Songsong Zhang,Beibei Qiu,Jinhua Zhu,Weiping Hu,Fanyi Ma,M. Z. H. Khan,Xiuhua Liu
Present study aimed to immobilise the α-glucosidase on suitable supports to construct enzymatic microreactors and their subsequent applicability in efficient inhibitor screening from the Chinese Yam (Dioscorea opposita Thunb.) peel. A type of lamellar and porous composites (rGO@Fe3O4) were synthesised with a facile one-step solvothermal method and employed as carriers to construct enzymatic microreactors for screening α-glucosidase ligand from the Chinese Yam peel in league with the high performance liquid chromatography and mass spectrometry (HPLC-MS). The immobilisation amount of α-glucosidase on rGO@Fe3O4 under the optimised conditions was about 40 μg α-glucosidase/mg carriers. Furthermore, the binding capacities of screened inhibitors, 2,4-dimethoxy-6,7-dihydroxyphenanthrene and batatasin I, were 35.6 and 68.2%, respectively. Hence, considering their high screening efficiency and excellent magnetic separation ability, these as-prepared nanocomposite consisting of rGO and Fe3O4 may be potential supports for the enzyme (such as α-glucosidase) immobilisation for rapid α-glucosidase inhibitors screening from the diverse nature resources.
Yan Wang,Rongyao Chen,Zijing Dai,Qingcai Yu,Yongmei Miao,Ronghua Xu
In this study, Chinese yam peel (CYP) was modified with polypyrrole via an in situ polymerization method to remove Congo red from aqueous media. The prepared CYP–polypyrrole (CYP–PPy) composite was characterized using FTIR, SEM, TEM, XRD, TG and BET analysis. The performance of CYP–PPy towards the adsorption of Congo red (CR) was explored in batch mode. The removal efficiency of CR was found to be 86% at the initial concentration of 100 mg L−1, contact time of 120 min, and the adsorbent dosage of 10 g L−1. At equilibrium time (20 h), the removal efficiency was significantly acceptable (98.9%). The adsorption kinetics data were most consistent with the pseudo-second-order kinetic model. The adsorption equilibrium data could be described well by the Langmuir isotherm model with the maximum adsorption capacity of 86.66 mg g−1. In view of thermodynamics, the adsorption process was endothermic and more favorable for CR removal at 45 °C. A reusability study indicated that CYP–PPy could be reused effectively for up to three successive cycles of ad-/de-sorption. Hence, this work provides an alternative scheme for the targeted exploitation of agricultural waste to control dye pollution.
危险品化学品经营许可证（带存储）