Drug Testing and Analysis
·
2014
·Vol. 6
·期 10
·1020-1027
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IF 2.84
Yajing Lei,Qian Zhang,Lizheng Fang,Muhammad Sajid Hamid Akash,Kanwal Rehman,Zhiming Liu,Weixing Shi,Shuqing Chen
We simultaneously set up two competitive (direct and indirect) enzyme-linked immunosorbent assays (ELISAs) based on the same antibody for estimation of cotinine (COT) in pregnant women especially and population generally exposed to environmental tobacco smoke. The results show that the limits of detection (LODs) for direct competitive ELISA and indirect competitive ELISA were 0.04 μgL -1 and 0.1 μgL -1 , respectively. Direct competitive ELISA was found to be more sensitive than indirect competitive ELISA. Thereafter, we applied our direct competitive ELISA for the detection of COT from urinary samples taken from 450 volunteers from the Zhejiang Province of China. COT was detected in 100% of participants with concentration ranging from LOD to 5358.0 μgL -1 . The GM and 95th percentile concentration of COT in pregnant women were 6.3 μgL -1 and 57.2 μgL -1 , respectively. Males had statistically higher COT concentrations than females ( P < 0.0001), active smokers had statistically higher COT concentrations than non-smokers ( P < 0.0001), whereas, non-pregnant women were found to have higher COT concentration than pregnant women. We conclude that our developed direct competitive ELISA is useful for detecting the COT in urinary concentration of human. The human urinary data obtained in this study indicated that common people generally and pregnant women especially were highly exposed to COT. Further studies are needed to focus on the sources of exposure, potential health effects and risk assessment of exposure to COT. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
分析化学
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环境卫生
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公共卫生
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PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 7
·3038-3044
·
IF 3.288
Xu Wang,Zhiwei Sun,Xianzhu Luo,Kaifeng Wang,Shijuan Zhang,Zhongyin Ji,Yue Gao,Jinmao You
A novel switchable solvent liquid-phase microextraction technique, based on the solidification of floating organic droplets (SS-LPME-SFO), was developed for the pretreatment of aqueous samples. In this process, octanoic acid was selected as the extractant due to its low density, moderate melting point, and ability to switch between hydrophilic and hydrophobic characteristics. This technique has been successfully applied in the enrichment of five polycyclic aromatic hydrocarbon monohydroxy metabolites (OH-PAHs) in urine samples. Furthermore, a HPLC-FLD detection procedure was established to analyze the samples. A series of parameters affecting the extraction were systematically studied. Under the optimal conditions, all analytes showed good linearity within the studied range, and the values of the correlation coefficient (R2) were higher than 0.99. The limits of detection (LODs) and the limits of quantitation (LOQs) were within the range of 0.018–0.076 μg L−1 and 0.059–0.25 μg L−1, respectively. The method also achieved good recoveries, ranging between 88.5% and 102.5%. Overall, the method is highly cost-effective, environmentally-friendly, fast and efficient. This study proposes an efficient extraction method for the analysis of most organic pollutants in complex environmental aqueous phases.
研究领域:
分析化学
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环境科学
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医学检测
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Yibo Yan,Chuixiu Huang,Xiantao Shen
Aristolochic acid (AA) I and AA II, which have been classified as carcinogenic to human and have been proven to be nephrotoxic, are bioactive ingredients of many traditional Chinese medicines (TCMs). Thus, development of an efficient approach for separation and determination of AA I and AA II in biological samples and herbal plants is of significance. Herein, electromembrane extraction (EME) was for the first time used to separate AA I and AA II. It is noted that also for the first time 1-decanol was discovered and used as an efficient SLM solvent for EME of acidic compounds. The proposed EME system was used to extract AA I and AA II from urine samples (recovery≥68%). The approach of EME combined with LC–MS (EME-LC/MS) was evaluated using urine samples. The linear range for AA I and AA II was 10–1000 ng mL −1 (R 2 ≥0.9970), and the limits of detection (LOD, S/N = 3) for AA I and AA II were 2.7 and 2.9 ng mL −1 , respectively. Finally, this EME-LC/MS approach was employed to discover AA I and AA II in the herbal plants. In addition, using standard addition method, AA I in Aristolochicaceae-Liao Asarum (ALA) and Radix Aristolochice (RA) were 0.23 and 2044.13 μg g -1 , and AA II in ALA and RA were 0 and 338.48 μg g -1 , respectively. The repeatability of EME-LC/MS at all cases for both urine samples and herbal plants was below 15% (RSD-value). We believe that EME would be a useful tool to isolate bioactive ingredients of TCMs from complex samples for different purposes.
研究领域:
分析化学
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药物分析
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毒理学
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2017
·Vol. 24
·期 8
·7042-7050
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IF 2.741
Zhang Dai,Zhou Lifang,Lei Yajing,Zhou Zhan,Zhou Jie,Chen Shuqing
Diethylstilbestrol (DES) is used as a kind of animal feed additive and affects people’s health through the food chain. The purpose of this study is to detect the residue level of DES in 576 human urine samples directly. DES-BSA was used to immunize Balb/c mice. The monoclonal antibody was produced by hybridoma that was screened through cell fusion techniques. Finally, we developed the indirect competitive ELISA method to analyze 576 human urine samples from Zhejiang Province, China. The IC 50 of this method was 3.33 ng/mL. The LOD and LOQ were 0.16 and 0.54 ng/mL. Linear range of the standard curve was from LOD to 12.50 ng/mL. There was no cross-reactivity with two kinds of estrogens and two structural analogs with DES. Five hundred seventy-six urine samples were analyzed by the indirect competitive ELISA method, and the detection rate was 98.78%. The mean concentration and geometric mean were 4.70 and 3.50 ng/mL. The indirect competitive ELISA method based on monoclonal antibody was sensitive and reliable for the detection of DES in human urine samples. The results warned us to pay more attention to human health and food safety.
研究领域:
分析化学
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毒理学
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公共卫生
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PubMed
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2019
·Vol. 31
·期 2
·242-251
·
IF 1.963
Luwei Fang,Jinfeng Xie,Liangwen Lin,Minglei Tian,Kyung Ho Row
Introduction Ephedrine is a typical compound found in lots of plant species that is used in several medicines for the treatment of asthma and bronchitis. However, excess amounts are harmful to humans, so it needs to be removed. Objective This study developed a multi-phase extraction (MPE) method with a molecular imprinted polymer (MIP) coated ionic liquid (IL)-based silica (SiO 2 @IL@MIP) to simultaneously extract and separate ephedrine from Pinellia ternata , 10 medicines, and urine samples. Methods IL was immobilized on silica. Subsequently, the IL was combined with the functional monomer, followed by the addition of the crosslinker and template. The resulting sorbent was applied to the MPE, and the extraction, washing and elution solvents were evaluated. Results Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) confirmed the synthesis of SiO 2 @IL@MIP. A maximum adsorption amount of 5.76 mg/g was obtained at 30°C at a neutral pH. In MPE, 10.00 mL of methanol could extract all the ephedrine from Pinellia ternata . The interference was removed by washing with 4.00 mL of water, ethanol, and acetonitrile. Finally, 8.00 mL of methanol/acetic acid (99:1, v / v ) was applied as the elution solvent. The following were extracted: 5.50 μg/g of ephedrine from Pinellia ternata , 0.00–46.50 μg/g from the 10 herbal medicines, and 68.70–102.80 μg/mL in the urine samples. Conclusion The proposed method was applied successfully to the simultaneously extraction and separation of ephedrine from plants and medicines. These results are expected to provide important data for the development of new methods for the separation and purification of bioactive compounds.
研究领域:
分析化学
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药学
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植物化学
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分离科学
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DOI
PubMed
高影响因子
Nanoscale
·
2022
·Vol. 15
·期 4
·1806-1812
·
IF 8.307
Ruijia Huang,Ying Xu,Jihui Du,Qiong Guan,Xiaoqing Cai,Feng Li,Jidong Wang,Wenwen Chen
Copper is an essential element in the human body, participating in various physiological activities in the bodies of organisms. However, an excessive load of Cu2+ is associated with several neurodegenerative diseases and prion diseases, also identified as a symptom of Wilson's disease (WD). A straightforward, rapid, sensitive, and specific copper sensor is highly required but remains a challenge. In this study, guided by the simulation, we developed a chemical sensor using a cascade signal amplification strategy based on the Cu-catalyzed click reaction, combined with a fluorescence-enhanced substrate with gold nanorods coupled with silver nanoislands. The sensor can selectively detect Cu2+ as low as 3.87 nM within 10 min. We have demonstrated that this method can be directly employed for WD diagnosis in urine samples. In addition, using antibiotic susceptibility testing (AST) as an example, we verify whether this assay can be adapted to other targets where Cu is designed as an indirect indicator.
研究领域:
分析化学
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生物传感器
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医学诊断
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Jiajia Yang,Yun Li,Jincheng Wang,Xiaoli Sun,Rong Cao,Hao Sun,Chaonan Huang,Jiping Chen
The bisphenol A (BPA) imprinted polymer microspheres were prepared by simple Pickering emulsion polymerization. Compared to traditional bulk polymerization, both high yields of polymer and good control of particle sizes were achieved. The characterization results of scanning electron microscopy and nitrogen adsorption–desorption measurements showed that the obtained molecularly imprinted polymer microsphere (MIPMS) particles possessed regular spherical shape, narrow diameter distribution (30–60 μm), a specific surface area ( S BET ) of 281.26 m 2 g −1 and a total pore volume ( V t ) of 0.459 cm 3 g −1 . Good specific adsorption capacity for BPA was obtained in the sorption experiment and good class selectivity for BPA and its seven structural analogs (bisphenol F, bisphenol B, bisphenol E, bisphenol AF, bisphenol S, bisphenol AP and bisphenol Z) was demonstrated by the chromatographic evaluation experiment. The MIPMS as solid-phase extraction (SPE) packing material was then evaluated for extraction and clean-up of these bisphenols (BPs) from human urine samples. An accurate and sensitive analytical method based on the MIPMS-SPE coupled with HPLC-DAD has been successfully established for simultaneous determination of eight BPs from human urine samples with detection limits of 1.2–2.2 ng mL −1 . The recoveries of BPs for urine samples at two spiking levels (100 and 500 ng mL −1 for each BP) were in the range of 81.3–106.7% with RSD values below 8.3%.
研究领域:
分析化学
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环境科学
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医学检测
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Dan-Ya Lyu,Cheng-Xiong Yang,Xiu-Ping Yan
Polymer monolith microextraction (PMME) based on capillary monolithic column is an effective and useful technique to preconcentrate trace analytes from environmental and biological samples. Here, we report the fabrication of a novel aluminum terephthalate metal-organic framework (MIL-53(Al)) incorporated capillary monolithic column via in situ polymerization for the PMME of non-steroidal anti-inflammatory drugs (NSAIDs) (ketoprofen, fenbufen and ibuprofen) in water and urine samples. The fabricated MIL-53(Al) incorporated monolith was characterized by X-ray powder diffractometry, scanning electron microscopy, Fourier transform infrared spectrometry, and nitrogen adsorption experiment. The MIL-53(Al) incorporated monolith gave larger surface area than the neat polymer monolith. A 2-cm long MIL-53(Al) incorporated capillary monolith was applied for PMME coupled with high-performance liquid chromatography for the determination of the NSAIDs. Potential factors affecting the PMME were studied in detail. Under the optimized conditions, the developed method gave the enhancement factors of 46–51, the linear range of 0.40–200 μg L −1 , the detection limits (S/N = 3) of 0.12–0.24 μg L −1 , and the quantification limits (S/N = 10) of 0.40–0.85 μg L −1 . The recoveries for spiked NSAIDs (20 μg L −1 ) in water and urine samples were in the range of 77.3–104%. Besides, the MIL-53(Al) incorporated monolith was stable enough for 120 extraction cycles without significant loss of extraction efficiency. The developed method was successfully applied to the determination of NSAIDs in water and urine samples.
研究领域:
分析化学
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环境科学
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Luxiu Li,Ya Cui,Lingling Ren,Chunyang Li,Yehao Liu,Zhongxiu Jin
In this work, a simple and efficient dispersive solid-phase microextraction (DSPME) method based on magnetic dummy molecularly imprinted polymer nanoparticles (MMIPs) was developed for the extraction of five phthalate monoesters (mPAEs) from human urine samples. The MMIPs synthesized by sol–gel reaction and surface imprinting were analyzed by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometry, and thermogravimetric analysis. The obtained results demonstrate that the MMIPs prepared herein are characterized by high selectivity, good adsorption capacity (124.5 mg/g), excellent reusability (over five sorption–desorption cycles), and fast adsorption rates (about 30 min to equilibrium) with respect to five mPAEs. In addition, the MMIPs can be easily separated from the sample solutions due to their good magnetic susceptibility (34.0 emu/g). Using MMIPs as sorbent material in DSPME, five mPAEs in three human urine samples were extracted, and then analyzed by high-performance liquid chromatography (HPLC). Based on the obtained results, this method shows good linearity in the range of 1–100 mg/L for monomethyl phthalate, monoethyl phthalate, and monobutyl phthalate, in the range of 1–80 mg/L for monobenzyl phthalate, and in the range of 1–50 mg/L for monoethylhexyl phthalate. Moreover, good recovery (80.0%–101.0%) of all five mPAEs was observed at three spiked level with relative standard deviations of 0.6–6.6%. Overall, the results reported herein indicate that the prepared MMIPs can be used as sorbent material for the dispersive solid-phase microextraction of phthalate monoesters in human urine samples.
研究领域:
分析化学
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环境科学
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高影响因子
LUMINESCENCE
·
2022
·Vol. 37
·期 8
·1267-1274
·
IF 2.613
Qi Wang,Bin Zhu,Yejiao Han,Xin Yang,Yanan Xu,Ying Cheng,Taotao Liu,Jiana Wu,Shengling Li,Lifeng Ding,Jingjing Bai,Yulan Niu
In this work, carbon dots (CDs) was easily synthesized from aspartic acid through a pyrolysis method. Based on their favourable fluorescence properties, CDs were utilized to design a metal ion-mediated fluorescent probe for N -acetyl- l -cysteine (NAC) detection. The fluorescence intensity of CDs was firstly quenched by manganese ions (Mn 2+ ) through static quenching effect and subsequently restored by NAC via the combination with Mn 2+ due to the coordination effect. Therefore, the fluorescent turn-on sensing of NAC was actuated based on the fluorescence quenching stimulated by Mn 2+ and recovery induced by coordination. The fluorescence recovery efficiencies showed a proportional range to the concentration of NAC in the range 0.04–5 mmol L −1 and the detection limit was 0.03 mmol L −1 . Furthermore, this metal ion-mediated fluorescent nanoprobe was applied to human urine sample detection and the standard recovery rates were located in the range 97.62–102.34%. This was the first time that Mn 2+ was used to construct a fluorescent nanoprobe for NAC. Compared with other heavy metal ions, Mn 2+ with good biosecurity prevented the risk of application, which made the nanoprobe green and biopractical. The facile synthesis of CDs and novel metal ion-mediated sensing mode made it a promising method for pharmaceutical analysis.
研究领域:
分析化学
·
纳米技术
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生物化学
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PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 13
·5635-5640
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IF 1.821
Yajing Lei,Xiaoyan Yao,Dai Zhang,Lifang Zhou,Weixian Lu,Shuqing Chen
Salbutamol (SAL) is used to promote animal growth, because it increases feeding efficiency and carcass lean percentage. SAL has been banned in many countries because of the potential hazard to human health. To investigate the SAL residue level in humans, a sensitive direct competitive ELISA was developed. Under the optimal experimental conditions, the limit of detection (LOD) for the assay was 0.01 ng mL−1, and the standard curve was constructed at concentrations of 0.01–100 ng mL−1. We used the developed assay to detect the urinary concentration of SAL in 1648 participants. SAL was detected in 96% of participants with a concentration ranging from the LOD to 30.83 ng mL−1. Pregnant women were found to have a lower SAL concentration than non-pregnant women. Sources of exposure, risk assessment and measures for reducing the exposure level require further investigation.
研究领域:
分析化学
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毒理学
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公共卫生
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高影响因子
Green Processing and Synthesis
·
2024
·Vol. 13
·期 1
·
IF 3.8
Jianuo Sun,Haokun Ke,Jinghan Wang,Xianchao Du,Hongxia Hao,Hong Zhou
Based on the surface plasmon resonance imaging (SPRi) instrument, we established a new method of analyzing morphine in urine by processing a calibration curve. According to an inhibition immunoassay, gradient concentration of morphine and morphine-BSA fixed on the chip competitively combine with morphine antibody on the chip. Given the three mathematical models, the data of SPRi signals generated from SPRi with morphine was processed to obtain the calibration curve. Ultrafiltration was used to pretreat blank urine samples with adding morphine, and then investigated the advantages and disadvantages of each model. With a limit detection of 6.57 ng·mL −1 , the method and mathematical models can provide robust support for SPRi sensors used in further environmental detection, such as the epidemiological study of sewage.
研究领域:
分析化学
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生物化学
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环境科学
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传感器与执行器
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药理学
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高影响因子
危险品化学品经营许可证（带存储）