JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2024
·Vol. 333
·期 3
·1357-1365
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IF 1.5
Mei Lingzi,Shao Hui,Cui Lingzhi,Yang Jinmin,Zhang Lei
A novel method for rapid radioanalytical measurement of uranium and plutonium in 100ml emergency urine samples was developed, through optimized selection of the separation and purification process and a combined measurement with ICP-TOF-MS and alpha spectrometry. The typical analytical time is within 6 h, the recovery rates are more than 80%, and the minimum detection activity concentrations are 3.4 × 10 −4 Bq/L for 238 U and 2.5 × 10 −3 Bq/L for 239 Pu respectively. The spiked test shows that this method can be used for quantitative evaluation of the activity concentration of 238 U and 239 Pu in urine samples accurately.
研究领域:
放射化学
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环境科学
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医学检测
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DOI
高影响因子
SENSORS
·
2020
·Vol. 20
·期 5
·1331
·
IF 3.275
Yue Wang,Huan Feng,Haibo Li,Xinyi Yang,Hongmin Jia,Wenjun Kang,Qingtao Meng,Zhiqiang Zhang,Run Zhang
Quick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (L-Cu2+) based on a benzocoumarin-Cu2+ensemble has been developed for the detection of biothiols (Cys, Hcy and GSH) in human urine. The chemosensing ensemble (L-Cu2+) contains a 2:1 stoichiometry structure between fluorescent ligandLand paramagnetic Cu2+.Lwas found to exclusively bond with Cu2+ions accompanied with a dramatic fluorescence quenching maximum at 443 nm and an increase of an absorbance band centered at 378 nm. Then, the in situ generated fluorescence sluggish ensemble,L-Cu2+, was successfully used as a chemosensor for the detection of biothiols with a fluorescence “OFF-ON” response modality. Upon the addition of biothiols, the decomplexation ofL-Cu2+led to the liberation of the fluorescent ligand,L, resulting in the recovery of fluorescence and absorbance spectra. Studies revealed thatL-Cu2+possesses simple synthesis, excellent stability, high sensitivity, reliability at a broad pH range and desired renewability (at least 5 times). The practical application ofL-Cu2+was then demonstrated by the detection of biothiols in human urine sample.Keywords:chemosensor;biothiols;displacement;detection;urine samples
研究领域:
分析化学
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生物医学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
·
IF 3.412
Liang-Liang Qian,Rui-Xian Li,Hai-Tao Gao,Chun-Min Wang,Lei Li,Qian Xu,Jian Li
In this work, male rats were exposed to multiple phthalate esters (MIXPs) in a long-term low-dose model for the early evaluation of reproductive toxicity. An ananlysis method with better sensitivity, accuracy and precision was established to determine the five sex hormones (androstenedione, testosterone, dehydroepiandrosterone, dihydrotestosterone, and estrone) in collected urine samples. The results showed that all the analytes in the MIXPs treated group changed in a time-dependent manner. Specifically, estrone significantly decreased from the 30th day and the other four changed from the 30th day and then significantly increased on the 60th day, while no obvious changes were found in the control group. Therefore, a possible way was provided for the early evaluation of male reproductive toxicity induced by Phthalate esters (PEs) . The reliability of judgment was improved by observing the changes of five target hormones simultaneously. Furthermore, good compliance was predicted for the practical application due to the noninvasive and convenient urine sample collection.
研究领域:
毒理学
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分析化学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Xuehong Min,Yuan Zhuang,Zhenyu Zhang,Yongmei Jia,Abdul Hakeem,Fuxin Zheng,Yong Cheng,Ben Zhong Tang,Xiaoding Lou,Fan Xia
We demonstrate an ultrasensitive microRNA detection method based on an extremely simple probe with only fluorogens but without quencher groups. It avoids complex and difficult steps to accurately design the relative distance between the fluorogens and quencher groups in the probes. Furthermore, the assay could accomplish various detection limits by tuning the reaction temperature due to the different activity of exonuclease III corresponding to the diverse temperature. Specifically, 1 pM miR-21 can be detected in 40 min at 37 °C, and 10 aM (about 300 molecules in 50 μL) miR-21 could be discriminated in 7 days at 4 °C. The great specificity of the assay guarantees that the real 21 urine samples from the bladder cancer patients are successfully detected by our method.
研究领域:
癌症研究
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 32
·3094-3102
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IF 3.532
Linan Guo,Yahui Wang,Meng Yan,Xinxin Li,Xinyao Jiang,Manman Wang,Qian Wang,Xuesheng Wang,Yulan Hao
A cerium (Ce)-doped metal–organic framework composite (Ce/DUT-52) was prepared by using a solvothermal method and was explored as a sorbent for dispersive solid phase extraction (DSPE) of three estrogens (α-estradiol, estrone, and hexestrol) in human urine samples. After doping with Ce(III), Ce/DUT-52 exhibited more attractive features involving a higher specific surface area (774.7 m2 g−1) and zeta potential (31.4 mV), which made it an efficient adsorbent for the separation and enrichment of estrogens. The factors influencing DSPE efficiency such as the adsorbent amount, extraction time, pH, NaCl concentration, elution solvent and elution volume were investigated in detail. Under the evaluated conditions, Ce/DUT-52 showed good reusability (n = 6, RSDs ≤ 4.8%). Notably, the cofunction of electrostatic interaction, hydrophobic interaction, hydrogen bonding and π–π interaction might play major roles between estrogens and Ce/DUT-52. Finally, coupled with high-performance liquid chromatography (HPLC), a fast and sensitive method was established, which provided low limits of detection (1.5–2.0 ng mL−1), wide linear ranges (3–500 ng mL−1) and satisfactory recoveries (79.8–96.1%). The results demonstrated that Ce/DUT-52 had excellent adsorption ability to the targets and the developed method provided an alternative strategy for the determination of trace estrogens or other compounds with similar chemical structures in urine samples.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 11
·期 3
·353-358
·
IF 2.073
Lizhen Liu,Zhi Mi,Qin Hu,Caiqing Li,Xiaohua Li,Feng Feng
In this article, a green, simple and economic method based on microwave treatment of glycine and urea was developed to synthesize water-soluble carbon dots (CDs) with strong fluorescence. The obtained CDs were characterized by transmission electron microscopy (TEM), UV-vis absorption spectroscopy, fluorescence spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The results indicate that the as-synthesized CDs have an average diameter of 3.2 nm and exhibit excitation-dependent emission behavior. The surface of the CDs is rich in amine, amide, hydroxyl and carboxyl groups, which endow the CDs with strong fluorescence and good water solubility. We found that the as-synthesized CDs can be used as an effective fluorescence probe for morin detection based on the fluorescence quenching of CDs. The linear range for morin detection is 0.4–60 μM with a limit of detection (LOD) of 0.12 μM. The CD-based fluorescence sensor shows good selectivity and sensitivity toward morin sensing. Finally, the proposed sensing method was successfully applied to detect morin in human urine samples with recoveries of 97.7–104%.
研究领域:
分析化学
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材料科学
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生物医学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2020
·
IF 3.594
Kai Hu,Tiantian Pang,Yanmei Shi,Jiamin Cheng,Yanjie Huang
In this work, a facile strategy for the preparation of magnetic porous organic framework (Ni@POFs) was proposed by the reduction of Ni ions on its skeleton in situ. The properties of the material were confirmed by X-ray diffraction, vibrating sample magnetometer, infrared spectroscopy, transmission electron microscopy and scanning electron microscopy. The prepared Ni@POFs exhibits a strong magnetism and high extraction efficiency for targeted alkaloids. All the key factors that affect the extraction/desorption efficiency of the alkaloids onto the Ni@POFs material, such as sorbent amount, extraction time, sample pH and desorption condition, were investigated with the help of response surface methodology. In combination with magnetic solid phase extraction and ultra performance liquid chromatography-tandem mass spectrometry analysis, a novel method for the quantitative analysis of alkaloids in urine samples was proposed. The limits of detection and limits of quantitation for the eight alkaloids were found to be in the range of 0.9–6.0 and 3.0–20 pg mL −1 , respectively. The recoveries of spiked urine samples were in the range of 93.5–99.2% with RSDs less than 6.9%. The proposed method was successfully applied to analyze the metabolites in patient’s urine after the oral administration of berberine.
研究领域:
分析化学
·
药理学
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DOI
高影响因子
Methods and Applications in Fluorescence
·
2019
·Vol. 8
·期 1
·015005
·
IF 2.94
Shu Juan Zhuo,Jing Fang,Jing Wang,Chang Qing Zhu
In this paper, a simple and convenient fluorescence method for detection of uric acid (UA) based on Ag–doped carbon quantum dots (Ag–CQDs) is developed. The Ag–CQDs contain Ag species could bond with UA, which promoted the electron and/or energy transfer and produced high quenching extent. Thus, the fluorescence intensity of Ag–CQDs decreased in the presence of UA. Under the optimal condition, the UA sensor exhibited a linear response in the range of 0.005–100 μM with a detection limit of 0.35 nM (S/N = 3). Ag–CQDs demonstrated high sensitivity and rapid response to UA over a wide range of concentration indicating their great potential as a fluorescent probe for chemical sensing. Moreover, this method has successfully applied to detect UA in human urine samples.
研究领域:
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2013
·
IF 2.487
Lin Song,Wanchen Li,Guoxia Li,Dianjun Wei,Peng Ge,Guizhen Li,Fang Zheng,Xuguo Sun
This study explored a method that can rapidly detect bacteria in urine samples for the auxiliary determination of urinary tract infections (UTIs). Urine samples from patients with UTIs (230 cases) were obtained using aseptic technique. The urine biochemical assay was then carried out using an automated urine analyzer for all the urine samples. Bacterial species were identified by a combination of bacterial culture technique , morphological observation and the BACT-IST microbial identification/susceptibility analysis system. The most common seven species of bacteria in the study included Escherichia coli , Klebsiella pneumoniae , Pseudomonas aeruginosa , Enterobacter cloacae , Enterococcus faecalis , Staphylococcus aureus and Staphylococcus epidermidis . Bacterial samples were suspended in sample buffer solutions and separated by the “three-plug-injection” method using capillary electrophoresis (CE). Each species of bacteria appeared as a bacterial peak. The mixture of the seven species also provided only one peak. Further analysis showed that the concentration limit for the “three-plug-injection” method is 10 6 colony forming units (CFU)/mL, and there is a good linear relationship between the peak height and bacterial concentration ( R 2 = 0.99). The effect of urine composition on CE results was also investigated. The results showed that urine composition, i.e., proteins, white blood cells (WBCs) and red blood cells (RBCs), affected the peak retention time but could not affect the separation of bacteria. The results demonstrated that the bacteria in urine samples can be detected within 10 min by the “three-plug-injection” method using CE. The “three-plug-injection” method is therefore suitable for the rapid detection of organisms in clinical urine samples from UTIs.
研究领域:
医学检验
·
微生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
·
2023
·
IF 1.8
Qin Yang,Shenshu Chen,Yaqing Li,Taifeng Jiao,Lianghong Cheng,Quan Yu,Xinqiong Lu
In this study, we introduce the design, characterization, and application of an integrated thermal desorption corona discharge ionization (TD-CDI) device, which was developed for direct mass spectrometry (MS) analysis of drugs in solid or liquid samples. The structure and operational parameters of the TD module were optimized through simulation and experimental investigations. For the CDI module, a hollow needle was used as the discharge electrode and sample inlet , allowing self-aspiration and efficient ionization of the desorbed samples. The TD-CDI device was tested and characterized using different mass spectrometers , exhibiting satisfactory stability and quantitative performance. Owing to its compact structure, it can be coupled to a miniature MS instrument to implement rapid identification of various drugs in urine solutions.
研究领域:
分析化学
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医学检测
·
仪器科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xin-Ran Guo,Yan-Xin Guo,Juan Chen,Xin-Yue Chen
In this work, a simple and sensitive ratiometric fluorescence probe to detect isoniazid (INH) was developed on the basis of carbon dots and MnO 2 nanosheets (MnO 2 NS). Nitrogen-doped carbon dots (FMT-CDs) were synthesized by microwave method using formononetin (FMT) in ammonia water. The oxidase-like activity of MnO 2 NS was utilized to oxidize non-fluorescent o -phenylenediamine (OPD) to 2, 3-diaminophenazine (DAP) with orange fluorescence. It was demonstrated that DAP could effectively quench the fluorescence of FMT-CDs through the inner filtering effect (IFE). Due to the reducing nature of INH, MnO 2 NS was decomposed and thus lost its oxidase-like activity after the addition of INH. Therefore, the production of DAP was reduced, and the fluorescence intensity was decreased, while IFE on FMT-CDs was weakened and the fluorescence of FMT-CDs was recovered. Based on the above principle, a sensitive method for the detection of INH was established based on the fluorescence intensity ratio of the two fluorescent substances, FMT-CDs and DAP, and was successfully applied to the detection of INH in human urine samples. The limit of detection (LOD) could reach to 0.16 μM in human urine samples.
研究领域:
分析化学
·
医学诊断
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Shuqi Zhou,Ying Gan,Liubing Kong,Jiadi Sun,Tao Liang,Xinyi Wang,Hao Wan,Ping Wang
Adenosine has received great attentions acting as a potential biomarker for monitoring lung cancer. Most of the reported studies for adenosine detection require large instruments and complicated procedures. Herein, a sensitive, rapid and in-situ colorimetric aptasensor was developed for adenosine detection. Moreover, a homemade biomimetic electronic-eye (E-eye) was established and utilized as a portable in-time detection equipment. The entire measurement can be completed within 20 min, including the combination of aptamer with adenosine or AuNPs and the detection of adenosine. Four different kinds of aptamer were compared and the results showed that the AuNPs-aptamer-biotin system was the most stable and with the widest detection range of 5.0 μM–60.0 μM and the lowest LOD of 0.17 μM. Moreover, the artificial urine samples were also tested with a linear range from 5.0 to 50.0 μM and a LOD of 0.48 μM. The results validated that the aptasensor together with the E-eye can be a promising platform for adenosine detection.
研究领域:
生物化学
·
医学诊断
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）