CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2013
·
IF 0.769
Jian-Ming JI,Xiu-Xia HE,Qian DUAN,Zhen-Xin WANG
Uniformly porous silicon substrate with large surface area (12 mm × 58 mm) was prepared by double-cell electrochemical etching method. After functionalized with epoxide groups, the porous silicon substrate can be employed to fabricate protein microarray. In proof of principle experiment, well-known biomolecule recognition system (the interaction of mouse IgG with rabbit anti-mouse IgG antibody) was chosen to establish the new microarray format. It was found that the detection sensitivity of the mouse IgG microarray was dependent on preparing conditions of porous silicon substrate including etching current density, etching time, and concentration of HF. Under the optimal conditions (500 mA cm −2 etching current density, 450 s etching time and 25% HF), specific binding could be detected using rabbit anti-mouse IgG antibody at concentrations as low as 10 ng mL −1 with a dynamic range from 316 ng mL −1 to 10 μg mL −1 . The results demonstrate that the as-prepared porous silicon substrate could be used to fabricate the protein microarray with high reaction capability.
研究领域:
生物技术
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Lizhong Zhang,Qiang Huang,Xiaonan Zhang,Zhen Zeng,Hailong Zhang,Tian Guan,Yang Xu,Chongqi Zhou,Lingqin Meng,Gengyu Liang,Zhangyan Li,Bei Wang,Le Liu,Cuixia Guo,Yonghong He
In this study, an interfacial biosensing scheme with ultra-precision is proposed. The scheme uses weak measurement techniques to ensure ultra-high sensitivity of the sensing system while improving the stability of the system through self-referencing and pixel point averaging, thus achieving ultra-high detection accuracy of biological samples. In specific experiments, we have used the biosensor in this study to perform specific binding reaction experiments for protein A and Mouse IgG with a detection line of 2.71 ng/mL for IgG. In addition, the sensor is non-coated, simple in structure, easy to operate, and low in cost of use.
研究领域:
生物传感器
·
生物技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 20
·6861-6868
·
IF 7.059
Peili Zhao,Yaofei Chen,Yu Chen,Shiqi Hu,Hui Chen,Wei Xiao,Guishi Liu,Yong Tang,Jifu Shi,Zhendan He,Yunhan Luo,Zhe Chen
Biosensors based on surface plasmon resonance (SPR) have shown significant applications in drug screening, environmental monitoring, immunoassay, etc.; however, improving the sensitivity of these biosensors is one of the prime tasks at present. In this study, taking advantage of the special optical and morphological characteristics of MoS2 nanoflowers, we synthesized a sensitivity-improved SPR biosensor by successively depositing MoS2 nanoflowers and gold nanoparticles (AuNPs) on a traditional SPR sensor and then applied this biosensor to the immunoassay of mouse IgG. It was found that the deposition of the MoS2 nanoflowers could improve the bulk refractive index sensitivity from 2212.2 to 2857.2 nm per RIU (RIU: refractive index unit); however, the further addition of AuNPs resulted in a decrease in the bulk refractive index sensitivity to 2149.7 nm per RIU. Moreover, when the proposed biosensor was applied to the IgG immunoassay, a 0.047 nm (μg mL−1)−1 sensitivity of the MoS2–AuNP-modified sensor was achieved, which was ∼3 times that of the unmodified sensor (0.016 nm (μg mL−1)−1). This improvement in the sensitivity of the biosensor towards IgG can be attributed to the flower-like morphology of MoS2 that provides more sites for the loading of AuNPs, thus facilitating the immobilization of a larger amount of IgG antibody on the surface of the sensor. Because of its simple fabrication process and compatibility with the standard bio-activation method of gold surface, the proposed biosensor provides an easily-accessible platform for biological analysis and detection with improved sensitivity.
研究领域:
生物技术
·
纳米技术
·
分析化学
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
POLYMER BULLETIN
·
2021
·Vol. 79
·期 8
·6409-6429
·
IF 2.87
Ding Guanjun,Wang Ao,Shi Xinming,Li Jumei,You Lijun,Wang Shaoyun
Polyphosphazene microspheres (PZS) multispectral encoded by fluorescence and surface enhanced Raman spectra (SERS) were prepared and used as solid phase carrier of capture antibody for antibody detection. A variety of fluorescent encoded polyphosphazene microspheres (FPZS) incorporating single and multiple fluorescent molecules were prepared. Multiple SERS encoded polyphosphazene microspheres (SPZS) were obtained by modification of different Raman reporters on the surface of PZS@Ag, which were fabricated with rough morphology through in situ deposition of Ag nanoparticles. Fluorescence and SERS spectral combined encoded polyphosphazene microspheres (FSPZS) were prepared by encoding of fluorescent molecules and SERS reporters. The optical signals in the two independent detection channels were separable and ensured the accuracy for the encoding method. Immunoassays of goat IgG, rabbit IgG and mouse IgG were performed by the as-prepared FPZS, SPZS and FSPZS as solid phase carrier of capture antibody and fluorescent labeled second antibody, using sandwich immunoassay method. The immunoassay results showed that the multiple-spectra encoded microspheres have stable and distinguishable encoding signals, large encoding volume and multiplexed analytical capability in sandwich immunoassays.
研究领域:
材料科学
·
分析化学
·
生物传感器
·
免疫学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
He Zhou,Huan Yue,Yali Zhou,Lin Wang,Zhifeng Fu
Novel amorphous carbon nanoparticles (ACNPs) with high electrochemiluminescence (ECL) quenching efficiency were prepared from candle soot with a quite simple approach. The prepared ACNPs were employed as novel energy quenchers for an ECL immunosensor on a disposable screen-printed carbon electrode array (SPCEA) substrate. Gold nanoparticles-doped chitosan composite film was utilized to immobilize Ru(bpy) 3 2+ -labeled antibody on the SPCEA, and ACNPs were linked with antigens to obtain ACNP-antigen conjugate. The immunoreaction between the Ru(bpy) 3 2+ -labeled antibody and the ACNP-antigen conjugate bridged the Ru(bpy) 3 2+ donor and the ACNPs acceptor, which led to quenching of ECL emission from Ru(bpy) 3 2+ via energy transfer. In the presence of the target antigen, this antigen competed with the ACNP-antigen for the immobilized Ru(bpy) 3 2+ -labeled antibody, thus led to decreased occurrence of the ECL quenching. This ACNPs-based ECL quenching system was successfully applied to detect mouse IgG, which was used as a model analyte. The method showed a linear range of 0.50–400 ng mL −1 and a detection limit of 0.35 ng mL −1 . The results for real sample assay and recovery test demonstrated the reliability of this ECL quenching-based immunosensor. It could be further extended to multiplexed immunoassay of biomarker proteins for clinical diagnosis.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
临床诊断
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Hui Ouyang,Mengyao Wang,Wenwen Wang,Zhifeng Fu
Luminol-reduced gold nanoparticles (LuReGNPs) were prepared and utilized as dual-response signal probes for the development of a colorimetric/chemiluminescent immunochromatographic test strip (ITS). LuReGNPs-labeled antibody was adopted as the signal tracer to conduct immunoassay of mouse IgG using the developed ITS device. After 6-min competitive immunoreaction, the tracer antibody was captured on the test zone, which led to accumulation of LuReGNPs. The appearance of red color resulting from LuReGNPs accumulation on the test zone was observed by naked eyes as a qualitative and semi-quantitative signal. Moreover, luminophore in the signal probes enabled sensitive quantitation after initiating the luminol-H 2 O 2 chemiluminescent reaction. Under the optimal conditions, mouse IgG was detected within a linear range of 0.10–100 ng/mL with a detection limit of 0.033 ng/mL (S/N = 3). The results for real biological samples detection and recovery tests demonstrated the acceptable reliability of the dual-response ITS. It exhibited great potential in both rapid screening and sensitive quantitation. Due to the merits of facile manipulation, short detection time and ideal portability, the platform could be further extended to multianalyte immunoassay of biomarker proteins for point-of-care test.
研究领域:
分析化学
·
纳米技术
·
生物医学工程
·
即时检测
✓ 已验证
产品关联
DOI
高影响因子
Journal of Immunotoxicology
·
2022
·
IF 3.439
Wenhua Zhong,Penghuan Chang,Lianfang Gan,Lifan Zhong,Zhaoxin Yang
Most current methods to assess T-cell-dependent antibody responses (TDAR) are semi-quantitative and based on measures of antibody titer generated against a standard antigen like keyhole limpet hemocyanin (KLH). The precision, sensitivity, and convenience of TDAR assays might be improved by applying rapid, sensitive, specific cytometric bead assays (CBA). In the study here, KLH antigen was covalently coupled onto the surface of cytometric beads using immune microsphere technology, and IgM antibody capture spheres were prepared for use in pretreatment processing of samples. The working parameters associated with this novel TDAR-CBA system were optimized in orthogonal experiments. The optimal concentration of the KLH coating solution in this system was 160 μg/ml, that of the anti-KLH IgG capture spheres 6.0 × 105/ml, and the optimal dilution of fluorescein isothiocyanate (FITC)-conjugated Affini-Pure Goat Anti-Mouse IgG (H + L) was 60 μg/ml. Repeated tests indicated that this approach yielded good linearity (r2 = 0.9937) method, with a within-run precision of 3.1–4.9%, and a between-run precision of 4.4–4.9%. This new approach had a limit of detection of 113.43 ng/ml (linear range = 390.63–50 000), and an interference rate of just 0.04–3.51%. Based on these findings, it seems that a new mouse TDAR assay based on CBA can be developed that would appear to be more sensitive, accurate, and precise than the current TDAR assay approaches based on traditional ELISA.
研究领域:
免疫学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2018
·
IF 4.019
Yu Guanliu,Yu Xianglong,Yang Guoping,Tang Yi,Diao Youxiang
Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus that has resulted in large economic losses to the duck-rearing industry in China since 2010. Therefore, an effective diagnostic approach to monitor the spread of DTMUV is necessary. Here, a novel diagnostic immunochromatographic strip (ICS) assay was developed to detect DTMUV. The assay was carried out using colloidal gold coated with purified monoclonal antibody A12D3 against envelope E protein. Purified polyclonal C12D1 antibodies from BALB/c mice against the envelope E protein were used as the capture antibody. Goat anti-mouse IgG was used to detect DTMUV, which was also assembled on the ICS. Results showed that the ICS could specifically detect DTMUV within 10 min. It also could be stored 25 and 4°C for 4 and 6 months, respectively. The sensitivity of the ICS indicated that the dilution multiples of positive allantoic fluid of DTMUV (LD50: 104.33/0.2 ml) was up to 200. Its specificity and sensibility showed no significant change under the above storage situations. Fifty clinical samples were simultaneously detected by ICS and reverse-transcription polymerase chain reaction with a 93.9% coincidence rate between them. It proved that the ICS in the present study was highly specific, sensitive, repeatable, and more convenient to rapidly detect DTMUV in clinical samples.
研究领域:
兽医学
·
病毒学
·
诊断技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 9
·2624-2631
·
IF 3.969
Yuanyuan Chen,Hanwen Cheng,Kha Tram,Shengfeng Zhang,Yanhua Zhao,Liyang Han,Zengping Chen,Shuangyan Huan
In this study, a novel paper-based SERRS immunoassay based on magnetic separation and alkaline phosphatase (ALP) enzyme catalyzed hydrolysis reaction was developed. By using modified antibodies conjugated to magnetic beads, capture of mouse IgG followed by addition of ALP-labeled antibodies would form a sandwich-like immunoconjugate. After magnetic separation, 5-bromo-4-chloro-3-indolyl phosphate (BCIP), a low SERRS active compound, was added as the substrate for ALP to generate a high SERRS response. Detection was conducted on a silver colloid/PVP/filter paper SERS substrate by spotting a pre-aggregated silver colloid sol onto polyvinyl pyrrolidone (PVP) modified filter paper using a semi-automatic TLC sample applicator. The optimization of the highly SERS active paper-based substrate, dynamic hydrolysis process of BCIP, quantitative detection of IgG, and selectivity of the assay was studied in detail. By taking advantage of magnetic separation in order to decrease the background interference, the selective enzyme reaction involved in producing a highly SERRS active product could detect mouse IgG from 1 to 500 ng mL−1 with a LOD of 0.33 ng mL−1.
研究领域:
分析化学
·
生物传感器
·
免疫分析
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Immunotoxicology
·
2019
·
IF 1.734
Penghuan Chang,Ling Huang,Mianqing Huang,Shuhong Tian,Zhaoxin Yang
Although T-cell-dependent antibody response (TDAR) assays with keyhole limpet hemocyanin (KLH) as specific antigen have many advantages, most experiments produce qualitative results based on antibody titers. It was hypothesized that if experimental conditions (like antigen coating concentration, serum dilution, and detecting [here, horseradish peroxidase-goat anti-mouse IgG] antibody dilution) could be optimized, the resulting measured value (here, optical density) could be used to directly analyze and evaluate the experimental results. This means specifically that the assay OD values could be used for approximate quantitative statistical analysis; it does not require a further conversion of the data into qualitative forms or require obtaining further titer data from additional experiments. As such, the use of this “improved” assay would: greatly reduce the complexity of experimental operations; improve overall sensitivity and practicality of traditional TDAR assays; and, allow for direct assessing of any immunosuppression caused by a test drug in a host. Here, KLH-immunized serum was obtained from BALB/c mice, and the means to detect serum anti-KLH antibodies by an indirect ELISA were optimized. The results indicated that in this system, the optimal KLH coating concentration was 80 μg/ml, the optimal dilution range of the serum (at immunization dose of 5 mg KLH/kg) was 1:200–1:800, and the optimal dilution of HRP-goat anti-mouse IgG antibody was 1:16,000. Methodology verification was performed and a regression model of y = 144.16x + 0.9815 (R2 = 0.9571, indicating very good linearity) was obtained. Intragroup precision was 7.51–9.40%; the intergroup coefficient of variation was 9.83–14.22%. The lower limit of detection was 0.1385. The present results showed this indirect ELISA exhibited very good linearity, accuracy, and precision.
研究领域:
免疫学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Cuixia Guo,Ziling Ye,Yonghong He,Zeyu Huang,Hongjian Zhang,Feng Huang
In response to the urgent requirement for high-throughput multiplexed detection, a novel dual-encoded suspension array (SA) based on thickness and digital fluorescent peak is proposed, along with the corresponding optical system. The SAs are prepared by thickness encoded micro-glass chips (MGCs) with electrostatically adsorbed quantum dots (QDs). Using the thickness characteristics of microcarrier itself and digital fluorescence peak (DFP) as the encoded signals ensure the high encoding stability with simple fabrication process at a low cost. A spectral-domain phase and fluorescence spectroscopy microscopy (SDPFSM) system is designed for the first time to decode the thickness information and DFP with ultrahigh accuracy. The high selectivity and sensitivity of the dual-encoded SAs are verified by the multiplexed immunoassays . The quantitative detection of anti-human IgG, anti-mouse IgG, and anti-rabbit IgG was realized with the limits of detection (LOD) of 0.19, 0.35 and 0.31 pM/mL and the sensitivity of 713, 470 and 456 a.u./(pM/mL), respectively. The coding number increases by a factor of 458 compared with the traditional fluorescence coding method due to the additional use of the thickness information of the MGCs itself as a coding channel. The aforementioned properties demonstrate that the dual-encoded SA and the corresponding decoding platform are promising and robust in multiplexed detection.
研究领域:
生物传感器
·
分析化学
·
生物医学工程
·
光学工程
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 3
·855-862
·
IF 3.719
Yan Fufeng,Zhang Yuanchang,Zhang Shuai,Zhao Jihong,Liu Shunli,He Linghao,Feng Xiaozhong,Zhang Hongzhong,Zhang Zhihong
We report on an efficient method for the production carboxy-modified graphene (carboxy-GR), and its application to a highly sensitive immunoassay for the livestock feed additive clenbuterol. Octadecanoic acid is shown to self-assemble on carboxy-GR via intermolecular interaction. Goat anti-mouse IgG was conjugated to 1-octadecanethiol-modified carboxy-GR and then deposited by self-assembly on a C18-modified gold surface. The resulting sensor surface was used to quantify clenbuterol by both surface plasmon resonance and electrochemical impedance spectroscopy. The assay works in the 0.01 ng∙mL −1 to 10 ng∙mL −1 concentration range and the lower detection limit is 6.57 pg∙mL −1 . It is concluded that this fairly simple method can be extended to immunoassays for other food additives.
Principle of sensor system and immunoassay. (i) Binding of ACG–IgG with the alkyl chain of 1-octadecanethiol through intermolecular force onto the gold substrate; (ii) absorbance of Ab CLB onto the surface of ACG-IgG; and (iii) detection of CLB using an immunoassay based on Ab CLB –ACG–IgG.
研究领域:
材料科学
·
分析化学
·
食品安全
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）