Current Research in Food Science
·
2024
·
IF 6.2
Jinjiao Dong,Wenjun Wang,Guodong Zheng,Nansheng Wu,Jingjing Xie,Shiyi Xiong,Panting Tian,Jingen Li
Choerospondias axillaris fruit has attracted more and more attention due to its various pharmacological activities, which are rich in polysaccharides. This study investigated the in vitro saliva-gastrointestinal digestion and fecal fermentation behaviors of polysaccharides from Choerospondias axillaris fruit (CAP), as well as its impact on human gut microbiota. The results showed that CAP could be partially degraded during the gastrointestinal digestion. The FT-IR spectra of the digested CAP didn't change significantly, however, the morphological feature of SEM changed to disordered flocculent and rod-like structures. 16S rRNA sequencing analysis found that after in vitro fermentation, CAP could increase the relative abundances of beneficial bacteria including Megasphaera , Megamonas and Bifidobacterium to produce short-chain fatty acids (SCFAs), while it can also reduce the abundances of harmful bacteria of Collinsella , Gemmiger , Klebsiella and Citrobacter , suggesting that CAP could modulate the composition and abundance of gut microbiota. These results implied that CAP can be developed as a potential prebiotic in the future.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2016
·
IF 3.994
Da-li Meng,Lei Shang,Xiao-he Feng,Xing-fei Huang,Xin Che
In order to increase the solubility of poorly water-soluble natural product, xanthoceraside, an effective anti-AD compound from Xanthoceras sorbifolia Bunge , and maintain its natural property, the xanthoceraside hollow gold nanoparticles were successively prepared by green ultrasonic method with silica spheres as templates and HF solution as selective etching solvent. Hollow gold nanoparticles and drug-loaded hollow gold nanoparticles were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetry (DSC). The solubilities of xanthoceraside loaded on hollow gold nanoparticles were increased obviously from 3.0 μg/ml and 2.5 μg/ml to 12.7 μg/ml and 10.7 μg/ml at 25 °C and 37 °C, respectively. The results of XRD and DSC indicated that the reason for this increase was mainly due to the amorphous state of xanthoceraside loaded on the hollow gold nanoparticles. In summary, the method of loading xanthoceraside onto hollow gold nanoparticles was a green and useful strategy to improve the solubility and dissolution of poorly water-soluble natural products and worth to applying to other natural products.
研究领域:
药剂学
·
纳米医学
·
绿色制药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2017
·
IF 2.313
Yangqing He,Xing Li,Ju Wang,Qian Yang,Binghua Yao,Yingjuan Zhao,Aiming Zhao,Wenxing Sun,Qian Zhang
Cornus officinalis has been widely used as a precious herb and as the tonic food to improve kidney function in China. Its fruits have been used in many traditional Chinese medicine prescriptions to treat kidney diseases, diabetes, cancer and shock. In this study, a new eco-friendly approach for green synthesis of silver nanoparticles (AgNPs) by using the fruits of Cornus officinalis aqueous extract as a reducing and stabilizing agent. The so-synthesized AgNPs showed quasi-spherical in shape with uniform dispersal and an average mean size of 11.7 nm. Water soluble biomolecules such as flavonoids and/or anthocyanins from the extract played important roles in the nanoparticles formation. The AgNPs displayed distinctive cytotoxicity activities against human prostate cancer (PC-3) and human liver cancer (HepG2) cell lines. The results provided a low cost, nontoxic and eco-friendly approach for synthesizing metal nanoparticles to explore alternative anticancer agents on the way fighting against cancer in future.
研究领域:
纳米技术
·
药理学
·
肿瘤学
·
植物学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2022
·
IF 6.212
Xin Liu,Hao Cai,Minjie Niu,Hui Yu,Mengqing Wang,Hui Zhu,Gang Cao,Ke Pei,Yu Duan,Jiantao Song
Fructus Corni (FC), as a promising Chinese medicinal herb, has aroused considerable interest. Generally, FC needs to be processed according to the limited standard policy in China before clinical application, while the investigations on the specific processing methods (such as wine steaming or high-pressure wine steaming) are unclear. A comprehensive metabolomics strategy based on integrated non-targeted metabolomics and targeted glycomics in this paper was implemented to investigate the influences of the different processing technologies such as steaming, wine steaming, high-pressure steaming, high-pressure wine steaming, wine immersion, and wine stir-frying on FC, respectively. UHPLC-Q-TOF-MS/MS was employed for identifying and distinguishing the secondary metabolites. A total of 85 components were identified in all groups. The results of PCA score plots showed that the crude and processed samples had a complete separation, and wine steamed and high-pressure wine steamed samples could be a category, indicating that the two processed products had a similar quality. Multiple chromatography including HPLC (C 18 )-PDA, HPLC (NH 2 )-ELSD, and HPGPC-ELSD was used for determining the molecular weight distributions, the monosaccharide compositions of polysaccharides, and the contents of free monosaccharides and oligosaccharides. The results indicated that the content and composition of saccharides were different in crude and different processed FC. The polysaccharides were composed of fucose, arabinose, galactose, glucose, galacturonic acid, mannose and rhamnose, and the free monosaccharides were composed of fucose, arabinose, galactose, glucose, mannose, rhamnose and fructose in all FC samples. The PCA score plots of the glycomics indicated that the crude and high-pressure wine steamed FC could be a category, showing that the two groups had similar chemical compositions. Ultimately, the simulation processing experiments indicated that the transformation of morroniside, polysaccharides, oligosaccharides, fructose, and glucose to 5-HMF through the reactions of dehydration and deglycosylation was the potential mechanism of enhancing the effects by processing. Conclusionly, the saccharides should be investigated as thoroughly as the secondary metabolites, and the high-pressure wine steamed FC could be an alternative to wine steamed FC.
研究领域:
药用植物学
·
代谢组学
·
食品科学
·
中医药
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Ningning Zhang,Lili Yang,Yongpeng Ma,Jinyong Huang,Shixin Zhu,Hao Dong,Zhuang Zhao,Yang Lu
Cornus officinalis Siebold & Zucc. is an important Chinese herbal medicine with traditional clinical applications, a long history of cultivation and high economic value in China. C. officinalis is distributed mainly in the Shaanxi, Henan, and Zhejiang provinces of China, which are also historically the main production areas, and which still provide 90% of the medicinal material of this species for the contemporary market. In this study, we investigated the main germplasm resources of C. officinalis across its distribution, based on fruit morphology, the concentrations of selected medicinally active ingredients, and molecular population genetic analysis. The results indicated that the C. officinalis populations sampled had abundant variation in fruit morphology and chemical component content, and showed high genetic diversity , as revealed by microsatellite markers. Clustering results based on morphology, active ingredient concentration and microsatellite markers supported the idea that populations from Zhejiang province were distinct from those in Shaanxi and Henan. The fruit and molecular data suggested that the Shaanxi and Henan populations were mixed, which could be attributed to their close geographical distance and frequent germplasm exchange . Most of the C. officinalis populations had relatively weak genetic differentiation from others sampled, and most of the individuals sampled showed extensive admixture. We suggest that artificial gene flow caused by intensive cultivation and widespread trading is responsible for the observed admixture of genetic components and blurred genetic boundaries between C. officinalis populations, especially in the Shaanxi and Henan populations. We also propose some suggestions for the efficient utilization and conservation of C. officinalis germplasm. These findings provide valuable information on the genetic resources of C. officinalis and offer guidelines for breeding programs and scientific management of C. officinalis .
研究领域:
植物学
·
遗传学
·
药理学
·
植物育种
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2017
·
IF 3.981
Wenhua Ji,Tao Wang,Wei Liu,Feng Liu,Lanping Guo,Yanling Geng,Xiao Wang
An efficient, accurate and sensitive method for the determination of five iridoid glycosides (IGs) in Cornus officinalis fructus using molecularly imprinted solid phase extraction (MISPE) coupled with high performance liquid chromatography (HPLC) has been developed. Water-compatible molecularly imprinted beads (MIBs) were synthesized by precipitation polymerization, using alkenyl glycosides glucose as the hydrophilic functional monomer. Scanning electron microscopy showed that the MIBs had a narrow particle size distribution, with diameters in the range 7.5–9.3 μm. The special molecular recognition by the MIBs of IGs in aqueous media was verified by static adsorption, kinetic adsorption, and selectivity experiments. The newly prepared MIBs were used as sorbents in solid phase extraction (SPE) for the selective recognition of five IGs (loganin, morroniside, loganic acid, 7- O -ethyl-morroniside and 7- O -methyl morroniside) in Cornus officinalis fructus. When optimized, the MISPE-HPLC method had good linearity (0.02–100 mg g −1 ), with correlation coefficient ( R ) ≥ 0.994. Recoveries at three spiked levels were in the range 80.0%–94.0%. Because of its excellent specificity and hydrophilicity, SPE based on monodisperse MIBs provides a promising pretreatment strategy for the analysis of active components in natural products, especially for the quality control of traditional Chinese medicines.
研究领域:
分析化学
·
药学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2020
·Vol. 15
·期 5
·e0227844
·
IF 2.74
Renjie Xu,Hongdan Zhu,Lingmin Hu,Beimeng Yu,Xiaohua Zhan,Yichu Yuan,Ping Zhou
Morroniside is a biologically active polyphenol found in Cornus officinalis Sieb . et Zucc (CO) that exhibits a broad spectrum of pharmacological activities, such as protecting nerves, and preventing diabetic liver damage and renal damage. However, little data are available regarding the mechanism of its intestinal absorption. Here, an in vitro human intestinal epithelial cell model of cultured Caco-2 cells was applied to study the absorption and transport of morroniside. The effects of donor concentration, pH and inhibitors were investigated. The bidirectional permeability of morroniside from the apical (AP) to the basolateral (BL) side and in the reverse direction was studied. When administered at three tested concentrations (5, 25 and 100 μM), the apparent permeability coefficient (P app ) values in the AP-to-BL direction ranged from 1.59 × 10 −6 to 2.66 × 10 −6 cm/s. In the reverse direction, BL-to-AP, the value was ranged from 2.67 × 10 −6 to 4.10 × 10 −6 cm/s. The data indicated that morroniside transport was pH-dependent. The permeability of morroniside was affected by treatment with various inhibitors, such as multidrug resistance protein inhibitors MK571 and indomethacin, as well as the breast cancer resistance protein inhibitor apigenin. The mechanisms of the intestinal absorption of morroniside may involve multiple transport pathways, such as the passive diffusion and efflux protein-mediated active transport especially involving multidrug resistance protein 2 and breast cancer resistance protein. After the addition of CO, the P app values in the AP-to-BL direction increased significantly, therefore, it can be assumed that some ingredients in the CO promote morroniside absorption in the small intestine.
研究领域:
药理学
·
肠道吸收机制
·
天然产物研究
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OncoTargets and Therapy
·
2021
·
IF 4.147
Ruiqi Xu,Mengnan Zeng,Yuanyuan Wu,Shengchao Wang,Beibei Zhang,Jingke Zhang,Yuxuan Kan,Benke Li,Bing Cao,Xiaoke Zheng,Weisheng Feng
Purpose This research aims to investigate the intervention and mechanism of 50% acetone extract of C. officinalis leaves (SZYY) on melanoma xenografts. Patients and Methods Tumor size and cardiac function were measured via ultrasound. The accumulation of 2-deoxy-D-glucose (2-DG) in tumor tissue was examined with near-infrared in vivo imaging. Flow cytometry was performed to assess apoptosis and reactive oxygen species (ROS) levels in tumor and immune cells in spleen. The levels of inflammatory cytokines in serum were detected by cytometric bead array. The expression of proliferation-, apoptosis-, and angiogenesis-related proteins in tumor cells was measured to evaluate the underlying mechanisms. Subsequently, the effects of four compounds separated from SZYY on the proliferation and migration of A375 cells and STAT3 signaling were examined. The peak identification and contents of the four components were performed via high-performance liquid chromatography (HPLC). Finally, we evaluated the inhibitory effects of STAT3 overexpression on the cytotoxic activity of four constituents in A375 cells. Results SZYY inhibited the growth and glycolysis of melanoma xenograft in mice, improved cardiac function, increased the percentages of macrophages, neutrophils, and lymphocytes in spleen, reduced the levels of IL-6, IL-17A, TNF-α, and IFN-γ in serum, promoted apoptosis and oxidative stress in tumor tissues, and inhibited STAT3 phosphorylation and expression of angiogenic factors. Chemical analysis showed that SZYY is rich in loganin, rutin, triohimas C, and triohimas D, which all could restrain the proliferation and migration of A375 cells and inhibit the phosphorylation and nuclear translocation of STAT3. Moreover, STAT3 overexpression could diminish the cytotoxic activity of four compounds on A375 cells. Conclusion SZYY could exert anti-melanoma effects via inhibiting STAT3 signaling to induce apoptosis and inhibit tumor angiogenesis. Its active ingredients might be loganin, rutin, triohimas C, and triohimas D.
研究领域:
肿瘤学
·
药理学
·
分子生物学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Qian Li,Xieyi Wang,Jun Chen,Chengmei Liu,Ti Li,David Julian McClements,Taotao Dai,Jiyan Liu
An extract isolated from Choerospondias axillaris peels was separated into five fractions using size-exclusion chromatography. The structural composition and mean degree of polymerization (mDP) of these fractions were then characterized by acid-catalysis followed by HPLC analysis. The antioxidant activity of each fraction was determined using a combination of chemical-based methods (DPPH , ABTS + radical scavenging activity, ferric-reducing antioxidant power, and phosphomolybdate assay) and a cellular-based assay. All fractions tested were found to have high total phenolics contents and were rich in proanthocyanidins. The mDP of fractions (F1–F5) ranged from 1.92 to 9.25. When tested by the chemical-based assays, the antioxidant activity of the fractions did not depend on molecular weight of the phenolics. Conversely, when tested by the cellular-based assay the antioxidant activity actually decreased with increasing molecular weight of the proanthocyanidins. These experiments highlight the limitations of using chemical-based assays to establish the antioxidant activity of proanthocyanidins within biological systems.
研究领域:
药理学
·
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2018
·
IF 3.52
Qian Li,Chengmei Liu,Ti Li,David Julian McClements,Yinxin Fu,Jiyan Liu
Epidemiological studies had indicated that the incidence of colorectal cancer is inversely correlated to the consumption of proanthocyanidins (PAs). In this study, five PAs fractions isolated from Choerospondias axillaris fruit peels with different mean degree of polymerization (mDP) were characterized and their antiproliferative effects on Caco-2 cells were investigated. MALDI-TOF-MS results indicated that PAs fractions were composed of procyanidins with DP up to 13-mers. All PAs fractions induced dose and time-dependent reductions of Caco-2 cell viability. The IC 50 values of PAs fractions with increasing mDP were 240 ± 16.0, 143 ± 9.53, 87.0 ± 3.55, 44.3 ± 4.89, and 42.8 ± 2.20 μg/mL after 24 h incubation, respectively. The observed reduction in Caco-2 cell viability was due to apoptosis via the activation of caspase-9, caspase-3, caspase-8 and the elevation of intracellular ROS generation. Moreover, the extent of the reduction in cell viability after exposure to PAs fractions was positively correlated with their mDP and galloylation. These results indicate that Choerospondias axillaris peel is a potential source of natural chemopreventive agents for the treatment of colorectal cancer.
研究领域:
药理学
·
肿瘤学
·
植物科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2024
·
IF 2.3
Huafeng Yang,Hui Liu,Yuan Zheng,Bo Li,Shujing Wang,Jun Zhang,Jiandong Wang
Cornus officinalis total glycosides (COTG) derived from the traditional Chinese medicine Cornus officinalis, is a natural immunosuppressant and has been extensively studied in immunomodulation and immunosuppression. This study aimed to explore the effects of COTG on granulomatous lobular mastitis (GLM) and its associated mechanisms. Compared to the model group, COTG effectively ameliorated histopathological damage to breast tissue, reduced mammary gland suppuration, and enhanced the blood-milk barrier. Additionally, COTG treatment reduced the total number of T cells and B cells in GLM rats, significantly improving clinical indicators such as P-selectin, E-selectin, and intercellular cell adhesion molecule-1. We also observed downregulation of CD28 and B7 expression levels, an upregulation of cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) expression, and a significant decrease in inflammatory marker levels in the COTG group. COTG exerts an anti-inflammatory effect in GLM by stimulating CTLA-4, inhibiting the B7-CD28 signaling pathway affecting T cell activation, and promoting the blood-milk barrier. These findings suggest that COTG could be a promising therapeutic option for managing GLM, potentially improving patient outcomes by modulating immune responses and reinforcing the blood-milk barrier.
研究领域:
药理学
·
免疫学
·
病理学
·
中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Luyao Xu,Xiujun Zhang,Wanyu Yang,Haixiang Li,Jiaqi Wang,Lan Wang,Xicheng He,Yan Wang,Xiaoxia Li
Plant phenolic compounds serve as significant natural antioxidants. However, traditional preparation methods face sustainability challenges. In this study, we synthesized a novel deep eutectic solvent (DES) modified core–shell nanomaterial composed of betaine, ethylene glycol and dopamine hydrochloride based on the spatial arrangement formed by the abundant weak interaction forces in the poly(deep eutectic solvent) (PDES). Utilizing the PDES magnetic nanomaterial and combined with ultrasound-assisted matrix solid phase dispersion (UA-MSPD), it was able to efficiently enrich Fructus Choerospondias phenols (FCP) up to 61.468 ± 0.405 mg/g. Density functional theory (DFT) was used to analyze the adsorption mechanism between the material and the target compounds, which involved weak interactions of electrostatics, hydrogen bonds, and π-π stacking, while maintaining the activity of phenols without destroying the molecular structure. Remarkably, the adsorption efficiency of the material remained above 80 % after six repetitions, indicating excellent reusability. FCPs were identified and analyzed using ultra-performance liquid chromatography-tandem triple quadrupole mass spectrometry (UPLC-Q-TOF-MS). The FCP prepared by Fe 3 O 4 @SiO 2 @PDES exhibited superior in vitro antioxidant performance, achieving enrichment and purification in one step. Cell experiment results showed that FCP could scavenge ROS, regulate cytokines, and reduce ferroptosis caused by ultraviolet radiation, making FCP have therapeutic value in practical applications. The technology opens up a new way for the green and sustainable production and enrichment of plant phenolic compounds for anti-aging treatment.
研究领域:
材料科学
·
药学
·
生物技术
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）