JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Liling Wang,Yanbin Wang,Yuchuan Qin,Liang He
U.S. pecan nuts are rich in oil and phenolic compounds with important biological functions. At present, the reports about simultaneous extraction of oil and phenolic compounds are limited, and the traditional procedure is complicated and the yield is low. In this study, a new emulsion formed by hydrophobic deep eutectic solvent (DES) and water was used as extractant for synchronous extraction of oil and phenolic components from U.S. pecans, and subsequently the two were recovered from the upper and lower phases based on the incompatibility of DES and water, respectively. The oil and phenolic compounds obtained by the optimization of extraction conditions were 615.29 mg/g and 82.11 mg/g, respectively, which were higher than those obtained by conventional methods. At the same time, the extraction mechanism is discussed through the extraction kinetics and thermodynamics. The extraction process conformed to the Fick’s second law, and it was an endothermic process. After directional recovery by macroporous resin and back-extraction, the effectiveness of the method was proved by the analysis of fatty acid composition of oil, spectral characterization of phenolic compounds and antioxidant activities.
研究领域:
食品科学
·
植物化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Fei Hu,Ao-Te Ci,Hao Wang,Yuan-Yuan Zhang,Jian-Guo Zhang,Kiran Thakur,Zhao-Jun Wei
In this study, the hydrolysis of a pecan protein isolate (PPI) with Alcalase was carried out to generate antioxidant peptides. We proposed a kinetic model to illustrate the enzymolysis process of PPI, which was found suitable for depiction of the kinetic behavior for PPI hydrolysis by Alcalase. The PPI hydrolysis products were gradually fractionated by ultrafiltration through cut-off membranes with molecular weights of 10, 5 and 3 kDa and their antioxidant activities were evaluated in vitro . Further, the strongest antioxidant fraction (<3 kDa) and novel antioxidative peptide were successfully purified. The amino acid sequence of the purified peptide was identified as LAYLQYTDFETR. The purified fraction exhibited appreciable scavenging activities on ABTS radical (67.67%), DPPH radical (56.25%) and hydroxyl radical (47.42%) at 0.1 mg/mL. The results suggested that this novel peptide may serve as a potential antioxidant and it should be evaluated for development of functional foods and pharmaceuticals products.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
PLANT DISEASE
·
2019
·Vol. 103
·期 12
·
IF 3.583
Q. W. Wang,C. Q. Zhang
Trunk canker disease caused by Botryosphaeria dothidea with a prolonged latent infection phase poses a serious threat to Chinese hickory production. To further understand the epidemiological characteristics and develop reasonable management techniques, a quantitative loop-mediated isothermal amplification (q-LAMP) assay was developed to quantitatively monitor B. dothidea in hickory plants, water, and air samples. Specific primers were designed based on the different sites of the β-tubulin sequence between B. dothidea and other fungi commonly found on Chinese hickory. At the optimum reaction temperature of 65.9°C, this loop-mediated isothermal amplification (LAMP) assay can specifically distinguish B. dothidea from other tested fungi. The limit of detection of LAMP assays for B. dothidea was 0.001 ng/µl of pure genomic DNA and 10 spores per 1 ml of water. The q-LAMP assay enables rapid detection of B. dothidea within 60 min in hickory trunk, water in hickory forests, and spores captured on tapes. These results provide a powerful and convenient tool for monitoring B. dothidea , which could be applied widely in epidemiology, forecast, and management of tree canker disease.
研究领域:
植物病理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2016
·
IF 3.973
Limin Xiang,Yihai Wang,Xiaomin Yi,Xinluan Wang,Xiangjiu He
Chinese hickory ( Carya cathayensis Sarg.) is a common and nutritious nut. Twenty-nine phenolic compounds, together with three quinones and three triterpenoids , were isolated from Chinese hickory husks. Their structures were determined by comprehensive spectroscopic methods. The total methanolic extract, chloroform and ethyl acetate fractions and all the isolated compounds were evaluated for antioxidant activity and neuroprotective effects on SH-SY5Y cells, as well as the effects on the expression of PPAR-γ in 3T3-L1 cells. Quercetin ( 10 ) and protocatechuic acid methyl ester ( 24 ) showed pronounced antioxidant activities in all of the different antioxidant assays and neuroprotective effect against H 2 O 2 -induced oxidative stress . Some of the flavonoids could significantly decrease the mRNA expression level of PPAR-γ by approximately 70%. The present study provided supplement information for the evaluation of antioxidant activity and health benefit of Chinese hickory.
研究领域:
植物化学
·
药理学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
BaoZhu Shi,Haixin Sun,Qiaojun Jia,Hao Zhang,Hao Huang,Zisheng Luo
Carya cathayensis Sarg. green husk (CSGH), one kind of by-product in the nut industry, contains abundant triterpenic acids. This study explores a green extraction approach of triterpenic acid from CSGH using nanobubbles (NBs) and ultrasound-assisted extraction based on hydrophobic natural deep eutectic solvents (HBDES). Triterpenic acids were identified in CSGH using UPLC-Triple-TOF/MS. The HBDES was designed via Hansen solubility parameters, consisting of thymol and linalool (Thy/Lin), and demonstrated the highest yield of triterpenic acids. NBs were generated in Thy/Lin, with an average size of 237.36 nm and the triterpenic acids yield enhancement. Ultrasonic conditions also affect the total contents of the triterpenic acids extracted by Thy/Lin containing NBs partially due to the characters of NBs under ultrasound. The microstructural changes of CSGH confirmed the synergistic effect between ultrasound and NBs which enhanced the release of triterpenic acids. This proposed technique offers a green and efficient solution for extracting triterpenic acids.
研究领域:
食品科学
·
环境科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 24
·4425
·
IF 5.2
Xiaosan Zhang,Guangrong Huang,Hua Liu,Wenwei Chen,Jing Zhao,Zhenbao Jia,Fei Tao
Inhibiting α-amylase can lower postprandial blood glucose levels and delay glucose absorption, offering an effective approach for the development of antidiabetic diets. In this study, an active constituent with inhibitory activity against α-amylase was isolated and purified by bioassay-guided fractionation fromCarya cathayensisSarg. peel (CCSP). The active constituent was identified by NMR and Q-Exactive Orbitrap Mass Spectrometry as 5-O-p-coumaroylquinic acid (5-CQA). 5-CQA possessed strong inhibitory activity against α-amylase, with an IC50 value of 69.39 µM. In addition, the results of the kinetic study indicated that 5-CQA was a potent, reversible, noncompetitive inhibitor against α-amylase. The findings indicate that 5-CQA derived from CCSP has potential as a novel inhibitor against α-amylase, which can help mitigate postprandial blood sugar spikes, making it suitable for inclusion in antidiabetic diets.Keywords:Carya cathayensisSarg. Peel;postprandial hyperglycemia;5-O-p-coumaroylquinic acid;α-amylase inhibitor
研究领域:
植物化学
·
代谢病学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 46
·30145-30156
·
IF 4.036
Haoming Ning,Zhi Zhang,Chunhai Shi,Xiaolei Ma,Jian Li,Hongyi Zhu,Jiawei Hu
In this study, Fe/N codoped porous graphitic carbon derived from macadamia shells was prepared at different temperatures as cathodic catalysts for microbial fuel cells (MFCs), with K2FeO4 as a bifunctional catalyst for porosity and graphitization. The catalyst prepared at 750 °C (referred to as MSAC-750) showed a large specific surface area (1670.3 m2 g−1), graphite structure, and high pyridine-N and Fe-NX contents. Through the electrochemical workstation test, MSAC-750 shows excellent oxygen reduction reaction (ORR) activity, with an onset potential of 0.172 V and a half-wave potential of −0.028 V (vs. Ag/AgCl) in a neutral medium, and the ORR electron transfer number is 3.89. When applied to the MFCs as cathodic catalysts, a higher maximum power density and voltage of 378.68 mW m−2 and 0.425 V were achieved with the MSAC-750 catalyst and is superior to that of the Pt/C catalyst (300.85 mW m−2 and 0.402 V). In this case, a promising method is hereby established for the preparation of an excellent electrochemical catalyst for microbial fuel cells using inexpensive and easily available macadamia shells.
研究领域:
材料科学
·
环境工程
·
能源科学
✓ 已验证
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Song Wang,Chenyu Jiang,Shitong Yu,Qianru Fan,Kai Yang,Jianqin Huang,Yan Li
Background Hickory ( Carya cathayensis ) nuts, renowned for their health benefits and delightful taste, contain abundant bioactive compounds, particularly polyphenols. However, the specific mechanisms underlying their antioxidant properties and anti-aging effects remain elusive. Purpose This study aims to investigate the effects of hickory nut polyphenols (HNP) on oxidative stress mitigation and aging modulation. Methods The innovative integration of medium-pressure liquid chromatography (MPLC) with in vitro bioactive screening was employed to discover an optimized HNP fraction (HNP3–2). Anti-aging effects of HNP3–2 on Caenorhabditis elegans ( C. elegans ) were determined through lifespan analysis, lipofuscin accumulation quantification, and motility assessments. Oxidative stress resistance was further evaluated by detecting the reactive oxygen species (ROS) contents, lipid peroxidation, and antioxidase activities. Integrative approaches combining transcriptomic, qRT-PCR, GFP reporter strains, and gene knockout mutants were utilized to explore the potential regulatory mechanisms. Non-targeted metabolomics was employed to conduct a comprehensive profiling analysis of HNP and their bioactive fractions. Results HNP3–2 significantly decreased ROS production, lipofuscin accumulation, and lipid peroxidation, while enhanced the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). It also conferred robust protection against oxidative stress induced by H 2 O 2 and juglone. HNP3–2 regulated lifespan extension mainly via the DAF-16/-2 insulin/IGF-1 signaling (IIS), which was further validated using loss- and gain-of-function mutants including age-1, akt-1/2, daf-2 , and daf-16 , as well as worms overexpressing SOD-3, GST-4, and DAF-16. Metabolomic analysis identified 31 kinds of polyphenol compounds displaying abundance patterns congruent with the overall antioxidant potency in vitro. Conclusion This study first reveals the efficacy of HNP as a promising antioxidant and anti-aging intervention. Notably, the screened bioactive fraction HNP3–2 acts primarily by modulating the DAF-16/DAF-2 insulin/IGF-1 signaling cascade, operating through a mechanism independent of dietary restriction. Collectively, these findings position HNP as a breakthrough candidate for next-generation gerotherapeutics, with translational potential for advancing human aging research.
研究领域:
生物化学
·
分子生物学
·
衰老研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2016
·
IF 2.711
Peng Hu,Xuebing Xu,Liangli Lucy Yu
This study obtained trans -free fats containing nervonic acid, a fatty acid with beneficial effects for mental health. Four fats containing 1.28–2.41% (w/w) nervonic acid were prepared by chemical or enzymatic interesterification of palm stearin (PS), Acer truncatum oil (ATO) and palm kernel oil (PKO). The chemical procedure was catalyzed using MeONa, whereas the enzymatic procedure was catalyzed with a 1,3–specific lipase. As a result, the interesterified fats showed a similar fatty acid profile to their corresponding physical blends measured by gas chromatography , but contained more nervonic acids at the sn -2 position, which was more conducive to metabolism and absorption. The content of tripalmitoylglycerol (PPP) and 1,3-dipalmitoyl-2-oleoyl glycerol (POP) or 1,2-dipalmitoyl-3-oleoyl glycerol (PPO) in the interesterified fats decreased significantly, resulting in improved plasticities. All interesterified fats showed a satisfactory content of tocopherols and oxidative stability. A combination of differential scanning calorimetry , X-ray diffraction spectroscopy and polarized light microscopy results suggested that the interesterified fats formed spherulitic needle-like crystals in the β′ form, while the physical blends formed dendritic crystals in the β form. These data indicated that the trans -free interesterified fats rich in nervonic acid may have a great potential in margarine applications.
研究领域:
食品科学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xizhe Fu,Tarun Belwal,Yihan He,Yanqun Xu,Li Li,Zisheng Luo
Natural deep eutectic solvent (NADES) has received increasing interest as a green alternative to traditional organic solvents for efficient extraction of bioactive compounds from natural sources. In this study, phytochemicals in Carya cathayensis Sarg. peels extracted with Choline chloride-Malic acid (ChCl-MA) were identified using UPLC-Triple-TOF/MS. Effect of NADES on phenolic composition, antioxidant properties and inhibition of α-glucosidase and α-amylase were evaluated. Furthermore, extraction mechanism caused by different solvents were investigated by quantum chemical calculation combined with molecular dynamic simulation. A total of 29 phytochemicals were identified, and catechin, procyanidin B1, 2,3-dihydroxybenzoic acid, pinocembrin, procyanidin B3, myricetrin were the most abundant compounds. The extract using ChCl-MA exhibited the highest phenolic compounds content, antioxidant capacity, and α-glucosidase and α-amylase inhibition activities. Larger solvent accessible surface area, more hydrogen bonds between ChCl-MA and extract, longer lifetime of the hydrogen bonds, and lower intermolecular interaction energy account for higher extraction efficiency of ChCl-MA.
研究领域:
植物化学
·
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）