Biotechnology Journal
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2024
·Vol. 19
·期 11
·e202400489
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IF 3.2
Bo Zhang,Sifang Zhu,Yi Zhu,Xin Sui,Junping Zhou,Zhiqiang Liu,Yuguo Zheng
Boldenone (BD), a protein anabolic hormone, is commonly used to treat muscle damage, osteoporosis, and off-season muscle building in athletes. Traditional BD synthesis methods rely on chemical processes, which are costly and environmentally impactful. Therefore, developing a more sustainable and economical biosynthetic pathway is crucial for BD production. This study aimed to achieve efficient production of BD. Firstly, the catalytic performance of 17β-hydroxysteroid dehydrogenase and 3-ketosteroid-Δ 1 -dehydrogenase was improved through enzyme engineering, and their expression in the new strain of Mycobacterium neoaurum was enhanced using metabolic engineering. These improvements significantly increased BD production to 4.05 g/L, with a significant decrease in by-product generation. To further increase the yield, a multi-enzyme fusion expression system was constructed, and a key cell wall gene kasB was knocked out, resulting in a spatial-time yield of BD reaching 1.02 g/(L·d). Subsequent optimization of the transformation system further increased the BD production to 5.56 g/L, with a spatiotemporal yield of 1.39 g/(L·d). The green biosynthetic route of phytosterol one-step conversion to BD developed in this study lays the foundation for industrial production.
研究领域:
生物化学
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微生物学
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合成生物学
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产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Shijun Xu,Yi He,Siming Yan,Teng He,Hongjie Li,Yuan Gao,Wen Chen,Jin Yan,Guiyang Wu,Xi Yuan
This article elucidates the synthesis of NiMoO 4 -based catalysts with different arrays and crystal structures, including nanowires (nw), nanoplates (ns), and nanocolumns (nc). Subsequently, a high-temperature phosphorization method was employed to generate the corresponding P-NiMoO 4 catalysts. The study investigates the structural evolution of the catalysts and changes in the active species under operational conditions. Concurrently, density functional theory (DFT) calculations provide a more in-depth description of the HER reaction mechanism, considering d-band centers and adsorption energies. Experimental results demonstrate that phosphorized NiMoO 4 with a nanowire array structure and crystal structures of NiMoO 4 and MoO 3 exhibit the optimal HER performance, displaying superhydrophilic and underwater superaerophobic behaviors. The hydrophilic contact angle is 0°, and the aerophobic contact angle is 156.8° This array electrode achieves a current density of 10 mA·cm −2 at an overpotential as low as 73.97 mV. The experiments indicate that through doping and morphology control engineering, catalytic performance can be further synergistically enhanced, providing a new perspective for preparing HER catalytic electrodes with potential applications.
研究领域:
材料科学
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催化化学
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理论计算
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产品关联
DOI
高影响因子
CRYSTENGCOMM
·
2019
·Vol. 22
·期 2
·248-256
·
IF 3.382
Xiuxia Wang,Chengyan Liu,Junliang Chen,Lei Miao,Shaohai Wu,Xiaoyang Wang,Zhengchuan Xie,Wenjing Xu,Qiufeng Chen
Recently, Ag8SnSe6-based materials, belonging to the family of argyrodite compounds, have been considered as promising thermoelectric candidates due to their intrinsically ultralow lattice thermal conductivity. However, their thermoelectric properties have been rarely investigated because of the intrinsic low carrier concentration. In this work, polycrystalline Ag8SnSe6 bulk materials were successfully synthesized by a bottom-up method by using hydrothermally synthesized Ag8SnSe6 nanocrystals as the precursors. The carrier concentration and mobility of Ag8SnSe6 are successfully enhanced by introducing additional Sn, which should manipulate the self-defects and improve the crystallinity. Thanks to the synergistic optimization of crystal and composition engineering, a maximum ZT value of about 1.22 is achieved for Ag8Sn1.005Se6 at 715 K, which is about 11% higher than that of the pristine sample, and the average ZT is also improved from about 0.65 to about 0.87. This study indicates that crystal engineering and composition engineering play an important role in the thermoelectric properties of Ag8SnSe6 and may provide guidance to improve the thermoelectric performance of other argyrodite compounds.
研究领域:
材料科学
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热电材料
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Yufei Zhang,Junhua Yun,Guoyan Zhang,Amreesh Parvez,Lei Zhou,Hossain M. Zabed,Jia Li,Xianghui Qi
3-Hydroxypropionic acid (3-HP) is a top value-added chemical with multifaceted application in chemical, material, and food field. However, limited availability of robust strains and elevated fermentation costs currently impose constraints on sustainable biosynthesis of 3-HP. Herein, transporter engineering, metabolic dynamic modulation, and enzyme engineering were combined to address above limitations. First, a glucose-utilizing 3-HP biosynthetic pathway was constructed in Escherichia coli , followed by recruiting alternative glucose transport system to overcome center metabolism overflow. Next, the Cra (a transcription factor)-dependent switch was applied to autonomously fine-tune carbon flux, which alleviated growth retardation and improved the 3-HP production. Subsequently, inactivation of glycerol facilitator (GlpF) increased intracellular glycerol levels and boosted 3-HP biosynthesis, but caused toxic intermediate 3-hydroxypropionaldehyde (3-HPA) accumulation. Furthermore, semi-rational design of aldehyde dehydrogenase (YdcW) increased its activity and eliminated 3-HPA accumulation. Finally, fed-batch fermentation of the final strain resulted in 52.73 g/L 3-HP, with a yield of 0.59 mol/mol glucose.
研究领域:
生物化学
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合成生物学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2021
·
IF 5.316
Jinzhu Wu,Yue Liu,Yuan Yao,Yanbin Shao,Xiaohong Wu
Superabsorbers can find application in the fields of solar cells, blackbody radiation, thermal control systems, and stray light elimination devices. Herein, graphene-like 2H/1T-molybdenum diselenide (MoSe 2 ) was synthesized via a hydrothermal method and shows superhigh solar absorption (~99%) within a range of 200–2500 nm. In the synthesis, selenium dioxide (SeO 2 ) was used as a Se source instead of Se powder, which plays an important role in manipulating the graphene-like morphology and the multiphasic composition of the MoSe 2 . Consequently, the as-prepared MoSe 2 nanoflowers possess desired band structure and abundant pores and pore channels for strong optical absorption. DFT calculations clarify that the 1T-MoSe 2 primarily contributes to the superior optical absorption over the 2H-MoSe 2 . This work provides a facile strategy for enhancing the optical absorption of two-dimensional transition metal dichalcogenides (2D TMDCs) by engineering morphology and phase.
研究领域:
材料科学
·
物理化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Guiwei Yan,Liqin Xu,Bijun Fang,Shuai Zhang,Xiaolong Lu,Xiangyong Zhao,Jianning Ding
A novel dual priority strategy of strengthening charge compensation in A-site of perovskite structure and widening bandgap width was designed to prepare (Ba 0.98- x Li 0.02 La x )(Mg 0.04 Ti 0.96 )O 3 (BLLMT x ) ceramics, which can solve the conflict between polarization and breakdown strength, and improve the pulse energy storage performance of the BaTiO 3 -based system. As a result, ultrahigh discharge energy density of 3.98 J/cm 3 and giant pulse power density of 321.6 MW/cm 3 with current density of 2786.4 A/cm 2 are obtained at 360 kV/cm for the (Ba 0.94 Li 0.02 La 0.04 )(Mg 0.04 Ti 0.96 )O 3 (BLLMT 0.04 ) ceramics with considerable fast discharge rate and outstanding temperature stability as well as cycle life, correlating with multi-ferroelectric phases coexistence, increasing bandgap width, strengthening charge compensation and establishing small size of the polar nano-regions (PNRs), which exceeds the great mass of reported lead-free ceramics. The comprehensive properties indicate that the BLLMT 0.04 ceramics present potential application in pulse energy storage system. The concept of composition design via increasing bandgap width and strengthening charge compensation provides a new idea for developing lead-free dielectric ceramic capacitors.
研究领域:
材料科学
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能源存储
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产品关联
DOI
高影响因子
Fermentation-Basel
·
2023
·Vol. 9
·期 10
·906
·
IF 3.7
Yilu Wu,Changsheng Su,Gege Zhang,Zicheng Liao,Jieyi Wen,Yankun Wang,Yongjie Jiang,Changwei Zhang,Di Cai
Bioethanol is an important biofuel which can be produced from the abundant low-value lignocelluloses. However, the highly toxic inhibitory compounds formed in the hydrolysate and the ineffective utilization of xylose as a co-substrate are the primarily bottlenecks that hinder the commercialization of lignocellulosic bioethanol. In this study, aiming to properly solve the above obstacles, an engineeredSaccharomyces cerevisiaestrain was constructed by introducing the xylose reductase (XR)–xylitol dehydrogenase (XDH) pathway, overexpressing the non-oxidized pentose phosphate pathway, and deleting aldose reductaseGRE3and alkaline phosphatasePHO13using a GTR-CRISPR system, followed by adaptive laboratory evolution (ALE). After screening, the isolatedS. cerevisiaeYL13-2 mutant was capable of robust xylose-utilizing, and exhibited high tolerance to the inhibitors in undetoxified steam-exploded corn stover hydrolysate (SECSH). An ethanol concentration of 22.96 g/L with a yield of 0.454 g/g can be obtained at the end of batch fermentation when using SECSH as substrate without nutrient supplementation. Moreover, aiming to simplify the downstream process and reduce the energy required in bioethanol production, fermentation using fed-batch hydrolyzed SECSH containing higher titer sugars with a YL13-2 strain was also investigated. As expect, a higher concentration of ethanol (51.12 g/L) was received, with an average productivity and yield of 0.71 g/L h and 0.436 g/g, respectively. The findings of this research provide an effective method for the production of bioethanol from lignocellulose, and could be used in large-scale applications in future works.Keywords:Saccharomyces cerevisiae;bioethanol;steam explosion;metabolic engineering;evolution engineering
研究领域:
生物技术
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生物化学工程
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微生物学
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产品关联
DOI
高影响因子
ACS Synthetic Biology
·
2025
·
IF 3.9
Yifan Feng,Wenlong Xiao,Xinyi Li,Weiyu Cao,Yujia Jiang,Wenming Zhang,Wankui Jiang,Fengxue Xin,Min Jiang
Ectoine, a major compatible solute in halophilic micro-organisms, shows great potential in cosmetics and pharmaceuticals areas owing to its water-binding properties and capability to prevent oxidative damage. In this study, the ectABC gene cluster responsible for the ectoine synthesis originated from halophilic bacterium Halomonas venusta was first assembled into Escherichia coli. Subsequently, the crr gene in PTS was knocked out to further drive the metabolic flux from phosphoenolpyruvate to oxaloacetate, resulting in 1.27 g/L of ectoine. Then, the rate-limiting enzyme LysC in the ectoine synthesis pathway was identified and modified. The recombinant E. coli with the further overexpression of feedback-insensitive mutant EclysC* increased the ectoine titer to 2.51 g/L with a yield of 0.37 g/g in shake flasks. After the medium optimization including the carbon and nitrogen source, sodium chloride, and magnesium sulfate concentration, the ectoine titer was improved to 4.55 g/L. 115.15 g/L of ectoine with a yield of 0.23 g/g was obtained in the 5.0 L bioreactor through the optimization of substrate feeding and IPTG supplementation in the fed-batch fermentation. To achieve the cost-effective production of ectoine, lignocellulosic hydrolysate from wheat straw was adopted. 134.08 g/L of ectoine with a yield of 0.33 g/g sugar and a productivity of 3.7 g/L/h was finally produced, representing a relatively high level of ectoine production from renewable resources compared to other studies. This study provides valuable insights into a cost-effective and efficient method for industrial-scale ectoine production.
研究领域:
生物技术
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
PROTEIN SCIENCE
·
2024
·Vol. 33
·期 10
·e5160
·
IF 4.5
Shuai Xu,Zong-Lin Li,Zhi-Min Li,Hong-Lai Liu
L-cysteine is an essential component in pharmaceutical and agricultural industries, and synthetic biology has made strides in developing new metabolic pathways for its production, particularly in archaea with unique O-phosphoserine sulfhydrylases (OPSS) as key enzymes. In this study, we employed database mining to identify a highly catalytic activity OPSS from Acetobacterium sp. (AsOPSS). However, it was observed that the enzymatic activity of AsOPSS suffered significant feedback inhibition from the product L-cysteine, exhibiting an IC 50 value of merely 1.2 mM. A semi-rational design combined with tunnel analysis strategy was conducted to engineer AsOPSS. The best variant, AsOPSS A218R was achieved, totally eliminating product inhibition without sacrificing catalytic efficiency. Molecular docking and molecular dynamic simulations indicated that the binding conformation of AsOPSS A218R with L-cys was altered, leading to a reduced affinity between L-cysteine and the active pocket. Tunnel analysis revealed that the AsOPSS A218R variant reshaped the landscape of the tunnel, resulting in the construction of a new tunnel. Furthermore, random acceleration molecular dynamics simulation and umbrella sampling simulation demonstrated that the novel tunnel improved the suitability for product release and effectively separated the interference between the product release and substrate binding processes. Finally, more than 45 mM of L-cysteine was produced in vitro within 2 h using the AsOPSS A218R variant. Our findings emphasize the potential for relieving feedback inhibition by artificially generating new product release channels, while also laying an enzymatic foundation for efficient L-cysteine production.
研究领域:
生物化学
·
酶学
·
合成生物学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2023
·
IF 22.1
Songliang Liu,Hugang Zhang,Hongjie Yu,Kai Deng,Ziqiang Wang,You Xu,Liang Wang,Hongjing Wang
Metallene with unusual physicochemical properties is a very promising class of two-dimensional electrocatalyst for efficient electrochemical hydrogen production . However, the controllable construction of metallene in the extension direction remains a great challenge. Herein, PdRh bimetallenes (PdRh BMs) and PdRh bimetallene nanoribbons (PdRh BNRs) were controllably prepared by solvent-induced strategy for efficient hydrogen evolution. The overpotential required for PdRh BNRs to reach 10 mA cm –2 at 1 M KOH is only 32 mV, which is better than PdRh BMs and Pd metallene nanoribbons. Aberration-corrected HRTEM revealed abundant defects on the surface of PdRh BNRs, which not only act as high active sites but also generate strong strain to significantly reduce the d -band center of Pd. This work provides guidance for the construction of high efficiency catalysts in metallene by applying defect engineering and strain engineering, and also provides a novel strategy for the controllable extension of metallene in longitudinal dimension.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
Sustainable Energy & Fuels
·
2022
·Vol. 6
·期 24
·5521-5530
·
IF 6.813
Dongxue Guo,Mengya Zong,Zhe Zhao,Cunzheng Fan,Danhong Wang
Seawater electrolysis under alkaline conditions represents a sustainable approach to the mass production of carbon-neutral hydrogen energy. However, the lack of efficient electrocatalysts restricts the development of this technology. Herein, core–shell-structured NiMoO4@NiFe LDH arrays with simultaneous interface and reconstruction engineering are reported as an efficient OER electrocatalyst for alkaline seawater electrolysis. The catalyst's open and hierarchical architecture, along with the excellent synergistic effects, allows for abundant active sites, high intrinsic activity and effective charge transfer. Specifically, it only required low overpotentials of 251 and 349 mV to attain current densities of 100 and 500 mA cm−2, respectively, along with impressive long-term durability over 80 h and nearly 100% faradaic efficiency. Various ex situ and in situ techniques substantiate the deep reconstruction behavior of the electrocatalyst, including fast dissolution of MoO42− anions and in situ generation of active NiFeOOH species. This work highlights that interface and reconstruction engineering are efficient strategies to fabricate advanced OER electrocatalysts.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
NANO
·
2021
·
IF 1.556
T. Jiang,X. Meng
Surface and interface engineering have shown broad application prospect in energy conversion. Mo 2 C-C/Sb 2 S 3 composites have been synthesized by coupling Mo 2 C-C composites and Sb 2 S 3 nanowires with coupling agent. The performance of the devices has been investigated. Under irradiation by light source, the device showed better electrical contact, fast response speed (rise time 0.135 s, decay time 0.132 s) and larger on/off ratio ( 1 . 5 × 1 0 2 ) than the device which assembled by mechanical mixing ( 1 × 1 0 2 ) and pure Sb 2 S 3 nanowires (47), respectively. The performance has been enhanced by modifying the surface and interface of materials. This approach provides a new idea to enhance the high-performance photodetectors and other inventive optoelectronic devices.
研究领域:
材料科学
·
光电子学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）