JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Yehong Han,Mingyu Wang,Chunliu Yang,Zihui Zhao,Yanan Yuan,Hongyuan Yan
Osimertinib (OSIM) is widely used as a mainstream drug for the treatment of non-small cell lung cancer (NSCLC). However, the lack of a rapid extraction and detection method for OSIM and its metabolite, AZ-5104, has limited clinical drug metabolism and drug resistance research because the drug is unstable. In this study, a new ionic liquid hybrid hierarchical porous material (IL-HHPM) was synthesized with hierarchical porous structures, including micropores (1.6–2.0 nm), mesopores (2.0–50.0 nm), macropores (50.0–148.7 nm), and multiple functional groups via a one-step hydrothermal method using silanized ionic liquids (IL) as functionalized hybrid monomer. The IL-HHPM has the advantages of a high specific surface area (437.4 ± 4.6 m 2 g −1 ), sizable pore volume (0.74 cm 3 g −1 ), and fast mass transfer, additionally, the IL-HHPM adsorbed OSIM and AZ-5104 via π-π interactions and hydrogen bonding. OSIM and AZ-5104 were rapidly extracted and measured in human urine using rapid and miniaturized centrifugal spin-column extraction (MCSCE), which was based on the IL-HHPM. The optimized factors for the extraction recoveries of OSIM and AZ-5104 were adsorbent dosage (8.0 mg), sample volume (0.5 mL), and operation time (9.0 min), and markedly reduced the adsorbent dosage and operation time. The IL-HHPM-MCSCE-HPLC method displayed good linearity (0.02−5.00 μg mL −1 , r ≥ 0.9997), satisfying accuracy (spiked recoveries of 87.7%−100.0%), and good precision (RSDs ≤ 7.0%). The developed method is rapid, sensitive, and reproducible for the simultaneous determination of trace level of OSIM and AZ-5104 in human urine.
研究领域:
分析化学
·
药理学
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Zhuangxin Wei,Pan Wang,Xiaohua Tian,Wei sun,Jianming Pan
“Tailor-made” formation of complementary binding sites via lock-and-key molecular binding mechanism is critical for selective adsorption of target molecules in environmental and chemical engineering fields. However, the innovative design of molecularly imprinted polymers (MIPs) remains a challenging task. Here, photo-crosslinked functional polymer building blocks consisting of monomeric methacrylic acid (MAA), 1-(vinylbenzyl)thymidine (VBT) and post-crosslinked monomeric cinnamoyloxyethyl methacrylate (CEMA) were firstly synthesized. MIPs beads (PC-MIPs) were then prepared by emulsion droplet microreactor, and finally their precise recognition of 2'-deoxyadenosine (dA) was investigated by batch mode adsorption. The prepared PC-MIPs microspheres have a large number of voids on the surface with interconnected cavities inside, and the bubble-like framework inside may be caused by the full encapsulation of bubbles from the low boiling point isolated phase. Due to the high density of accessible imprinted sites and strong interaction with dA, the maximum monolayer adsorption capacity of PC-MIPs was calculated to be 134.5 μmol g −1 for dA, surpassing most of the reported dA-imprinted sorbents. In addition, unique advantages of the post-crosslinking strategy combined with the emulsion template method exhibited good selectivity (imprinting factor higher than 2.71) and high stability, as well as excellent recyclability toward dA. Moreover, 82.4 % of dA was extracted by PC-MIPs from spiked human urine samples. Therefore, our work demonstrated an alternate strategy to design polymers bearing precise molecular recognition capability, and provided a new perspective for dA enrichment from biological samples.
研究领域:
环境工程
·
化学工程
·
生物分析
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Xiaobin Zhang,Suling Zhang,Ting Lü,Wenfei Huang,Hongting Zhao,Hui Wang,Dong Zhang,Weixuan Yao
Anabolic steroids and β-agonists are commonly prohibited substances found in doping control studies; therefore, the determination of anabolic substances in biological samples is crucial. To analyze the anabolic compounds in urine, an adsorbent, polyethylene glycol (PEG)-grafted magnetic nanoparticle material (Fe 3 O 4 @SiO 2 -PEG), with low toxicity and strong biocompatibility was prepared in this investigation. Compared to those of Fe 3 O 4 and Fe 3 O 4 @SiO 2 , the grafted PEG chains (approximately 5.4 wt.%) on the magnetic nanoparticles improved the extraction efficiencies by factors of 3.9–17.0 and 2.5–2.9, respectively, likely due to the electrostatic attraction and hydrogen bonding. To achieve maximum extraction efficiency, several extraction parameters were optimized, including the kind and volume of desorption solvent, pH, and the extraction and desorption time. The standard curves were linear within the range of 0.5–20 μg/L for methyltestosterone and trenbolone, and 0.02–5 μg/L for clenbuterol. The limits of detection for the three drugs were 0.01–0.12 μg/L. The limits of quantification were 0.02–0.40 μg/L. The levels of precision of the optimized method were assessed based on the respective intra- and inter-day and batch-to-batch relative standard deviations in the ranges of 3.2–5.2 % ( n = 5), 5.9–11.3 % ( n = 4), and 6.7–9.2 % ( n = 3). The Fe 3 O 4 @SiO 2 -PEG nanoparticles could exclude urine matrix interferences (matrix effect of 91.8–98.1 %) and achieve satisfactory recoveries (75.5–116.1 %), affording sensitive and accurate determination of trace anabolic substances in urine.
研究领域:
分析化学
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药物分析
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 12
·4421-4429
·
IF 1.938
Wenyue Zou,Pierre Dramou,Lien Ai Pham-Huy,Kai Zhang,Jia He,Chuong Pham-Huy,Deli Xiao,Hua He
Water-compatible magnetic molecularly imprinted polymers (M-MIPs) for extraction and pre-concentration of anthracyclines (ANTs) from urine have been successfully synthesized by a non-covalent method using epirubicin (EPI) as a dummy template, methacrylamide as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker. The obtained M-MIPs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). Adsorption kinetic and isotherm studies were carried out, which indicated that the M-MIPs displayed a rapid dynamic process and a high adsorption capacity. The adsorption behavior was discussed in detail, showing that it could be described as a chemisorption process and both external surface diffusion and intra-particle diffusion contributed to the adsorption mechanism. Furthermore, the binding sites were found to be heterogeneous for M-MIPs, while homogeneous for M-NIPs. The selectivity of M-MIPs demonstrated higher affinity for target EPI and EPI-analogues over other structurally unrelated compounds. A rapid solid-phase extraction (SPE) method using M-MIPs as sorbents coupled with high performance liquid chromatography (HPLC) was established for simultaneous determination of ANTs in urine samples. The recoveries ranged from 93.9% ± 5.2% to 100.0% ± 3.4% with the limit of detection of 0.6–2.4 ng mL−1. Moreover, the M-MIPs could be regenerated, which could be utilized for several cycles with no obvious decrease in the adsorption capacity. The results indicated that the proposed method is a practical approach for simultaneous determination of ANTs in urine.
研究领域:
分析化学
·
药学
·
医学检验
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）