JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Yu Lu,Wanjun Jin,Yuerong Yang,Yue Jia,Lujia Sun,Jie Liu,Langhong Wang,Fuxing Zhang,Wupeng Ge,Jiansheng Wang,Linjuan Huang,Zhongfu Wang
Goat milk oligosaccharides are complex carbohydrates with a variety of biological functions. Free oligosaccharides from goat milk show more similarity to human milk than cow milk. At present, changes in goat milk glycoconjugates at different parities remain poorly studied. Herein, we qualitatively and quantitatively compared the goat milk glycoprotein N/O-glycome at different parities using a stable isotope labeling followed by electrospray ionization mass spectrometry and online hydrophilic interaction chromatography. N-Glycans were mainly fucosylated and nonfucosylated nonsialylated, and both fucosylation and sialylation gradually increased with parity, amounting (at the third parity) to 1.25 times and 3.3 times those of the first parity, respectively. O-Glycans were mostly nonfucosylated and nonsialylated, and sialylation increased with increasing parity, and Neu5Ac-sialylated was up to 9 times higher in the third parity than in the first parity, whereas Neu5Gc-sialylated was 5.5 times higher. This study provides a reference for exploring an alternative milk source closest to human milk and for the development of humanized formula milk.
研究领域:
食品科学
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生物化学
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营养学
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DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2023
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IF 4.3
Jing-Yan Li,Qian Ge,Bin Liu,Zhen-Peng Gao,Chun-Feng Guo
This study aimed to develop a non-protein-based method for quantify cow milk in adulterated goat milk. The raw cow milk and raw goat milk samples were mixed separately with the same volume of cold acetone and centrifuged to remove their proteins. The supernatants were evaporated, and the residues were derivatized by silylation followed by GC-MS analysis. N-acetylglucosamine was identified as a thermostable non-protein biomarker of cow milk. Its concentration in the raw cow milk (146.7 mg/L) was 32 times higher than that in the raw goat milk. On this basis, a HPLC-MS/MS method using positive-ion electrospray ionization in parallel reaction monitoring mode was developed for quantifying cow milk in adulterated goat milk by determining peak area of the biomarker. The method showed good linearity over cow milk concentrations of 1–100% and could detect as low as 0.3% cow milk in adulterated goat milk. Besides, the method was characterized by a simple sample pretreatment process, rapid detection, high accuracy, and high precision. With this method, a sample could be analyzed in 10 min. The present study demonstrated the feasibility of quantifying cow milk in adulterated goat milk using N-acetylglucosamine as a biomarker of cow milk.
研究领域:
食品科学
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分析化学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Zhi Cao,Zhengshi Chang,Yumei Gao,Bingjie Xue,Yanhui Yang,Lili Zhao,Wupeng Ge
This study focused on the effects of various non-thermal treatments on the antigenicity of goat milk whey protein (GWP), and the mechanism of reducing antigenicity was also discussed from the perspective of conformation epitope and linear epitope. The optimal reduction rate of antigenicity was 38.83% for β-Lg and 29.27% for α-La after 600MPa/30 min of ultra-high pressure (UHP) treatment. Non-thermal treatments not only changed the structure of GWP, but also increased or decreased the number, exposure degree and antigen binding rate of antigenicity peptides. Three kinds of non-thermal treatments reduced the GWP antigenicity by different mechanisms. UHP and PEF treatments reduced antigenicity mainly through altering conformational epitopes, while CP treatment reduced antigenicity by both altering conformational epitopes and decreasing antigenicity peptides. This result provided new ideas and technologies to reduce milk protein antigenicity.
研究领域:
食品科学
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免疫学
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蛋白质组学
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产品关联
DOI
高影响因子
Food Chemistry-X
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2025
·
IF 8.2
Longyu Wan,Wen Tu,Jiaxin Zhang,Jiayue Yang,Xu Wang,Jian He,Chaoxin Man,Qianyu Zhao,Feng Zhao,Yujun Jiang
Goat's milk-based infant formula (YIF) has the advantages of high nutritional value and hypoallergenicity, and fewer studies have been conducted on YIF. Therefore, this study examined the changes in physicochemical and functional properties of cow's milk-based infant formula (ZIF) and YIF during 6 months of storage at 25 °C, 37 °C, and 50 °C, respectively. The results showed that YIF had higher pH (7.26), wettability, protein oxidation, good wettability, and lower lactose crystallinity and lipid oxidation compared to ZIF. Further analysis revealed that the higher pH significantly accelerated the rate of the Maillard Reaction (MR), resulting in significantly higher browning of YIF than ZIF, and this difference was statistically significant ( p < 0.05). This is one of the reasons for the decrease in solubility of YIF. This paper explores the differences in storage stability of ZIF and YIF and provides theoretical guidance for the selection of milk-based ingredients.
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL DAIRY JOURNAL
·
2021
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IF 3.032
Ying Ma,Yanmei Hou,Kui Xie,Lina Zhang,Peng Zhou
Immunoglobulin G (IgG) and lactoferrin (Lf) play an important role in the protection and maturation of the neonatal immune system; however, the survival of IgG and Lf during gastrointestinal digestion and their digestive differences among human, bovine, and caprine milk remain unclear. This study investigated the digestive behaviour of IgG and Lf by simulating infant digestion of the milk of three species. Both IgG and Lf in human milk showed higher resistance to digestion than those in the other two types of milk. In addition, caprine milk IgG survived for a longer period after gastrointestinal digestion than bovine milk IgG. These static in vitro digestion results showed that IgG and Lf from human and caprine milk are digested more slowly from those from bovine milk.
研究领域:
营养学
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免疫学
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食品科学
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产品关联
DOI
高影响因子
FASEB JOURNAL
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2020
·Vol. 34
·期 6
·8671-8685
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IF 4.966
Jie Cai,Diming Wang,Shulin Liang,Jinrong Peng,Fengqi Zhao,Jianxin Liu
During lactation, an improper glucose supply often threatens mammary gland (MG) health. However, information is limited on the metabolic trajectories and molecules that regulate lactating MGs with an excessive glucose supply. Based on the network analysis of transcriptome and microRNAs, we found that the oversupply of glucose-induced severe glucose metabolic disorders in MGs of lactating goats, shifting lactose synthesis to acute fermentative glycolysis which caused increased flux of glucose metabolism into lactate. Moreover, NF-κB2 played a key role in regulating glycolysis, exhibiting a metabolic shift when MGs had an excessive supply of glucose. In primary mammary epithelial cells, fermentative glycolysis, and intracellular concentration of reactive oxygen species (ROS) were reduced by ganoderic acid A through blocking NF-κB2, while activation of NF-κB2 with phorbol myristate acetate (PMA) upregulated fermentative glycolysis and increased cellular ROS accumulation under excessive glucose. Thus, we established an NF-κB2-targeting method to reform the metabolic shift toward glycolysis caused by glucose oversupply by integrating NF-κB2 blockade and intracellular ROS scavenging.
研究领域:
动物生理学
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分子生物学
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营养学
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DOI
PubMed
高影响因子
Frontiers in Veterinary Science
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2025
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IF 2.9
Jie Luo,Yiping Wang,Wenya Zheng,Chaolong Xu,Jianhua Chen,Ben Liu,Xiaoyue Wang,Manxin Fang
Introduction Mastitis outbreaks in dairy goats at a farm in western Jiangxi Province, have recently imposed significant economic losses on farmers. This study aimed to identify the causative pathogen and implement targeted control measures for early disease eradication. Methods Pathological autopsy was performed on diseased goats submitted for examination. Pathological mammary gland tissues and milk cistern fluid were collected aseptically for bacterial isolation and purification. The isolated pathogen was characterized through colony morphology observation, Gram staining, biochemical tests, and 16S rDNA gene sequencing. Pathogenicity was assessed using an animal challenge test in mice. In vitro antibacterial susceptibility testing was conducted using 30 Antibiotics (e.g., cefradine, cephalexin, cefuroxime), 18 Chinese herbal medicines (CHMs; e.g., Rhus chinensis (Galla Chinensis), Coptis chinensis (Chinese Goldthread), Punica granatum (Granati Pericarpium)), and 15 phytogenic extracts (e.g., Berberine, Gallic acid, Tannic acid). Results A Gram-positive bacterium was isolated from the diseased tissues. Biochemical profiling and 16S rDNA sequencing identified the isolate as Staphylococcus caprae . The animal challenge test confirmed the isolate’s strong pathogenicity in mice. Susceptibility testing indicated that the isolate was susceptible to Antibiotics such as cefradine, cephalexin, cefuroxime, and ciprofloxacin, while showing the poorest susceptibility to penicillin among the tested antibiotics. Among the CHMs and phytogenic extracts, Rhus chinensis (Galla Chinensis), Coptis chinensis (Chinese Goldthread), Punica granatum (Granati Pericarpium), Berberine, Gallic acid and Tannic acid demonstrated superior antibacterial efficacy. Discussion This study provides multiple therapeutic and preventive options (CHMs, Antibiotics, phytogenic extracts) for mastitis control on this farm. It also offers valuable insights into the identification and treatment of mastitis in dairy goats, contributing to safeguarding goat health and ensuring dairy product safety.
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DOI
PubMed
高影响因子
Food Bioscience
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2024
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IF 4.8
Yusi Qin,Ning Wang,Xinlu Yuan,Juanjuan Luo,Junnan Wan,Yiting Zhao,Cunfang Wang,Xiaoning Zhang
This work investigated the protective effects of high Fischer's ratio oligopeptides from goat ( Capra hircus ) whey protein (HFO) against CCl 4 -induced acute liver injury in vivo and their underlying mechanism of action. The results showed that HFO inhibited the rise of serum transaminases (including alanine aminotransferase and aspartate aminotransferase), leading to an increase in the levels of superoxide dismutase and glutathione peroxidase and inhibition of malondialdehyde content, thus ameliorating the CCl 4 -induced liver lesions. Moreover, the levels of cytokines, tumor necrosis factor-α, interleukin-6, and interleukin-1β decreased significantly, indicating that HFO alleviates the CCl 4 -induced inflammatory response by reducing the levels of inflammatory factors in mice. Additionally, Western blot analysis showed that HFO treatment significantly promoted the expression of IkappaB-alpha antibody and inhibited the expression of phosphorylated p65, suggesting that the anti-inflammatory activity is one of the reasons for the hepatoprotective effects of HFO. In conclusion, HFO can potentially be used as a component of functional foods with a particular focus on treating chemical liver injury.
研究领域:
药理学
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毒理学
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免疫学
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生物化学
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产品关联
DOI
高影响因子
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