JOURNAL OF HAZARDOUS MATERIALS
·
2015
·
IF 4.529
Yao Wang,Wentao Zhu,Jing Qiu,Xinru Wang,Ping Zhang,Jin Yan,Zhiqiang Zhou
In the present study, the enantioselective metabolism, cytotoxicity of hexaconazole and its influence on tryptophan metabolism in rat hepatocytes in vitro were investigated. Following the exposure of primary rat hepatocytes to rac -hexaconazole, the concentrations of its enantiomers in the media were determined by chiral high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). The half-lives ( t 1/2 ) of (+)-hexaconazole and (−)-hexaconazole were 5.17 h and 19.80 h, respectively, indicating that the metabolic process was enantioselective with (−)-hexaconazole enrichment. Using the MTT method, the EC 50 values of rac -hexaconazole, (+)-hexaconazole and (−)-hexaconazole after 12 h of exposure were determined to be 71.62, 62.71 and 67.94 μM, respectively. Tryptophan metabolism was monitored using metabolomics profiling techniques. Hexaconazole and its enantiomers caused the down-regulation of tryptophan levels and the up-regulation of kynurenine (KYN) levels, suggesting a role for hexaconazole in the activation of the KYN pathway and providing information for the mechanism of its toxicity.
研究领域:
毒理学
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分析化学
·
药理学
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DOI
PubMed
高影响因子
Surfaces
·
2025
·Vol. 8
·期 4
·84
·
IF 2.9
Quanping Diao,Liyang Sun,Linlin Lv,Tiechun Li,Jiaqi Pan,Weiwei Luo
Hexaconazole, a triazole-class fungicide, demonstrates broad-spectrum protective and therapeutic activity against fungal pathogens, particularly those from Basidiomycota and Ascomycota, such as brown spot and powdery mildew. Despite its efficacy in controlling Actinidia brown spot disease in kiwifruit, excessive hexaconazole residues pose significant health risks due to its high toxicity. To address this challenge, a rapid analytical method for detecting hexaconazole residues in kiwifruit was developed using surface-enhanced Raman spectroscopy (SERS). The methodology employed silver colloid (C-AgNPs) as the active substrate and 1 mol/L NaCl as the aggregation agent, optimized through systematic testing, resulting in an optimal volume ratio of 400:225 between C-AgNPs and hexaconazole solution and a sequential mixing order of C-AgNPs + NaCl + Hexaconazole, followed by a 20 min incubation period. The characteristic Raman peak at 1584 cm−1was identified as the spectral signature for hexaconazole quantification. Analytical validation revealed a linear detection range of 0.25–2.25 mg/L (R2= 0.9870), precision with a relative standard deviation (RSD) of 1.7%, and an average recovery rate of 88.40–105.50%, confirming the method’s robustness. This approach enables rapid, non-destructive analysis with minimal sample pretreatment, offering high sensitivity and stability. This method demonstrates great potential for detecting hexaconazole residues in agricultural products.
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高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Zhiqin Huang,Qingli Zeng,Dan Li,Laisheng Li
The new type of bis- triazolyl bridged cyclodextrin-based stationary phase (BCDP) was prepared through “Click reaction”. BCDP had high selectivity for triazoles and was used for the simultaneous fast separation and determination of enantiomeric residues (flutriafol, hexaconazole and diniconazole) in fruits and vegetables by high-performance liquid chromatography (HPLC). The QuEChERS method assisted by magnetic nanoparticles was used for sample pretreatment, and then a gradient elution was carried out by using MeOH-H 2 O as the mobile phase on BCDP to achieve rapid resolution of the triazole enantiomers within 25 min. After condition optimization, simultaneous baseline separations of the six enantiomers were achieved with resolutions of 1.54 (flutriafol), 2.27 (hexaconazole) and 1.97 (diniconazole). A new HPLC quantification method of the six enantiomeric residues in apples, tomatoes and cucumbers was established by an internal standard with a photo-diode array (PDA) detector at 210 nm. The results showed that the excellent linear relationships of all triazole enantiomers were observed in the concentration range of 0.5–50 mg L -1 ( R 2 = 0.9986–0.9999). The average recoveries of enantiomers in fruits and vegetables were from 91.95 % to 109.92 % with RSDs of 0.97 %–1.32 % for intraday and 1.30 %–3.03 % for interday. The limits of detection (LODs, S/N = 3) and limits of quantitation (LOQs, S/N = 10) of all enantiomers were less than 0.03 mg kg −1 and 0.10 mg kg −1 , respectively. BCDP had high selectivity for triazole due to its synergistic inclusion with triazoles and advantage in fast and simultaneous separation of multiple triazole enantiomers, which could save analysis time and reduce solvent consumption. BCDP was a new separation material for the scientific evaluation of chiral pesticides in the food safety analysis.
研究领域:
食品安全分析
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农药残留检测
·
分析化学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Lin Li,Xiao-ting Yuan,Zhi-guo Shi,Lan-ying Xu
Chiral stationary phases (CSPs) have always been research hotspot in enantiomer separation. Currently, most of the CSPs are based on silica platform. In this research, monodisperse, porous glycidyl methacrylate-divinylbenzene copolymer particles (poly(GMA-DVB)) were designed and prepared. Then the GMA was further reacted with ethylenediamine to introduce amino groups onto the polymer, which provide anchoring sites for cellulose derivatives. Herein, Cellulose-tris (3,5-dimethylphenylcarbamate) (CDMPC) was successfully coated onto the polymer microspheres, achieving a stable and successful CSP. The porous structure and the surface moieties of the CSPs were studied in detail. The chromatographic separation was optimized. Hexaconazole,methyl DL-mandelate,benzoin and tebuconazole have been successfully separated on the CSP column, with column efficiency as high as 10,200 plates/m, which is comparable with some silica-based CSPs. The research has indicated that the poly(GMA-DVB) is a promising candidate for constructing CSPs for chiral separation.
研究领域:
分析化学
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色谱学
·
材料科学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Zhongxuan Hu,Changlong Shu,Meijing Wang,Tingze Yang,Hongling Pei,Teng Wang,Siyu Sun,Fang Zhang
Developing environmentally friendly pesticides and improving pesticide utilization efficiency are urgent needs for modern agriculture. Herein, a pH-responsive biodegradable dual pesticide-fertilizer was fabricated using extracts from naturally occurring Protaetia brevitarsis larval frass (PBLF) material. Iron ions (Fe 3+ ) were integrated as metal nodes into PBLF, and hexaconazole (Hex), a triazole fungicide, was encapsulated in Fe-PBLF with a loading rate of 22.93 %. Hex@Fe-PBLF nanoparticles (NPs) presented a sphere-like construction with a hydrated size of 715.3 ± 5.0 nm and exhibited acid-responsive controlled release behavior, gradually self-degrading in acidic environments within 120 h. The NPs showed good foliar adhesion and were effectively absorbed and translocated by pathogenic fungal mycelium and the rice seedling. Compared with Hex Technical control (TC), the control effects of Hex@Fe-PBLF against Rhizoctonia solani ( R. solani ) and Botrytis cinerea ( B. cinerea ) increased by 9.9 % and 12.73 %, respectively, after 7 days. This naturally sourced nanocarrier not only promoted the growth of rice seedlings as a fertilizer but also enhanced their stress resistance. The low mobility of Hex@Fe-PBLF NPs in soil effectively reduced environmental risks. The excellent biosafety of the Fe-PBLF nanocarrier was further evaluated. Overall, this environmental-friendly multifunctional nano pesticide-fertilizer has promising application potential in agriculture.
研究领域:
农业科学
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纳米技术
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植物病理学
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环境科学
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Yuxin Wang,Jin Liu,Suzhen Li,Jizhen Fu,Xiaowen Wang,Li Li,Xu Jing
The widespread use of triazole fungicides has raised concerns over residual. This study presents an automated, environmentally friendly, simple, and efficient method for detecting triazole fungicides in food samples, utilizing high-throughput liquid processing, dispersive liquid-liquid microextraction, and ultra-high-performance liquid chromatography-tandem mass spectrometry. The automated liquid handling workstation was employed to automate the whole sample pre-treatment process. Fatty acid, bio-based solvent, and saturated sodium chloride solution were used as the extractant, dispersant, and demulsifier, respectively. The whole operation process obviated the use of toxic reagents and eliminated the need for time-consuming centrifugation. The linear ranged from 0.01 to 1 μg L −1 with limits of detection and quantification of 0.003 μg L −1 and 0.01 μg L −1 , respectively. Ten triazole fungicides were successfully detected in food samples with recoveries of 70.1 %–105.7 %. The automation of dispersive liquid-liquid microextraction demonstrates huge potential for broad applicability within the field of food safety testing.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Longhui Luo,Dongmei Chen,Xiufang Yan,Chao Kang,Wei Tian,Qian Liu,Xiaoying Yang,Tingting Chen,Youhua Long
Triazole pesticides are widely used to control pathogenic fungi in agricultural production, but the irrational use of these pesticides will have many negative effects on human health and ecological environment. In this study, we constructed a relatively complete visual monitoring system for triazole pesticides based on surface-enhanced Raman spectroscopy (SERS). Silver nanoparticles (Ag@ICNPs) modified with iodide and calcium ions showed stronger SERS enhanced ability and better reproducibility, and the characteristic peaks of eight different triazole pesticides were captured simultaneously. Principal component analysis (PCA) and support vector machine (SVM) were used to identify these pesticides. The developed method has strong quantitative analysis ability, excellent detection sensitivity and wide detection range, and has been successfully applied to the determination of pesticide residues in fruits and vegetables. In addition, dynamic SERS imaging technology combined with multivariate curve resolution-alternating least squares (MCR-ALS) to visually monitor the penetration behavior of triazole pesticides could remove the influence of interfering substances, thus obtaining an accurate distribution map of pesticides, which promoted the quantification of pesticide penetration process. The diffusion ability of different systemic pesticides into fruits and vegetables was revealed. Electrochemical (EC) assisted SERS of triazole pesticides showed that potential-induced molecular reorientation of pesticide molecules on the electrode surface was induced by the polarization of active substrate, which significantly improved the SERS signal of pesticides. Overall, this systematic pesticide monitoring study not only provides an effective innovative scheme for controlling the detection of hazardous substances in agricultural products, but also brings significant prospects for food safety guarantee.
研究领域:
食品安全
·
分析化学
·
农业科学
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产品关联
DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 15
·3130
·
IF 4.6
Jun Li,Jianlan Deng,Qian Tao,Chenyu Yan,Yuxuan Liu,Jianxiao Yang,Zhong Cao
Luminescent metal–organic frameworks (MOFs) are used for the detection of organophosphorus pesticides (OPs) due to their large surface area and pore volume as well as their special optical properties. However, most self-luminescent MOFs are not only complex to synthesize and unstable in water but also feature a “turn-off” sensing system, which has highly restricted their practical applications in OP detection. Herein, a “turn-on” fluorescence sensor based on the guest-induced luminescence MOF Ru(bpy)32+@UiO-66 was constructed, which realized the sensitive detection of OPs through a dual-enzyme system for the first time. Compared with self-luminescent MOFs, Ru(bpy)32+@UiO-66 was not only more easily synthesized but also had higher chemical and photostability in water. In this strategy, by means of the hydrolysis of AChE and ChOx, H2O2will be produced, which can oxidize Fe2+to Fe3+, thereby quenching the fluorescence of Ru(bpy)32+@UiO-66. In the presence of OPs, the activity of AChE can be inhibited, resulting in the inability to generate H2O2and Fe3+, which will turn on the fluorescence signal of Ru(bpy)32+@UiO-66. As a result, the Ru(bpy)32+@UiO-66 sensing system not only had high sensitivity for OPs detection but also possessed a satisfactory detection recovery rate for parathion-methyl in real samples, which provides a new approach for OP detection in food safety as well as environmental monitoring.
研究领域:
分析化学
·
材料科学
·
环境科学
·
生物化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Xiaofei Han,Jia Chen,Zhan Li,Kaijun Quan,Hongdeng Qiu
A magnetic porous carbon (Fe 3 O 4 @PC) was successfully fabricated based on combustion combined with solvothermal method , and then used as magnetic solid-phase extraction absorbent for the determination of four triazole fungicides . The porous carbon was fabricated by imperfect combustion of filter paper loaded with zinc nitrate hexahydrate, which possessed extremely abundant pores, high specific surface area (908.05 m 2 g −1 ) and large pore volume . Due to the introduction of solvothermal method used to prepare Fe 3 O 4 @PC, homogeneously distributed magnetic nanoparticles and high saturation magnetization were obtained. Compared with another magnetic porous carbon (Fe 3 O 4 @C), Fe 3 O 4 @PC obviously enhanced enrichment factors and extraction recoveries. Several key parameters influencing extraction efficiency were optimized. The satisfactory enrichment factors, low limits of detection of the method and good linearities were achieved under the optimal conditions. The relative recoveries ranged from 82.8% to 113.2% in three spiked real samples with relative standard deviations lower than 6.2%. The results indicated the great potential of the novel Fe 3 O 4 @PC as adsorbent for the extraction of triazole fungicides from complex real samples.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Chi Wu,Kai He,Huizhen Li,Lan Zhang,Liangang Mao,Lizhen Zhu,Jinhua Jiang,Xingang Liu
The transgenerational impacts of pesticide mixture on Daphnia magna ( D. magna ) following long-term exposure, particularly regarding transcriptomic effects, remain poorly understood. We analyzed 470 irrigation water samples from various Chinese provinces. Imidacloprid (IMI, 71.1 %), clothianidin (CLO, 60.43 %), and tebuconazole (TEB, 64.68 %) were the most frequently detected pesticides, with nearly 50 % of samples showing mixtures of TEB with IMI or CLO. D. magna were exposed to environmentally relevant concentrations (0.89–1.62 μg/L) over 12 generations. Key effects included reduced fecundity, delayed development, and smaller body size, with these impacts exacerbating across generations. A Leslie matrix model revealed declines in population size, intrinsic rates of increase, and age structure. Notably, mixed pesticide exposures induced specific pathways related to oocyte meiosis, resulting in greater toxicity than single exposures. Additionally, disrupted lipid metabolism and feeding behaviors were linked to insufficient energy for growth and reproduction, affecting ovarian development. These findings highlight the ecological risks of long-term pesticide exposure and offer insights for mitigation strategies in aquatic ecosystems.
研究领域:
生态毒理学
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环境科学
·
水生生态学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Tian-Qiong Shi,Yi-hang Shen,Ya-Wen Li,Zi-Yi Huang,Zhi-Kui Nie,Chao Ye,Yue-Tong Wang,Qi Guo
Gibberellic acid (GA3), produced industrially by Fusarium fujikuroi , stands as a crucial plant growth regulator extensively employed in the agriculture filed while limited understanding of the global metabolic network hinders researchers from conducting rapid targeted modifications. In this study, a small-molecule compounds-based targeting technology was developed to increase GA3 production. Firstly, various small molecules were used to target key nodes of different pathways and the result displayed that supplement of terbinafine improved significantly GA3 accumulation, which reached to 1.08 g/L. Subsequently, lipid and squalene biosynthesis pathway were identified as the key pathways influencing GA3 biosynthesis by transcriptomic analysis. Thus, the strategies including in vivo metabolic engineering modification and in vitro supplementation of lipid substrates were adopted, both contributed to an enhanced GA3 yield. Finally, the engineered strain demonstrated the ability to achieve a GA3 yield of 3.24 g/L in 5 L bioreactor when utilizing WCO as carbon source and feed.
研究领域:
植物科学
·
分子生物学
·
生物技术
·
代谢工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jin Liu,Yuanjun Nie,Yu Niu,Li Li,Xu Jing
A universal, green, and rapid lignin-based emulsive liquid-liquid microextraction (ELLME) method was established to detect nine triazole fungicides in water, juice, vinegar , and alcoholic beverages via UHPLC-MS/MS. By employing an environmentally friendly emulsifier (lignin), the proposed ELLME was compatible with more extractants, and not restricted to fatty acids. Due to the high amphiphilic properties and three-dimensional structure of lignin, the emulsion was quickly formed through several aspirate-dispense cycles of the green extractant (guaiacol) and lignin solution. And a micropipette was used for rapid microextraction . The limit of detection was 0.0002–0.0057 μg L −1 . The extraction recoveries and relative standard deviation were 81.7%–102.0% and 0.9%–7.1%, respectively. Finally, three green metric tools were used to verify the greenness of the whole procedure. The proposed lignin-based ELLME successfully emulsified green solvents , indicating that emerging solvents may be excellent alternatives as extractants in ELLME for pesticide residue analysis in food samples.
研究领域:
分析化学
·
环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）