ACS Omega
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2026
·
IF 5.2
Jinling Bai,Yike Han,Mengting Fu,Mengting Cui,Zhiyu Liu,Zhixin Ouyang,Liyun Kong,Xinyan Dong
Steviol glycosides (SGs), particularly rebaudioside A (RA) and stevioside (STV), are the primary natural sweeteners in Stevia rebaudiana and are increasingly utilized as zero-calorie sucrose substitutes. Although water is environmentally friendly, its poor selectivity limits efficient extraction of SGs, while conventional organic solvents raise environmental concerns. Auxiliary techniques such as ultrasound-assisted extraction often require high energy input or specialized equipment. Natural deep eutectic solvents (NADES) offer a sustainable, tunable alternative for SGs extraction. In this study, the Conductor-like Screen Model for Real Solvent (COSMO-RS) was applied to screen 403 DES combinations, revealing that NADES with strong hydrogen-bonding abilities significantly enhance SGs extraction. Experimental validation identified a betaine- propionic acid NADES (1:2 molar ratio) with 45 wt % water as optimal, achieving yields of 98.15 ± 2.62 mg/g RA, 59.83 ± 1.60 mg/g STV, and 7.16 ± 0.45 mg/g rebaudioside C (RC). To address challenges in SGs recovery from NADES, static adsorption/desorption experiments were conducted using AB-8 resin. Hydrophilic NADES were effectively recovered with deionized water washing and maintained over 85% extraction efficiency after two reuse cycles. Target SGs were efficiently desorbed with 70% ethanol, while impurities were removed with 30% ethanol. The resulting yields of RA, STV, and RC were 90.56%, 86.68%, and 65.89%, with corresponding purities of 53.23%, 29.06%, and 4.42%, respectively. These results demonstrate that the combination of the betaine-propionic acid NADES and AB-8 resin offers an effective and sustainable strategy for the extraction and purification of SGs from S. rebaudiana.
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Advanced Optical Materials
·
2026
·
IF 7.2
Xuetao Yan,Lifei Chen,Yuze Ren,Kaixuan Cui,Tianliang Li,Lixing Lin,Zeyu Li,Haicheng Song,Yingying Chen,Zhenzhen Li,Lingyan Feng
Circularly polarized luminescence (CPL) materials are highly sought after for their unique chiroptical properties, but their rational design remains challenging. To address this, a machine learning (ML) platform that intelligently screens deep eutectic solvents (DES) for crafting G-quartet-based gels with tailored CPL is established. An ML classifier trained on experimental data predicts CPL activity with 0.916 accuracy. SHapley Additive exPlanations (SHAP) analysis identifies key DES molecular descriptors, guiding the discovery of novel materials that exhibit a record-high luminescence dissymmetry factor (g lum ) of 0.222 for nucleotide-based systems and achieve chirality inversion via DES modulation. The Sure Independence Screening and Sparsifying Operator (SISSO) algorithm further yields interpretable design formulas. Leveraging the distinct polarization of these gels, we demonstrate a fourth-level anti-counterfeiting strategy, decrypting concealed information via Morse and ASCII codes to enhance information security. This ML-driven methodology accelerates the discovery of high-performance CPL materials and opens new design avenues.
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Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Hu Lu,Li Yuanbin,Li Shanshan,Wu Chengyu,Wang Zhenyuan,Zhang Jichuan,Huang Chujie,Zhang Jiaheng
Chronic oxidative stress and inflammation accelerate skin aging by inducing structural compromise in intracellular components and extracellular matrix (ECM) degradation. Plant-derived bioactive peptides have attracted great attention for their outstanding biological activities. However, before protein hydrolysis, the traditional extraction strategy of protein was unsustainable for its polluting solvents and reagents. Tricholoma matsutake ( T. matsutake ) is rich in proteins, vitamins, poly-peptides and double-chain polysaccharides. Several natural deep eutectic solvents (NaDESs) were synthesized for the efficient extraction of protein from T. matsutake . The highest yield of protein (52.5 ± 0.66%) was obtained from extraction at 35 of matrine/1,2-Propanediol (MaPd) concentration for 2 h, a solid-liquid ratio of 40 mL/mg. Furthermore, the optimal peptides were prepared by protein hydrolysis with 20% alkaline protease at 55 °C for 90 min. Notably, T. matsutake peptides were good in scavenging radicals and in decreased levels of IL-1α, IL-6. Meanwhile, T. matsutake peptides exhibited anti-skin aging ability by preventing the secretion of MMP-1 and MMP-3, degradation of collagen I and elastin. The study demonstrates that NaDES combined with enzymolysis extraction is a suitable, green technique with high potential of peptides from T. matsutake . Therefore, T. matsutake peptides can be considered for developing crude drug formulations and functional food or cosmetics materials with anti-inflammatory and Anti-aging properties.
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AQUACULTURE
·
2025
·
IF 3.9
Luyao Xu,Jiamin Li,Ao Luo,Jiujie Zhou,Samwel M. Limbu,Jialing Xu,Peiyan Lu,Tingting Li,Xiaoxuan Qi,Mo Peng
To comprehensively investigate effects of dietary l -carnitine (LC) on growth performance, lipid metabolism, oxidative stress, mitochondrial function and endoplasmic reticulum (ER) stress in farmed fish fed high-carbohydrate-diets (HCD), 960 rice field eels (17.09 ± 0.14 g) were chosen and randomly assigned to 16 cages. These fish were fed a control diet (15 % α-starch, CON), a high-carbohydrate-diets (35 % α-starch, HCD), a HCD supplementing with 1.2 ‰ LC (HC/LLC), and a HCD supplementing with 2.4 ‰ LC (HC/HLC). Results of 8-week feeding trial showed that HCD increased liver lipid content, induced liver dysfunction, oxidative stress and ER stress, but not affected growth performance. Dietary LC supplementation significantly increased weight gain rate and specific growth rate and decreased liver lipid content. In the meantime, LC reduced the activity of serum ALT, improved liver's antioxidant capacity, thereby mitigating liver damage. Dietary LC positively regulated expression of genes related to oxidative stress, such as keap1 , nrf2 , cat and sod . Furthermore, LC supplementation upregulated expression of genes related to the mitochondrial function, such as crls1 and lclat1 (cardiolipin metabolism) , pgc1α, pparα and cpt1 (mitochondrial β-oxidation) . Intriguingly, administering LC-fortified diets to fish resulted in downregulation of ER stress-related gene expression, such as grp78, ire1 and atf6 . It is first reported in this study that the incorporation of LC into the diet of HCD-fed fish facilitates lipid metabolism through concurrent enhancement of mitochondrial function, reduction of oxidative stress, and alleviation of ER stress.
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AQUACULTURE
·
2026
·
IF 4.4
Yanyu Zhang,Chenhao Hu,Jingchuan Liu,Sirong Jiang,Qian Liu,Xin Zhou,Yiling Liu,Feibiao Song,Jian Luo
High-protein feeding is widely used in carnivorous marine fish to sustain growth, but it increases dependence on costly fishmeal. In practice, protein sparing by increasing dietary lipid levels leads to inconsistent results, as lipid utilization is limited by mitochondrial fatty acid oxidation (FAO) capacity, potentially inducing adiposity instead of growth. Here, golden pompano ( Trachinotus blochii ) were subjected to bidirectional FAO modulation for 90 days using mildronate (THP, FAO inhibition) or l -carnitine (L-CN, FAO enhancement). Growth performance, tissue composition, plasma metabolites, histology, and integrated liver-muscle metabolomics-transcriptomics were analyzed to assess tissue-specific metabolic remodeling. Both FAO inhibition and enhancement improved final body weight and specific growth rate. FAO inhibition promoted adiposity, reduced muscle protein and glycogen, increased lactic acid/LDH and oxidative damage, and induced liver depletion of carnitine/acylcarnitines with accumulation of TCA intermediates. In contrast, FAO enhancement reduced lipid deposition, enhanced lipid catabolism, and promoted protein retention and glycogen storage. Multi-omics analysis further showed that liver remodeling reflected carbon-flux reallocation between FAO, TCA activity and glycogen deposition, whereas muscle remodeling shifted between FAO inhibition-associated catabolic/stress responses to FAO enhancement-associated anabolic and proteostasis programs. Overall, our results demonstrate that FAO capacity, not only lipid supply, determines the shift toward protein accumulation or adiposity in golden pompano, and reveal a coordinated yet tissue-specific metabolic remodeling in liver and muscle that supports effective protein sparing while reducing reliance on costly marine protein resources.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2023
·
IF 5.2
Yixuan Zheng,Weiwei Ning,Quanhua Wang,Xueling Wei,Xingyang Li,Meng Pan
Hierarchical ZSM-5 was successfully prepared using l -carnitine and l -lysine as mesoporous templates, and the influence of the dosage of “secondary template” represented by l -carnitine on the formation of hierarchical zeolite was investigated in detail. Powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), nitrogen adsorption-desorption, nuclear magnetic resonance (NMR), thermogravimetric-derivative thermogravimetry (TG-DTG), pyridine infrared and temperature programmed desorption of ammonia (NH 3 -TPD) were used to characterize the structural and textural properties of the as-prepared samples. The results showed that the hydrophilic property of the amino acid template overcame the phase separation and constructed a mesoporous system with an ultra-small size of 2–10 nm in the as-synthesized zeolite crystals. Not only the morphology and grain size, but also the Si/Al ratio and then the acidity of zeolite were affected by the “secondary template”. Catalytic cracking of triisopropylbenzene was chosen as a probe reaction so as to investigate the effect of the fabricated hierarchical porous system on the catalytic performances of the catalysts. The results showed that the introduction of hierarchical pores elevated the external surfaces of the catalyst and therefore significantly improved the catalytic cracking performance of the catalyst for the heavy molecules triisopropylbenzene. At the same time, the introduced mesopore structure also gave the macromolecule reactant a hierarchically cracking process because of the acid active sites located in the different spaces.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Xu Zhang,Xiaotong Wang,Rui Shi,Xiaoku Ran,Xiahong He,Deqiang Dou
Ethnopharmacological relevance Red ginseng (RG), a processed product of ginseng (GS), is a generally used qi -tonifying medicine in Traditional Chinese Medicine (TCM). According to the TCM principle, RG is also generally applied to spleen-deficiency syndrome (SDS) clinically for its warmer property. However, the effective substances and mechanism of RG on SDS have not been well investigated. Aim of the study The aim of this study was to explore the effective substances and their mechanism of RG on SDS. Materials and methods The SDS model was established with a compound factor method involving an irregular diet, excessive fatigue and sennae folium with a bitter-cold property. The medicine of RG was split by multi-mode separation methods and analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS). The appearance indexes such as body weight, body temperature, swimming endurance, urine output, and water content of fecal were determined. The biochemical indexes such as D-xylose, SP , VIP and AChE in the digestive system, CRH, ACTH, CORT, E, T3, T4, T, E2 and 5-HT in the endocrine system, CS, NCR , IDH1 , COX and Na + -K + -ATPase in the metabolism of substance and energy, cAMP and cGMP in the cyclic nucleotide system were analyzed by Enzyme-linked immunosorbent assay (ELISA) kits and biochemical kits. The serum metabolites were analyzed by UPLC-QTOF/MS. Furthermore, the gut microbiota and short-chain fatty acids (SCFAs) in feces were analyzed by 16S rRNA sequencing and headspace gas chromatography-mass method. Results The pharmacological experiments showed that total saponin fraction (RGTSF), less polar fraction (RGLPF), and polysaccharides faction (RGPSF) significantly modulated the “brain-gut” axis-related indexes (the levels of VIP, AChE, and 5-HT). Besides, RGTSF also significantly modulated the hypothalamic-pituitary-adrenal (HPA) axis-related indexes as well as the substance and energy metabolism-related indexes (the levels of ACTH, CORT, A, Na + -K + -ATPase, COX, NCR and CS). RGPSF also significantly modulated the hypothalamus-pituitary-thyroid (HPT) axis-related indexes (the levels of T3 and T4). Secondly, metabolomics indicated that RGTSF could significantly regulate the abnormal metabolic pathways associated with the development of SDS, which involved steroid hormone biosynthesis, taurine and hypotaurine metabolism, primary bile acid biosynthesis, and amino acid metabolism. Subsequently, the study of gut microbiota indicated that RGLPF could increase the diversities of the gut microbiota and the relative abundance of Firmicutes in rats with SDS, while RGWEF significantly increased the relative abundance of Bacteroidetes . At the genus level, RGLPF could increase the relative abundance of Lactobacillus in rats with SDS and decrease that of Akkermansia . Meanwhile, the water-eluted fraction (RGWEF) showed a stronger regulation in SCFAs. Conclusion It is for the first time that the effective substances of red ginseng on spleen-deficiency syndrome were studied systematically, and the different mechanisms of the RG fractions involved in substance and energy metabolism as well as the “brain-gut” axis were revealed. The present study demonstrated that RGTSF, RGPSF, and RGLPF were the effective substances of red ginseng for ameliorating spleen-deficiency syndrome, indicating that ginsenosides composed of primary and secondary saponins as well as polysaccharides were the main effective substances for red ginseng in ameliorating spleen-deficiency syndrome.
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高影响因子
Food & Function
·
2022
·Vol. 14
·期 2
·1024-1036
·
IF 6.317
Weili Li,Haiyang Li,Lujuan Zheng,Jing Xia,Xiaoxuan Yang,Shuhan Men,Ye Yuan,Yuying Fan
Skeletal muscle insulin resistance is the main cause of type 2 diabetes, and mitochondria play a key role. Ginsenoside CK is the main active compound of ginseng with a variety of therapeutic effects, but few studies have reported on its mechanism towards skeletal muscle insulin resistance. Here, we found that CK significantly increased skeletal muscle insulin sensitivity, thereby alleviating hyperglycemia and insulin resistance. Furthermore, the effects of CK on skeletal muscle were associated with an improved mitochondrial fusion/fission dynamics balance and fatty acid oxidation. In fatty acid (FA)-induced C2C12 cells, CK promoted the translocation of GLUT4 to the cell membrane to improve glucose uptake and glycogen synthesis and also enhanced the mitochondrial quality. CK ameliorated the damaged mitochondrial membrane potential (ΔΨm), which was based on mitophagy activation. After the knockdown of mitophagy-related receptors, we found that DRP1/PINK1 was the key pathway of CK-induced mitophagy. These findings indicated that ginsenoside CK is a promising lead compound against diabetes.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Chenhui Gu,Xuewen Hu,Baixi Shan,Xiaojing Wu,Jun Chen
Er-Miao-Wan formula (EMW), composed of Phellodendri Chinensis Cortex and Atractylodis Rhizoma, is widely used in the treatment of hyperuricemia (HUA), gout, and related complications as a classic compound formula. However, its mechanisms for the treatment of HUA still need to be further systematically investigated. The study aimed to perform modern analytical techniques to elucidate the mechanisms of EMW in improving the symptoms of HUA from the perspective of metabolomics. We used a high-fructose diet to establish a rat model of HUA to evaluate the effects of EMW on improving HUA. Next, we established a targeted metabolomics analysis method to quantitatively analyze purine metabolites in plasma by using ultra-high-performance liquid chromatography with ultraviolet and triple quadrupole mass spectrometry (UHPLC-UV-QQQ MS), and combined with plasma non-targeted metabolomics analysis by using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q/TOF MS) to clarify the potential mechanisms of EMW to improve HUA. Oral administration of EMW could significantly increase the urinary uric acid and decrease the serum uric acid, and exhibited a remarkable effect on improving HUA. Plasma targeted metabolomics analysis showed that six purine metabolites related to HUA, including uric acid, hypoxanthine , xanthine , deoxyadenosine , deoxyguanosine , and deoxyinosine , were changed in the EMW-treated group. Further, principal component analysis (PCA) and partial least squares discrimination analysis (PLS-DA) showed that the mechanism of EMW interfering with purine metabolic pathway in the rats with HUA could be different from that of allopurinol . On the basis of plasma non-targeted metabolomics, PCA and orthogonal partial least squares discriminant analysis (OPLA-DA) screened and identified 23 potential biomarkers in the rats with HUA, and 11 biomarkers showed a trend of reversion after the intervention of EMW. The pathway analysis suggested that EMW might have therapeutic effects on the rats with HUA via the metabolic pathways including phenylalanine metabolism, glycerophospholipid metabolism, and tryptophan metabolism. In this study, a plasma targeted metabolomics method that can simultaneously quantify nine purine metabolites in rats with HUA was established for the first time, which can be used to study diseases closely related to HUA. In addition, we further explored the overall effect of EMW on HUA in combination with the metabonomic method established by non-targeted metabolomics, which was helpful to solve the defect that the pharmacological mechanism caused by multi-components and multi-targets of traditional Chinese medicine was difficult to explain scientifically and comprehensively. In summary, EMW could effectively alleviate the symptoms of high-fructose-induced HUA, and the study provided a reference for the potential therapeutic mechanism of EMW.
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TOXICOLOGY IN VITRO
·
2023
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IF 3.2
Jing Nie,Jiabei Zhou,Yi Shen,Ruimiao Lin,Haihong Hu,Kui Zeng,Huichang Bi,Min Huang,Lushan Yu,Su Zeng,Jing Miao
The widespread use of triazole fungicides in agricultural production poses a potential risk to human health. This study investigates the interaction of five triazole fungicides, i.e., tebuconazole, triticonazole, hexaconazole, penconazole, and uniconazole with human renal transporters, including OAT1, OAT3, OCT2, OCTN1, OCTN2, MATE1, MATE2-K, MRP2, MDR1, and BCRP, using transgenic cell models. For uptake transporters, triticonazole was the substrate of OAT1 and OAT3 and the inhibitor of OCT2. Tebuconazole and penconazole inhibited OCTN2 (100 μM), while tebuconazole, triticonazole, hexaconazole, penconazole, and uniconazole inhibited MATE1 (100 μM). Tebuconazole and hexaconazole inhibited MATE2-K (100 μM). All five triazole fungicides were not substrates or strong inhibitors of MRP2, MDR1, and BCRP efflux transporters. Penconazole inhibited OCT2 with IC 50 = 1.12 μM. Penconazole and uniconazole inhibited MATE1 with IC 50 = 0.94 μM and 0.87 μM. Tebuconazole and hexaconazole inhibited MATE2-K with IC 50 = 0.96 μM and 1.04 μM, indicating that triazole fungicides may inhibit renal drug transporter activity at low concentrations. Triticonazole was transported by OAT1 and OAT3, and the K m values of triticonazole were 5.81 ± 1.75 and 47.35 ± 14.27, respectively. Tebuconazole and uniconazole were transported by OAT3, and the K m values of tebuconazole and uniconazole were 30.28 ± 7.18 and 87.61 ± 31.70, respectively, which may induce nephrotoxicity.
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Frontiers in Pharmacology
·
2023
·
IF 5.6
Yang Yang,Cuiting Lin,Qiang Zheng,Leqi Zhang,Yongmei Li,Qinghua Huang,Ting Wu,Zean Zhao,Lu Li,Jian Luo,Yanqing Jiang,Qun Zhang,Xing Wang,Chenglai Xia,Jianxin Pang
Hyperuricemia (HUA) is associated with left ventricular remodeling (LVR) and thereby causes the initiation and development of a large number of cardiovascular diseases. LVR is typically accompanied by cardiomyocyte energy metabolic disorder. The energy supply of cardiomyocytes is provided by glucose and fatty acid (FA) metabolism. Currently, the effect of HUA on cardiomyocytic FA metabolism is unclear. In this study, we demonstrate that UA-induced cardiomyocyte injury is associated with cytoplasmic lipid deposition, which can be ameliorated by the FA metabolism-promoting drug L-carnitine (LC). UA suppresses carnitine palmitoyl transferase 1B (CPT1B), thereby inhibiting FA transport into the mitochondrial inner matrix for elimination. LC intervention can ameliorate HUA-associated left ventricular anterior wall thickening in mice. This study showed that FA transport dysfunction plays is a critical mechanism in both cardiomyocytic injury and HUA-associated LVR and promoting cytoplasmic FA transportation through pharmacological treatment by LC is a valid strategy to attenuate HUA-associated LVR.
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CHEMICO-BIOLOGICAL INTERACTIONS
·
2023
·
IF 5.1
Yaodong Yi,Hengbin Zhang,Mingyang Chen,Binxin Chen,Yingchun Chen,Ping Li,Hui Zhou,Zhiyuan Ma,Huidi Jiang
Doxorubicin (DOX) has been widely used to treat various tumors; however, DOX-induced cardiotoxicity limits its utilization. Since high accumulation of DOX in cardiomyocytes/mitochondria is the key reason, we aimed to clarify the mechanisms of DOX uptake and explore whether selectively inhibiting DOX uptake transporters would attenuate DOX accumulation and cardiotoxicity. Our results demonstrated that OCTN1/OCTN2/PMAT (organic cation/carnitine transporter 1/2 or plasma membrane monoamine transporter), especially OCTN2, played crucial roles in DOX uptake in cardiomyocytes, while OCTN2 and OCTN1 contributed to DOX transmembrane transport in mitochondria. Metformin (1–100 μM) concentration-dependently reduced DOX (5 μM for accumulation, 500 nM for cytotoxicity) concentration and toxicity in cardiomyocytes/mitochondria via inhibition of OCTN1-, OCTN2- and PMAT-mediated DOX uptake but did not affect its efflux. Furthermore, metformin ( iv : 250 and 500 mg/kg or ig : 50, 100 and 200 mg/kg) could dose-dependently reduce DOX (8 mg/kg) accumulation in mouse myocardium and attenuated its cardiotoxicity. In addition, metformin (1–100 μM) did not impair DOX efficacy in breast cancer or leukemia cells. In conclusion, our study clarified the role of multiple transporters, especially OCTN2, in DOX uptake in cardiomyocytes/mitochondria; metformin alleviated DOX-induced cardiotoxicity without compromising its antitumor efficacy by selective inhibition of multiple transporters mediated DOX accumulation in myocardium/mitochondria.
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危险品化学品经营许可证（带存储）