SCIENCE OF THE TOTAL ENVIRONMENT
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2020
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IF 6.551
Aiyun Guo,Chengrong Pan,Jinyu Ma,Yanyu Bao
Rhizosphere is a crucial site for the proliferation of antibiotic resistance genes (ARGs) in agricultural soil. Pesticide contamination is ubiquitous in soil, such as chlorpyrifos as one of the most commonly used pesticides. However, limited knowledge is reported about ARGs profiles changes and the driving mechanism of ARGs prevalence in rhizosphere soil after adding pesticide. In this study, irrespective of chlorpyrifos presence, the abundances of ARGs ( tetM , tetO , tetQ , tetW , tetX , sul1 and sul2 ) and intI1 in rhizosphere soil of wheat were obviously higher than those in bulk soil. 20.0 mg·kg −1 chlorpyrifos significantly increased the abundance of total ARGs and intI1 in bulk soil, respectively, at day 50 and 100, but not in rhizosphere soil. Rhizosphere influence on ARGs was far greater than chlorpyrifos. ARGs and intI1 abundances were higher at day 50 than ones at day 100. C/N ratio and NO 3 − -N content, which were affected by rhizosphere and cultivation time, significantly explained the increased ARGs. Compared to bulk soil, rhizosphere shifted host bacteria of tetracycline resistance genes (TRGs), intI1 at genus level, and host bacteria of sul1 , sul2 at phylum level. Rhizosphere simplified the linkage of ARGs, host bacteria and metabolites. Bacterial communities played important roles in the variation of ARGs and intI1 , and the difference in the distribution of potential hosts between bulk and rhizosphere soil was related to metabolites abundance and composition. These results provide valuable information for understanding the linkage of ARGs, associated bacteria communities and metabolites in the wheat rhizosphere soil.
研究领域:
环境微生物学
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土壤科学
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生态毒理学
✓ 已验证
DOI
PubMed
高影响因子
Rhizosphere
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2024
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IF 3.4
Tengteng Shao,Huaqing Liu,Aiyun Guo,Dongle Cheng,Xinhan Chen,Shugang Hu,Jian Zhang
Rhizosphere microorganisms are crucial for the growth and health of their host plants. Dicamba is one of the representative herbicides used for the control of wheat weeds. In recent years, the usage of dicamba has escalated globally, particularly with the advent of dicamba-tolerant transgenic crops. However, the impacts of this herbicide on non-target organisms, particularly the rhizosphere microorganisms of wheat, is not yet clear. Furthermore, rhizosphere microorganisms are important for nitrogen cycling in soil-crop systems, the influence of dicamba on rhizosphere nitrogen cycling functionality needs further investigation. This study aims to elucidate the effects of dicamba on the microbial community, metabolite profiles, and nitrogen cycling-related genes in wheat rhizosphere. Our findings indicated that dicamba led to significant alterations in the diversity and co-occurrence patterns of the rhizosphere microbial community, notably reducing the abundances of phyla Verrucomicrobia, Planctomycetes, and Cyanobacteria. Additionally, dicamba markedly disturbed the rhizosphere metabolite profiles, particularly those associated with carbohydrate and amino acid metabolism. Notably, dicamba treatment resulted in an up-regulation of enzymatic genes catalyzing processes of assimilatory nitrate reduction to ammonium (ANRA)/dissimilatory nitrate reduction to ammonium (DNRA) (EC: 1.7.5.1, EC: 1.9.6.1, and EC: 1.7.1.15) and denitrification (EC: 1.7.2.1 and EC: 1.7.2.5). The observed increases in the abundances of the genera Cellulosimicrobium and Pseudomethylobacillus were critical for facilitating ANRA and DNRA. The application of dicamba altered soil organic matter content, which in turn influenced the abundances of microorganisms involved in ANRA/DNRA and denitrification, thereby affecting the nitrogen cycling functionality in the rhizosphere. This study offers valuable theoretical support for assessing the rhizosphere ecological effects of agrochemical application.
研究领域:
环境微生物学
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土壤学
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农学
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生态学
✓ 已验证
产品关联
DOI
高影响因子
Biomed Research International
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2019
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IF 2.197
Xiaohui Wang,Qian Li,Junkang Sui,Jiamiao Zhang,Zhaoyang Liu,Jianfeng Du,Ruiping Xu,Yanyan Zhou,Xunli Liu
Soilborne pathogens affect plant growth and food production worldwide. The application of chemical fertilizers and pesticides to control plant diseases has harmful effects; fortunately, plant growth-promoting rhizobacteria can be used as a potential alternative strategy. Here, Paenibacillus jamilae HS-26 was selected for its highly antagonistic activity against several soilborne pathogens. The bacterium synthesized hydrolytic enzymes and released extracellular antifungal metabolites and volatile organic compounds—primarily, N, N-diethyl-1, 4-phenylenediamine, which was detected by gas chromatography-mass spectrometry and shown to inhibit fungal mycelial growth. Furthermore, HS-26 was useful for nitrogen fixation, phosphate and potassium solubilization, and siderophore and indoleacetic acid production. In vitro tests and pot experiments revealed that HS-26 considerably increased plant biometric parameters. Illumina MiSeq sequencing data showed a significant reduction in soilborne pathogens and increase in beneficial bacteria in the wheat rhizosphere after treatment with strain HS-26.
研究领域:
植物病理学
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微生物学
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农业科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）