Current Research in Food Science
·
2024
·
IF 6.2
Jiayue Lou,Baojie Zhang,Yu Zheng,Meiqi Liu,Yang Qu
ABSTRACT Studies have shown that there is a close relationship between acute myocardial ischaemia (AMI) and intestinal flora imbalance. And pectin has a protective effect on AMI and regulates intestinal flora. Raw hawthorn pectin from hawthorn (RHP) is high methoxyl pectin, which is able to protect injury induced by AMI. After stir-frying of hawthorn, pectin from stir-fried hawthorn (FHP) transformed to low methoxyl pectin, the protective mechanisms against AMI is not well-understood. In this study, the protective effects of RHP and FHP against AMI rats were explored. The results revealed that FHP regulated myocardial enzymes including CK, CK-MB and CTn-1, oxidative stress-related indicator SOD more significantly than RHP. According to the determination of proportion of different kinds of short-chain fatty acids (SCFAs) and abundance of microbiota producing SCFAs, it was speculated that RHP and FHP were fermented by these microbiota. RHP increased the proportion of acetic acid and butyric acid, while FHP increased the proportion of acetic acid in feces. Pretreatment with RHP and FHP enriched the beneficial microbiota and maintained the levels of SCFAs, which significantly increased after modeling. These results revealed that RHP and FHP played a protective role in myocardial ischaemia by regulating intestinal flora and SCFAs.
研究领域:
心脏病学
·
胃肠病学
·
营养学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Food Quality and Safety
·
2024
·
IF 3
Liu Yilong,You Wenwen,Wang Ruoqi,Zeng Tao,Zhao Xiaoyong,Cao Yunlin,Zhang Wenna,Nie Zongxing,Yang Xiaochun,Li Xian
Hawthorn (Crataegus spp.) is a functional food with long medical usage and excellent health-promoting effects. In this study, comparative metabolomics analysis between the yellow hawthorn berry (Crataegus pinnatifida) and the red-skinned ones was first carried out with the peel and flesh separated to fully understand the polyphenol composition and metabolite variations. A total of 453 polyphenols was characterized, among which phenolic acids and flavonoids were abundant, and were closely relevant to the antioxidant capacity of hawthorn fruits. Polyphenol profile showed accession-specific accumulation in peel or flesh of different colored hawthorn. The unique yellow hawthorn was found to have few anthocyanins but show enhanced flux to synthesize flavones and flavonols, especially flavone C-glycosides and acylated flavonol glycosides. The specific acylation decoration includes acetylation, p-coumaroylation, and malonylation. In addition, the yellow hawthorn showed excellent α-glucosidase inhibitory effect, which might be associated with the high concentration of 8 polyphenols including 5 phenolic acids, 2 flavone C-glycosides, and an acylated flavonol glycoside, namely quercetin-7-O-(6''-malonyl)glucoside. Such acylated flavonol showed the highest correlation ship with the inhibition effect of hawthorn fruits on α-glucosidase, and was predicted to had the lowest binding energy with the enzyme by molecular docking analysis, indicating its great potential as a strong α-glucosidase inhibitors and an important antidiabetic ingredient in yellow hawthorn. Thus, acylated flavonol glycosides and C-glycosyl flavones might be chemotaxonomic markers differentiating varieties and bioactivities of yellow hawthorn from the traditional red-skinned ones. These findings complement the existing knowledge on the metabolite composition and nutritional properties of hawthorn fruits.
研究领域:
食品科学
·
代谢组学
·
营养学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 23
·4328
·
IF 3.06
Hongyu Rao,Peibo Li,Hao Wu,Chong Liu,Wei Peng,Weiwei Su
Hawthorn seed can be used to produce various bioactive compounds through destructive distillation. In this study, an accurate and feasible analytical method based on a gas chromatography mass spectrometer (GC-MS) was developed for simultaneous determination of six major compounds (contributing to more than 3% in total peak area) in destructive distillation extracts of hawthorn seed collected at different temperatures ranging from 150 to 270 °C. Then, a broth microdilution method coupled with grey correlation analysis was engaged in the evaluation of their antimicrobial activities and the screening of primarily active compounds. Results indicate that the extract collected from 211 to 230 °C had the highest content of six major compounds (furfural, 2-methoxyphenol, 2-methoxy-4-methylphenol, 4-ethyl-2-methoxyphenol, 2,6-dimethoxyphenol, and 5-tertbutylpyrogallol) and the strongest antibacterial activity. Besides, 2,6-dimethoxyphenol was found to be a potential compound in inhibiting the growth of vaginitis pathogens. This study provided an optimum temperature for the destructive distillation of hawthorn seed, reducing the waste of energy, and saving the cost of production in the hawthorn industry.Keywords:hawthorn seed;GC-MS;phenolic compounds;antimicrobial activity
研究领域:
植物化学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Shihai Zhang,Chunqian Zhang,Meixia Li,Xia Chen,Kan Ding
Crataegus pinnatifida is widely used as functional food and traditional medicine in China for various applications. Polysaccharides may contribute to health benefits, such as modulating human gut microbiota (HGM). To investigate structure features of polysaccharides from this plant, fruits of C. pinnatifida were extracted with boiling water, yielding crude polysaccharides HAW. A novel polysaccharide, named HAW1-1, was pooled by DEAE Sepharose™ Fast Flow column and further purified by Sephacryl S-100 HR column. The molecular weight of HAW1-1 was 42.35 kDa. The carbohydrate content of HAW1-1 was 99.42% and no protein was detected. The monosaccharide analysis showed this polysaccharide was only composed of glucose. According to the partial acid hydrolysis, methylation analysis and NMR spectra, the backbone of HAW1-1 was consisted of 1,4-linked α-D-glucan and 1,4,6-linked α-D-glucan. The side chains were composed of 1,6-linked α-D-glucan and terminal-linked α-D-glucan. The bioactivity tests showed that HAW1-1 could promote the growth of three species of intestinal Bacteroides and produce short-chain fatty acids (SCFAs). The results suggested that HAW1-1 might be a potential prebiotic candidate to maintain intestinal homeostasis and improve human gut health.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 11
·2150
·
IF 3.06
Yingying Han,Jinhua Du,Jie Li,Miaomiao Li
Hawthorn wine is rich in anthocyanins, polyphenols, flavonoids and other macromolecular substances, which results in difficulty to rapidly determine organic acids in the wine. An enzymatic method is accurate but expensive and not able to quantify all of the organic acids simultaneously. Therefore, in this study, two HPLC methods were applied to quantify the organic acids in the wine with the enzymatic method as a reference. Seven organic acids were found with the enzymatic method including citric, succinic,l-malic, acetic, lactic, pyruvic, and fumaric acids, in which citric and succinic acid accounted for more than 80% of the total acids. By an 87H column equipped with DAD (diode array) detector at 215 nm (HPLC method 1), only citric and lactic acids were quantified accurately and the elution period was shortened from 100 min to 20 min by removing the impurity in the sample with a LC-18 SPE(solid-phase extraction) tube. While citric, succinic,l-malic, acetic, pyruvic, and fumaric acids were quantified reliably by a dC18 column equipped with DAD detector at 210 nm (HPLC method 2), with the sample requires only dilution and filtration before injection. It was suggested that HPLC method 2 was an effective method to quantify the organic acids in hawthorn wine. The method provides a choice for accurate quantification of organic acids in hawthorn wine or other drinks, and would be helpful for controlling the quality of hawthorn wine.Keywords:hawthorn wine;organic acids;high-performance liquid chromatography methods;enzymatic method
研究领域:
食品化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Qianyun Zhao,Yubin Liu,Mingxing Li,Long Zhao,Tiangang Wang,Yaqin Xiao,Shulin Wei,Ke Wu,Jiayue Yang,Yaqiu Wang,Yueshui Zhao,Fukuan Du,Yu Chen,Shuai Deng,Jing Shen,Zhangang Xiao,Zhi Li,Shengpeng Wang,Xu Wu
Hawthorn fruit is rich in pectins; however, the gut microbiota-modulating effects of hawthorn pectin remain unclear. In this study, enzyme-extracted hawthorn pectin ( M w : 126.24 kDa; HG/RG-I ratio: 55.64 %/37.93 %; branching degree: 2.44) was fractionated into two components: D1 (spherical, 345.76 kDa, HG/RG-I 57.48 %/37.25 %, branching degree 2.16) and D2 (flexible, 17.20 kDa, HG/RG-I 78.24 %/21.76 %, branching degree 1.33). Hawthorn pectin directly stimulated Dubosiella newyorkensis and Bifidobacterium bifidum growth in vitro . In hyperlipidemic mice, it alleviated dyslipidemia and enriched Lactobacillus and Dubosiella . Parallel experiments confirmed that D. newyorkensis supplementation similarly reduced dyslipidemia and enhanced Lactobacillus abundance, mediated by bacterial acetate production. Only D1 mimicked the pectin's prebiotic activity, promoting D. newyorkensis and B. bifidum growth. The high-RG-I D1, with elevated branching and larger M w , was identified as the key driver of hawthorn pectin's prebiotic effect. These findings highlight that hawthorn pectin and D1 specifically target probiotic D. newyorkensis to synergize with Lactobacillus against hyperlipidemia.
研究领域:
营养学
·
微生物学
·
代谢疾病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·
IF 3.2
Ying Yuan,Ziqi Chai,Yuxue Zheng,Yanming Ren,Xingqian Ye,Xiangli Kong,Jinhu Tian
To investigate the effect of hawthorn polyphenols on the physicochemical properties and digestibility of corn starch, different proportions (1%, 2%, 3%, and 4% [w/w]) of hawthorn polyphenol extracts (HPEs) were mixed with corn starch, and their physicochemical properties and digestive properties were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, Rapid Visco Analysis, differential scanning calorimetry, and in vitro/in vivo analysis. Results indicated that small V-type crystal starch tended to be formed in the samples, and the addition of HPEs reduced the viscosity, prolonged the gelatinization temperature of corn starch, and increased the proportion of slowly digestible starch and resistant starch of the corn starch, which accounted for 36.32% ± 1.05% and 33.32% ± 4.07%, respectively. Compared with the raw corn starch, the postprandial blood glucose of mice that were administered the hawthorn polyphenols decreased significantly: the blood glucose peak (30 min) decreased from 14.30 ± 1.52 to 11.77 ± 1.21 mmol/L. Our study might provide some basic theoretical support for the application of hawthorn polyphenols in healthy starchy food processing.
研究领域:
食品科学
·
营养学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ran Zhang,Lulu Xing,Xin Wang,Zuoyu Shan,Tianxin Wang,Ye Zhang,Wenjie Wang,Yuan Wang,Hao Wang
Inhibiting the activity of pancreatic lipase and reducing intestinal cholesterol absorption are potential strategies to combat obesity. This study investigated the mechanisms by which hawthorn ( Crataegus pinnatifida ) extract affects pancreatic lipase (PL) and disrupts cholesterol micelle formation. Enriched with bioactive compounds, hawthorn extract (HE) inhibited PL activity through reversible mixed inhibition, with a half-maximal inhibitory concentration (IC50) of 2.92 mg/mL. Infrared spectroscopy, circular dichroism and fluorescence quenching experiments demonstrated that HE binding to PL induces conformational changes in both tertiary and secondary structures. This interaction facilitated the transformation of β-turns to random coils and quenched the fluorescence of the protein through a static quenching mechanism. HPLC, immobilized enzymes and molecular docking studies collectively revealed that rutin, chlorogenic acid, and isoquercitrin in HE exhibited strong binding affinity with PL, serving as key components in inhibiting PL activity. Furthermore, HE increased the particle size of cholesterol micelles while decreasing their solubility, which makes it more difficult for lipases to function in the intestine. Overall, our study suggests that HE may serve as an effective pancreatic lipase inhibitor, presenting potential applications in the development of functional foods for obesity reduction and lipid-lowering.
研究领域:
营养学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Xiaojing Sun,Shihao Lou,Di Zhang,Dandan Li,Jie Zhou,Hong Zhu,Chenchen Zhao,Lirong Wang,Junfang Zhu,Lingjia Chen,Xue Han,Dehua Mou,Jianhua Xiu
In view of the possible synergistic effects of the combination of polyphenols and proteins, the present study investigated the mode of interaction between hawthorn flavonoids (HF) and soybean isolated protein (SPI). The FTIR analysis demonstrated that the amino group of the amide in SPI interacted with the hydroxyl group in HF, forming hydrogen bonds. SEM showed that HF changed the microstructure and surface morphology of SPI.XRD diffraction showed that part of the crystal structure of SPI changed to the amorphous structure. Furthermore, molecular docking and molecular dynamics simulations indicate that the binding energy of HF and SPI 7S is predominantly van der Waals energy(−37.66 kcal/mol), and Electrostatic energy is −0.74 kcal/mol. The primary factor governing the binding energy between HF and SPI 11S is the van der Waals energy(−45.11 kcal/mol), The electrostatic energy is −25.6 kcal/mol. Briefly, the binding between HF and SPI in this study is non-covalent.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2021
·Vol. 35
·期 6
·e5076
·
IF 1.902
Kaiyang Wang,Lan Luo,Xiaoli Xu,Xingyu Chen,Qiong He,Zhongjie Zou,Shumei Wang,Shengwang Liang
Dyspepsia, one of the most prevalent diseases of the digestive tract that impacts the quality of patient life, is mainly caused by gastrointestinal motility disorder. Hawthorn is a commonly used traditional Chinese medicine for treating dyspepsia, and has been proven to improve gastrointestinal motility. Herein, a rat model of gastrointestinal motility disorder was established by subcutaneous injection with atropine. The modeled rats were treated with four polar parts (T1–4 in descending polarity, corresponding to water, n -butanol, ethyl acetate and petroleum ether extracts, respectively) of hawthorn. Through metabolomics analysis, a total of 20 significantly metabolites were identified with significant changes in their abundance levels and these metabolites were related to many metabolic pathways such as amino acid metabolism and primary bile acid biosynthesis. The results showed that T3 had the best therapeutic effect of promoting gastrointestinal motility. Other parts showed no obvious therapeutic effect, demonstrating that the effective components of hawthorn may be compounds of medium polarity. T3 might achieve good therapeutic effects owing to the gastrointestinal motility promotion activity, and by rectifying the disturbed metabolic pathways in the gastrointestinal motility disorder model.
研究领域:
药理学
·
代谢组学
·
传统中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Shuai-Shuai Hu,Ling Yi,Xing-Ying Li,Jun Cao,Li-Hong Ye,Wan Cao,Jian-Hua Da,Han-Bin Dai,Xiao-Juan Liu
An ionic liquid (IL)-based one-step micellar extraction procedure was developed for the extraction of multiclass polar analytes (protocatechuic acid, chlorogenic acid, epicatechin, hyperoside, isoquercitrin, quercetin) from hawthorn fruits and their determination using ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS). Compared to conventional organic solvent extractions, this newly proposed method was much easier, more sensitive, environmentally friendly, and effective as well. Several important parameters influencing the micellar extraction efficiency are discussed, such as selection of ILs, surfactant concentration, and extraction time. Under the optimal conditions, good linearity was achieved for each analyte with correlation coefficients (r2) ranging from 0.9934 to 0.9999, and the recovery values ranged from 89.3 to 106% with relative standard deviations lower than 5.5%. Results suggest that the IL-based one-step micellar extraction could be an alternative and promising means in future food analysis.
研究领域:
分析化学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
Food Chemistry-X
·
2022
·
IF 6.443
Ziqi Chai,Xiuxiu Yin,Yuxue Zheng,Xingqian Ye,Jinhu Tian
Hawthorn powder were mixed with corn starch and heated in water to make corn starch-hawthorn mixtures (CS-Haw) and then the physicochemical properties and hydrolysis characteristics of the mixtures were measured. Results showed that the addition of hawthorn powder decreased the viscosity of corn starch, and prolonged the pasting temperature, while the microstructure analysis indicated that hawthorn particles aggregated on the surfaces of starch granules, reducing the chance of starch contacting with water, then delayed the starch gelatinization. The presence of hawthorn powder also reduced the G' value to varying degrees and the loss tangent of CS-Haw was significantly higher than that of corn starch. The addition of hawthorn powder in large amounts also increased the rapidly digestible starch, while decrease the slowly digestible starch and resistant starch. The present research will provide basic theoretical support for the application of hawthorn in healthy starch food processing.
研究领域:
食品科学
·
植物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）