LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Mengting Lai,Hongzhu Chen,Xiaozhen Liu,Fuxiang Li,Fengyuan Liu,Yuting Li,Jingkun Yan,Li Lin
The aim of this study was to reveal the phenolic profile of mangosteen ( Garcinia mangostana L. ) pericarp and investigate their inhibitory effects on α-amylase and α-glucosidase. Mangosteen pericarp polyphenols (MPP) obtained by acetone extraction were fractionated with gradient ethanol elution. A total of twenty nine phenolic chemicals were found and quantified by UPLC-Q-TOF-MS/MS. MPP eluted by the 60% ethanol solution (MPP 60% ) showed the highest total phenolic content. Moreover, MPP 60% displayed the greatest inhibition activities on α-amylase and α-glycosidase by competitive and mixed inhibitions. Mechanistically, the inhibitory effects of MPP 60% on α-amylase and α-glucosidase involved the changes of the secondary structure as well as the static fluorescence quenching of these enzymes. Pearson correlation coefficients revealed that proanthocyanidin B2, quercetin glucoside, and mangostain contributed most to inhibiting the activities of α-amylase and α-glucosidase. This study indicated that polyphenols from mangosteen pericarps can be developed into potential inhibitors of α-amylase and α-glucosidase, offering a viable strategy for the valorization of mangosteen pericarps.
研究领域:
食品科学
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药理学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2021
·Vol. 22
·期 19
·10365
·
IF 5.924
Shan Jin,Wen Wang,Fei Gan,Wenli Xie,Jing Xu,Yu Chen,Zhinan Mei,Guangzhong Yang
Pharmacologic studies have revealed that polycyclic polyprenylated acylphloroglucinols (PPAPs) collectively exhibit a broad range of biological activities, including antineoplastic potential. Here, six new PPAPs, named garcixanthochymones F–K (3,5,7,8,11,and15), together with nine known analogues were isolated from the fruits ofGarcinia xanthochymus. Their structures were elucidated based on the spectroscopic data, including UV, HRESIMS, and NMR, and quantum chemical calculations. All the isolated PPAPs were tested for anti-proliferative activity against four human tumor cell lines, including SGC7901, A549, HepG2, and MCF-7. Most of the PPAPs possessed high anti-proliferative activity with IC50values in the range of 0.89 to 36.98 μM, and significant apoptosis was observed in MCF-7 cells exposed to compounds2and5. Besides, docking results showed that compounds2and5could strongly combine with the Src homology 2 (SH2) domain of STAT3 via hydrogen bond and hydrophobic interaction, which is one of the key oncogenes and crucial therapeutic targets. Furthermore, compounds2and5efficiently downregulated the expression of p-STAT3Tyr705and pivotal effector proteins involved in oncogenic signaling pathways of MCF-7 cells.Keywords:polycyclic polyprenylated acylphloroglucinols;Garcinia xanthochymus;anti-proliferative;STAT3;apoptosis
研究领域:
药理学
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肿瘤学
·
化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Wanting Fu,Zhuanzhuan Shi,Yunpeng Li,Xinyu Lou,Xiaoshuai Wu,Wei Sun,Chang Ming Li
Abnormal concentration of Dopamine (DA), a vital neurotransmitter in the nervous system, implicates the pathogenesis of various neurological disorders. However, detection of low concentrations of DA in biological samples still faces great challenge. In this work, an electrochemical DA sensor with ultra-low detection limit was innovated with mangosteen biomass carbon (MBC). Different calcination processes were applied to treat MBC, among which a unique multi-step calcination was used to sequentially heat MBC to 200 °C, 300 °C, 400 °C, and 900 °C, resulting in a unique porous 3D honeycomb structure, a large surface area, and abundant active sites. The multi-step calcinated biocarbon-based sensor offers the high sensitivity of 308 μA·μM −1 ·cm −2 , wide detection range of 0.01–300 μM, and the lowest detection limit of 2.8 nM among the best of biomass carbon-based DA sensors. This work reveals that a delicately designed calcination process plays a critical role in the electrocatalytic performance of biomass carbon. It also demonstrates that the MBC sensor is applicable for the actual detection of human serum samples, with recovery rates of 97.0 %–103.9 %, showing significant potential in biomedical applications.
研究领域:
生物医学工程
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材料科学
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神经科学
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产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2020
·
IF 3.693
Chen Mei-jun,Yang Hai-lin,Si Ya-min,Tang Qian,Chow Cheuk-fai,Gong Cheng-bin
As a moderately toxic organophosphorus pesticide, profenofos (PFF) is widely used in agricultural practice, resulting in the accumulation of a high amount of PFF in agricultural products and the environment. This will inevitably damage our health. Therefore, it is important to establish a convenient and sensitive method for the detection of PFF. This paper reports a photoresponsive surface-imprinted polymer based on poly(styrene-co-methyl acrylic acid) (PS-co-PMAA@PSMIPs) for the detection of PFF by using carboxyl-capped polystyrene microspheres (PS-co-PMAA), PFF, 4-((4-(methacryloyloxy)phenyl)diazenyl) benzoic acid, and triethanolamine trimethacrylate as the substrate, template, functional monomer, and cross-linker, respectively. PS-co-PMAA@PSMIP shows good photoresponsive properties in DMSO/H2O (3:1, v/v). Its photoisomerization rate constant exhibits a good linear relationship with PFF concentration in the range of 0~15 μmol/L. PS-co-PMAA@PSMIP was applied for the determination of PFF in spiked tomato and mangosteen with good recoveries ranging in 94.4–102.4%.
研究领域:
分析化学
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环境科学
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农药残留检测
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
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2018
·Vol. 53
·期 15
·11062-11077
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IF 2.993
Xue Mingzhe,Chen Chen,Tan Yan,Ren Zhiwei,Li Bing,Zhang Cunman
Porous carbon with various structures from micropores (< 2 nm) to hierarchical pores (0–4 nm) was synthesized from biomass waste mangosteen peels via KOH activation process. Microporous carbon was obtained mainly at a low activation temperature of 700 °C or a low alkali/carbon of 3. Hierarchical porous carbon with ultrahigh surface area (> 3000 m 2 g −1 ) and pore volume (> 1.4 cm 3 g −1 ) was obtained at alkali/carbon ratio higher than 3.5 and activation temperature of 800 °C. Sulfur with a high content of 65 wt% was able to be fully accommodated into the pores of hierarchical porous carbon to form C/S composite cathode for lithium sulfur battery while in the case of microporous carbon, residual sulfur on carbon surface was detected. High specific discharge capacities of more than 800 mAh g −1 were achieved from hierarchical porous carbon/S composites at 0.5 C, and the sample synthesized at alkali/carbon of 4 and activation of 800 °C exhibits the best cycling performance. After 500 cycles at 0.5 C, a high discharge capacity of 509 mAh g −1 was maintained, indicating an excellent capacity retention. Relationship between pore characteristics of porous carbon and the electrochemical performance of its sulfur composite was discussed.
研究领域:
材料科学
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电化学
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能源存储
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纳米材料
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可持续材料
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电池技术
✓ 已验证
DOI
高影响因子
PROCESS BIOCHEMISTRY
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2020
·
IF 2.952
Chong Ou-Yang,Weiming Chai,Xiaohui Xu,Shuang Song,Qiming Wei,Qian Huang,Zhengrong Zou
Inhibiting the activity of α-glucosidase and glycosylation of protein is an important way to treat diabetes mellitus and its complications. In this study, we investigated the anti-α-glucosidase activity, anti-glycation potential and structure of proanthocyanidins from fruit pulp of Clausena lansium (Lour.) Skeels. C. lansium fruit pulp proanthocyanidins showed a remarkable inhibition against α-glucosidase activity with IC 50 vaule of 0.26 ± 0.01 μg/mL in a competitive manner, and quenched the fluorescence of α-glucosidase by forming proanthocyanidin-α-glucosidase complex. Furthermore, compared to positive agent aminoguanidine (AG), the proanthocyanidins were the more significant inhibitors of non-enzymatic glycation by strongly inhibiting the formation of α-dicarbonyl compounds and advanced glycation end products. In addition, the structure of the proanthocyanidins was characterized in detail. These compounds were mainly composed of prodelphinidins, and their gallates. The main extender units, gallocatechin epigallocatechin and their gallates, were critical factor of the strong anti-α-glucosidase and anti-glycation activity. Therefore, this study authenticated a efficient α-glucosidase inhibitors and antiglycation agents, which would contribute to the development of anti-diabetic drug.
研究领域:
药理学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Nutrition
·
2024
·
IF 4
Yanchi Zhou,Jingtao Cui,Qiaozhu Wei,Long Wu,Tian Li,Weimin Zhang
Background: Aroma significantly influences the quality of rambutan seed oil. Nonetheless, few studies have examined the volatile composition of rambutan seed oil. Thus, there is a need for a comprehensive characterization of the aroma profile of rambutan seed oil.Methods: This study systematically investigated and compared the aroma characteristics of three types (BR-4, BR-5 and BR-7) of rambutan seed oils using HS-SPME-GC-MS and HS-GC-IMS techniques, augmented by chemometric modeling.Results: GC-MS identified 135 volatile compounds, primarily hydrocarbons and esters, while GC-IMS characterized 35 compounds, mainly aldehydes and alcohols. Multivariate statistical analyses revealed significant differences in the aroma profiles among the oil samples. BR-5 exhibited the highest levels of aldehydes and hydrocarbons, whereas BR-7 demonstrated the highest content of esters and ketones. 21 and 15 differentially volatile compounds were extracted from the datasets obtained via GC-MS and GC-IMS. These compounds primarily comprised esters (floral and fruity) and aldehydes (green and fatty), interacting to create the distinctive aroma profiles of the three rambutan seed oils.Conclusion: This study provides theoretical support for evaluating the flavor characteristics and utilization potential of rambutan seed oil.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2019
·Vol. 20
·期 19
·4803
·
IF 4.183
Shan Jin,Kuan Shi,Liu Liu,Yu Chen,Guangzhong Yang
Xanthones are important chemical constituents ofGarcinia xanthochymusand varied bioactivities including cytotoxicity. However, their anti-tumor mechanism has remained unknown. Here, we isolated and identified a new xanthone named garciniaxanthone I (1) and five known compounds from the bark ofG. xanthochymus. Their structures were elucidated by NMR analysis and HRESIMS. The anti-proliferation activities of all isolated compounds were evaluated on four human tumor cell lines (HepG2, A549, SGC7901, MCF-7). The results demonstrated that the anti-proliferation activity of xanthone was related to the number and location of prenyl groups. We further found that garciniaxanthone I (GXI) could induce HepG2 apoptosis and enhance the expression of cleaved caspase-8, caspase-9, and caspase-3. GXI could also increase Bax level and concurrently reduce the overexpression of Bcl-2, Bcl-XL, Mcl-1, and surviving in HepG2 cells. Moreover, GXI could inhibit cell migration of HepG2 cells by inhibiting the expressions of MMP-7 and MMP-9. In summary, our study suggests that GXI could induce HepG2 apoptosis via the mitochondrial pathway and might become a lead compound for liver cancer treatment.Keywords:xanthones;Garcinia xanthochymus;apoptosis;HepG2;caspase;Bcl-2 family;MMPs
研究领域:
药理学
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肿瘤学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
PLoS One
·
2016
·Vol. 11
·期 9
·e0161566
·
IF 3.057
Ming Guo,Xiaomeng Wang,Xiaowang Lu,Hongzheng Wang,Peter E. Brodelius
In order to obtain the biological active compound, α-mangostin, from the traditional native mangosteen ( Garcinia mangostana L.), an extraction method for industrial application was explored. A high yield of α-mangostin (5.2%) was obtained by extraction from dried mangosteen pericarps with subsequent purification on macroporous resin HPD-400. The chemical structure of α-mangostin was verified mass spectrometry (MS), nuclear magnetic resonance ( 1 H NMR and 13 C NMR), infrared spectroscopy (IR) and UV-Vis spectroscopy. The purity of the obtained α-mangostin was 95.6% as determined by HPLC analysis. The binding of native α-mangostin to human serum albumin (HSA) or transferrin (TRF) was explored by combining spectral experiments with molecular modeling. The results showed that α-mangostin binds to HSA or TRF as static complexes but the binding affinities were different in different systems. The binding constants and thermodynamic parameters were measured by fluorescence spectroscopy and absorbance spectra. The association constant of HSA or TRF binding to α-mangostin is 6.4832×10 5 L/mol and 1.4652×10 5 L/mol at 298 K and 7.8619×10 5 L/mol and 1.1582×10 5 L/mol at 310 K, respectively. The binding distance, the energy transfer efficiency between α-mangostin and HSA or TRF were also obtained by virtue of the Förster theory of non-radiation energy transfer. The effect of α-mangostin on the HSA or TRF conformation was analyzed by synchronous spectrometry and fluorescence polarization studies. Molecular docking results reveal that the main interaction between α-mangostin and HSA is hydrophobic interactions, while the main interaction between α-mangostin and TRF is hydrogen bonding and Van der Waals forces. These results are consistent with spectral results.
研究领域:
药用植物学
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药物化学
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生物化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）