Rice Science
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2023
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IF 4.8
Yang Xiaozheng,Liu Yusong,Huang Jing,Tao Ye,Wang Yifeng,Shen Renfang,Zhu Xiaofang
Phosphorus (P) starvation in rice facilitates the reutilization of root cell wall P by enhancing the pectin content. NaCl modulates pectin content, however, it is still unknown whether NaCl is also involved in the process of pectin regulated cell wall P remobilization in rice under P starved conditions. In this study, we found that 10 mmol/L NaCl increased the shoot and root biomasses under P deficiency to a remarkable extent, in company with the elevated shoot and root soluble P contents in rice. Further analysis indicated that exogenous NaCl enhanced the root cell wall P mobilization by increasing the pectin methylesterase activity and uronic acid content in pectin suggesting the involvement of NaCl in the process of cell wall P reutilization in P starved rice roots. Additionally, exogenous NaCl up-regulated the expression of P transporter OsPT6, which was induced by P deficiency, suggesting that NaCl also facilitated the P translocation prominently from root to shoot in P starved rice. Moreover, exogenous abscisic acid (ABA) can reverse the NaCl-mediated mitigation under P deficiency, indicating the involvement of ABA in the NaCl regulated root cell wall P reutilization. Taken together, our results demonstrated that NaCl can activate the reutilization of root cell wall P in P starved rice, which is dependent on the ABA accumulation pathway.
研究领域:
植物生理学
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分子生物学
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农学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Brenda Bezus,Juan Carlos Contreras Esquivel,Sebastián Cavalitto,Ivana Cavello
An Antarctic yeast was cultivated to produce and enzymatic extract with polygalacturonase activity, whose biochemical properties were studied. It assisted pectin extraction from lime pomace at 20 °C. The extract produced by Tausonia pullulans 8E had an optimum temperature of 40 °C and optimum pH of 5.0. At 20 °C, it displayed 54% of relative activity. A good pH-stability and thermostability were observed. Hg +2 and Co +2 inhibited its activity in 40 and 11%, respectively. Pectin was obtained from lime pomace at 20 °C, with 15% of yield after 120 min extraction. Uronic acid (46%) and neutral sugars (53%) were determined. Pectin's molecular weight was estimated in the order of 100,000 g mol −1 . By anion-exchange chromatography, pectin-linked and free neutral sugars were observed. The high-methoxyl pectin was used to prepare low-calorie gels. It was demonstrated that this cold-active enzyme allows enzymatic-assisted pectin extraction at 20 °C.
研究领域:
食品科学
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生物技术
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生物化学
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酶学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2015
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IF 6.409
Yunlei Zhou,Huanshun Yin,Jie Li,Bingchen Li,Xue Li,Shiyun Ai,Xiansheng Zhang
MicroRNAs play crucial role in post-transcriptional regulation for gene expression in animals, plants, and viruses. For the better understanding of microRNA and its functions, it is very important to develop effectively analytical method for microRNA detection. Herein, a novel electrochemical biosensor was fabricated for sensitive and selective detection of microRNA based on poly(U) polymerase mediated isothermal signal amplification , where poly(U) polymerase can catalyze the template independent addition of UMP from UTP to the 3’ end of RNA. Using this activity, the target microRNA can be successfully labeled with biotin conjugated UMPs at its 3′-end using biotin conjugated UTP (biotin-UTP) as donor. Then, the avidin conjugated alkaline phosphatase can be further captured to the 3′-end of the target microRNA based on the specific interaction between biotin and avidin. Finally, under the catalytic activity of alkaline phosphatase, the substrate of p -nitrophenyl phosphate disodium salt hexahydrate can be hydrolyzed to produce 4-nitrophenol. According to the relationship between the electrochemical signal of p -nitrophenol and the concentration of microRNA-319a, the content of microRNA-319a can be detected. This signal amplification method is simple and sensitive. The developed method can detect as low as 1.7 fM microRNA and produce precise and accurate linear dynamic range from 10 to 1000 fM. The fabricated biosensor was further applied to detect the expression level change of microRNA-319a in rice seedlings after incubation with five kinds of different phytohormones.
研究领域:
生物化学
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分析化学
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分子生物学
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植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2024
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IF 11
Xiaofei Liang,Mengqiu Liu,Anni Yao,Wanchen Cui,Yan Wei,Sheng Guo,Jialun Duan,Hongjie Kang,Xueyi Zhou,Shulan Su,Heiying Jin,Fang Zhang,Jin-ao Duan
In this study, the microbial fermentation characteristics and effects of neutral and acidic Lycii fructus polysaccharides (LFPs) on gut microbial composition were investigated using pure cultures of selected probiotic strains and mixed cultures of human fecal microflora in vitro . The results revealed that LFPs could significantly promote the growth of probiotics, with a faster proliferation rate in the early stages of fermentation. When LFPs were degraded and utilized by the gut microbiota, neutral arabinogalactans were more easily fermented, while acidic pectin polysaccharides exhibited a higher degree of anti-fermentation. LFPs were selectively modulated by microbiota; acidic polysaccharides promoted the growth of potentially beneficial bacteria, such as Lachnospira and Bacteroides vulgatus , while neutral polysaccharides significantly improved the relative abundances of Bifidobacterium longum and Bifidobacterium pseudocatenulatum . In summary, the results revealed that different types of LFPs regulate the gut microbiota in a structure-dependent manner, with uronic acid play a key role in selective regulation. This study aimed to better understand the fermentation characteristics and saccharide preferences of gut microbes for LFP structures and provide a theoretical basis for the relationship between the chemical structural and probiotic effects of LFPs.
研究领域:
微生物学
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营养学
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食品科学
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胃肠病学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Fei Qu,Zhuo Wang,Cong Li,Dafeng Jiang,Xian-en Zhao
Herein, a novel peptidyl probe is first synthesized by the amide reaction between glutathione-capped gold nanoclusters (GSH-AuNCs) and positive charged arginine-rich peptide (Arg 9 ), and it is called Arg 9 -GSH-AuNCs. Polyuridylic acid (polyU), as a polyanion , is firstly used to induce the aggregation-induced emission enhancement (AEE) of positive charged Arg 9 -GSH-AuNCs through electrostatic attraction . The peptide bond on the carboxyl terminal of the arginine residues can be recognized and cleaved by trypsin. Hence, polyU is hard to induce the aggregation of peptidyl probe again, and the AEE from GSH-AuNCs becomes weak. It is an ideal platform to detect trypsin and screen trypsin inhibitors . The fluorescent peptidyl probe exhibits excellent sensitivity for trypsin. A good linear range of trypsin is obtained from 1 to 50 ng·mL −1 with the limit of detection (LOD) 0.31 ng·mL −1 , which is lower than those of most reported works. Furthermore, the inhibitory effects of the extracts from foods (i.e., soybean seed flour, black soybean seed flour, mung bean seed flour, and azuki bean seed flour) on the trypsin activity have been studied. The half maximal inhibitory concentration (IC 50 ) values of extracts from soybean seed flour, black soybean seed flour, mung bean seed flour, and azuki bean seed flour are 4.5, 9.3, 11.6, 20.9 μg·mL −1 , respectively. The screening results show that these extracts from foods all have inhibitory effects on trypsin. Among them, the extract from soybean seed flour exhibits significant inhibitory effect.
研究领域:
分析化学
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生物化学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
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