ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 3.8
Xia Yong,Wang Qi,Wang Xu,Shan Liancong,Zhang Bo,Li Sheng,Guo Chang
The accurate and rapid detection of fluoroquinolone antibiotics (FQs) is crucial, yet their structural homology often leads to cross-reactivity that compromise accuracy. Herein, we designed a poly(3,4-ethylenedioxythiophene) doped with a poly(styrenesulfonate) (PEDOT:PSS)-based dual-channel organic electrochemical transistor (OECT) array on a flexible, low-cost polyimide substrate. By leveraging the synergistic catalysis of single-walled carbon nanotubes (SWCNTs) and gold nanoparticles (AuNPs), effective discrimination of three FQs was achieved using gate electrodes modified with only two Nafion/SWCNTs/AuNPs composites, differing solely in AuNP mass fraction—this significantly simplifies fabrication for an electronic tongue system (ETS). Aided by principal component analysis (PCA), the system discriminates and quantifies ofloxacin (OFL), levofloxacin (LEV), and ciprofloxacin (CIP) across a wide concentration range of 0.4–1000 μM, with limits of detection (LODs) of 0.32–0.40 μM, while exhibiting excellent selectivity, anti-interference, consistency, stability, and rapid response. The comprehensive performance of this platform establishes a practical foundation for portable FQ sensing in environmental and clinical applications. Graphical abstract
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Analytical Methods
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2026
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IF 2.6
Tianyi Zhu,Jian Zhang,Danyi He,Yiwen Yang,Hailong Wang,Jianbo Wang,Longhua Guo,Lei Li,Jianrong Chen,Yanbo Zeng
Oxytetracycline (OTC) residues have seriously threatened human health, as they may trigger allergic responses, hepatotoxicity, and the development of bacterial resistance. Detecting OTC in samples is therefore important. This work developed a pyrene-based fluorescent molecularly imprinted polymer (Py-FMIP) to rapidly and selectively detect OTC. N-allyl-4-(pyren-1-yl) butanamide (Al-Py), a new alkenyl functional monomer, was synthesized using 1-pyrenebutyric acid as the raw material with simple synthesis process. Py-FMIP was then obtained with Al-Py as a functional monomer and OTC as a template molecule. The Al-Py and Py-FMIP absolute fluorescence quantum yields were 74.05% and 69.27%, separately, suggesting the high fluorescence performances of two materials. The fluorescence of Py-FMIP was strongly quenched by OTC through inner filter effect. The prepared Py-FMIP sensor responded to OTC within 1 min, its linear detection range was 0.5–150 μM, and its detection limit was 0.05 μM (S/N = 3). The prepared Py-FMIP sensor also exhibited notable selectivity toward OTC. The recoveries of the Py-FMIP fluorescent sensor were 96.37%–104.60% in milk and tap water, with relative standard deviations lower than 5.13%.
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JOURNAL OF FLUORESCENCE
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2026
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IF 3.1
Zheng Xinxiang,Xu Hui,Zhang Runke,Zhao Tengzhi,Wang Zhaofeng,Zhao Xingfei,Wang Hua
In this study, a dual-ratiometric optical probe utilizing europium-doped carbon dots (Eu-CDs) was developed for simultaneous fluorescent and colorimetric detection of tetracycline (TC). The Eu-CDs complex was synthesized through a hydrothermal approach. Upon coordination with Eriochrome Black T (EBT), the Eu-CDs complex exhibits a distinct magenta coloration. Introduction of TC triggers a ligand displacement reaction, resulting in color transition from magenta to blue, resulting in ratiometric colorimetric visual detection. Meanwhile, the TC-bound EBT-CDs@Eu complex emits ratiometric fluorescence change. A ratiometric fluorescence detection method was established for TC based on the absorbance-energy transfer-emission (AETE) mechanism, culminating in the successful design of a dual-ratiometric optical probe. The colorimetric assay demonstrated a detection limit (LOD) of 20.5 nM for TC. Furthermore, the practical applicability of this sensor was validated through successful TC detection in aquaculture samples, highlighting its potential for real-world monitoring applications.
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Biosensors-Basel
·
2026
·Vol. 16
·期 7
·388
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IF 6.2
Yaning Liang,Shiyi Fang,Zhuoer Chen,Yuzhuo Chen,Qingqing Yang,Xuelan Shu,Tao Le
Gold nanoparticles (AuNPs) are widely used in aptasensors because of their high extinction coefficient and aggregation-dependent color differences. However, recent studies have indicated that nonspecific interactions between target molecules and AuNPs may dominate the detection signal rather than aptamer–target specific binding. This study systematically investigated the interactions between 13 aminoglycoside antibiotics and AuNPs. We found that all aminoglycoside antibiotics interacted strongly with AuNPs, considerably reducing their salt stability. Furthermore, methoxy polyethylene glycol thiol reversed AuNP aggregation induced by aminoglycoside antibiotics, indicating that it occurs at the secondary minimum. Using density functional theory, we analyzed the molecular structures and charge distribution characteristics of the aminoglycoside antibiotics, elucidating that they replace citrate ions on AuNP surfaces via a ligand exchange mechanism, thereby inducing aggregation. Additionally, both aptamer targets and complementary DNA struggled to desorb the aptamer (KAN6-1) from the AuNP surfaces. Our study demonstrates that the label-free colorimetric assay based on aggregation of unmodified citrate–AuNPs is neither suitable for characterizing the binding affinity of aminoglycoside aptamers nor viable for constructing corresponding colorimetric sensors to detect this class of antibiotics. Thus, researchers should incorporate mechanistic verification and rigorous controls when employing this system to ensure reliable results.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Gengchen Hu,Shizhao Liu,Yiming Zhang,Yao Jiang,Dongmei Xi
The widespread use of aminoglycoside antibiotics has raised serious concerns regarding food safety and environmental health, necessitating advanced methods for their sensitive and multiplexed detection. However, conventional techniques struggle to directly quantify these small molecules with sufficient sensitivity and specificity. Here, we present an ultrasensitive and simultaneous sensing platform for kanamycin (KAN) and tobramycin (TOB) that synergistically merges a target-triggered DNA walker with a biological α-hemolysin (α-HL) nanopore. In this design, aptamer recognition of the antibiotic activates a DNAzyme-based walker immobilized on magnetic beads, which then processively cleaves a track strand to release hundreds of DNA reporter probes per binding event. This dual-amplification strategy of catalytic chemical amplification coupled with single-molecule physical readout enables exceptional sensitivity, achieving detection limits as low as 3.88 fM for KAN and 4.62 fM for TOB. The sensing platform exhibits excellent selectivity and successfully accomplishes the simultaneous quantification of both antibiotics in a mixture. When applied to spiked environmental water samples, the method demonstrates high accuracy and reliability, yielding satisfactory recovery rates. This work establishes a generalizable DNA walker–nanopore paradigm, offering a versatile blueprint for the development of highly sensitive, multiplexed, and rapid detection systems for a wide range of small-molecule contaminants.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2025
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IF 11.3
Xi Li,Zhigang Yu,Jiaqing Ye,Xiaolong Wang,Huai Lin,Liusheng Lei,Wenjin Hu,Yi Luo
Numerous disinfectants have been discharged into the environment through anthropogenic activities, leading to ubiquitous coexistence of multiple disinfectants in the real environment. Previous studies reported that individual disinfectants induce the emergence of antimicrobial resistance (AMR); however, the impacts of the coexposure to multiple disinfectants on the development of AMR and its toxicity associated with bacterial pathogenicity are still unclear. Here, we conducted a 30-day stepwise exposure experiment to investigate the impacts of the commonly used disinfectants polyhexamethylene guanidine (PHMG), benzalkonium chloride (BAC), and their combination on AMR and pathogenicity of Wild-Type Escherichia coli. Results reveal that the coexistence of dual disinfectants dramatically led to higher levels of AMR (2.0- to 14.8-fold) and pathogenicity (8.7-fold) in the evolved strains. Dual disinfectant-induced bacterial evolution is primarily driven by the alteration in the expression of key functional genes, such as those involved in biofilm formation and cell motility. In contrast, genetic mutations may not account for the increased AMR and pathogenicity in combination of dual disinfectant treatment, as similar gene mutations are responsible for both dual and single disinfectant stress. The evolved strains induced by disinfectant combination, characterized with transcriptional regulation, exhibited a synergistic or additive response on AMR and pathogenicity, which was further confirmed by a toxicology prediction model. This study demonstrated that dual disinfectants synergistically enhanced AMR and the associated pathogenicity, advancing our understanding of the risks posed by coexistence of multiple chemical pollutants in the environment.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2025
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IF 3
Nie Saiyu,Zhang Peng,Zhang Qian,Ding Caifeng
The contamination of marine environments with antibiotics, such as kanamycin, poses a significant ecological threat due to its potential harmful effects on marine organisms. Conventional methods for detecting kanamycin, including high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assays (ELISA), face challenges in terms of cost, complexity, and long detection times. This research aims to address these limitations by developing a novel, highly sensitive biosensing platform for kanamycin detection based on a DNAzyme-driven cascade reaction coupled with nanoparticle technology. The method utilizes aptamers for specific kanamycin recognition, which activates DNAzyme-1, triggering a downstream DNA walker system. This cascade reaction amplifies fluorescence signals, significantly enhancing sensitivity. The optimized platform demonstrated a wide detection range (0.05–3 nmol/L) and a low detection limit of 0.0327 nmol/L. This system showed excellent specificity for kanamycin, even in the presence of other antibiotics, and was successfully applied to detecting kanamycin in seawater samples with high recovery rates (94%–102.9%). This DNAzyme-based biosensor offers a promising approach for rapid, cost-effective, and high-sensitivity monitoring of antibiotic contamination in aquatic environments, with potential applications in environmental monitoring and ecological protection.
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MICROCHIMICA ACTA
·
2022
·Vol. 190
·期 1
·1-9
·
IF 6.408
Fan Yunfeng,Liu Zhimin,Wang Jie,Cui Chen,Hu Leqian
A novel electrochemiluminescence (ECL) aptasensor for the determination of lincomycin (LIN) was developed based on CdS QDs/carboxylated g-C 3 N 4 (CdS QDs/C-g-C 3 N 4 ). CdS QDs/C-g-C 3 N 4 served as the substrate of the aptasensor, and then CdS QDs/C-g-C 3 N 4 -modified electrode was incubated with aptamer DNA (Apt-DNA). When the non-specific sites of the electrode surface was blocked by 6-mercaptohexanol, the ferrocene-labeled probe (Fer-DNA) was assembled onto the electrode surface through base complementation with Apt-DNA. In the absence of LIN, the ECL signal was quenched effectively by Fer-DNA and a decreased ECL emission (off state) was acquired. On the contrary, LIN was specifically bond with Apt-DNA, and Fer-DNA was detached from the aptasensor surface because of the deformation of Apt-DNA, resulting in an effectively enhanced ECL signal (on state). The constructed ECL aptasensor exhibited a wide detection range for LIN determination (0.05 ng mL −1 –100 μg mL −1 ) with a low detection limit (0.02 ng mL −1 ). Importantly, the proposed ECL aptasensor showed outstanding accuracy and specificity for LIN determination, and also provided a potential strategy for other antibiotic determinations. Graphical Abstract
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PEST MANAGEMENT SCIENCE
·
2022
·Vol. 79
·期 1
·173-182
·
IF 4.462
Dongbiao Lü,Yanlu Dong,Zizheng Yan,Xueying Liu,Yongjun Zhang,Daibin Yang,Kanglai He,Zhenying Wang,Ping Wang,Xiangqun Yuan,Yiping Li
BACKGROUND Spodoptera frugiperda is an important invasive agricultural pest that causes huge economic losses worldwide. Gut microorganisms play a vital role in host feeding, digestion, nutrition, immunity, growth and insecticide resistance. Illumina high-throughput sequencing was used to study the gut microbial community dynamics across the life cycle (egg, 1st to 6th instar larvae, pupae, and male and female adults) of S. frugiperda fed on maize leaves. Furthermore, the gut microbial community and food intake of the 5th instar S. frugiperda larvae were studied after feeding them antibiotics. RESULTS Enterobacteriaceae and Enterococcaceae dominated the gut during growth and feeding of the larvae. The relative abundance of Enterobacteriaceae was higher in the 4th and 6th instar larvae. With the increase in larval feeding, the relative abundance of Enterococcaceae gradually increased. In addition, principal coordinate analysis and linear discriminant effect size analysis confirmed differences in the structure of gut microbiota at different developmental stages. After antibiotic treatment, the relative abundance of Firmicutes, Proteobacteria and Fusobacteriota decreased. The relative abundance of Enterococcus and Klebsiella decreased significantly. Antibiotic treatment inhibited the gut flora of S. frugiperda , which decreased larval food intake and body weight gain, and prolonged the larval stage. CONCLUSION The composition of the gut bacterial community plays an important role in the growth, development, and feeding of S. frugiperda . The results have a certain theoretical value for the development of bio-pesticides targeting intestinal flora. © 2022 Society of Chemical Industry.
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ACS Nano
·
2023
·
IF 17.1
Hai Liu,Wei Huang,Yang Yu,Da Chen
Conventional oxidative disinfection methods are usually inefficient to eliminate intracellular antibiotic resistance genes (i-ARGs) due to competitive oxidation of cellular components of antibiotic-resistant bacteria (ARB), resulting in the ubiquitous occurrence of ARGs in drinking water systems. Herein, we developed the strategy of coupling electroporation and electrochemical oxidation on a Co3O4-nanowires-modified electrode to destroy the multiresistant Escherichia coli cells and promote subsequent i-ARG (blaTEM-1 and aac(3)-II) degradation. The lightning-rod effect over nanowire tips can form finite regions with a locally enhanced electric field and highly concentrated charge density, in turn facilitating the electroporation for ARB cell damage and electrochemical reactivity for reactive chlorine/oxygen species generation. Characterization of the ARB membrane integrity and morphology revealed that electroporation-induced cell pores were further enlarged by the oxidation of reactive species, resulting in i-ARG removal at lower applied voltages and with 6–9 times lower energy consumption than the conventional electrochemical oxidation approach with a Co3O4-film-modified electrode. The satisfactory application and effective inhibition of horizontal gene transfer in tap water further demonstrated the great potential of our strategy in the control of the ARG dissemination risk in drinking water systems.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Tingting Liu,Xiaocui Lai,Ping Guo,Wei Zhang,Gan Zhang,Mengyun Wu,Guangjian Xue,Xuechen Fang,Juan Peng,Weihua Lai
As kanamycin (KAN) residue in animal products is harmful to consumers, a rapid and sensitive method for KAN detection needs to be established. KAN monoclonal antibody (KAN-mAb, 1D11) with the half maximal inhibitory concentration of 1.16 ng/mL was prepared in this study. A one-pot method was used to synthesize Fe 3+ -chelated polydopamine nanospheres (Fe@PDANs) with excellent characteristics of strong light absorption. The novel label of Fe@PDANs and KAN-mAb was used to develop a lateral flow immunoassay (LFIA) for the sensitive detection of KAN. The limit of detection of the Fe@PDANs-based LFIA (Fe@PDANs-LFIA) for KAN was 0.0191 ng/mL, which was 2.75 times lower than PDANs-based LFIA. Furthermore, the Fe@PDANs-LFIA was successfully applied to detect KAN in pork, milk, and honey samples, with recoveries ranging from 93.75% to 113.80% (coefficient of variation < 10%). Therefore, Fe@PDANs have potential for the detection of analytes in LFIA.
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ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Fengzhe Li,Yingying Chen,Jinhua Shang,Qing Wang,Shizhen He,Xiwen Xing,Fuan Wang
Abnormal DNA methylation contributes to the annoying tumorigenesis and the elevated expression of methylation-related methyltransferase (MTase) is associated with many diseases. Hence DNA MTase could serve as a promising biomarker for cancer-specific diagnosis as well as a potential therapeutic target. Herein, we developed an isothermal autocatalytic hybridization reaction (AHR) circuit for the sensitive detection of MTase and its inhibitors by integrating the catalytic hairpin assembly (CHA) converter with the hybridization chain reaction (HCR) amplifier. The initiator-mediated HCR amplifier could generate amplified fluorescent readout, as well as numerous newly activated triggers for motivating the CHA converter. The CHA converter is designed to expose the identical sequence of HCR initiators that reversely powered the HCR amplifier. Thus, the trace amount of target could produce exponentially amplified fluorescent readout by the autocatalytic feedback cycle between HCR and CHA systems. Then an auxiliary hairpin was introduced to mediate the assay of Dam MTase via the well-established AHR circuit. The Dam MTase-catalyzed methylation of auxiliary hairpin leads to its subsequent efficient cleavage by DpnI endonuclease, thus resulting in the release of HCR initiators to initiate the AHR circuit. The programmable nature of the auxiliary hairpin allows its easy adaption into other MTase assay by simply changing the recognition site. This proposed AHR circuit permits a sensitive, robust, and versatile analysis of MTase with the limit of detection (LOD) of 0.011 U/mL. Lastly, the AHR circuit could be utilized for MTase analysis in real complex samples and for evaluating the cell-cycle-dependent expression of MTase. This developed MTase-sensing strategy holds promising potential for biomedical analysis and clinical diagnosis.
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