to plant cell wall was believed to have profound effects on the rate and the extent of forage digestion in rumen. The objective of this study was to evaluate the effect of bound phenolic acid content and their composition in corn silages on the nutrient degradability, and the composition of the attached bacteria. Following an in situ rumen degradation method, eight representative corn silages with different FA andpCA contents were placed into nylon bags and incubated in the rumens of three matured lactating Holstein cows for 0, 6, 12, 24, 36, 48, and 72 h, respectively. Corn silage digestibility was assessed by in situ degradation methods. As a result, the effective degradability of dry matter, neutral detergent fibre, and acid detergent fibre were negatively related to the ether-linked FA andpCA, and their ratio in corn silages, suggesting that not only the content and but also the composition of phenolic acids significantly affected the degradation characteristics of corn silages. After 24 h rumen fermentation, Firmicutes, Actinobacteria, and Bacteroidota were observed as the dominant phyla in the bacterial communities attached to the corn silages. After 72 h rumen fermentation, the rumen degradation of ester-linked FA was much greater than that of ester-linkedpCA. The correlation analysis noted thatErysipelotrichaceae_UCG-002,Olsenella,Ruminococcus_gauvreauii_group,Acetitomaculum, andBifidobacteriumwere negatively related to the initial ether-linked FA content whilePrevotellawas positively related to the ether-linked FA content and the ratio ofpCA to FA. In summary, the present results suggested that the content of ether-linked phenolic acids in plant cell walls exhibited a more profound effect on the pattern of microbial colonization than the fibre content.Keywords:corn silage;rumen-attached bacteria;phenolic acids;in situ degradation
研究领域:
动物营养学
·
微生物学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
Cell Host & Microbe
·
2024
·
IF 20.6
Dongping Li,Meijuan Wan,Lanfeng Xue,Zhelin Zhang,Yifeng Qiu,Fengyi Mei,Niexing Tang,Chunxiao Yu,Yao Yu,Tianqi Chen,Xing Ding,Qin Yang,Qiuyan Liu,Peng Gu,Wei Jia,Yu Chen,Peng Chen
Cholestatic liver disease (CLD) is a common liver disorder with limited treatment options. Here, we demonstrate that zinc (Zn) supplementation can alter the gut microbiome to mitigate cholestatic liver injury. Oral Zn altered the microbiota of mice and humans (this study was registered at clinicaltrials.gov [NCT05597137]), increasing the abundance of Blautia producta ( B. producta ) and promoting the generation of p-coumaric acid. Additionally, p-coumaric acid concentrations were negatively correlated with liver injury parameters in CLD patients. In mice, the protective effects of Zn were partially mediated by p-coumaric acid, which directly bound to nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2) and suppressed the production of reactive oxygen species (ROS) in hepatocytes, thus preventing hepatocyte cell death and liver damage. Additionally, knocking out the histidine ammonia-lyase, which catalyzes the conversion of tyrosine to p-coumaric acid in B. producta , blunted the protective effects of Zn. These findings highlight a host-microbiota interaction that is stimulated by Zn supplementation, providing potential benefits for CLD.
研究领域:
胃肠病学
·
微生物学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Süreyya Oğuz Tümay,Vildan Sanko,Ahmet Şenocak,Yasin Orooji,Erhan Demirbas,Yeojoon Yoon,Alireza Khataee
Phenolic compounds that are naturally found in food samples are not only an important part of the human diet but also useful bioactive substances for health. Among these, para- coumaric acid ( p- CA) has antibacterial and antioxidant properties and is used in many industrial processes. In this study, the novel MAX-phase material, Nb 4 AlC 3 , was successfully prepared and characterized in detail with various spectroscopic, microscopic and thermal techniques. The sensor performance of Nb 4 AlC 3 modified glassy carbon electrode (Nb 4 AlC 3 @GCE) was evaluated and analytical parameters were calculated. Experimental conditions such as pH and amount of modifier were optimized with differential pulse voltammetry (DPV) measurements. The real samples analyses of lemon, apple and pomegranate were applied for determination of p- CA with Nb 4 AlC 3 @GCE sensing system under the optimized conditions. The accuracy was evaluated by spike/recovery and high-performance liquid chromatography analysis, which accounted for high accuracy of the Nb 4 AlC 3 @GCE sensing system. The limit of detection, limit of quantification, linear working range and relative standard deviation (%) of the Nb 4 AlC 3 @GCE sensing system were determined as 0.28 and 0.85 µmol/L, 0.8–80.0 µmol/L, 3.17 %, respectively. The results showed that the proposed sensing system has the high precision at lower concentration of p- CA.
研究领域:
分析化学
·
食品科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Zhu Xuanquan,Jia Meng,Zi Dingchun,Zhou Peng,Du Yu,Wang Na,Dai Huijuan,Wang Ge,Bai Yuxiang
Introduction: Applying biochar (BC) to reduce toxic substance accumulation in soil, either through direct adsorption or modulation of the microbial community, has received considerable attention. However, a knowledge gap exists regarding how BC regulates microbial community structure and functions to mitigate toxic substance accumulation.Methods: We previously identified p-coumaric acid (p-CA) as a representative autotoxin in tobacco rhizosphere soil. On this basis, this study simulated a soil environment with p-CA accumulation to investigate the impacts of BC on p-CA, soil physicochemical properties, and microbial community structure and function.Results: The results showed that p-CA could be directly adsorbed onto BC, which followed the pseudo-second-order kinetic model (R2 = 0.996). A pot experiment revealed that BC significantly reduced soil p-CA, altered soil microbial composition, and enhanced bacterial community diversity. A weighted correlation network analysis showed a close association between taxon 1 in the microbial network and p-CA, suggesting a pivotal role for this taxon in reducing p-CA, with Devosia and Nocardioides identified as potential key contributors to this process. The prediction of possible keystone taxa functions showed that BC increased the relative abundances of aromatic compound degraders. Mantel tests indicated that soil organic matter exerted the greatest influence on keystone taxa functions and hub genera.Discussion: These findings suggest that BC may either directly chemisorb p-CA or indirectly facilitate p-CA degradation by regulating the functioning of keystone taxa. The results of this study provide a novel perspective for further investigation of the mechanisms through which BC reduces the accumulation of toxic substances in soil.
研究领域:
环境科学
·
土壤学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Tongjun Liu,Li Yang,Bin Liu,Liping Tan
Hydroxycinnamic acids released during alkaline pretreatment of lignocellulose, especially p- coumaric acid ( p -CA) and ferulic acid (FA), negatively affect the enzymatic hydrolysis and fermentation. However, the mechanism of this effect is not well understood. In this study, we investigated the releasing behavior and negative effect and examined the underlying mechanisms. The results demonstrated that the hydrogen peroxide concentration and biomass loading affected the release of p -CA and FA significantly during alkaline hydrogen peroxide (AHP) pretreatment of corn stover. p -CA and FA mainly inhibited the activity of endoglucanase, xylanase, and filter paper activity (FPA), while the amount of lignocellulolytic enzyme protein was not affected. Thus, they acted as inhibitors of enzyme activities. Molecular docking study indicated that p -CA and FA inhibited the lignocellulolytic enzyme activity and enzymatic hydrolysis efficiency mainly by forming non-covalent bonds to specific amino acids in the active sites of the lignocellulolytic enzymes.
研究领域:
生物化学
·
生物技术
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Jingna Liu,Yuanhong Zhuang,Yonghua Hu,Shan Xue,Hui Li,Lian Chen,Peng Fei
In this work, p- coumaric acid and caffeic acid were grafted on blueberry anthocyanins through enzyme catalysis method to improve their colour stability and antioxidant activity . The acylation degrees of p- coumaric acylated anthocyanins (Co-An) and caffeic acid anthocyanins (Ca-An) reached 5.38% and 5.68%, respectively. The results from Fourier transform infrared spectroscopy , X-ray photoelectron spectroscopy, ultraviolet visible spectroscopy and hydrogen nuclear magnetic resonance implied that p- coumaric acid and caffeic acid grafted on the 6-OH of glucoside and galactoside and the 5-OH of arabinose through esterification reaction. As revealed in the in vitro experiment on antioxidation activity and the experiment on colour stability, Co-An and Ca-An showed stronger antioxidant activity in DPPH assay and β-carotene bleaching assay and higher colour stability during storage at 25 °C, 40 °C and 60 °C than native blueberry anthocyanins.
研究领域:
食品科学
·
化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2020
·Vol. 23
·期 2
·838-847
·
IF 9.48
Mingtao Zhao,Xulin Hong,Abdullah,Ruilian Yao,Yi Xiao
Biomass-derived hydroxycinnamates (mainly including p-coumaric acid and ferulic acid), which are natural sources of aromatic compounds, are highly underutilized resources. There is a need to upgrade them to make them economically feasible. Value-added phenolic glycosides and their derivatives, both belonging to a class of plant aromatic natural products, are widely used in the nutraceutical, pharmaceutical, and cosmetic industries. However, their complex aromatic structures make their efficient biosynthesis a challenging process. To overcome this issue, we created three novel synthetic cascades for the biosynthesis of phenolic glycosides (gastrodin, arbutin, and salidroside) and their derivatives (hydroquinone, tyrosol, hydroxytyrosol, and homovanillyl alcohol) from p-coumaric acid and ferulic acid. Moreover, because the biomass-derived hydroxycinnamates directly provided aromatic units, the cascades enabled efficient biosynthesis. We achieved substantially high production rates (up to or above 100-fold enhancement) relative to the glucose-based biosynthesis. Given the ubiquity of the aromatic structure in natural products, the use of biomass-derived aromatics should facilitate the rapid biosynthesis of numerous aromatic natural products.
研究领域:
生物化学
·
生物技术
·
药学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiang-Rong Cheng,Jia-Hui Ma,Issoufou Amadou,Wei Zhao,Yu-Yao Chen,Chen-Xi Zhang,Bin Guan
Xiaoheiyao is the rhizome of Inula nervosa Wall., a traditional spice and medicinal herb in China. In this study, the creatinine inhibitor from Xiaoheiyao extract and also the effects and mechanism on the production of heterocyclic aromatic amines (HAAs) were investigated. Xiaoheiyao extract inhibited the total contents of seven detected HAAs in grilled beef patties, particularly aminoimidazole-azaarenes (AIAs) in a dose-dependent manner, reaching a maximum inhibition rate of 62% for total HAAs and 73% for AIAs. The most effective subfraction of Xiaoheiyao extract (IER80) contained abundant potential creatinine inhibitors, as revealed by immobilized creatinine probe, HPLC and UPLC-MS/MS analyses. Moreover, electrophilic p -coumaric acid derivatives were discovered from IER80 by feature based molecular networking. p -Coumaric acid was demonstrated to inhibit the contents of total HAAs and AIAs in grilled beef patties and model system. Quantitative analyses of the precursor and intermediates of AIAs in model system revealed that p -coumaric acid mainly affected the generation of AIAs by inhibiting creatinine.
研究领域:
食品科学
·
药理学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）