INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Caixuan Zhang,Ying Shu,Yang Li,Fanyu Wang,Juntian Gan,Yiqian Wang,Xiaojuan Feng,Mingzhu Guo
Clinical research has demonstrated that non-starch polysaccharides from natural sources exhibit protective and therapeutic effects on ulcerative colitis (UC). In this study, a non-starch polysaccharide (CYP-A) with a molecular weight of 1.54 × 10 3 kDa was isolated from a speciality Chinese yam ( Dioscorea ) variety, the Ma yam, consisting mainly of mannose and glucose. The results indicated that CYP-A alleviated colitis symptoms induced by dextran sulfate sodium (DSS), repaired the mucus barrier, and protected the integrity of the intestinal mechanical barrier. Furthermore, CYP-A suppressed pro-inflammatory cytokine production, reduced oxidative stress, and modulated the biological barriers by facilitating the colonization of norank_f-_Muribaculaceae , Dubosella , Faecalibaculum and Enterorhabdus, while reducing Escherichia - Shigella and Bacteroides . Notably, CYP-A reshaped the metabolic pathways related to steroid hormone biosynthesis as well as phenylalanine, tyrosine, and histidine metabolism. These findings highlight the potential of CYP-A as a nutraceutical for targeting UC and improving gut health.
研究领域:
营养学
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肠道微生物组学
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免疫学
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DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 9
·1772
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IF 5.2
Shihua Wu,Xianxiang Chen,Ruixin Cai,Xiaodie Chen,Jian Zhang,Jianhua Xie,Mingyue Shen
This study aimed to test the preventive anti-inflammatory properties of Chinese yam polysaccharides (CYP) and sulfated Chinese yam polysaccharides (SCYP) on LPS-induced systemic acute inflammation in mice and investigate their mechanisms of action. The results showed that SCYP can efficiently reduce plasma TNF-α and IL-6 levels, exhibiting an obvious anti-inflammation ability. Moreover, SCYP reduced hepatic TNF-α, IL-6, and IL-1β secretion more effectively than CYP, and significantly altered intestinal oxidative stress levels. In addition, a 16S rRNA gene sequencing analysis showed that CYP regulated the gut microbiota by decreasingDesulfovibrioandSutterellaand increasingPrevotella. SCYP changed the gut microbiota by decreasingDesulfovibrioand increasingCoprococcus, which reversed the microbiota dysbiosis caused by LPS. Linear discriminant analysis (LDA) effect size (LEfSe) revealed that treatment with CYP and SCYP can produce more biomarkers of the gut microbiome that can promote the proliferation of polysaccharide-degrading bacteria and facilitate the intestinal de-utilization of polysaccharides. These results suggest that SCYP can differentially regulate intestinal flora, and that they exhibit anti-inflammatory effects, thus providing a new reference to rationalize the exploitation of sulfated yam polysaccharides.Keywords:yam polysaccharide;sulfation;LPS;anti-inflammation;gut microbiota
研究领域:
免疫学
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微生物学
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药理学
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL SCIENCES
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2025
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IF 3.8
Yuehan Cui,Meng Song,Ruilai Liu,Zhenhao Xi,Ling Zhao,Lian Cen
A novel liposome nanoparticle loaded with yam polysaccharide was developed in this study via a tailor-made microfluidic device and further optimized through the Box-Behnken Design method of surface analysis to exploit combinational immunomodulatory effects of liposomes and yam polysaccharide. A chip of 100 μm in height and 200 μm in width with a mixing channel with embedded baffles to enhance fluid mixing was first designed and fabricated. Liposome nanoparticles were obtained by manipulating the operating parameters of the microfluidic chip. The formulation of yam polysaccharide loaded liposomes (YPL 1 ) was optimized using response-surface analysis, and their physicochemical properties were characterized. In vitro cellular assays were performed to assess the effect of YPL on the activity of mouse dendritic cells (DC) and the secretion of immune-related cytokines to investigate the immune-enhancing effect of YPL in vitro. The resulting YPL had a mean size of 154.2±3.8 nm with a narrow size distribution (PDI=0.083) and had a high entrapment efficiency (EE) of 79.02 %. The YPL exhibited good stability at 4 °C over 14 days of storage, and an in vitro sustained release duration of approximately 30 h. The YPL demonstrated excellent biocompatibility and stimulated the expression of immune-related cytokines, especially TNF-α. Therefore, the YPL with well controlled particle size and high loading capacity, capable of exhibiting potent immunostimulatory activity was successfully developed in this study via a microfluidic device and optimized using response-surface analysis. The current YPL engineering methodology could further serve as an experimental platform for liposome nanoparticle development.
研究领域:
药剂学
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免疫学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Yajuan Bai,Yue Zhou,Ruifen Zhang,Yanxia Chen,Fengzhong Wang,Mingwei Zhang
Plant polysaccharides possess many health-promoting properties, which are closely related to the digestion, absorption and utilization of polysaccharides in the host. The characteristic changes in Chinese yam polysaccharide (CYP) during simulated gastrointestinal digestion and faecal fermentation, as well as the intestinal anti-inflammatory effects of CYP and fermented CYP, were studied. The molecular weight of CYP remained unchanged during gastrointestinal digestion. After 24 h of faecal fermentation, free glucose and mannose released from CYP were utilized by gut microbiota, and the production of short-chain fatty acids increased. Simultaneously, CYP improved the growth of Bifidobacterium and Megasphaera. Compared with CYP, faecal fermented CYP exhibited greater suppression of anti-inflammatory mediators and increased intestinal tight junction expression in LPS-stimulated coculture Caco-2/Raw264.7 cells. The obtained results reveal that the role of CYP in promoting gut health is closely related to the interactions between CYP and gut microbes, and CYP has potential as an anti-inflammatory adjuvant in enteritis therapy.
研究领域:
营养科学
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微生物学
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免疫学
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药理学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Chao Zang,Yanju Zhang,Wenge Yang,Yonghong Hu
In this study, the electrospun nanofibrous membranes were prepared by blending electrospinning technology with Polycaprolactone/Chitosan (PCL/CS) as a spinning carrier and Chinese Yam Polysaccharide (CYP) as active material. The membranes were characterized and antimicrobial tested to determine whether they have potential value as food packaging. The results of SEM showed that the nanofiber membranes with different concentrations had good fiber morphology, the average fiber diameter was 313 ± 178 nm–426 ± 237 nm, and the PDI value indicated that the nanofiber was polydisperse. The addition of CYP enhanced the hydrophilicity and water solubility of the nanofiber membranes, but the mechanical strength of the membranes decreased. The spectra of FTIR and DSC indicated that the PCL/CS nanofiber membrane successfully loaded CYP, and the 4 % CYP nanofiber membrane had the best thermal stability. In vitro release tests also showed that most fibrous membranes still released CYP (56.4–81.7 %) after 240 h. In addition, the antibacterial zone diameter of PCL/CS/10 % CYP nanofiber membrane against Escherichia coli and Staphylococcus aureus was 22.01 ± 1.23 mm and 18.40 ± 0.84 mm, respectively, showing obvious antibacterial effect. In the tomato antibacterial experiment, PCL/CS/10 % CYP nanofiber membrane effectively delayed the water-loss rot of cherry tomato and kept the weight loss rate of fruit at 17.6 ± 0.14 %. The results showed that PCL/CS/10% CYP nanofiber membrane had the best effect as active food packaging.
研究领域:
材料科学
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生物技术
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食品科学
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高分子科学
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纳米技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）