INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Hua-jin Zeng,Cong-hui Cheng,Si-meng Liu,Yan Ding,Ran Yang,Ling-bo Qu
In this study, the liquid culture system of Pleurotus nebrodensis mycelium with high yield were established by using orthogonal experiments. Results indicated a 58.08 % increase in mycelium biomass and a 2.22 % increase in polysaccharide content after condition optimization. Experiments showed that the extracted polysaccharides have significant antioxidant and anti-exercise fatigue activities. They could effectively scavenge DPPH, ABTS, hydroxyl free radicals and superoxide anaion, prolong weight-loaded swimming time in mice, reduce levels of MDA, LD, and LDH in serum, enhance SOD activity, as well as increase hepatic and muscle glycogen reserves. The mechanism may be attributed to the activation of the Nrf2/Keap1 signaling pathway, inhibition of oxidative stress, and subsequent exerting anti-fatigue effects. The results in this work provides new avenue for easily accessible natural polysaccharide resources with a very convenient approach, which is beneficial for the development of anti-fatigue functional foods for public consumption.
研究领域:
食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Foods
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2022
·Vol. 11
·期 10
·1443
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IF 5.561
Hongbo Li,Suya Xie,Shangqiao Cao,Liangbin Hu,Dan Xu,Jiayi Zhang,Haizhen Mo,Zhenbin Liu
Edible fungi play an important role in material and energy cycling. This study explored the role ofPleurotus eryngiimycelia in the transformation of potato high-calorie starch to low-calorie β-glucan. First, the 3D printing performance of the potato medium was optimized. After inoculating the fermentation broth ofPleurotus eryngiiin 3D printing, we studied the microstructure and material composition of the product. Along with the increase in 3D printing filling ratio, the starch content of the culture product decreased from 84.18% to 60.35%, while the starch content in the solid medium prepared using the mold was 67.74%. The change in β-glucan content in cultured products was opposite to that of starch, and the content of the culture product increased from 12.57% to 24.31%, while the β-glucan content in the solid medium prepared using the mold was 22.17%. The amino acid composition and content of the 3D printing culture system and solid culture products prepared using the mold were similar. The 3D printing culture system promoted the bioconversion efficiency of mycelia. It also showed high application potential ofPleurotus eryngiimycelia for the preparation of low-calorie food.Keywords:bioconversion;Pleurotus eryngiimycelia;starch;3D printing;β-glucan
研究领域:
食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Kai Ye,Chujing Fu,Hengjun Du,Shiguo Chen,Donghong Liu,Gaoxing Ma,Hang Xiao
Edible mushrooms polysaccharides are widely recognized for their biological activities, yet the prebiotic potential of Pleurotus ostreatus polysaccharides (POP) remains underexplored. In this study, POP was isolated, structurally characterized, and evaluated for digestibility and impact on gut microbiota. Structural analysis revealed that POP comprises →4)-α-Glcp-(1→, →3)-β-Glcp-(1→, →4,6)-α-Glcp-(1→ and →3,4)-α-Glcp-(1→ linkages as the backbone, with side chains of 1,6-α-Galp and α-Manp residues. POP was resistant to simulated gastrointestinal digestion. During fecal fermentation, POP significantly increased microbial diversity, with Chao1 and Shannon indices rising by 26.95 % and 32.67 %. Furthermore, POP promoted the relative abundance of beneficial genera like Prevotella (8.1-fold increase), Bacteroides (3.8-fold), and Faecalibacterium (4.4-fold), while reducing opportunistic pathogens including Fusobacterium and Escherichia . Total short-chain fatty acid (SCFA) production reached 54.7 mM at 24 h, particularly acetate, propionate, and butyrate. These results highlight the prebiotic potential of POP and support its application as a functional food ingredient for improving gut health.
研究领域:
食品科学
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微生物学
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糖生物学
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营养学
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生物技术
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DOI
PubMed
高影响因子
Food & Function
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2024
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IF 5.1
Chunping Jiang,Hongyu Li,Junqi Li,Guangying Zou,Cheng Li,Zhengfeng Fang,Bin Hu,Wenjuan Wu,Xiaolin Li,Zhen Zeng,Qingying Luo,Yuntao Liu
This study investigated the physicochemical characteristics and fermentative behavior between original polysaccharides (PCPs) and polysaccharides extracted after microwave cooking (MPCPs) from Pleurotus cornucopiae during simulated digestion and fecal fermentation. The results revealed notable physicochemical differences between of PCPs and MPCPs. MPCPs exhibited a higher total carbohydrate content, with an increased proportion of glucose. Additionally, MPCPs showed a lower molecular weight (MW) and, a blue shift in Fourier transform infrared spectroscopy (FT-IR). Digestion has a minimal effect on the physicochemical and structural characteristics of PCPs and MPCPs. Within the first 6 h of fermentation, the gut microbiota showed significantly higher utilization of MPCPs. However, PCPs were consumed faster and surpassed MPCPs later. After 24 h, both PCPs and MPCPs were degraded and utilized by the gut microbiota, showing an increased abundance of Firmicutes and Bacteroidota. PCPs excelled in promoting beneficial gut microbiota, such as Phascolarctobacterium, Megamonas, and Bacteroides. Conversely, MPCPs demonstrated a stronger ability to inhibit the growth of harmful opportunistic pathogenic gut microbiota, such as Fusobacterium and Parasutterella. In addition, the content of acetic, propionic, and butyric acids increased significantly in both PCPs and MPCPs. These findings highlight the potential of Pleurotus cornucopiae polysaccharides as prebiotics for intestinal homeostasis.
研究领域:
食品科学
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微生物学
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营养学
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胃肠病学
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DOI
PubMed
高影响因子
Frontiers in Nutrition
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2024
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IF 4
Mengxin He,Qing Peng,Xiaoqing Xu,Bo Shi,Yu Qiao
Given the abundance of beneficial properties and enzymes secreted by edible oyster mushrooms, their mycelium could serve as a starter for fermented foods to enhance their nutritional and bioactive quality. This study aimed to investigate the effects on the nutritional ingredients, antioxidant activity, and non-volatile metabolites during solid-state fermentation (SSF) of soybeans by Pleurotus ostreatus mycelium. The results indicated that the contents of dietary fiber and starch in fermented soybeans decreased, while the amounts of protein and lipid increased after SSF (P < 0.05). Analysis of the total phenolic content (TPC) and antioxidant activities of the fermented soybeans revealed that the methanolic extracts significantly increased TPC and antioxidant activities against intracellular reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, as well as against DPPH and ABTS radicals in vitro. A total 154 differential metabolites were identified after SSF, and a Spearman correlation study revealed a direct relationship between antioxidant activities and certain metabolites including phenolic compounds, oligopeptides, and free fatty acids etc. Among these metabolites, phenolic compounds produced by the shikimic acid pathway were diverse in variety and had the greatest multiple differences. The study discovered that a potential mechanism involving SSF with P. ostreatus mycelium increased the antioxidant activity of soybeans.
研究领域:
食品科学
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营养学
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微生物学
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生物化学
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DOI
PubMed
高影响因子
Journal of Energy Storage
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2024
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IF 8.9
Qianghong Wu,Youzhi Wu,Fen Ran
The harm of vanadium and nano-plastic pollution to organisms and the destruction of ecological environment are increasingly concerned by the world. However, due to the multiple valence states of vanadium and the high temperature cracking of nano-plastics, which can provide additional capacity and effectively improve the structural morphology and graphitization degree of materials, so it has great development potential in energy storage field. In this work, Pleurotus ostreatus is used to absorb both vanadium and nano-plastics, including nano-PS, nano-PS-NH 2 , and nano-PS-COOH, which are used to regulate the absorption of vanadium ions by Pleurotus ostreatus. Vanadium ions, nano-plastics, and their complexes can coordinate and decompose with various polymers of Pleurotus ostreatus. The biochar-vanadium composites prepared by this method have high specific surface area, high graphitization degree, rich hierarchical porous structure, and N, O, S, and other heteroatoms, showing good spatial confinement effect and heteroatom anchoring effect. The series of optimization processes make the electrode materials exhibit excellent electrochemical performance in supercapacitors. When the current density is 0.5 A·g −1 , the specific capacitance reaches 582.6 F·g −1 ; at 50 A·g −1 , the specific capacitance is still as high as 325 F·g −1 . After 30, 000 cycles, the specific capacitance can still reach 361.6 F·g −1 at 2 A·g −1 . The Coulomb efficiency is about 99 %. In addition, the electrode material can also be applied in zinc-ion batteries.
研究领域:
环境科学
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材料科学
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能源存储
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
Current Research in Food Science
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2024
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IF 6.2
Yufei Zhang,Yanyin Lu,Dandan Pan,Yanyan Zhang,Chen Zhang,Zexin Lin
Despite lignocellulose hindering the extraction of intracellular components, tea residue can serve as an excellent substrate for fungal fermentation owing to their lignocellulose-degrading abilities. Thus, the fermentation efficiencies of Lentinus edodes , Lentinus sajor-caju (Fr.) , Flammulina filiformis , Hericium erinaceus , Pleurotus pulmonarius , and Monascus kaoliang B6 were evaluated using tea residue as a medium. P. pulmonarius and L. sajor-caju (Fr.) exhibited the fastest growth rates, with colony radii of 33.1 and 28.5 mm, respectively. M. kaoliang B6 demonstrated substantial degradation abilities for cellulose, hemicellulose, and lignin, with decolorization radii of 12.2, 0.9, and 8.5 mm, respectively. After a 9-days liquid fermentation, M. kaoliang B6 achieved the highest conversion efficiency at 27.8%, attributed to its high cellulase (191 U∙mL −1 ) and lignin peroxidase (36.9 U∙L −1 ) activities. P. pulmonarius and L. sajor-caju (Fr.) showed lower conversion rates of 8.6% and 3.8%, despite having high hemicellulase activities (67.1 and 70.9 U∙mL −1 ). Fermentation by M. kaoliang B6 resulted in a reduction of protein and total sugar content in the tea residue by 174 and 192 mg g −1 , by which the mycelium's protein and total sugar content increased by 73 and 188 mg g −1 . Co-fermentation of these three strains had little effect on the improvement of conversion efficiency, which might owe to the antagonistic interactions among the strains. Generally, utilizing tea residue for edible fungi fermentation is a sustainable process for bio-waste treatment, enabling efficient nutrient conversion under mild conditions without adding chemicals.
研究领域:
微生物学
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生物技术
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废物管理
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真菌生物学
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产品关联
DOI
PubMed
高影响因子
Fermentation-Basel
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2023
·Vol. 9
·期 4
·322
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IF 3.7
Jian Wang,Quanjin Jiang,Zhenyu Huang,Yan Wang,Hynek Roubik,Kai Yang,Ming Cai,Peilong Sun
Soybean meal is a class of by-products obtained from the processing of soybean products. Despite its high nutritional value, the presence of glycoside isoflavones limits human use of soybean meal. This study evaluated the effect of solid-state fermentation (SSF) with different edible mushroom mycelia (Pleurotus ostreatus,Hericium erinaceus, andFlammulina velutipes) on the proximate composition, antioxidant properties, and physicochemical properties of fermented soybean meal powder (SP). The results revealed that fermented SP had a higher nutritional value when compared to SP.P. ostreatuswas the most pronounced among the three species. Crude protein content was found to have increased by 9.49%, while the concentration of glutamate and aspartic acid increased by 23.39% and 23.16%, respectively. SSF process significantly increased the total polyphenol content (TPC) and aglycone isoflavone content by 235.9% and 324.12%, respectively, resulting in increased antioxidant activity (evaluated by the DPPH, •OH, ABTS+assays). Microstructural changes in fermented SP and nutrient degradation and utilization were observed. Thus, fermented SP can be used as a raw material with enhanced nutritional properties to develop new functional foods, such as plant-based foods represented by plant meat. It provides a promising approach for increasing the added value of soybean meal.Keywords:soybean meal;solid-state fermentation;edible mushrooms;nutritional value
研究领域:
食品科学
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微生物学
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营养学
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生物技术
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
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IF 3.974
Xuedan Li,Hang Ma,LingLing Li,Yufeng Gao,Yunzhen Li,Heng Xu
A pot experiment was conducted to investigate the detoxification mechanism of Agrocybe aegerita ( A. aegerita ). The physiological responses , subcellular distribution and chemical forms of cadmium (Cd) in A. aegerita grown in Cd stress were analyzed. The results showed that the biomass was decreased under Cd stress, while the production of malonaldehyde, thiols, and low-molecular-weight organic acids (LWMOAs) as well as the antioxidant enzymes in A. aegerita was increased compared with control group. The HPLC results showed that nine LWMOAs were found in A. aegerita with critic acid as the dominant and they played important role in the detoxification and accumulation of Cd in A. aegerita . More Cd was accumulated in pileus than in stipe. Differential centrifugation technique showed that the majority of Cd was compartmentalized in the soluble fraction (53–75%) and bound to the cell wall (19–42%). The proportion of Cd in the cell wall increased with the increase of the accumulation of Cd in the fruiting body, but in the soluble fraction showed an opposite trend. Furthermore, most of the Cd in A. aegerita was mainly in the forms of NaCl- (29–49%) and ethanol-extractable Cd (20–40%). The ethanol- and water-extractable Cd in stipe (60–66%) was higher than in pileus (43–49%). Thus intracellular detoxification mechanisms of Cd in A. aegerita is related to subcellular partitioning and chemical forms of Cd and well-coordinated physiological responses.
研究领域:
真菌学
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环境毒理学
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植物生理学
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DOI
PubMed
高影响因子
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