iScience
·
2024
·
IF 4.6
Kerong Liu,Ying Gu,Xingnan Pan,Sha Chen,Jie Cheng,Le Zhang,Minkai Cao
Gestational diabetes mellitus (GDM) is a distinct form of diabetes that poses a significant threat to the health of both pregnant women and fetuses. The objective of this study was to investigate the impact of behenic acid (BA) on glucose metabolism, inflammation, and insulin resistance in GDM mice, and to elucidate the underlying molecular mechanism. Here, we demonstrated that daily administration of 10 mg/mL BA during pregnancy effectively ameliorated abnormal glucose metabolism in GDM mice and their offspring and improved birth outcomes in the offspring. Moreover, BA promoted the proliferation of islet β cells, restored their normal function, and augmented glucose uptake by skeletal muscle cells. Mechanistically, BA mitigated inflammation and insulin resistance in GDM mice by inhibiting activation of the TLR4/NF-κB signaling pathway. Our study provides compelling evidence supporting the efficacy of BA in improving GDM, suggesting its potential use as a dietary supplement for preventing and treating GDM.
研究领域:
内分泌学
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药理学
·
分子生物学
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DOI
PubMed
高影响因子
LANGMUIR
·
2026
·
IF 4.4
Yu Zang,Xiaomei Pei,Zhao Chen,Zhenggang Cui,Binglei Song
The unique molecular structure of the surfactant is expected to produce solutions with distinctive rheological properties. A novel cationic surfactant, N1,N1,N1,N3,N3-pentamethyl-N3-(3-(3-docosanamidopropanamido)propyl)propane-1,3-diammonium dibromide (C22-β-Ala-DQA), was synthesized. C22-β-Ala-DQA differs from conventional surfactants in that it contains a long saturated hydrophobic tail, two amide groups, and two quaternary ammonium head groups. It exhibits a low Krafft temperature (< 1 °C) and high aqueous solubility at 25 °C. When combined with sodium palmitate (SP), it forms transparent, viscoelastic solutions exhibiting pronounced elasticity at low concentrations across a broad range of molar ratios. Cryo-transmission electron microscopy (Cryo-TEM) imaging confirms the formation of ultralong wormlike micelles with a uniform cross-sectional diameter of ∼7 nm. Notably, the C22-β-Ala-DQA:SP system at a 1:1.5 molar ratio demonstrates significant thermo-thickening behavior between 30 and 80 °C. The zero-shear viscosity (η0) of a 75 mM solution reaches a maximum of 632.5 Pa·s at 80 °C, which is unusually high for viscoelastic surfactant systems. This behavior is attributed to the dehydration of the large hydrophilic headgroup region of C22-β-Ala-DQA at high temperatures, which promotes further elongation of wormlike micelles. Surfactants with long saturated alkyl tails were used to prepare stable wormlike micellar solutions. These results provide valuable guidance for designing stable wormlike micellar systems using ultralong alkyl tails. Moreover, the proposed system shows potential for applications in fracturing fluids or other smart fluid technologies.
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Yujie Zhang,Renling Lu,Ming Chen,Shouyong Zhou,Dabing Zhang,Haifeng Han,Mingliang Zhang,Hongdeng Qiu
An alternative method for efficient synthesis of urea-functionalized silanes was proposed on the basis of an N, N’ -carbonyldiimidazole-mediated acyl-transfer reaction between various amino-containing building blocks. The employment of different parent aminosilanes and alkylamines afforded an array of urea-containing silanes, which were subsequently immobilized onto silica gel to form corresponding urea-embedded alkyl stationary phases for high-performance liquid chromatography. The different substituents on the silicon core of the derivatized silane were found to significantly influence the final chromatographic behaviors. The comparative chromatographic characterization of thus-prepared silica packings with conventional octadecyl (C18) stationary phases revealed that the urea group was beneficial to suppress silanol activity towards basic probes, as well as to increase the water-compatibility of the alkyl stationary phases. The combination of a polar urea moiety and a non-polar long alkyl chain was favorable for an enhanced steric selectivity towards shape-constrained isomers. The polarizability-sensitive feature of such stationary phases made them good candidates for efficient separation of nitro-containing polar substances.
研究领域:
分析化学
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材料科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yiran Wang,Chunyu Yang,Xi Wang,Shibing Zhang,Siyi Wang,Di Wu,Kanyasiri Rakariyatham,Jiangning Hu,Qi Zhao
In our study, ammoniated hollow mesoporous silica nanoparticles (NH 2 -HMSN) with uniform diameter and stable structure were successively prepared via SiO 2 core hard template method. Fourier transformed infrared spectroscopy revealed that amino group was effectively modified. Adsorption experiments showed that adsorption capacity of NH 2 -HMSN towards free fatty acids (FFAs) was superior to aminated mesopores or silica microspheres. Following through optimization of extraction conditions, FFAs from edible oil samples were successfully gathered by NH 2 -HMSN and showed favorable linearities (0.2–90 μg g −1 ), remarkably low limit of detections (0.03–0.15 nmol g −1 ), acceptable recoveries (85.08–96.82 %) and relatively accurate precisions (1.64–4.99 %). In comparison to existing adsorbent, NH 2 -HMSN could be successfully prepared via the chemical reaction of common raw materials under normal pressure and temperature. Furthermore, NH 2 -HMSN with hollow and mesoporous structure was more effective than the current adsorbents aimed at FFAs analysis in aspect of surface area and adsorption capacity.
研究领域:
食品科学
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材料科学
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DOI
PubMed
高影响因子
Lipids in Health and Disease
·
2024
·Vol. 23
·期 1
·1-15
·
IF 3.9
Guo XiaoYu,Zhou Jianglu,Yu Hong,Cao Han,Li Xia,Hu Qing,Yu YunQiu
Background Long-chain fatty acids (LCFAs) are involved in regulating multiple physiological processes as signalling molecules. Gas chromatography–mass spectrometry (GC–MS) is widely used to quantify LCFAs. However, current quantitative methods for LCFAs using GC–MS have demonstrated complicated issues. Psoriasis is a chronic inflammatory skin disease, and its pathogenesis may be related to the overproduction of interleukin-17A (IL-17A). Clinical efficacy of anti-IL-17A monoclonal antibody (mAb) treatment in psoriasis patients has been demonstrated. Recent studies suggest that LCFAs play varying roles in the pathogenesis of psoriasis. However, more comprehensive research is needed to illuminate the mechanism of LCFAs in psoriasis. Methods The established in situ derivatization method for analysing LCFAs with a GC–MS platform was utilized to conduct serum lipidomics analysis of healthy volunteers and psoriasis patients receiving pretherapy and posttreatment with of anti-IL-17A mAb. Imiquimod (IMQ)-treated wild type (WT) and T-cell receptor delta chain knock-out ( Tcrd −/− ) mice were used to investigate the correlation between IL-17A and abnormal changes in LCFAs in psoriasis patients. Results A rapid and sensitive in situ extraction derivatization method for quantifying LCFAs using GC–MS was established. Serum lipidomic results showed that psoriasis patients had higher levels of saturated fatty acids (SFAs) and ω-6 polyunsaturated fatty acids (PUFAs) but lower levels of monounsaturated fatty acids (MUFAs) and ω-3 PUFAs than healthy individuals, indicating impaired serum LCFA metabolism. Anti-IL-17A mAb treatment affected most of these LCFA changes. Analysis of LCFAs in IMQ-treated mice showed that LCFAs increased in the serum of WT mice, while there were no significant changes in the Tcrd −/− mice. SFAs increased in IMQ-treated WT mice, while MUFAs showed the opposite trend, and PUFAs did not change significantly. Conclusions This study presented a dependable method for quantifying LCFAs that enhanced sensitivity and reduced analysis time. The lipidomic analysis results showed that anti-IL-17A mAb not only ameliorated skin lesions in psoriasis patients but also affected abnormal LCFAs metabolism. Furthermore, the study indicated a potential correlation between IL-17A and abnormal LCFA metabolism in psoriasis patients, which was supported by the alterations in serum LCFAs observed in IMQ-treated WT and Tcrd −/− mice.
研究领域:
皮肤病学
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免疫学
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代谢组学
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DOI
PubMed
高影响因子
ACS Food Science & Technology
·
2024
·
IF 2.6
Yiting Zhu,Yinghui Feng,Jiawei Wang,Zhaoting Yuan,Yulu Miao,Tingwei Miao,Bei Gao,Lujia Zhang
The synthesis of long-chain n-3 polyunsaturated fatty acid (n-3 PUFA) triacylglycerols (TAGs) via lipase-catalyzed esterification is crucial for optimal human nutrition. However, existing lipases lack selectivity for long-chain n-3 PUFAs, hindering the efficient synthesis of highly polyunsaturated TAGs. By two-dimensional NMR and quantum chemical calculations, polyunsaturated DHA was found to have a unique curved conformation. We rationally designed the lipase MAS1 from marine Streptomyces sp. Strain W007 was based on the bent conformation of DHA, using virtual saturation mutagenesis and binding free energy calculations. Consequently, D150V with more hydrogen bonds and a lower binding free energy to DHA (−28.3820 kcal/mol) was obtained. The esterification selectivity of D150V to DHA was 2.18-fold that of the wild type, whereas its selectivity to behenic acid exhibited minimal improvement. Collectively, our findings present a significant advancement in lipase design for selective long-chain n-3 PUFA esterification, thereby opening avenues for lipase modification and industrial applications.
研究领域:
生物化学
·
酶工程
·
营养科学
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DOI
高影响因子
Biomaterials Science
·
2025
·Vol. 13
·期 5
·1272-1285
·
IF 5.7
Zhixin Lei,Xiaoli Cai,Juan Wan,Ze Li,Taolei Sun
To enhance the antibacterial efficacy of tildipirosin against Staphylococcus aureus (S.A.) infections, optimized solid lipid nanoparticles loaded with tildipirosin (SLN-TD) were developed, using docosanoic acid (DA), octadecanoic acid (OA), hexadecanoic acid (HA), and tetradecanoic acid (TA) as lipid components. The efficacy of these nanoparticles against S.A. was evaluated using orthogonal design analysis. FTIR, DLS, HPLC, and TEM analyses confirmed that tildipirosin was successfully incorporated into the solid lipid nanoparticles, resulting in an optimal nanoparticle drug delivery system with a particle size of 322.63 ± 1.51 nm, a zeta potential of 37.83 ± 0.95 mV, an encapsulation efficiency of 82.23 ± 0.45%, and a drug loading capacity of 7.36 ± 0.18%. The SLN-TD system exhibited high stability, effective sustained release in vitro, and enhanced intracellular activity against S.A. Pharmacokinetic studies in rats administered 4 mg kg−1via intramuscular and oral routes showed that, compared to unencapsulated tildipirosin (TD), SLN-TD provided sustained release in vivo and improved gastrointestinal absorption with higher bioavailability. Additionally, in a mouse model of S.A. infection, SLN-TD demonstrated superior antibacterial activity and sustained drug delivery for effective treatment. This study offers a promising multifunctional nanoparticle drug delivery system for the effective treatment of S.A. infections and enhances the oral bioavailability of tildipirosin, with potential applications in veterinary medicine.
研究领域:
药剂学
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微生物学
·
兽医学
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产品关联
DOI
PubMed
高影响因子
Nano Research
·
2024
·
IF 9.5
He Jinhua,Wang Chenhui,Liu Mingrui,Ramzan Muhammad,Long Zhiwei,Wu Xian-gang,Chen Yu,Zhong Haizheng
The potential use of large-size ZnSe quantum dots as blue emitters for display applications has greatly inspired the colloidal synthesis. Herein, we report the negative effects of side reactions of large-size ZnSe quantum dots. The side reactions between oleic acid and oleylamine generated amidation products and H 2 O, which led to the hydrolysis of Zn(OA) 2 to Zn(OH) 2 and the subsequent formation of zinc oxide (ZnO) and zinc bis[diphenylphosphinate] (Zn(DPPA) 2 ) precipitates. These side reactions resulted in the formation of a defective surface including a Se-rich surface and oxygen-related defects. Such negative effects can be overcome by adopting an etching strategy using potassium fluoride and myristic acid in combination. By overcoating a ZnS shell, blue emissive ZnSe/ZnS quantum dots with a maximum photoluminescence quantum yield of up to 91% were obtained. We further fabricated ZnSe quantum dots-based blue light-emitting diodes with an emission peak at 456 nm. The device showed a turn-on voltage of 2.7 V with a maximum external quantum efficiency of 4.2% and a maximum luminance of 1223 cd·m −2 .
研究领域:
材料科学
·
纳米技术
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产品关联
DOI
高影响因子
VIEW
·
2026
·
IF 8.1
Yihui Zhou,Xiangjie Huang,Xingxin Liu,Ling Zhang,Jie Huang,Jing Zhao,Yongqing Zhang,Jin Zhang,Yanqing Gong,Delong Zhang,Hyeon Jeong Lee
Lipid spatial heterogeneity is essential for linking metabolic regulation to cellular behavior. Yet, subcellular lipid profiling remains a challenge, as exogenous labels cannot detect lipid speciation and species diversity. Here, we developed submicron-resolution mapping of intracellular lipid elements (SMILE), a vibrational spectrometry platform based on hyperspectral stimulated Raman scattering imaging with a spectral analysis pipeline for pixel-resolved lipid profiling. SMILE enables quantitative mapping of lipid chain length and unsaturation at 300 nm resolution without the need for lipid extraction or labeling. Using SMILE, we revealed the spatial heterogeneity of lipid species and identified a distinct subset of highly aggregated lipid droplets (LDs) enriched in long-chain polyunsaturated lipids in invasive cancer cells. Lipid elongation was found to promote LD aggregation and cancer migration/invasion, thereby defining this as a unique spatial lipid-metabolic signature associated with cancer aggressiveness. Mechanistically, we identified linolenic acid as a key mediator driving aggressive lipid signatures and cancer invasion. Collectively, these findings demonstrate SMILE as an enabling spatial-omics platform that expands the chemical resolution of vibrational spectroscopic imaging for fundamental and translational research.
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Yi Xia,Jie Shi,Qian Sun,Dan Wang,Xiao-Fei Zeng,Jie-Xin Wang,Jian-Feng Chen
Colloidal nanocrystals can offer exquisite control of physical and chemical properties for technological applications by tuning their sizes, shapes and crystal forms. Herein, a convenient phase-transfer route was presented to controllably prepare transparent dispersions of monodispersed ZrO 2 nanocrystals in an aqueous-organic two-phase medium. By adjusting the surfactants, solvents and basicity, the controllability of crystal forms from monoclinic to tetragonal phases, the particle length from 5 to 20 nm, and the different shapes including spindle, cobblestone, sphere, branch, rod and cube can be achieved. The as-prepared ZrO 2 nanocrystals can be readily dispersed in nonpolar solvents, thereby forming the ultra-highly concentrated (60 wt%), highly stable (>18 months) and highly transparent nanodispersions. Density functional theory (DFT) calculations were further used to elucidate the root causes of underpinning growth mechanisms. This work not merely enriches the methodology of controllable fabrication of monodispersed sub-10 nm ZrO 2 nanocrystals, but also enables a platform to synthesize rare-earth-doped nanophosphors using ZrO 2 as the host matrix. The fluorescent ZrO 2 :Yb 3+ /Er 3+ nanocrystals, which can be easily monodispersed in organic solvents, were also prepared with visible luminescene properties under near-infrared irradiation.
研究领域:
材料科学
·
化学
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DOI
高影响因子
Materials
·
2024
·Vol. 17
·期 6
·1286
·
IF 3.1
Junlin Huang,Chih-Hung Wu,Fuying Li,Xiang Wang,Sheng-Chung Chen
The usage time of air-cathode microbial fuel cells (MFCs) is significantly influenced by the moisture content within the proton exchange membrane (PEM). Therefore, enhancing the water retention capability of the PEM by applying a hydrophobic polymer coating to its surface has extended the PEM’s usage time by three times and increased MFCs’ operational duration by 66%. Moreover, the hydrophobic nature of the polymer coating reduces contamination on the PEM and prevents anode liquid from permeating into the air cathode. Towards the end of MFC operation, the internal resistance of the MFC is reduced by 45%. The polymer coating effectively maintained the oxygen reduction reaction activity in the cathode. The polymer coating’s ability to restrict oxygen transmembrane diffusion is demonstrated by experimental data showing a significant decrease in oxygen diffusion coefficient due to its presence. The degradation efficiency of the chemical oxygen demand from 16% to 35% increased by a factor of one.
研究领域:
环境科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2019
·
IF 3.829
Yanfei Tao,Fei Yang,Kuiyu Meng,Dongmei Chen,Yujuan Yang,Kaixiang Zhou,Wanhe Luo,Wei Qu,Yuanhu Pan,Zonghui Yuan,Shuyu Xie
In our previous study, enrofloxacin-loaded docosanoic acid solid lipid nanoparticles (SLNs) could be effectively delivered to cells in vitro. In this study, its properties and exploitation as possible oral and intramuscular sustained release formulations for pigs were studied after being made into suspension. The re-dispersed time and sedimentation rate of the nanosuspension were 55 s and 1, respectively. It showed good stability when stored away from light and sustained release in pH = 7.4 PBS buffer. The suspension exhibited no irritation at the injection site and good palatability. Compared with commercial injection and soluble powder, the nanosuspension increased the bioavailability of enrofloxacin by 1.63 and 2.38 folds, and extended the mean residence time (MRT) of the drug from 11.27 and 12.33 to 37.76 and 35.15 h after intragastric and intramuscular administration, respectively. These results suggest that docosanoic acid SLN suspension (DAS) might be a promising oral and intramuscular sustained release formulation to enhance the pharmacological activity of enrofloxacin.
研究领域:
兽医学
·
药物递送系统
·
药剂学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）