Journal of Building Engineering
·
2024
·
IF 6.7
Zhitang Li,Kaiyue Hu,Ganlin Feng,Wujian Long,Qiling Luo,Cang Chen
Concrete repair in marine environments is influenced by various factors, particularly underwater pressure and dynamic water flow. Traditional materials used for underwater concrete repair suffer from issues such as dilution, dispersion, susceptibility to erosion by moving water , and poor grout retention. In this study, a novel biopolymer-based undersea crack repair material composed of quaternized chitosan (Hydroxypropyl trimethyl ammonium chloride chitosan, HACC), sodium alginate (SA), urease, and aggregates was developed. It investigated the impact of biopolymers on urease's physiological and biochemical properties, as well as its mineralization capacity. Additionally, It conducted a comprehensive evaluation of the fluidity, rheological behavior , grout retention, mechanical properties, and anti-permeability rate of the bio-grouting material. The results showed that the optimized bio-grouting material exhibited suitable viscosity and fluidity. After injection, it rapidly formed a cohesive seal, with a grout retention rate exceeding 90 % at 48 days. The water resistance of the repaired concrete reached 93 %. Microstructural analysis using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) revealed strong electrostatic interactions between HACC and SA molecular chains . These interactions encapsulated mineralization products and sand particles, resulting in a denser microstructure.
研究领域:
土木工程
·
生物化学
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产品关联
DOI
高影响因子
ANALYST
·
2017
·Vol. 142
·期 20
·3986-3992
·
IF 3.885
Hao-Hua Deng,Xiao-Qing Zheng,Yan-Yu Wu,Xiao-Qiong Shi,Xiu-Ling Lin,Xing-Hua Xia,Hua-Ping Peng,Wei Chen,Guo-Lin Hong
We herein report the intrinsic alkaline peroxidase-like activity exhibited by CuO nanoparticles when 3-(4-hydroxyphenyl)propionic acid was employed as a substrate. Based on this observation, a fluorometric assay method with a low detection limit of 0.81 μM was established for H2O2 determination under alkaline conditions. Notably, ammonia was found to inhibit the alkaline peroxidase-like activity of the CuO nanoparticles. Thus, a sensing platform for the determination of urea and urease was successfully constructed, with the limits of detection for urea and urease being 27 μM and 2.6 U L−1, respectively. This platform was then applied for the detection of urea in human urine and urease in soil, which yielded satisfactory results. These results suggest that it is possible to extend the catalytic potential of peroxidase and its mimetics from acidic and neutral conditions to include activity in alkaline media as well. Furthermore, this strategy is a novel method for the analysis of urea and urease. The assay developed in this work is facile, inexpensive, convenient, and highly selective and sensitive. Therefore, it is expected that this system can serve as a template for the development of similar enzyme nano-mimics.
研究领域:
生物化学
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纳米生物学
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分析化学
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DOI
PubMed
高影响因子
Minerals
·
2018
·Vol. 8
·期 7
·296
·
IF 1.835
Jing Ouyang,Dawei Mu,Yi Zhang,Huaming Yang,Steven L. Suib
Barium carbonate (BaCO3) materials with the controllable morphology of nanoparticles were selectively loaded into the lumen halloysite nanotubes (abbreviated as Hal) by a urease assisted catalytic implementation strategy. The Hal mineral was pre-treated through leaching by hydrochloric acid (abbreviated as A-Hal), resulting in increased defect sites and zeta potential. The negatively charged urease was loaded inside the positively charged A-Hal lumen, and then through the decomposition of urea catalyzed by urease to produce carbonate ions and ammonia. When Ba2+diffused in, BaCO3particles were selectively synthesized in the lumen of A-Hal, the pore channels of A-Hal effectively controlled the growth and aggregation of BaCO3nanocrystals and their geometrical morphology. The obtained BaCO3/A-Hal-T was characterized by transmission electron microscopy, Fourier transformation infrared spectroscopy and X-ray diffraction, differential scanning calorimetry-thermogravimetry (DSC-TG). The BaCO3/A-Hal-T may provide a candidate for potential applications.Keywords:halloysite;urease;barium carbonate;selective decoration;nanoreactors
研究领域:
材料科学
·
生物催化
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产品关联
DOI
高影响因子
Environmental Technology & Innovation
·
2025
·
IF 7.1
Hua Huang,Xiaoyan Liu,Xianying Shan,Jingrong Yang,Lijuan Ding,Rongyu Zhang,Jiale Dai,Yanzhong Zhen,Jian Wang,Di Guo,Yu Liu,Wenfeng Jiang,Zhirui Niu
Since hydrothermal aqueous phase (HAP) contains abundant nutrients and humic-like substances, it is developed as nitrogen (N) fertilizer to improve plant growth and soil quality. However, significant NH 3 volatilization is a major drawback in dryland soil. To solve this problem, a compound fertilizer of HAP and urea was modified with various dosage (0.25 % - 5.0 %) of urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). The NH 3 , N loss, and lettuce growth were evaluated in parallel soil column and pot experiments. Results showed that increasing NBPT dosage increased soil dissolved organic N (DON) and ON while decreased NH 4 -N, NH 3 , N loss, urease activity, and ureC . NBPT successfully inhibited ammonification. Increasing NBPT dosage increased NO 3 -N but decreased PAO and AOA . The utilization rate of metabolic substrate (NH 4 -N) was more important for forming NO 3 -N, as evidenced by the negative correlation of NO 3 -N and NH 3 . The DON from HAP included N-heterocyclic compounds, which are toxic for nitrifiers. The toxic DON also significantly decreased root dry weight by 22.31 % when 5.0 % NBPT was added. The optimal additive dosages were 0.5 % and 2.0 % in the column and pot experiments, respectively, which decreased NH 3 and N loss by 45.38 % and 32.08 %. Further increasing NBPT dosage no longer decreased N loss but did harm to plant root. Therefore, this study demonstrated that an appropriate addition of NBPT to HAP fertilizer can effectively reduce ammonia volatilization and N loss. The innovative technique would promote the utilization of HAP resource within the context of sustainable dryland agriculture.
研究领域:
农业科学
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植物生理学
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产品关联
DOI
高影响因子
Microfluidics and Nanofluidics
·
2014
·Vol. 17
·期 4
·721-728
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IF 2.665
Tang Xiuwen,Liu Sufang,Wang Sifeng,Zhang Qin,Cheng Zhiyi
We have developed a sensitive enzyme inhibition assay on microfluidic system. The analysis was carried out by immobilizing enzyme through amide-bond or disulfide-bond formation with surface. Followed by detection of reaction product through fluorescent density were evaluated reusability, stability and sensitivity of microfluidic enzyme assay. The Michaelis–Menten parameters for free urease ( K
M = 1.027 μM) and for immobilized urease ( K
M = 1.528 μM of disulfide-bond immobilization; K
M ′ = 1.617 μM of amide-bond immobilization) showed reasonable activities maintained after immobilization with relative standard deviation (RSD) of 4.86 and 6.06 %, respectively. When compared enzyme activities of five repeated immobilization cycles through reversible disulfide-bond immobilization, we found that removal process and reversible immobilization did not affect efficiency of microreactor with RSD of 4.78 %. The IC 50 value 368 μM of inhibitor acetohydroxamic acid determined on chip showed good agreement with reported data 375 μM; K
i of 1.39 μM matched well with K
i of 1.46 μM via the traditional 96-microplate. This microfluidic could be extended to screening of enzyme inhibitor and enzymatic reaction kinetics study, which may be useful for clinical diagnostics, biotechnological research, drug discovery and other bioassays.
研究领域:
生物化学
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药物开发
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临床诊断
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Zhong-Fei Xue,Wen-Chieh Cheng,Lin Wang,Wenle Hu
Heavy metal contamination has caused serious threats to surrounding fragile environments and human health. While the novel microbial-induced carbonate precipitation (MICP) technology in the recent years has been proven effective in improving material mechanical and durability properties, the mechanisms remedying heavy metal contamination still remain unclear. In this study, the potential of applying the MICP technology to the lead remediation under the effects of urease activity and calcium source was explored. The values of OD 600 corresponding to the ureolytic bacterial activity , electrical conductivity (EC), urease activity (UA) and pH were applied to monitor the degree of urea hydrolysis. Further, the carbonate precipitations that possess different speciations and cannot be distinguished through test tube experiments were reproduced using the Visual MINTEQ software package towards verifying the validity of the proposed simulations, and revealing the mechanisms affecting the lead remediation efficiency. The findings summarised in this work give deep insights into lead-contaminated site remediation engineering.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2024
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IF 4
Hu Wang,Pengxiao Guo,Yadong Zhou,Chaochuang Yin,Yizhu Lei,Renshu Wang,Yinyan Wang,Tingting Wu,Ziao Zong
Two mononuclear Copper(II) complexes, identified as [Cu(C 10 H 8 NO 4 F)(C 6 H 7 N)] ( C1 ) and [Cu(C 10 H 8 NO 4 F)(C 12 H 8 N 2 )]·H 2 O·CH 3 OH ( C2 ), incorporating a tridentate Schiff-base ligand with an ONO coordination (C 10 H 8 NO 4 F = 5-fluoro-2-hydroxybenzylidene-L‑serine) and two unique auxiliary ligands (C 6 H 7 N = 4-methylpyridine and C 12 H 8 N 2 = 1,10-phenanthroline) were developed and detailed structurally. Density functional theory (DFT) evaluations of these complexes employed Becke's three-parameter hybrid (B3LYP) approach within the Gaussian 16 computational package. The outcomes from computational analysis corresponded with experimental data. Additionally, the inhibition capabilities of complexes C1 and C2 were evaluated in vitro targeting jack bean urease. Simultaneously, molecular docking was utilized to determine potential binding interactions. Experimental results and docking analyses showed that C1 had considerable inhibitory strength (IC 50 = 2.44 ± 0.15 μM) relative to the benchmark control, acetohydroxamic acid (IC 50 = 27.73 ± 2.93 μM). Furthermore, a 100-ns molecular dynamics assessment was performed to examine the interaction stability of urease with C1 and C2 , using the Desmond 2021 tool from Schrödinger. The correlation between structural configurations and inhibition efficacy was further explored through molecular docking, density functional theory analysis, and molecular dynamics evaluations.
研究领域:
生物化学
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计算化学
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药物化学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
·
IF 4.9
Chunxiao Zhang,Hongxiao Yu,Yuanzhi Zhang,Kun Wang,Mengying Chen,Xu Wang
Inspired by the natural world's fascinating capacity for color change, researchers have developed various responsive hydrogels that shift colors. Among these, hydrogels that react to pH changes are particularly notable for their unique properties. The urea-urease autocatalytic reaction, a key pH clock reaction, is especially effective in temporarily adjusting these hydrogels' properties and enabling color changes in response to stimuli. In this study, we synergize the urea-urease clock reaction with the pH-sensitive color transformation of neutral red (NR), thereby establishing an NR-urea-urease clock reaction system. This system exhibits a dynamic color cycle from yellow to red and back to yellow. By incorporating this color transition mechanism into polymer hydrogels, we successfully initiate a pH change within the system. This innovation paves the way for a smart material capable of displaying temporary information through enzymatic reactions . The temporal variation in information display is finely tuned by adjusting the urea and urease concentrations. Such reversible color-changing hydrogels hold promising prospects for applications in secure information transmission and dynamic camouflage, marking a significant step forward in systems chemistry and materials science.
研究领域:
材料科学
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系统化学
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生物化学
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产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2018
·
IF 3.057
Li Fu,Yong Huang,Wen Cai,Huaiwei Zhang,Jiehui Yang,Dihua Wu,Weitao Su
Improving the hydrophilicity of titania nanotubular arrays (TNAs) could promote their interfacial reaction rate when they interact under aqueous conditions. In this work, we proposed a simple hydrothermal method for Si and Sr doping. A significant hydrophilicity improvement could be observed in TNAs after the doping process. Therefore, Si@Sr/TNAs exhibited a superior urease loading and showed excellent chronocoulometric performance towards urea interaction.
研究领域:
材料科学
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电化学
·
纳米技术
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Zhicheng Liu,Shupeng Liu,Xiaoyu Zhao,Chenglong Xue,Yu Liu,Qi Shuai
Nanomotors, as a new type of micro-device, show good performance in terms of rapid transportation and deep penetration through their autonomous motion. However, their ability to efficiently break physiological barriers still remains a great challenge. Herein, we first developed a thermal-accelerated urease driven human serum albumin (HSA) nanomotor based on photothermal intervention (PTI) to achieve chemotherapy drugfree-phototherapy. The [email protected] ( [email protected] @ [email protected] ) is composed of a main body of biocompatible HSA, modified by gold nanorods (AuNR) and loaded with functional molecules of folic acid (FA) and indocyanine green (ICG). It promotes its own motion by breaking down urea to produce carbon dioxide and ammonia. In particular, the nanomotor is conveniently operated via near-infrared combined photothermal therapy (PTT)/ photodynamic therapy (PDT) to achieve an accelerated De value from 0.73 μm 2 s −1 to 1.01μm 2 s −1 , and ideal tumor ablation at the same time. In contrast to customary urease-driven nanodrug-stacked engine, this [email protected] has both targeting and imaging-guided capabilities, and finally achieves superior anti-tumor effects without chemotherapy drugs, through a “two-in-one” (motor mobility plus unique phototherapy in chemotherapy-drugfree phototherapy) strategy. This PTI effect with urease-driven nanomotors may offer further possibilities for future clinical applications of nanomedicines by enabling deep penetration and a subsequent chemotherapy-drugfree combination therapy strategy.
研究领域:
纳米技术
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生物医学工程
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癌症治疗
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药学科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Wanying Cui,Chunmiao Xia,Sheng Xu,Xinke Ye,Yihao Wu,Shukai Cheng,Rongli Zhang,Cuige Zhang,Zongcheng Miao
Dextran (Dex) and poly(ethylene glycol) (PEG)-based aqueous emulsions were stabilized using the self-assembled chitosan colloidal particles (CS CPs). Besides, the effects of pH, CS CPs concentration, polymer concentration, volume ratio of PEG solution to Dex solution, temperature, homogenizing speed and homogenizing time on the property of the W/W emulsions were investigated, respectively. In order to enhance the stability of the PEG-Dex emulsion, sodium tripolyphosphate was used to cross-link the CS CPs at the interface of emulsion droplets, which resulted in the stability duration for >1 year. Finally, the CS CPs were used as a support to immobilize urease and bovine serum albumin and a stabilizer to prepare W/W emulsion, which were then adopted as a catalysis system and as a spinning solution to fabricate drug-loaded nanofiber . This strategy potentially provides a new opportunity to encapsulate the active molecules at the water-water interface, and enrich the types of usable active molecules in the encapsulation in the W/W emulsions.
研究领域:
材料科学
·
化学工程
·
生物技术
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Zhiming Hou,Xinyu Ren,Zhuangzhuang Sun,Ruoqi An,Mingzhi Huang,Cen Gao,Mengying Yin,Guangxiu Liu,Dengqi He,Hongliang Du,Rongbing Tang
Bacteria-derived hydrogen sulfide (H 2 S) often contributes to the emergence of antibiotic-recalcitrant bacteria, especially persister (a sub-population of dormant bacteria), thus causing the treatment failure of Catheter-associated urinary tract infection (CAUTI). Here, an H 2 S harvester nanosystem to prevent the generation of persister bacteria and disrupt the dense biofilm matrix by the self-adaptive ability of shape-morphing is prepared. The nanosystem possesses a core-shell structure that is composed of liquid metal nanoparticle (LM NP), AgNPs, and immobilized urease. The nanosystem decomposes urea contained in urine to generate ammonia for eliminating bacteria-derived H 2 S. Depending on the oxidative layer of liquid metal, the nanosystem also constitutes a long-lasting reservoir for temporarily storing bacteria-derived H 2 S, when urease transiently overloads or in the absence of urine in a catheter. Depriving H 2 S can prevent the emergence of persistent bacteria, enhancing the bacteria-killing efficiency of Ga 3+ and Ag + ions. Even when the biofilm has formed, the urine flow provides heat to trigger shape morphing of the LM NP, tearing the biofilm matrix. Collectively, this strategy can turn trash (urea) into treasure (H 2 S scavengers and biofilm rippers), and provides a new direction for the antibacterial materials application in the medical field.
研究领域:
纳米技术
·
生物医学工程
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材料科学
·
微生物学
·
传染病学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）