Journal of Nanomaterials
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2020
·
IF 1.98
Xinxin Li,Jinku Xu
In this paper, three kinds of ZnS NPs capping different amount of mercaptoethanol (ME) were synthesized, and the effect of capping amount on ZnS NPs was studied, indicating that the capping amount of ME on ZnS surface decreases, while ZnS size increases as the decrease of added capping agent in synthesis process, and ZnS NP capping middle amount of ME (~27.7 wt%) shows a highest apparent refractive index (RI) value. ZnS NPs were composited into polymeric matrices by a simple “one-step” thermocuring method characterized by adding a small amount of functional monomer of glycidyl methacrylate (GMA) and solvent of N,N-dimethylformamide (DMF) The reaction mechanism, studied by 1H NMR spectra, indicates that the epoxy of GMA monomer can be easily opened and chemically grafted on ME-capped ZnS surface under the catalysis of DMF, and then copolymerized with other monomer. By the route, ZnS NPs can be composited into pure poly(N,N-dimethylacrylamide)-type (DMA-type), DMA-type copolymer, and DMA-free matrices to fabricate transparent films, and its RI value can be improved by either optimizing capping amount on ZnS surface or increasing ZnS content in the nanocomposites. The RI value of resulting dried nanocomposites can be improved to 1.764 by compositing 80 wt% ZnS capping middle amount of ME.
研究领域:
材料科学
·
化学工程
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Analytical Methods
·
2018
·Vol. 10
·期 26
·3256-3262
·
IF 2.073
Pan Li,Jiawei Li,Minghong Bian,Danqun Huo,Changjun Hou,Ping Yang,Suyi Zhang,Caihong Shen,Mei Yang
In this paper, we developed a label-free fluorescence assay employing BSA-Au NCs, S2O32−, and 2-ME for the highly selective and sensitive detection of Pb2+ in sorghum, river water and tap water. When the BSA-Au NCs reacted with S2O32− in solution, Au(S2O3)23− complexes were formed immediately on the surfaces of BSA-Au NCs. After adding Pb2+ and 2-ME, the BSA-Au NCs rapidly dissolved to form Au–S complexes. As a result, the fluorescence intensity decreased dramatically, allowing quantification of Pb2+ in the aqueous solution. This reaction is highly specific under optimal detection conditions. A good linear relationship between the fluorescence intensity and the concentrations of Pb2+ could be obtained in the range of 10 to 1000 nM (R2 = 0.9910), with a detection limit (LOD; S/N ratio = 3) down to 1.043 nM. Significantly, compared with other studies, we greatly shortened the reaction time by substantial improvements in materials. On the basis of the above-mentioned work, we successfully immobilized S2O32− modified BSA-Au NCs on wax-printed papers to achieve the purpose of simple and real-time field monitoring of Pb2+. We picked up R-values (the red value of the RGB color model) from captured flurescence images under UV light (365 nm) and acquired a linear correlation between R-values and Pb2+ concentrations. This linear correlation exhibits the great potential of this approach. The proposed probe also showed satisfactory selectivity as well as excellent reproducibility. Multiple real sample experiments showed the tremendous prospect of this method, as well.
研究领域:
分析化学
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环境科学
·
食品科学
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DESIGNED MONOMERS AND POLYMERS
·
2017
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IF 1.139
Miao Sun,Chunxiao Yin,Yanan Gu,Yun Li,Zhirong Xin
A series of tertiary amine-based hyperbranched poly(amine-ester)s have been synthesized by Michael addition polymerization of trifunctional monomer, TMEA and difunctional monomer, diacylates in chloroform, and the resultant polymers were subsequently treated with mercaptoethenol or 1-dodecanethiol for improving stability in storage. The caption efficiency of mercaptoethanol is much better than that of 1-dodecanthiol. Kinetic study reveals that the thiol group is consumed faster than the acrylate group when the polymerization with feed molar ratio of diacrylate/TMEA = 2/1 was carried out. At initial polymerization, monomer conversion increases fast, but the molecular weights increase slowly and sharp increase of the molecular weight occurs at the final polymerization. The hyperbranched polymers were well characterized by 1H NMR spectra and TD-SEC, and DBs of the polymers obtained are between 0.6 and 0.82, as well as the molar ratios of diacrylate/TMEA in the hyperbranched polymers are between 1.60 and 1.82. The fluorescence efficiency and quantum yields of HypET20, HypHT24 and HypDT24 has the following sequence: HypET20 > HypHT24 > HypDT24.
研究领域:
高分子化学
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材料科学
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PubMed
高影响因子
MICROCHIMICA ACTA
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2018
·Vol. 185
·期 3
·1-8
·
IF 5.705
He Liuying,Lu Yuexiang,Wang Feiyang,Gao Xinxin,Chen Ying,Liu Yueying
The authors describe a colorimetric method for the determination of Hg 2+ ions based on the inhibition of the activity of the enzyme urease. The pH value of solution increases when urease hydrolyzes urea, which can be visualized by adding a pH indicator such as Phenol Red (PhR). Mercaptoethanol as a typical thiol is added to the system to improve selectivity because it binds metal ions and then - unlike the Hg 2+ mercaptoethanol complex - does not inhibit urease. Hence, the color of the pH indicator PhR turns from yellow to pink as the solution becomes alkaline. The Hg 2+ mercaptoethanol complex, in contrast, strongly inhibits urease and the color of the solution remains yellow. The findings were used to design a photometric assay based on the measurement of the ratio of absorptions of PhR at 558 nm and 430 nm. It has a linear response over the 25 to 40 nM Hg 2+ concentration range and a 5 nM detection limit. This is well below the guideline values of Hg 2+ specified by the US Environmental Protection Agency and the World Health Organization for drinking water (10 nM and 30 nM, respectively). The method was employed to the determination of Hg 2+ in water samples spiked with 10 nM levels of Hg 2+ where color changes still can be observed visually.
Schematic presentation of a colorimetric method for the ultrasensitive detection of Hg 2+ based on the inhibition of urease activity. Mercaptoethanol is used to improve the selectivity. Even at Hg 2+ concentrations as low as 5 nM, the color change still can be easily observed by bare eyes.
研究领域:
分析化学
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环境科学
·
生物化学
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高影响因子
CHINESE JOURNAL OF CHEMICAL PHYSICS
·
2024
·Vol. 37
·期 4
·563-570
·
IF 1.2
Shi Hehang,Ye Xiaodong
Two soy protein 11S fractions with different surface sulfhydryl contents were prepared. Utilizing analytical ultracentrifugation, the effects of storage time and hydrogen peroxide at different concentrations (0.5—100 mmol/L) on the two 11S fractions were investigated. Results show that after removing 2-mercaptoethanol (2-ME) by size exclusion chromatography, the 11S fraction with high surface sulfhydryl content (2.0 mol sulfhydryl/mol 11S) progressively formed 15S and 21S in dilute solutions during storage at 4 °C for 82 days. While, the 11S fraction with low surface sulfhydryl content (0.2 mol sulfhydryl/mol 11S) was stable under the same condition. Moreover, after treating the 11S with high surface sulfhydryl content with 1 mmol/L H 2 O 2 , the weight percentage of 15S reached the maximum value of 20%. The 15S induced by air and H 2 O 2 could be totally converted to 11S with the addition of 10 mmol/L 2-ME, which could be attributed to that the disulfide bond linking two 11S molecules is on the surface of the 15S and easily accessible to the reducing agent 2-ME. This study helps us to deeply understand the formation mechanism of 15S and the stability of 11S.
研究领域:
食品科学
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蛋白质化学
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分析化学
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NEUROCHEMICAL RESEARCH
·
2016
·Vol. 41
·期 11
·2890-2903
·
IF 2.472
Han Jun,Zhang Hai,Wang Sheng,Zhou Jun,Luo Yi,Long Li-Hong,Hu Zhuang-Li,Wang Fang,Chen Jian-Guo,Wu Peng-Fei
Sulfhydryl compounds such as dithiothreitol (DTT) and β-mercaptoethanol (β-ME) are widely used as redox agents. Previous studies in our group and other laboratory have reported the effect of sulfhydryl compounds on the function of glutamate receptor, including plasticity. Most of these findings have focused on the N -methyl- d -aspartic acid receptor, in contrast, very little is known about the effect of sulfhydryl compounds on α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor (AMPAR). Here, we observed that DTT (100 μM), β-ME (200 μM) and l -cysteine (200 μM) significantly elevated the surface expression of AMPARs via reducing their palmitoylation in rat hippocampal slices in vitro . Increased surface stability of AMPARs was not be correlated with the altered redox status, because the chemical entities containing mercapto group such as penicillamine (200 μM) and 2-mercapto-1-methylimidazole (200 μM) exhibited little effects on the surface expression of AMPARs. Computing results of Asp-His-His-Cys (DHHC) 3, the main enzyme for palmitoylation of AMPARs, indicated that only the alkyl mercaptans with chain-like configuration, such as DTT and β-ME, can enter the pocket of DHHC3 and disrupt the catalytic activity via inhibiting DHHC3 auto-palmitoylation. Collectively, our findings indicate a novel redox-independent mechanism underlay the multiple effects of thiol reductants on synaptic function.
研究领域:
神经科学
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药理学
·
细胞生物学
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FOOD CHEMISTRY
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2021
·
IF 7.514
Fengya Wang,Xiaobo Zhang,Changxin Huangfu,Mingzhen Zhu,Chunsheng Li,Liang Feng
Dicofol is an organochlorine pesticide, which is widely used in fruits, tea and other crops, and is moderately toxic to humans. Therefore, the monitoring of organochlorine pesticide-dicofol is critical for food safety. In this work, a fluorometric chemosensor based on mercaptoethanol and boron dipyrromethene (BODIPY) was first constructed to detect the dicofol. The chemosensor displayed turn-off fluorescence behavior upon dicofol with a detection limit of 200 ppb. The nucleophilicity of the glutathione and other biological thiols was studied to evaluate the reactivity of thiols with dicofol. In practical applications, an obvious color difference was observed on a paper based microfluidic device modified by phenyltriethoxysilane (PTES). We designed an integrated device for pretreatment and paper-based detection, and successfully used for the detection of dicofol in tea. The applicability was demonstrated by detection of dicofol in real tea samples with good recovery ranging from 86% to 109%. The apparatus was convenient and could be used for on-site evaluation of dicofol.
研究领域:
分析化学
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环境科学
·
食品安全
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PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Jiamei Fu,Mengqiu Quan,Haiquan Sun,Junyi Zhou,Qingyi Xie,Minghui Cui,Xiaolin Wang,Fenglong Li,Jin Zhu,Jing Chen
In the field of sports medicine, especially in high-intensity sports such as football, basketball, and skiing, ligament injuries are extremely common. However, current artificial ligaments face multiple critical limitations, including suboptimal biocompatibility, limited host tissue integration, insufficient mechanical durability to withstand physiological stresses, and inadequate durability during long-term cyclic loading. To address these challenges, 1-(2-Hydroxyphenyl)-3-phenyl-2-propenone (HCC), which shared structural similarity with naturally occurring chalcones, is employed as the raw material for the chain extender, combined with HMDI and polycaprolactone diol (PCL diol), to synthesize a novel polyurethane (HCMPU). The results showed that the maximum stress of HCMPU-3 reached 42.1 MPa, with an elongation at break of up to 710%. It also demonstrated outstanding durability, maintaining structural integrity without significant degradation after 5000 loading cycles. Additionally, during the initial implantation period, its stability in an enzymatic environment can provide strong support to the human body before new tissue regeneration, making it a promising candidate for an ideal artificial ligament. Moreover, the favorable biocompatibility of HCMPUs is demonstrated through cytotoxicity testing and rat pathological staining sections. Therefore, this work demonstrated the potential of HCMPU to overcome key limitations in current artificial ligaments and advance the development of next-generation materials for ligament repair.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yuan Tang,Xiaojuan Lei,Yan Liu,Mengcong Liu,Lin Lei,Kaihong Zeng,Jian Ming
This study investigated interactions between capsaicin (CAP), piperine (PIP), allicin (ALL) and grass carp myofibrillar protein (MP) regarding protein conformational changes. While the bioactivities of these pungent compounds are recognized, a systematic understanding of their diverse binding patterns and consequent conformational remodeling of MP remains limited. The binding patterns and regulatory mechanisms were systematically evaluated through multispectral analysis, physicochemical characterization, and molecular docking simulations. Results demonstrated that CAP binding coincided with MP aggregation, increased surface hydrophobicity, and elevated random coil content, alongside the weakest binding energy (−6.9 kcal/mol). These effects were consistent with a binding pattern dominated by hydrophobic interactions and dynamic quenching. PIP binding correlated with conformational loosening and maintained colloidal dispersibility but limited stabilization, associated primarily with hydrogen bonding and static quenching (K q > 9.0 × 10 12 L/(mol·s)). ALL binding at higher concentrations corresponded to conformational compaction, increased α-helix/β-sheet content, and enhanced structural stability, with the strongest binding affinity (−19.82 kcal/mol). These changes aligned with a mechanism involving covalent bonding synergized by hydrogen/hydrophobic interactions. These findings provide molecular-scale insights into pungent spice-MP interactions, offering strategies for optimizing spicy food systems.
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高影响因子
Current Protocols
·
2025
·Vol. 5
·期 10
·e70241
·
IF 2.2
Yiming Zhao,Jingjun Hong
Chromatin remodeling is a process of regulating gene expression levels by altering chromatin structure, facilitated by chromatin remodeling factors. ISWI (Imitation SWItch), a type of chromatin remodeling factor, plays a crucial role in epigenetic regulatory processes, such as cell differentiation and development. It primarily achieves remodeling effects by promoting the direct sliding of nucleosomes and their relocation on DNA. To study the function and structural characteristics of ISWI, this basic protocol describes a simple and rapid procedure for the large-scale expression and purification of soluble Ct ISWI 77-1038 and Ct ATPase 77-722 proteins in Escherichia coli ( E. coli ). The procedure provides high yields (∼20 mg) and is followed by in vitro measurement of ATP hydrolysis activity. © 2025 Wiley Periodicals LLC. Basic Protocol : Expression, purification, and ATP hydrolysis activity measurement of Ct ISWI and Ct ATPase
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Amel Thanina Amrouche,Minghao Xu,Shengyang Ji,Chen Qi,Nesma S. Abdelaty,Baiyi Lu
Edible flowers are valued for their bioactive, nutritional, and aesthetic properties. This study explored the 3D printability of five edible flowers and identified the essential role of their native biopolymers through integrated analyses of composition, functionality, viscoelasticity, and structure. Heat treatment significantly enhanced printing precision in pectin- and protein-rich gels. The most substantial improvement occurred in pectin-rich roselle (59.96 % to 99.81 %) and protein-rich lotus (90.63 % to 96.25 %). Higher printability was linked to biopolymer-network rearrangement via hydrogen and disulfide bonds and electrostatic interactions, which reduced free water and lowered cellulose crystallinity (up to 11.00 %). These changes improved gel network flexibility, increased storage modulus, and improved microstructural regularity, resulting in smoother layer deposition. In contrast, cellulose-rich gels lacking sufficient pectin and protein underwent recrystallization (4.84 %) and densification after heating, reducing viscoelasticity and printability. These findings provide mechanistic insights for optimizing edible-flower gels for 3D food printing.
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Horticulturae
·
2025
·Vol. 11
·期 11
·1292
·
IF 3
Hu Zhao,Xiaopu Shi,Zilu Zhao,Juan Wang,Baul Ko,Jong Hyang Bae,Yang Gyu Ku,Ho Cheol Kim,Chul Min Kim
Young leaves and sprouts ofToona sinensisare widely consumed throughout Asia. However, they are highly perishable, resulting in a short shelf life and limited marketability. Low-temperature storage can extend shelf life but is energy-intensive, restricting large-scale use. Salicylic acid (SA) exhibits preservative properties, representing a promising alternative. In this study, we investigated the effects of 250 μmol SA treatment on postharvestT. sinensisbuds and leaves stored at 20 °C for 7 days and compared it to low-temperature storage (4 °C). SA treatment delayed wilting, reduced water loss, suppressed total soluble solid accumulation, and inhibited active oxygen free radical and malondialdehyde production compared to the untreated controls at room temperature while outperforming low-temperature storage. The treated buds also maintained higher antioxidant enzyme activity and preserved non-enzymatic antioxidant compounds, including ascorbic acid, polyphenols, and flavonoids. Although total amino acid content steadily increased under SA treatment, individual amino acids greatly fluctuated. SA treatment reduced nitrate reductase and glutamate dehydrogenase activities, as well as NO2•−levels, indicating its effects on nitrogen metabolism. Our findings indicate that SA improves the postharvest quality ofT. sinensisbuds during room-temperature storage, providing a theoretical basis for applying SA as a storage agent forT. sinensisbuds.
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