RSC Advances
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2015
·Vol. 5
·期 82
·67093-67101
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IF 3.84
Yuping Wei,Jiandong Xu,Liang Zhang,Yankai Fu,Xia Xu
The huge demand for the most promising biopharmaceuticals, monoclonal antibodies (mAb), has resulted in a need for more efficient and less costly downstream purification processes for mAb. The currently used Protein A Agarose is unattractive due to toxic ligand leakage and high cost. In this study, three novel small peptide ligands, DWHW, CEWW and HEYW, were designed based on the interactions with human immunoglobulin G (IgG) using molecular simulations. The effects of pH, ionic strength and flow rate on the binding capacities to IgG were investigated. The static and dynamic binding capacities were determined. The dissociation constant (Kd) of the DWHW resin was 1.1 × 10−5 M. The binding capacity of the DWHW resin was 24.5 mg ml−1, which is comparable to Protein A Agarose. The DWHW resin was able to purify IgG from cMEM and CHO cell culture supernatants with a purity of more than 95% under elution conditions of pH 9 (50 mM sodium borate buffer) and pH 3 (50 mM glycine–HCl buffer). The results indicate that the small peptide ligands, especially DWHW, can offer a potential alternative for mAb purification.
研究领域:
生物化学
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生物工程
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制药工程
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产品关联
DOI
高影响因子
Food Production Processing and Nutrition
·
2025
·Vol. 7
·期 1
·1-14
·
IF 5.7
Sun Teng,Wang Zhaojun,Dai Yiqiang,Zhai Kang,Fang Xiangna,Chen Yitian,Zhao Baomin,Dong Mingsheng
In this study, Rhizopus oligosporus RT-3 was utilized to ferment soybean meal to obtain a tempe-like food with low fat and high bioactivity. To investigate the impact of fermentation on soybean meal’s nutritional value, we detected the protein-related indices and conducted a non-targeted metabonomic analysis on both fermented soybean meal (FSM) and soybean meal (SM) in order to explore changes in a certain type of metabolite and its corresponding metabolic pathways. The results showed a significant increase in crude protein, peptides and amino acids while getting porous after fermentation. A reduction of raffinose and stachyose to 18.1% and 8.5%, respectively, due to the action of α-galactosidase and β-furanofructosidase occurred. As to amino acid metabolites, essential amino acids for human bodily functions such as lysine, histidine, and threonine were significantly upregulated. Furthermore, nine peptide metabolites were identified as angiotensin-converting enzyme (ACE) inhibitors and dipeptidyl peptidase-4 inhibitors (DPP-IV). After feeding with different doses of FSM, levels of insulin-like growth factor-1 (IGF-1), hemoglobin (HB), albumin (ALB), and total protein (TP) of malnourished mice all returned to normal. In conclusion, fermentation with Rhizopus oligosporus RT-3 can improve the quality of SM by reducing the level of oligosaccharide anti-nutrition factors and increasing the contents of free amino acid and small bioactive peptides, which provide a theoretical basis and new ideas for the application of FSM. Graphical
研究领域:
食品科学
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代谢组学
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营养学
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DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Ruixia Chen,Xiao-Chen Liu,Xianqi Yao,Wei Wang,Junyi Xiang,Igor Tomasevic,Weizheng Sun
BACKGROUND High-sodium intake has been proven to bring serious risks to public health. A potential sodium substitute of salt taste-enhancing hydrolysate (STEH) of protein has been focused on recently. The salt taste-enhancing activity (STEA) of STEH still needs to be improved. High-pressure and calcium chloride (CaCl 2 ) pretreatments were reported to affect proteolysis and promote the release of bioactive peptides. Hence, we investigated effects of high-pressure and CaCl 2 pretreatments on hydrolysis and STEA of STEH derived from spent hen. RESULTS The pretreatments significantly influenced STEA of spent hen meat hydrolysate (SHH), especially 200 MPa pressure and 80 mmol L −1 CaCl 2 pretreatments increased 27.1% salt taste intensity of SHH compared to that of blank (without pretreatments) according to sensory evaluation, the SHH umami also increased after pretreatments. In SHH, the proportion of peptides < 1000 Da increased up to 79.37% after the pretreatments compared to 73.68% of the blank. The degree of hydrolysis (DH) increased to 19.45% for moderate high-pressure (200 MPa) from 18.02% for blank, and the DH decreased after higher high-pressure and CaCl 2 pretreatments, especially for CaCl 2 in 80 mmol L −1 . The change in particle size distribution of SHH has similar trends to DH. CONCLUSION High-pressure and CaCl 2 pretreatments increased STEA of SHH by affecting hydrolysis process. The STEA increase may be related to increased small-peptide proportion in SHH. Meanwhile, moderate high-pressure may promote protein unfolding and further increase DH according to particle size distribution of SHH. The combination of proteolysis and pretreatments of high-pressure and CaCl 2 is a promising method to produce STEH. © 2024 Society of Chemical Industry.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Meng Xu,Zhenwei Wu
In this study, three small peptoids with different structures, named Sil-peptoids, were developed to improve the separation selectivity of zwitterion-exchange/reversed-phase mixed-mode chromatography stationary phases for multi-component complex drugs. Nonpolar, amphoteric, and alkaline drugs were used as test samples to demonstrate their retention behaviors in reversed-phase, ionic, and mixed-mode interactions. It was observed that different carboxyl anions in the small peptoids of the Sil-peptoids had vast differences in their stereo-selectivity. The stereo-selectivity and the influence of Sil-peptoids on the retention behavior of complex drugs and their interaction mechanism for the drug molecules were effectively evaluated through the combination of chromatographic analysis and molecular modeling. Finally, a mixture of drugs consisting of two polar and six non-polar drugs was used to obtain a separation effect with a resolution >1.5. Two other groups of polar antibiotics were used to verify the separation ability of the Sil-peptoids. The results indicated that the Sil-peptoids could separate multiple substances simultaneously. These novel stationary phases can be applied to the analysis of complex multi-component drugs.
研究领域:
分析化学
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药物化学
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生物医学工程
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Xiangyu Wang,Donghai Xia,Hai Han,Kun Peng,Peijie Zhu,Jacques Crommen,Qiqin Wang,Zhengjin Jiang
The rapid development of monoclonal antibodies (mAbs) in therapeutic and diagnostic applications has necessitated the advancement of mAbs purification technologies. In this study, a biomimetic small peptide ligand 3,5-di - tert-butyl-4-hydroxybenzoic acid-Arg-Arg-Gly (DAAG) functionalized monolith was fabricated through a metal ion chelation-based multi-step approach. The resulting monolith showed good chromatographic performance. Compared with the Ni 2+ based IMAC monolith, the DAAG functionalized monolith exhibited not only excellent specificity but also higher dynamic binding capacity (DBC). The 10% DBC and 50% DBC for hIgG reached as high values as 26.0 and 34.6 mg/mL, respectively, at a ligand density of 8.8 μmol/mL, due to the high porosity and accessibility of the monolithic matrix. Moreover, the stability of the DAAG functionalized monolith in successive breakthrough experiments indicates that it has a promising potential for long-term use in mAbs purification. Finally, the DAAG functionalized monolith was successfully applied to the purification of trastuzumab or human immunoglobulin G (hIgG) from biological samples.
研究领域:
生物技术
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药学
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化学工程
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分析化学
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DOI
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Wenjing Su,Wenhao Xu,Nikolay E. Polyakov,Alexandr V. Dushkin,Pei Qiao,Weike Su
In this study, a moderate method for efficient zero-waste utilization and reforming from shrimp shell waste was developed by means of mechanochemistry. The method enables the conversion of shrimp shell waste into astaxanthin , chitin , small peptides, amino acids, and lipids for zero waste; the method uses green mechano-enzymatic hydrolysis and mechanical solid organic acid treatment of shrimp shell waste to achieve zero consumption of strong acids alkali, and organic solvent. The underlying mechanism was revealed by thermodynamic determination, spectroscopic analysis , and electron microscopy observation of the extraction process. In the research, a green economic reuse approach for crustacean waste is proposed to achieve United Nations Sustainable Development Goals 3, 6, 12 and 14 with the concept of circular economy(Sutherland et al., 2021), and this study proposes a green economic reuse method of crustacean waste.
研究领域:
环境科学
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化学
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材料科学
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可持续性
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）