MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Xiangrong Zeng,Zhongshu Duan,Yifu Zhu,Xiaoting Lei,Zhenzong Fan,Hongyu Xiao,Yangping Wen
Recent advancements in two-dimensional materials have highlighted black phosphorene (BP) as a promising candidate for optical and electrical applications. However, its practical utility is hindered by ambient instability and limited conductivity. This study aims to solve these problems with a surface passivation strategy. Silver nanoparticles (AgNPs) and rice husk biochar (RHB) are combined to prepare a BP-based nanocomposite (RHB-BP-AgNPs). AgNPs effectively occupy oxidation sites on BP’s surface, mitigating degradation while enhancing conductivity with RHB synergy. The morphology, microstructure and chemical composition of RHB-BP-AgNPs nanocomposite was comprehensively characterized by diversified techniques. The RHB-BP-AgNPs exhibited exceptional ambient stability (>16 days) and superior electrocatalytic activity, enabling its application as a sensing platform for baicalin detection. The sensor demonstrated a wide linear response (0.007–8.0 μM), a low detection limit (5.45 nM), and high selectivity against interferents. Validation in real pharmaceutical samples yielded satisfactory recovery rates of 95.4–104.6 % with RSD from 1.27 to 2.13, underscoring practical reliability. This work not only presents a robust strategy to stabilize and functionalize BP but also advances rapid, sensitive detection methodologies for bioactive compounds in traditional medicine, bridging material innovation with analytical applications.
研究领域:
材料科学
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分析化学
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DOI
高影响因子
Foods
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2023
·Vol. 12
·期 8
·1590
·
IF 5.2
Yaxian Chen,Yini Cai,Zhimin Zhao,Depo Yang,Xinjun Xu
In this study, the hot water extraction process of crude polysaccharides from Morindae officinalis radix (cMORP) was conducted and optimized through a single-factor test and orthogonal experimental design. With the optimal extraction process (extraction temperature of 80 °C, extraction time of 2 h, liquid/solid ratio of 15 mL/g, and number of extraction of 1), the cMORP was obtained by the ethanol precipitation method. The chemical properties and preliminary characterization of the cMORP were analyzed by chemical or instrumental methods. Furthermore, to indicate a preliminary study on safety, a single oral dose of 5000 mg/kg body weight (BW) was administered orally to Kunming (KM) mice for acute toxicity, and the cMORP was administered orally to KM mice once a day at doses of 25, 50, and 100 mg/kg BW for 30 days. General behaviors, body weight variations, histopathology, relative organ weights, and hematological and serum biochemical parameters were observed and recorded. The results suggested there were no toxicologically significant changes. Based on the safety study, cMORP can be initially considered non-toxic with no acute oral toxicity up to 5000 mg/kg BW and safe at up to 100 mg/kg BW in KM mice for 30 days.Keywords:Morindae officinalis radix;crude polysaccharides;hot water extraction;optimization;preliminary characterization;safety study
研究领域:
药用植物学
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药学
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毒理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Keming Jiang,Dong Huang,Dawei Zhang,Xinluan Wang,Huijuan Cao,Qian Zhang,Chunyan Yan
Scope In China, the root of M . officinalis has been widely used over thousands of years against a wide range of bone disease such as lumbago, limb-ache, sciatica and rheumatic arthralgia, and has tremendous medicinal value. But the bioactive constituents responsible for the osteoprotective effects in M . officinalis remain unknown. Methods and results M . officinalis polysaccharides were extracted, isolated and purified via DEAE-cellulose 52 and Sephacryl S-100HR column to obtain two saccharides (MOP70-1 and MOP70-2). The results of osteogenic activity assays revealed that MOP70-1 and MOP70-2 significantly promoted the proliferation, differentiation and mineralization of MC3T3-E1 cells. Furthermore, MOP70-2 also upregulated gene expression of runt-related transcription factor 2, osterix, osteocalcin, osteopontin, bone sialoprotein and osteoprotegerin, which implied that MOP70-2 stimulated osteoblastic differentiation by up-regulating osteogenic differentiation-related marker genes. In addition, structural analysis indicated that MOP70-2 contained (2 → 1)-linked- β -D-Fru f residues and terminated with a glucose residue. Morphological and conformational analyses indicated that MOP70-2 exhibited spherical structure of conglomeration and had no triple helix structure. Conclusion Our studies reported the osteogenic inulins obtained from root of M . officinalis for the first time. The systematical investigation including extraction, purification, biological activities and structural characterization provide a strong evidence for future therapeutic applications as anti-osteoporosis agent.
研究领域:
药理学
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生物化学
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中医药学
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DOI
PubMed
高影响因子
PHYTOMEDICINE
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2022
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IF 6.656
Chuan Li,Liru Tian,Yihai Wang,Huan Luo,Jia Zeng,Peiqiang Su,Shulin Chen,Zhiheng Liao,Weimin Guo,Xiangjiu He,Shuqing Chen,Caixia Xu
Background Morinda officinalis (MO) is a herb used in Traditional Chinese Medicine (TCM) for the treatment of osteoporosis. M13, a MO-based anthraquinone compound is known to suppress osteoclast activity. However, whether M13 promotes MSCs osteogenic differentiation and its potential mechanism remains unknown. Purpose To examine the influence of M13 on MSCs proliferation and osteogenic differentiation and elucidate the underlying mechanism. Methods/Study designs The effect of M13 exposure on MSCs proliferation was assessed via CCK8 assay, clone formation assay, immunofluorescence, RT-qPCR, and Western blot. The M13-mediated osteogenesis in vitro and ex vivo were evaluated via ALP and Alizarin red S staining, osteogenesis-associated gene (Runx2, Col1a1 and Opn) expression, and fetal limb explants culture. Molecular docking was employed for target signal pathway screening. The potential signaling mechanisms of M13-promoted MSCs osteogenic differentiation were analyzed by introducing XAV939 (Wnt/β-catenin signaling inhibitor). Results M13 induced certain obvious positive effects on MSCs proliferation and osteogenic differentiation. Treatment with M13 enhanced MSCs viability and clone numbers. Meanwhile, M13 promoted osteogenic gene expression, enhanced ALP intensity and Alizarin red S staining in MSCs. In terms of mechanism, M13 strongly interacted with the docking site of the WNT signaling complex, thereby activating the Wnt/β-catenin pathway. Furthermore, the M13-mediated osteogenic effect was partially inhibited by XAV939 both in vitro and ex vivo , which confirmed that the Wnt/β-catenin axis is a critical regulator of M13-induced osteogenic differentiation of MSCs. Conclusion Our study elucidated for the first time that M13 significantly promoted osteogenic differentiation of MSCs via stimulation of the Wnt/β-catenin pathway in vitro and ex vivo .Our findings offered new additional evidence to support the MO or M13-based therapy of osteoporosis.
研究领域:
药理学
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细胞生物学
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分子生物学
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传统中药
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骨生物学
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DOI
PubMed
高影响因子
Chinese Journal of Natural Medicines
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2024
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IF 4
Kun MA,Mengnan LI,Yahui SONG,Xinjing MAO,Shaolan ZHANG,Yaping ZHANG,Yuning JIANG,Chuanyuan JI,Kunxu NIU,Hongrong LI,Yunlong HOU,Cong WEI
Bazi Bushen (BZBS), a traditional Chinese medicine (TCM), has demonstrated therapeutic efficacy in testicular dysfunction within D-galactose and NaNO 2 mouse models. This study aimed to ascertain if BZBS could also mitigate the decline in testicular function associated with natural aging. Therefore, male aged mice were employed to evaluate the preventive effects of BZBS on male reproductive aging. This was achieved by assessing sex hormone production, testicular histomorphology, and spermatogenesis. Relative to the untreated aged control group, BZBS administration elevated the levels of sex hormones and spermatocyte populations and preserved normal testicular structure in aged mice. Notably, spermatogenesis was maintained. Further analyses, including malondialdehyde (MDA) assays and real-time PCR, indicated that BZBS diminished testicular oxidative stress and the inflammatory burden. Corroborating these findings, mice treated with BZBS exhibited reductions in the populations of senescent and apoptotic cells within the seminiferous tubules, suggesting alleviated cellular damage. In contrast, we observed that rapamycin, a drug known for its longevity benefits, induced excessive testicular apoptosis and did not decrease lipid peroxidation. Collectively, our results highlight BZBS’s promising clinical potential in counteracting male reproductive aging, underlining its mechanisms of action.
研究领域:
中医
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生殖生物学
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衰老研究
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药理学
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DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
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2020
·Vol. 17
·期 5
·e2000056
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IF 2.039
Shao-Qi Fu,Zi-Yuan Wang,Zheng-Meng Jiang,Zhi-Ming Bi,E-Hu Liu
Osteoporosis (OP) is a metabolic bone disease affecting nearly 200 million individuals globally. Morinda officinalis F.C. How (MOH) has long been used as a traditional herbal medicine for the treatment of bone fractures and joint diseases in China. However, it still remains unclear how the compounds in MOH work synergistically for treating OP. In this study, we used prednisolone (PNSL)-induced zebrafish OP model to screen the antiosteoporosis components in MOH. A network pharmacology approach was further proposed to explore the underlying mechanism of MOH on OP. The PNSL-induced zebrafish model validated that two anthraquinones, one iridoid glycoside, and two saccharides exerted antiosteoporotic effect. We constructed the components-targets network and obtained the enriched Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. A total of 26 candidate compounds of MOH and 257 related targets could probably treat OP through regulating osteoclast differentiation and MAPK signaling pathway. Our work developed a strategy to screen the antiosteoporosis components and explore the underlying mechanism of MOH for treating OP at a network pharmacology level. Graphical
研究领域:
药理学
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生物学
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中医药学
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系统生物学
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产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
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2022
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IF 3.204
Chao Wang,Quan-Hao Wei,Zhen-Qiang Xin,Lin-Lin Tian,Jun-Sheng Zhang,Hua Zhang
Chemical fractionation of the EtOH extract of the roots of a traditional Chinese herb, Morinda officinalis , afforded an array of methyl 2-naphthoate derivatives ( 1 – 9 ) including four pairs of enantiomers ( 1 – 4 ), two pimarane diterpenes and two ursane triterpenoids . Among them, eight compounds ( 1a / 1b - 3a / 3b , 11 and 13 ) were reported in the current work for the first time. The structures of the new compounds, including their absolute configurations, were defined by spectroscopic analyses in combination with quantum chemical electronic circular dichroism (ECD) and gauge-independent atomic orbital (GIAO) NMR calculations. All the isolates were evaluated for their inhibitory effect on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in murine RAW264.7 macrophage cells, and the enantiomers 1a and 3b exhibited moderate activity with IC 50 values of 41.9 and 26.2 μM. Meanwhile, compound 3b also dose-dependently inhibited the secretion of two pro-inflammatory cytokines TNF-α and IL-6 in the same cell model.
研究领域:
药理学
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天然产物化学
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药物化学
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免疫学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Chunyan Yan,Dong Huang,Xia Shen,Ningbo Qin,Keming Jiang,Dawei Zhang,Qian Zhang
Morinda officinalis is an important traditional tonic herbal medicine. In the present study, we found that crude polysaccharides extracted from M. officinalis , named MO90, could significantly increase the bone mineral density (BMD) of the whole femur, distal femur, and proximal femur in ovariectomized (OVX) rats. In addition, MO90 decreased the level of bone turnover markers and prevented the deterioration of trabecular microarchitecture. To investigate the fractions responsible for anti-osteoporosis activity, one novel inulin-type fructan, MOW90-1, was isolated from MOP90. Structural analysis indicated that MOW90-1 consists of a backbone of (2→1)-linked- β -D-Fru f , and is terminated with (1→)-linked- α -D-Glc p and (2→)-linked- β -D-Fru f . Furthermore, an in vitro anti-osteoporosis assay indicated that MOW90-1 promoted proliferation, differentiation, and mineralization of MC3T3-E1 cells by up-regulating the expression of runt-related transcription factor 2, osterix, osteopontin, and osteocalcin. In conclusion, our studies provide supporting evidence for future use of this novel M. officinalis fructan as a key nutrient of health products.
研究领域:
药理学
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骨骼生物学
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中药学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·Vol. 35
·期 43
·2417743
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IF 19
Junji Li,Gaohua Li,Pengfei Guan,Rikuan Tong,Haoyang Feng,Kai Ang,Zhen Kong,Zhaobo Zhu,Can Liu,Cheng Li,Hongliang Wu,Yongjian Sun
It is now understood that oxidative stress, characterized by an excess of reactive oxygen species (ROS) within growth plate injury sites, can impede cartilage repair through various mechanisms. Morinda officinalis (MO), a traditional Chinese herbal medicine, possesses potent antioxidant properties. Furthermore, extracellular vesicles (EVs) derived from plants exhibit comparable physical and chemical characteristics to their parent plants, along with favorable tissue permeability and biocompatibility. Herein, it develops a ROS-sensitive hydrogel by self-crosslinking two natural extracellular matrix (ECM)-derived components: polyvinyl alcohol (PVA) and chondroitin sulfate grafted with phenylboric acid (CSBA). The hydrogel is subsequently combined with MO-derived EVs (MO-EVs) to eliminate excess ROS from the damaged microenvironment and release MO-EVs as needed to facilitate growth plate damage healing. In vitro studies revealed that a CSBA-PVA hydrogel containing MO-EVs facilitated the restoration of chondrocyte mitochondrial function by activating the NRF2-HO-1/NQO1 pathway, thereby enhancing endogenous antioxidant capacity. Furthermore, in vivo results reveal that implantation of CSBA-PVA hydrogel loaded with MO-EVs significantly inhibited bone bridge development and facilitated cartilage restoration within the growth plate. CSBA-PVA@MO-EVs therapy brings huge prospects as a therapeutic approach, offering novel insights and therapeutic strategies for treating growth plate injuries.
研究领域:
生物医学工程
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再生医学
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植物药理学
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氧化应激生物学
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软骨组织工程
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纳米医学
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DOI
高影响因子
危险品化学品经营许可证（带存储）