JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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2018
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IF 0.827
Huihui Wu,Hanying Jin,Guangxin Yuan,Jacques Crommen,Jing Zhang,Qiqin Wang
Urea and allantoin are two important additives in cosmetic products for the treatment of dry skin as well as for skin protection and regeneration. In this research, a highly hydrophilic monolith prepared by co-polymerization of N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine (SPDA) and N,N’-methylenebisacrylamide (MBA) was first exploited for the simultaneous quantification of urea and allantoin in cosmetic products by nano-HPLC in the HILIC mode. The whole separation process could be completed within six minutes using isocratic elution. Due to the extremely high hydrophilicity of this monolithic column, a relatively low content (80%) of ACN in the mobile phase was required to achieve satisfactory separation, and therefore the solubility problems for polar compounds was also avoided in comparison with previously reported results on the commercial columns. Method validation was also accomplished with respect to linearity, reproducibility, selectivity, recovery, stability, LOD and LOQ. The recoveries of urea and allantoin from spiked cosmetic samples were 95.6%-100% and 94.1%-99.9%, respectively. Validation results indicate that this HILIC method based on the miniaturized poly(SPDA-co-MBA) monolith can be applied successfully to the fast and efficient determination of urea and allantoin in cosmetic samples. The miniaturized column format, the low sample consumption (20 nL), and excellent separation efficiency could make this highly hydrophilic monolith an attractive choice for the detection of trace polar analytes from complex samples in future.
研究领域:
分析化学
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化妆品科学
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DOI
高影响因子
MICROCHIMICA ACTA
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2013
·Vol. 180
·期 15
·1453-1460
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IF 3.434
Zhong Xianwen,Deng Fang,Wang Yuehua,Luo Xubiao
We describe a molecularly imprinted polymer (MIP) for the solid-phase extraction of the skin protectant allantoin. The MIP was deposited on the surface of monodisperse silica microspheres possessing acroyl groups on the surface (MH-SiO 2 ). The resulting MIP microspheres (MH-SiO 2 @MIP) showed a 3.4-fold higher adsorption capacity and a 1.9-fold better selectivity for allantoin than the respective non-imprinted polymer (MH-SiO 2 @NIP). The monolayer adsorption capacities of the MH-SiO 2 @MIP and the MH-SiO 2 @NIP were calculated with the help of the Langmuir model and found to be 6.8 and 1.9 mg•g −1 , respectively. Adsorption kinetics fit a pseudo-second order rate mechanism, with an initial adsorption rate of 1.44 for the MH-SiO 2 @MIP, and of 0.07 mg•g −1 •min −1 for the MH-SiO 2 @NIP. The material can be regenerated, and its adsorption capacity for allantoin remains stable for at least five regeneration cycles. It was successfully used as a sorbent for the selective solid-phase extraction of allantoin from Rhizoma dioscoreae .
A molecularly imprinted polymer for the selective separation of allantoin was developed. It was successfully used as a sorbent for the selective solid-phase extraction of allantoin from Rhizoma dioscoreae .
研究领域:
药物分析
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材料科学
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天然产物化学
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分离科学
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高分子化学
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高影响因子
JOURNAL OF PLANT PHYSIOLOGY
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2023
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IF 4.3
Gang Wang,Jiali Li,Jing Ji,Lishuang Zhang,Bowen Li,Jiaqi Zhang,Xinya Wang,Wenju Song,Chunfeng Guan
Environmental pollution by 2, 4 dichlorophenol (2, 4-DCP) has become a widespread concern due to its detrimental influence on human and natural ecosystem. With the increasing accumulation of 2, 4-DCP in soil, it is of great significance to explore some appropriate approaches for enhancing plant tolerance to 2, 4-DCP stress. In the current study, a strain resistant to 2, 4-DCP was obtained from the tall fescue rhizosphere soil and named as Pseudomonas sp. JIT1. The strain JIT1 exhibited several remarkable plant growth-promoting traits, including the production of IAA, fixation of biological nitrogen and solubilization of phosphate. The inoculation of strain JIT1 significantly increased biomass, photosynthesis, antioxidant levels, chlorophyll contents and the osmotic substance contents in rice seedlings exposed to 2, 4-DCP. Meanwhile, inoculation of strain JIT1 also enhanced activities of soil alkaline phosphatase, urease, sucrase and cellulase. Moreover, under 2, 4-DCP stress, the content of allantoin in seedlings significantly increased and the pretreatment of exogenous allantoin noticeably ameliorated the negative effects caused by 2, 4-DCP stress in rice seedlings. Interesting, allantoin treatment also enhanced phosphate solubilization properties of strain JIT1. The chlorophyll contents, photosynthesis and osmotic substance further increased by combination use of strain JIT1 and allantoin, which improved the growth of seedlings, most likely to be attributed to the synergistic or additive effect between allantoin and strain JIT1. The results of this study highlight the important roles of combined use of strain JIT1 and allantoin for improving the tolerance of rice to 2, 4-DCP to stress.
研究领域:
环境科学
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农学
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微生物学
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植物生理学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Haichao Zhu,Hoi tong Ao,Yuli Fu,Chenming Zou,Ziyan Chen,Zhu Jin,Huawei Zhou,Bixi Sun,Shengrong Guo
Wound healing requires diverse functionalities in dressings, and conventional materials often fall short in water absorption and moisture regulation. Natural sodium alginate is popular in wound dressings due to its excellent film-forming ability, biocompatibility, ionic crosslinking, and pH responsiveness. However, it has limitations in physical stability and solubility in aqueous environments. This study enhanced alginate dressings by incorporating allantoin and treating with calcium chloride and citric acid to improve physicochemical properties and mechanical performance. Treatments for S2 to S5 prevented dissociation and maintained integrity, with suitable water absorption (363 %–442 %) and water vapor transmission rates (612.53–715.39 g × m 2 × day −1 ). The treatments also improved tensile strength (44.90–55.19 MPa). S2 had the highest migration ratio (52.71 %) of L929 cells and wound healing rates for mice skin (86.6 %), indicating that calcium chloride treatment is beneficial. All dressings (S1 to S5) exhibited low cytotoxicity against L929 cells and low hemolysis ratios, indicating good biocompatibility. Higher allantoin content improved wound healing efficacy. This study provides valuable insights for the design and development of alginate dressings in wound repair, expanding allantoin's application in wound healing.
研究领域:
材料科学
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生物医学工程
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药剂学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Haojiang Xie,Qiao Bai,Fankai Kong,Yang Li,Xiaoying Zha,Lingqin Zhang,Yiming Zhao,Shasha Gao,Ping Li,Qifeng Jiang
In clinical application, it's highly desirable for developing bio-functionalized cutaneous scaffold with transparent features for convenient observation, excellent biocompatibility, and high efficiency for promoting wound repair. Herein, allantoin-functionalized composite hydrogel was developed by coupling silk fibroin (SF) and sodium alginate (SA) for treatment of cutaneous wounds. The prepared allantoin-functionalized SF-SA composite scaffolds (AFAS) exhibited excellent mechanical properties, especially featured by similar ultimate tensile strength (UTS) and elongation at breaking to human skin. Besides, the solvent-casting method guaranteed the AFAS to obtain highly transparent properties with sufficient moisture permeability and excellent adhesion in wet state. In vitro cellular experiments demonstrated excellent biocompatibility of the scaffold that attachment and proliferation of NIH-3T3 fibroblast cells was promoted in the presence of AFAS. Furthermore, the scaffolds exhibited efficient hemostatic property, based on rat hepatic hemorrhage model. In a cutaneous excisional mouse wound model, the AFAS significantly improved the wound closure rate, compared with pure SF-SA scaffolds and blank control. Moreover, the histomorphological assessments showed that AFAS facilitated the integrity of skin and wound healing process by enhancing collagen deposition, re-epithelialization and vascularization at wound site. The results demonstrate that the novel allantoin-functionalized SF/SA transparent hydrogel has great potential for clinical treatment of cutaneous wound.
研究领域:
生物材料
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组织工程
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伤口愈合
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生物医学工程
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DOI
PubMed
高影响因子
Biomaterials Advances
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2024
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IF 5.5
Manjila Adhikari,Bianza Moise Bakadia,Li Wang,Ying Li,Zhijun Shi,Guang Yang
Effective treatment of infected wounds remains a challenge due to the rise of antibiotic-resistant microorganisms. The development of advanced materials with strong antimicrobial properties is necessary to address this issue. In this study, a unique composite of electrically modified bacterial cellulose (EBC) with allantoin (ABC) and zein was developed by dipping diffusion method. Morphological structural analysis revealed a uniform distribution of zein and aligned fibers, confirming the synthesis of the ABC-Zein composite. The formation of ABC-Zein was further confirmed by attenuated total reflection-Fourier transform infrared (ATR-FTIR), which displayed additional peaks corresponding to EBC, indicating the incorporation of zein into ABC. X-ray diffraction (XRD) analysis of ABC-Zein demonstrated a similar crystalline structure with EBC. The ABC-Zein showed mechanical integrity (tensile strength: 1.15 ± 0.21 MPa), thermal stability (degradation temperature: 290 °C), porous structure (porosity: 40.23 ± 0.21 %), and hydrophilic (water contact angle: 53.3 ± 5.3°) properties. Furthermore, the antimicrobial agent terpinen-4-ol (T4O), derived from tea tree oil, was incorporated into the ABC-Zein composite. Biological studies confirmed the antimicrobial efficacy ( Staphylococcus aureus inhibition: 88.5 ± 7.19 %) and biocompatible (cell viability: 84.95 ± 5.6 %, hemolysis: 4.479 ± 0.39 %) nature of the T4O-ABC-Zein composite. The combined effects of the aligned fiber structure, zein protein, and antimicrobial T4O significantly enhanced infected wound healing by day 7, promoting inflammatory response, granular tissue formation, cell proliferation, and angiogenesis. By day 14, T4O-ABC-Zein facilitated complete wound healing, with reepithelization, collagen I deposition, and downregulation of CD 31, Ki67, and α-SMA. Overall, the innovative T4O-ABC-Zein composite, with an aligned fiber structure, improved biocompatibility, and antimicrobial properties, holds significant potential for the treatment of infected wounds.
研究领域:
生物材料学
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医学
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微生物学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Yue Kong,Xu Fan,Rongkai Wu,Shibin Nie,Chao Liu,Xiaoyong Liu,Guangyi Zhang,Bihe Yuan
The domain of smart textiles represents a widely researched area, showcasing promising prospects. Herein, a multifunctional smart textile (CF@PM-AA) has been designed and fabricated, amalgamating flame retardancy, electrical conductivity, and hydrophobicity into a unified entity. The flame retardant, synthesized through the reaction between amino trimethylene phosphonic acid and allantoin, was impregnated into cotton fabric through a facile impregnation method. Through surface modification with polydimethylsiloxane and multi-walled carbon nanotubes, the obtained CF@PM-AA acquires exceptional hydrophobic and acid-base environment durability. Significantly, the electrical conductivity of CF@PM-AA is greatly enhanced, with a reduced resistance down from 15.9 GΩ to 13 MΩ. Based on their electrical characteristics, the copper sheet and CF@PM-AA function as the electrode and friction layers, respectively, in the construction of the triboelectric nanogenerator. This configuration produces a voltage output of up to 125.6 V at a frequency of 4 Hz. Given its outstanding flame retardancy, hydrophobicity, and electrical performance, CF@PM-AA can be integrated into firefighting suits to establish a fire-alarm system with remote signal transmission function, enhancing personnel safety. Simultaneously, the CF@PM-AA holds promise for diverse applications in wearable flame-retardant and fire-alarm systems, opening new avenues for smart textile utilization in the Internet of Things domain.
研究领域:
材料科学
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智能纺织品
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纳米技术
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2024
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IF 3.8
Liang Lai,Mengyao Zhang,Tong Li,Jiahuan Qu,Dongsheng Xu,Pei Yu,Zhengjin Jiang
In this study, a novel piperidinium-sulfonate based zwitterionic hydrophilic monolith was prepared through thermally initiated co -polymerization of a piperidinium-sulfonate monomer 3-(4-((methacryloyloxy)methyl)-1-methylpiperidin-1-ium-1-yl)propane-1-sulfonate (MAMMPS), and a hydrophilic crosslinker N,N′ -methylenebisacrylamide (MBA) using n -propanol and H 2 O as porogenic system. Satisfactory mechanical and chemical stabilities, good repeatability and high column efficiency (120,000 N/m ) were obtained on the optimal monolith. The resulting poly(MAMMPS- co -MBA) monolith showed a typical HILIC retention behavior over an ACN content range between 5 and 95 %. Furthermore, this column exhibited good separation performance for various polar compounds. Compared to quaternary ammonium-sulfonate based zwitterionic hydrophilic monolith, i.e. poly(N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl)ammonium betaine-co-MBA), the poly(MAMMPS- co -MBA) monolith displayed stronger retention and better selectivity for the tested phenolic and amine compounds at different pH conditions. Finally, this column was applied for the separation of six sulfonamide antibiotics, and the analytical characteristics of the method were evaluated in terms of precision, repeatability, limits of detection (LOD) and quantitation (LOQ). Overall, this study not only developed a novel HILIC monolithic column, but also proved the potential of piperidinium-sulfonate based zwitterionic chemistry as stationary phase, which further increased the structure diversity of zwitterionic HILIC stationary phases.
研究领域:
分析化学
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材料科学
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色谱学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Yizhan Wang,Yunhui Shi,Jiawei Qiu,JiaBao Cheng,Yao Xu,Yongxin Wang
The intercalation of organic molecules is a promising approach to modulate the structure of 2D transition metal borides (MBenes), aiming to enhance charge transport and improve electrochemical performance in energy storage applications. However, key questions remain regarding how organic molecules with diverse functionalities penetrate and align between the MBene layer, as well as the mechanism of charge redistribution during intercalation. Addressing these questions is crucial for guiding the design of Organic-MBene heterostructures. To this end, a comprehensive approach combining theoretical calculations and experimental analyses was employed to explore the self-assembly mechanisms of organic molecules featuring N, O, S and tertiary amine end groups on the MoB-MBene surface. Experimental characterizations confirm that the interaction between MoB and organic compounds depends on the end groups. First principles calculations demonstrate that organic molecules tend to adopt a flat configuration on the MoB surface during molecular assembly. Calculations also reveal that the binding and charge transfer processes from organic molecules to MoB are highly dependent on the specific end groups, consistent with experimental observations. Furthermore, the effect of combining organic molecules with MoB on battery performance was further discussed, offering new insights for advancing the research and development of MBenes in aqueous battery systems.
研究领域:
材料科学
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物理化学
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PubMed
高影响因子
Journal of Environmental Sciences
·
2026
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IF 6.5
Ning Hu,Kaikai Zheng,Weiwei Wei,Xiaowen Fang,Yingqing Guo,Xian Xiao,Yuan Zhao
Distinct root exudate profiles are linked to root morphology in emergent plants. • We observed similar rhizosphere microbial communities despite distinct root exudate profiles. • Root exudates of Z. latifolia exert chemotactic attraction toward denitrifiers such as Pseudomonas and Acinetobacter . • Core metabolites, including phthalic acid and allantoin, can predict shifts in denitrification microorganisms. Emergent plants with different root morphologies show considerable variation in their nitrogen removal capabilities via nitrification and denitrification processes; however, the underlying mechanisms are still unknown. This study investigates how root morphology influences root exudates and rhizosphere microbial communities in two emergent plant species: Zizania latifolia (taprooted) and Pontederia cordata (fibrous-rooted). Root exudates were collected and analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), revealing distinct metabolic profiles between the two species. Z. latifolia primarily secreted benzenoids, while P. cordata exuded higher levels of lipids and lipid-like molecules. Despite these differences, the rhizosphere bacterial and fungal communities of both plants were similar in both structures and potential functions. Interestingly, the differential metabolites identified in Z. latifolia root exudates, especially amino acids (e.g., L-Valine and D-Proline), exhibited significant and positive correlations with denitrifying bacterial genera, such as Pseudomonas and Acinetobacter , suggesting that these metabolites exhibit strong chemotactic attraction toward specific microbial communities. Specifically, the chemotactic response of Pseudomonas sp. and Acinetobacter sp. to phthalic acid, allantoin, and quinic acid confirmed that these metabolites might recruit denitrifying microorganisms through chemotaxis. These findings highlight the complex interactions between root exudates, rhizosphere microbes, and nitrogen cycling in emergent plants, providing insights for optimizing wetland restoration strategies. Download: Download high-res image (351KB) Download: Download full-size image
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
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IF 4
Minyi Yuan,Zheng Zhao,Ji Wu,Sumin Bian,Yuling Liao,Ying Zhou,Huikai Shao,Zhengjin Jiang,Qiqin Wang
To meet the increasing demand for the separation of complex samples, a novel monolithic stationary phase with hydrophobic and hydrophilic functionalities was developed by using 3-(1-(4-vinylbenzyl)-1H-imidazol-3-ium) propane-1-sulfonate (VBIPS) as monomer, pentaerythritol triacrylate (PETA) as crosslinker. The optimized mixed-mode poly(VBIPS- co -PETA) monolith exhibited good mechanical strength, high permeability (K = 0.23 × 10 -14 m 2 in 100% ACN, 0.23 × 10 -14 m 2 in 100% MeOH, and 0.11 × 10 -14 m 2 in 100% H 2 O), and multiple interaction modes, including hydrophilic, hydrophobic, hydrogen, and electrostatic interactions. The highest column efficiency of 83,330 plates/m was achieved for thiourea at a linear velocity of 0.23 mm/s. Systematic evaluations confirmed strong retention and separation of polar substances (e.g., benzoic acids, nucleoside bases, phenols), primarily due to the sulfobetaine-derived hydrophilicity as well as effective separation of nonpolar substances (e.g., aniline, aryl ketones, polyaromatic hydroxy compounds), facilitated by π-π stacking interactions between analytes and benzene rings within the stationary phase. Overall, this zwitterionic imidazolium sulfonate-functionalized monolith successfully combines hydrophobic and hydrophilic groups in a single continuous bed, enabling efficient separation of both polar and nonpolar analytes under low backpressure. This work enriches the library of mixed-mode stationary phases, and broadens the detection scope for complex sample analysis.
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高影响因子
MICROBIOLOGICAL RESEARCH
·
2025
·
IF 6.9
Chen Guo,Yixiang Sun,Hongjun Chen,Guangyao Yin,Yizhi Song
Hyperuricemia, a prevalent metabolic disorder, necessitates novel therapeutic strategies. This study reports the isolation and characterization of Pichia kudriavzevii YS711, a novel strain isolated from the traditional Chinese fermented food “Jiangshui”, exhibiting exceptional uric acid (UA) degradation capabilities. In vitro studies demonstrated that YS711 degraded 31.2% of UA within 24 hours, converting it to ammonium. This end-product is significantly safer than urea, mitigating potential adverse effects associated with urea accumulation. Whole-genome sequencing revealed a complete UA metabolic pathway in YS711, the first such pathway elucidated in the phylum Ascomycota. This pathway encompasses key enzymes, including urate oxidase, allantoinase, allantoicase, ureidoglycolate lyase, and urea amidolyase. The identification of these genes provides a valuable resource for future metabolic engineering efforts to enhance UA degradation. This research underscores the potential of P. kudriavzevii YS711 as a promising probiotic-based therapeutic agent for hyperuricemia prevention and treatment, offering a safer and potentially more effective alternative to existing approaches.
研究领域:
微生物学
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医学
·
基因组学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）