JOURNAL OF PHYSICAL CHEMISTRY B
·
2015
·
IF 3.302
Mei Huang,Shuai He,Wenhong Liu,Yunjin Yao,Shiding Miao
Studies on the configuration of dye molecules are of great importance in revealing origins of the electronic bands as well as understanding their transitions. In this work, we utilized dye molecules named Nile blue A, which are a type of oxazine dyes, to study the molecular configurations when they are transferred from solutions to a solid surface. The Langmuir–Blodgett (LB) technique was employed to construct such an interface where the interaction between the dye molecules and solid supports can be pursued. Hybrid films were prepared via the LB depositions, and the dye molecules were assembled on the elementary clay sheets (laponite, saponite). The configuration of Nb reflected by the molecular orientation, packing density, phase behavior, and variances of the surface tension has been derived. The ex situ spectroscopy characterizations such as UV–vis absorption, fluorescence emission, and excitation spectra were carried out on these LB films to reveal the fact that the adsorbed Nb molecules are mainly assembled in two types of configurations. Adsorbed state I was found to be achieved at high concentrations (1–10 ppm) of clay dispersions and low surface pressure (∼5 mN/m). In this state the anionic oxazine rings of Nb are adsorbed on clay sheets sharing a large lift-off area. This configuration gives allowable fluorescence (λ = 550 nm). Lower clay concentration (<1 ppm) and high surface pressure (10–30 mN/m) yield the adsorbed state II in which the oxazine chromophores were arranged in a side-by-side style, and the dye molecules stand perpendicularly to the clay sheets. This conformation exhibits no photoluminescence.
研究领域:
物理化学
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材料科学
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光谱学
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DOI
PubMed
高影响因子
ANALYST
·
2014
·Vol. 139
·期 22
·5858-5867
·
IF 3.906
Yizhong Shen,Shaopu Liu,Ling Kong,Xuanping Tan,Youqiu He,Jidong Yang
Although various strategies have been reported for double-stranded DNA (DNA) detection, development of a time-saving, specific, and regeneratable fluorescence sensing platform still remains a desired goal. In this study, we proposed a new DNA detection method that relies on an “off-on” switch of a regenerated fluorescence biosensor based on an electron transfer mechanism from glutathione (GSH)-capped CdTe quantum dots (QDs) to nile blue (NB). Initially, the high fluorescence of GSH-capped CdTe QDs could be effectively quenched by NB due to the binding of NB to the GSH on the surface of the QDs and the electron transfer from the photoexcited GSH-capped CdTe QDs to NB. Then, the high affinity of DNA to NB enabled the NB to be dissociated from the surface of GSH-capped CdTe QDs to form a more stable complex with DNA and suppress the electron transfer process between GSH-capped CdTe QDs and NB, thereby restoring the fluorescence of NB surface modified GSH-capped CdTe QDs (QDs–NB). In addition, we have testified the regenerability of the proposed DNA senor. The corresponding result shows that this DNA sensor is stable for two reuses. This fluorescence “off-on” signal was sensitive to the concentration of DNA in the range from 0.0092 to 25.0 μg mL−1 with a good correlation coefficient of 0.9989, and the detection limit (3σ/S) was 2.78 ng mL−1. To further investigate for perfect analysis performance, the developed biosensor was applied for the determination of DNA in human fresh serum samples with satisfactory results.
研究领域:
生物化学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2023
·
IF 3.8
Chenglu Zhang,Shiru Nie,Libo Shang,Chang Liu,Yanpeng Zhang,Yang Zhang,Jinghao Guo
A dual responsive and “turn-on” fluorescent probe NapL with large Stokes shift (115 nm) and large fluorescence emission (585 nm) was designed, synthesized and investigated. NapL has high selective and suitable sensitive (LOD = 82.6 nM) towards Cu 2+ over a wide pH range (7.4–12.1) through a fluorescence “turn-on” mechanism and with rapid response to Cu 2+ (30 s) in complex environments. Furthermore, NapL is sensitive toward pH changes within range (7.4–2.1) with obvious "turn - on" effect (about 37- fold). The sensitive detection characteristics of Cu 2+ can be ascribed to the coordination performance of N and O atoms to Cu 2+ , whereas pH responsiveness is ascribed to the protonation of imino group. NapL has been made into convenient and low-cost pH test strip for detecting pH range (7.4–2.1), and it has also been successfully used for the detection of Cu 2+ in MCF-7 breast cancer cells under neutral conditions.
研究领域:
分析化学
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生物医学
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材料科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2022
·
IF 5.304
Zhichen Deng,Jiahua Zhang,Zhengquan Su,Yan Bai,Jincan He
Herein, a multi-spectroscopic assay based on a derivatization reaction was proposed for phosphate detection. In this assay, the phosphate with weak spectral signals was transformed to a large ion-association complex via a derivatization reaction. The operation procedure of the assay was easily performed by physically mixing sulfuric acid, derivatization reagent, phosphate (or sample solution), and dye probe (Nile blue A) for 10 min. Considerable changes in three types of spectral signals, i.e., resonance Rayleigh scattering (RRS), fluorescence, and ultraviolet-visible absorption, could be achieved. Under certain conditions, good linear relationship was obtained between each of the three signal variations and the phosphate concentration. The assay shows good selectivity and high sensitivity with limits of detection (LOD) of 4.8, 4.9 and 5.2 ng/mL for the RRS method, fluorophotometry and spectrophotometry, respectively. The multi-spectroscopic assay was applied to detect phosphate in real food samples, achieving recoveries of 93.3 %–109.6 % and relative standard divisions of 1.8 %–7.1 %. Owing to its speediness, easy operation, high sensitivity, good selectivity, self-comparison function, the multi-spectroscopic assay exhibits great potential for determination of phosphate in complex samples and provides an instructive strategy for detecting analytes with no or weak spectral signal.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2015
·Vol. 162
·期 4
·H229
·
IF 3.266
Mei Huang,Xiaoli Mao,Dechao Chen,Shulian He,Shuai He,Wenhong Liu,Jinzhang Xu,Shiding Miao
A new type of Nile blue (Nb) derivative molecules terminated with long-chain alkyl (termed as Nb18) has been synthesized from precursors of barium stearate and 3-amino-7-(diethylamino)-1,2-benzophenoxazin sulfate. Optical characterizations including UV-Vis absorption, fluorescence emission, excitation spectra and the infrared spectroscopy reveal a blueshift in the band emission of Nb18 as compared to the Nb molecule, and this shift was found to be reasoned by incorporation of long-chain alkyl groups and replacement of SO42−. The amphiphilic Nb18 molecules were found to form stable Langmuir monolayers over aqueous solutions, and this allows one to study the kinetics of Nb18 formation by means of surficial chemistry. The synthetic reaction was demonstrated to have first-order kinetics with a rate constant of ∼0.0033 s−1. Using different techniques (Langmuir-Blodgett and Langmuir-Schaefer) monolayers of Nb18 were deposited on an electrode where the amphiphilic molecules were differently oriented. Investigation of these films undertaken to confirm some of the previous electrochemical observations indicated that the electro-catalytic activity stems from the cationic rings of aromatic oxazine regarding to the oxidation of dihydronicotinamide adenine dinucleotide (NADH).
研究领域:
有机化学
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表面化学
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电化学
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DOI
高影响因子
NANO
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2018
·
IF 1.1
Chang Liu,Qianqian Su,Li Li,Jie Sun,Jian Dong,Weiping Qian
In this work, we designed a simple substrate-immersed solvothermal route for the one-step synthesis of novel ordered SiO 2 /Ag arrays, employing SiO 2 colloidal crystals as templates and alcohol as reducing agent. The Ag nanoparticles were uniformly deposited in situ onto SiO 2 colloidal crystals, which exhibited high surface enhanced Raman spectroscopy (SERS) activity and uniform SERS intensity. It was found that ordered SiO 2 /Ag arrays could rapidly scavenge the absorbed-Nile blue A (NBA) molecules from the surfaces with the assistance of H 2 O 2 , while the SERS signals of NBA decreased sharply and almost completely disappeared within four minutes. This can be attributed to the superior catalytic activity of Ag nanoparticles. After five times of re-immersion and re-absorbing process of NBA, the substrates could still keep ∼ 74.8% SERS intensity versus the original. The high activity and durability of the as-prepared SiO 2 /Ag SERS substrate endow them as a promising candidate for trace detection.
研究领域:
材料科学
·
化学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Shipeng Jiang,Siyu Han,Xinyi Wang,Yueran Ma,Yue Chen,Mingyang Sun,Peiran Meng,Shaokai Du,Yue Sun
Rapid and portable lipopolysaccharide (LPS) detection is of great clinical significance. In this study, a paper-based (PB) sensor for rapid LPS detection was developed based on a smartphone and ultrafast atom transfer radical polymerization (UATRP). First, the filter paper (FB) was modified with chitosan (CS) and glutaraldehyde (GA). LPS was modified onto FB using an amino aptamer (Apt), and this cis-diol site from LPS was selectively cross-linked with the boronic acid group of the initiator 4-(bromomethyl) phenylboronic acid (BPA). Then, UATRP was performed using carbon quantum dots (CQDs) as the catalyst and methacrolein (MLA) as the functional monomer, to generate aldehyde-rich polymer brushes on filter paper. After the efficient covalent conjugation of abundant aldehyde groups with chlorinated Nile blue (CNB), the paper-based sensor was successfully prepared. RGB values were obtained by a smartphone application (App) for analysis. Under the optimized experimental conditions, the developed sensor demonstrated good performance compared to other PB-based sensors. It achieved a lower detection limit (LOD) of 22.86 pg mL−1 (S/N = 3) and exhibited a wider linear detection range (1.0 × 102 to 1.0 × 108 pg mL−1). Importantly, the sensor exhibited both high selectivity and remarkable operational stability during LPS detection. These results not only validate the sensor's analytical reliability but also provide a strong foundation for clinical applications and broader biomedical uses.
研究领域:
生物医学工程
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分析化学
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临床诊断学
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纳米生物技术
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转化医学
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产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Hui Jin,Rijun Gui,Yanfeng Wang,Jie Sun
In this article, a facile and green synthesis of carbon dots (CDs) was developed by using natural carrot as new carbon source. After direct hydrothermal carbonization for 5 h at 180 °C, CDs were prepared facilely. Then, CDs were conjugated with polyethyleneimine (PEI) and Nile Blue (NB) chloride to produce CDs/PEI/NB nanocomposites under electrostatic interactions . Upon excitation at 800 nm, two-photon fluorescence (TPF) of the nanocomposites was observed, with TPF peaks of CDs at 415 nm and NB at 675 nm. The addition of Cu 2+ could lead to TPF quenching of CDs via inner filter effect, but hardly any impacted on TPF of NB. Afterward, the added S 2- combined with Cu 2+ to form stable species that caused the separation of Cu 2+ from CDs surface and the TPF recovery of CDs, with negligible effects on TPF of NB. Herein, a new CDs-based ratiometric TPF turn-on probe of S 2- was developed and showed a good linear relationship ( R 2 =0.9933) between ratiometric TPF intensity ( I 415 / I 675 ) and S 2- concentration (0.1–8.0 μM), with a low detection limit of 0.06 μM. This probe was highly selective and sensitive toward S 2- over potential interferences in real biological fluids, with high detection recoveries.
研究领域:
分析化学
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材料科学
·
生物化学
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Fugang Xu,Shi Xie,Hui Xu,Xing Chen,Han Yu,Li Wang
In this study, a new plasmonic hybrid AgNSs@PANI/3D-CF composed of interlaced silver nanosheets (AgNSs) grown on polyaniline (PANI) nanobars decorated three dimensional macroporous carbon foam (3D-CF) was prepared for molecule sensing by surface enhanced Raman scattering (SERS). The morphology, component, adsorption ability and SERS activity of the hybrid were characterized by SEM, EDS, FTIR, XRD, UV–vis absorption spectrum, and Raman spectroscopy. Controlling the silver growth time and introduction of succinic acid are vital to obtain the interlaced silver nanosheets fully covered 3D scaffold. The porous structure and large surface area of the hybrid bring it a high adsorption ability to dye molecules. And interlaced AgNSs endow the hybrid with dense hot-spots for highly efficient SERS detection. The lowest SERS detectable concentration of 4-mercaptobenzoic acid (4-MBA), Nile blue (NB) and Methylene blue (MB) on AgNSs@PANI/3D-CF was 0.1 nM, 0.1 nM and 10 nM, respectively. A good result was also achieved for NB detection in real water sample. The proposed hybrid with unique structure, high Raman enhancement ability and good reproducibility may find promising applications in environment monitoring, optical sensing and so on.
研究领域:
材料科学
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纳米技术
·
分析化学
·
环境监测
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Xiao-Fang Hou,Shu Zhang,Xiao Chen,Hari Krishna Bisoyi,Tianchi Xu,Jiang Liu,Dongzhong Chen,Xu-Man Chen,Quan Li
Artificial supramolecular light-harvesting systems have expanded various properties on photoluminescence, enabling promising applications on cell imaging, especially for imaging in organelles. Supramolecular light-harvesting systems have been used for imaging in some organelles such as lysosome, Golgi apparatus, and mitochondrion, but developing a supramolecular light-harvesting platform for imaging two organelles synchronously still remains a great challenge. Here, we report a series of lower-rim dodecyl-modified sulfonato-calix[4]arene-mediated supramolecular light-harvesting platforms for efficient light-harvesting from three naphthalene diphenylvinylpyridiniums containing acceptors, Nile Red, and Nile Blue. All of the constructed supramolecular light-harvesting systems possess high light-harvesting efficiency. Furthermore, when the two acceptors are loaded simultaneously in a single light-harvesting donor system for imaging in human prostate cancer cells, organelle imaging in lysosome and Golgi apparatus can be realized at the same time with distinctive wavelength emission. Nile Red receives the light-harvesting energy from the donors, reaching orange emissions (625 nm) in lysosome while Nile Blue shows a near-infrared light-harvesting emission at 675 nm in Golgi apparatus in the same cells. Thus, the light harvesting system provides a pathway for synchronously efficient cell imaging in two distinct organelles with a single type of photoluminescent supramolecular nanoparticles.
研究领域:
生物物理学
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分子生物学
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纳米技术
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Nuo Duan,Yuting Chang,Wenhui Lv,Changxin Li,Chunxia Lu,Zhouping Wang,Shijia Wu
Herein, a SiO 2 @Ag NPs core/shell nanoparticles were synthesized to fabricate a surface-enhanced Raman spectroscopy (SERS) sensor for the simultaneous determination of histamine (HIS) and tyramine (TYR) based on specific aptamer recognition and ratiometric strategy. SiO 2 @Ag NPs with 4-thiosaminophenol (4-ATP) and Nile blue A (NBA) molecules were used as an internal standard (IS) and labeled with aptamers corresponding to HIS and TYR, respectively. Raman reporter molecules ROX and Cy5 labeled complementary DNA (cDNA) were then hybridized with aptamers to form rigid double-stranded DNA. After the HIS and TYR were captured by their aptamers, resulting in the dissociation of cDNA and separated from the SERS substrate. Therefore, the SERS signal intensity at 1503 cm −1 of ROX and 1358 cm −1 of Cy5 tagged on the terminal of cDNA decreased with the concentration of HIS and TYR increasing, while the SERS signal intensity at 1079 cm −1 of 4-APT and 592 cm −1 of NBA on the substrate remain stable. Thus, the concentrations of HIS and TYR can be determined by the I 1503 /I 1079 and I 1358 /I 592 values, respectively. This sensing strategy achieves a lower detection limit of 0.2 ng/mL for HIS and 0.05 ng/mL for TYR, respectively, demonstrating promising applications in sensitive detection of BAs in animal-derived foodstuff.
研究领域:
分析化学
·
食品科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Liqi Wang,Hairong Zhang,Ziyue Li,Jiayao Qu,Kaiwen Xing,Minghao Wang,Cuiping Han,Zeguo Qiu,Dianyu Yu
In recent years, photoelectrochemical (PEC) biosensors have shown great application prospects in biological analysis and food detection. In this work, we developed and tested a new PEC biosensor. Three-dimensional graphene oxide (3DGO) was generated and deposited on an indium tin oxides (ITO) electrode. The photoelectrically active poly[Nile blue] (PNb) was formed on this electrode by electropolymerization, and glycerol dehydrogenase (GDH) was immobilised to generate the GDH-PNb-3DGO-ITO biosensor. This newly developed PEC biosensor exhibited excellent photoelectric response to lipase activity under light conditions, with a detection range of 0.1–6 U/mL and a lower detection limit of 0.069 U/mL. When the GDH-PNb-3DGO-ITO sensor was used for PEC detection of lipase activity by the standard addition recovery method, the recovery rate of the standard addition was 98.68–105.41%, and the relative standard deviation (RSD) was 2.1–3.5%, with good storage stability and repeatability. Therefore, this work provides new ideas for the design and preparation of PEC biosensors and introduces a new detection method for monitoring lipase activity.
研究领域:
生物传感器
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分析化学
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生物技术
·
材料科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）