BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Tao Wang,Zhen Yang,Chong Lei,Jian Lei,Yong Zhou
A Dynabeads-labeled magnetic immunoassay (MIA) has been developed by using an integrated giant magnetoimpedance (GMI) biosensor for the detection of alpha-fetoprotein (AFP). The GMI biosensor (Cr/Cu/NiFe/Cu/NiFe/Al 2 O 3 /Cr/Au films) integrated magnetic sensing elements and a biomolecular immunoplatform. Au film was modified with 11-Mercaptoundecanoic acid (11-MUA) for the immobilization of AFP monoclonal antibody . Double antibody sandwich immunoassay was used to specifically capture and label AFP antigen. Functionalized Dynabeads were conjugated to AFP antigen by streptavidin–biotin binding assay. GMI responses were measured for sensitive detection of AFP from 1 to 10 ng/ml. Our results revealed that the presence of AFP on the biosensor improved the GMI effect owing to the induced magnetic dipole of superparamagnetic Dynabeads, and the GMI ratio was greatly increased at high frequency. Specificity of MIA was tested through the use of 1% bovine serum albumin (BSA). The underlying biophysical mechanisms responsible for the enhanced GMI effect in the detection of AFP were discussed. This work provides a complex lab-on-chip MIA for the detection of biomarker, which may open up a new way for the development of GMI-based MIA in clinical trials .
研究领域:
生物医学工程
·
分子诊断学
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 24
·3636-3642
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IF 1.9
Zhen Yang,Huanhuan Wang,Xinwei Dong,Hailong Yan,Chong Lei,Yongsong Luo
Herein, a novel method for the detection of the cardiac biomarker myoglobin (Mb) is proposed by using an integrated giant magnetoimpedance (GMI) based immunosensor. The GMI sensor integrates magnetic sensing elements and a gold film. Micro-patterned GMI sensing elements were prepared from a cobalt-based commercial amorphous ribbon (Metglas® 2714A) using micro-electro-mechanical system (MEMS) technology. The gold film was deposited on the GMI sensing element to act as a support for the immunoplatform. The classical sandwich assay was used for the detection of Mb targeted with Dynabeads by using the antibody–antigen pair combination of biotin–streptavidin. The GMI ratios of the sensors with different concentrations of the antigen against Mb were investigated. As a result, the GMI ratios are significantly improved at high frequency if Mb is present. The sensor has a linear response in the 1 to 10 ng ml−1 concentration range, and the detection limit is 0.5 ng ml−1 at a working frequency of 10 MHz. The method is successfully applied to the determination of Mb, and can be used to detect other cardiac markers like Troponin and CK-MB.
研究领域:
生物医学工程
·
临床检验
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Xuecheng Sun,Chong Lei,Lei Guo,Yong Zhou
Herein we report a new separable detection system combining magnetic nanoparticle labels with giant magnetoresistance (GMR) first time developed for Escherichia coli (E. coli) O157H:H7 detection. The GMR sensor fabricated employing standard microfabrication technique, while double antibody sandwich immunoassay and streptavidin–biotin binding assay were employed for immobilizing and labeling E. coli O157H:H7 on Au-film coated glass wafer. The GMR-based immunosensor detected E. coli O157H:H7 antigens at different sample concentrations with a minimum detectable limit of 100 CFU/mL, well accepted for early detection in clinical diagnosis and environmental monitoring. Compared with the recently reported GMR sensor based detection methods, this newly developed separable detection method possesses several advantages such as convenient manipulation, avoid being contaminated and damaged by chemical solution and reusability without cleaning. So it is of considerable interest towards the biomedical application based on known specific binding of target and labels.
研究领域:
生物医学工程
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微生物学
·
传感技术
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 13
·2008116
·
IF 18.808
Li Yang,Hao Wu,Liang Zhang,Wenfeng Zhang,Luying Li,Tappei Kawakami,Katsuaki Sugawara,Takafumi Sato,Gaojie Zhang,Pengfei Gao,Younis Muhammad,Xiaokun Wen,Boran Tao,Fei Guo,Haixin Chang
The discovery of type-II Weyl semimetal states in layered transition metal dichalcogenides Td-WTe 2 indicates great potential for novel electronic, spintronic, and quantum devices. Theoretically, the interaction between the topological states and the magnetic ordered states of Td-WTe 2 enables the modulation of Weyl semimetal states by an external magnetic field. However, currently, ferromagnetism in layered Td-WTe 2 is still elusive and rarely observed. In this research, ferromagnetic order into WTe 2 using magnetic chromium (Cr) doping with a two-step Te flux strategy is introduced. The Curie temperature ( T c ) and the ferromagnetic moment are well tuned with a Cr doping concentration. The T c of the Cr-doped layered Td-WTe 2 could be regulated from 182 to 283 K, which is close to room temperature. The saturation magnetic moment could be changed from 2.26 to 4.20 emu g –1 , which is stronger than most values reported for these materials. Most intriguingly, the Cr-doped layered Td-WTe 2 single crystals still exhibit semimetallic behavior and they possess very large magnetoresistance with obvious Shubnikov de Haas (SdH) quantum oscillations and an anomalous Hall effect. The findings offer feasible ways to induce and tune ferromagnetic orders in layered Td-WTe 2 and thus to control its topological phase with external magnetic fields.
研究领域:
凝聚态物理
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材料科学
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自旋电子学
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量子材料
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拓扑物态
✓ 已验证
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Qiaoge Bayin,Lei Huang,Chunhui Ren,Yusheng Fu,Xing Ma,Jinhong Guo
Since December 2019, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused millions of deaths and seriously threatened the safety of human life; indeed, this situation is worsening and many people are infected with the new coronavirus every day. Therefore, it is very important to understand patients’ degree of infection and infection history through antibody testing. Such information is useful also for the government and hospitals to formulate reasonable prevention policies and treatment plans. In this paper, we develop a lateral flow immunoassay (LFIA) method based on superparamagnetic nanoparticles (SMNPs) and a giant magnetoresistance (GMR) sensing system for the simultaneously quantitative detection of anti -SARS-CoV-2 immunoglobulin M (IgM) and G (IgG). A simple and time-effective co-precipitation method was utilized to prepare the SMNPs, which have good dispersibility and magnetic property, with an average diameter of 68 nm. The Internet of Medical Things-supported GMR could transmit medical data to a smartphone through the Bluetooth protocol, making patient information available for medical staff. The proposed GMR system, based on SMNP-supported LFIA, has an outstanding advantage in cost-effectiveness and time-efficiency, and is easy to operate. We believe that the suggested GMR based LFIA system will be very useful for medical staff to analyze and to preserve as a record of infection in COVID-19 patients.
研究领域:
医学诊断
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生物传感技术
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传染病研究
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 4
·1-14
·
IF 5.479
Feng Zhu,Zhi Shaotao,Guo Lei,Zhou Yong,Lei Chong
The authors describe an integrated microfluidic chip for immunodetection of the prostate specific antigen (PSA) by using giant magnetoimpedance (GMI) sensor. This chip contains an immunoreaction platform and a biomarker detection system. The immunoreaction platform contains an incubation chamber and a reactive chamber to implement immunological reaction in microfluidics. The system can detect PSA rapidly with ultra-high sensitivity. Both are fabricated by MEMS technology. Immunomagnetic beads (If PSA binds to its antibody (that is labeled with immunomagnetic beads; IMBs) it will be trapped on the surface of self-assembled film. Trapped IMBs generate a stray magnetic field under the magnetization of the external applied magnetic field and can be detected by the GMI sensor. The chip can detect PSA with a detection limit as low as 0.1 ng ∙ mL −1 and works in the 0.1 ng ∙ mL −1 to 20 ng ∙ mL −1 concentration range. Compared to established GMI biosensors, the magnetic microfluidic chip reduces assay time, and lends itself to fast detection. It also avoids complex handling steps, enhances reaction efficiency and decreases experimental errors.
An integrated magnetic microfluidic chip which contains immunoreaction platform and biomarker detection system was designed and microfabricated by micro-electromechanical systems (MEMS) technology to detect prostate specific antigen (PSA) rapidly, and has promise in Point-of-care (PoC) diagnostic applications.
研究领域:
生物医学工程
·
诊断学
·
微纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 63
·51330-51336
·
IF 3.84
Tao Wang,Lei Guo,Chong Lei,Yong Zhou
Herein, a giant magnetoimpedance (GMI) sensor combined with the sandwich immunoassay was used for ultrasensitive detection of carcinoembryonic antigens (CEA). Oblong-shaped Au films were enclosed with SU-8 photoresists to form several microcavities that were treated with different concentrations of CEA. The quantification of low-concentration CEA (1 pg mL−1–10 ng mL−1) was achieved by using one single GMI sensor, demonstrating a good linear relationship between the GMI response and the CEA concentration. The lower detection limit for CEA was found to be as low as 1 pg mL−1, which provides extreme sensitivity. We observed that high-concentration CEA (>10 ng mL−1) can result in a cluster of Dynabeads, thereby reducing the target signals since the adjacent magnetic dipole fields canceled each other out. Target specificity of the GMI-based magnetic immunoassay has also been verified by testing nonspecific targets. The results obtained here suggest that the GMI sensor could serve as an ultrasensitive immunosensor for quantitative determination of biomarkers.
研究领域:
生物传感器
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医学诊断
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材料科学
·
免疫学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 15
·2411-2417
·
IF 3.741
Yang Zhen,Liu Yan,Lei Chong,Sun Xue-cheng,Zhou Yong
We report on a method for the determination of magnetic bead-labeled C-reactive protein (CRP), a biomarker of cardiovascular diseases and inflammations. It is using a flexible giant magnetoimpedance (GMI)-based platform. Micro-patterned GMI sensing elements were prepared from a cobalt-based commercial amorphous ribbon (Metglas® 2714A) using micro electro-mechanical system (MEMS) technology. A gold film was then deposited on the GMI sensing element to act as a support for the immuno platform. Sandwich assays are performed using antibody-antigen combinations and biotin-streptavidin interactions on the gold film substrate surface via self-assembled layers. The GMI ratios of the sensors with different concentrations of antigen against CRP were investigated. The results show that the presence of CRP antigens on the biosensor improves the GMI effect owing to the induced magnetic dipole of superparamagnetic beads, and that the GMI ratios show distinct changes at high frequency. This bioassay for CRP has a linear detection range between 1 to 10 ng·mL −1 . This new method in our perception provides a widely applicable basis for rapid diagnostic testing and will pave the way for future development of electrochemical point-of-care diagnostic devices for cardiac diseases.
(a) Graphical illustration of CRP test setup. (b) Magnetic field arrangement of the beads under an applied magnetic field. (c) GMI changes in relation to the concentration of the CRP
研究领域:
生物医学工程
·
材料科学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2020
·
IF 4.189
Jingyi Cai,Wenxuan Wang,Duo Pan,David P. Young,Hongbo Gu,Zhanhu Guo
The barium ferrite/polyaniline (BaFe12O19/PANI) nanocomposites with room temperature negative giant magnetoresistance (GMR) are fabricated by the surface-initiated polymerization (SIP) method. The maximum negative GMR value is −7.1% in BaFe12O19/PANI nanocomposites with a BaFe12O19 loading of 20 wt % at a magnetic field of 9 T. The electrical transport mechanism of these samples is investigated by thermally activated transport (TAT) model at the high temperature range (180–290 K) and Mott variable range hopping (VRH) mechanism at the low temperature range (50–180 K). The results reveal that the electrical transport of pure PANI and BaFe12O19/PANI nanocomposites obeys the 3-D VRH mechanism. The estimated activation energy (E) (related to the energy required for charge carrier hopping process) for pure PANI and BaFe12O19/PANI nanocomposites with a BaFe12O19 loading of 10, 20, 30, and 40 wt % is calculated to be 38.5, 47.0, 52.1, 53.8, and 66.8 meV, respectively, according to the TAT model. With the aim of explaining the negative GMR phenomenon in BaFe12O19/PANI nanocomposites, the forward interference model is introduced, demonstrating the localized length of carriers varies with the applied magnetic field.
研究领域:
材料科学
·
纳米技术
·
电气工程
·
物理学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 6
·1831-1837
·
IF 4.831
Yang Zhen,Liu Yan,Lei Chong,Sun Xue-cheng,Zhou Yong
We report on a method for ultrasensitive detection and quantification of the pathogen Escherichia coli ( E. coli ), type O157:H7. It is using a tortuous-shaped giant magnetoimpedance (GMI) sensor in combination with an open-surface microfluidic system coated with a gold film for performing the sandwich immunobinding on its surface. Streptavidin-coated super magnetic Dynabeads were loaded with biotinylated polyclonal antibody to capture E. coli O157:H7. The E. coli -loaded Dynabeads are then injected into the microfluidics system where it comes into contact with the surface of gold nanofilm carrying the monoclonal antibody to form the immunocomplex. As a result, the GMI ratio is strongly reduced at high frequencies if E. coli O157:H7 is present. The sensor has a linear response in the 50 to 500 cfu·mL −1 concentration range, and the detection limit is 50 cfu·mL −1 at a working frequency of 2.2 MHz. In our perception, this method provides a valuable tool for developing GMI-based microfluidic sensors systems for ultrasensitive and quantitative analysis of pathogenic bacteria. The method may also be extended to other sensing applications by employing respective immunoreagents.
Fig. (a) Graphical illustration of the test setup. (b) Relationship of GMI signal vs. E. coli O157:H7 concentration.
研究领域:
生物医学工程
·
微流控技术
·
传感技术
·
病原体检测
·
磁感测技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）