A bimodal gigaporous anion-exchange medium with the ion-exchange capacity of 0.215 mmol/ml was successfully developed based on hydrophilic gigaporous polystyrene (HGPS) microspheres (diameter between 60 and 115 μm). There are two types of pore in the medium, i.e., gigapores (0.3–7 μm) and mesopores (10–60 nm). The presence of gigapores in the medium can induce convective flow of mobile phase within microspheres, therefore contributing to high column permeability (3.55×10 −10 m 2 ), high dynamic adsorption capacity and high protein resolution at high flow velocities. The mixture of hemoglobin, transferrin and ovalbumin can be baseline separated within 3 min at 2529 cm/h. All the results indicate that anion-exchange medium as developed is very promising for large scale high-speed protein chromatography.
The temperature-sensitive inverse macroporous membranes containing thermo-responsive poly (N-isopropylacrylamide) (PNIPAM) were prepared by a template of silica microspheres. The inverse membranes were characterized by SEM, FTIR and contact angle measurements. The PNIPAM containing inverse macroporous membranes had different pore diameters at different temperatures and could be used for separation. The conductivity was measured to characterize the temperature-responsive behavior and permeability of this inverse macroporous membranes. Due to variable pore sizes, the membranes exhibited specific temperature-responsive permeability. This strategy presented here is a simple, low-cost, and controllable alternative for fluidic applications, such as the separation of particles.
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