PLoS One
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2015
·Vol. 10
·期 3
·e0116832
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IF 3.234
Yan-Ling Zheng,Li-Ping Zhang,Xue-Liang Zhang,Kai Wang,Yu-Jian Zheng
Tuberculosis is a major global public health problem, which also affects economic and social development. China has the second largest burden of tuberculosis in the world. The tuberculosis morbidity in Xinjiang is much higher than the national situation; therefore, there is an urgent need for monitoring and predicting tuberculosis morbidity so as to make the control of tuberculosis more effective. Recently, the Box-Jenkins approach, specifically the autoregressive integrated moving average (ARIMA) model, is typically applied to predict the morbidity of infectious diseases; it can take into account changing trends, periodic changes, and random disturbances in time series. Autoregressive conditional heteroscedasticity (ARCH) models are the prevalent tools used to deal with time series heteroscedasticity. In this study, based on the data of the tuberculosis morbidity from January 2004 to June 2014 in Xinjiang, we establish the single ARIMA (1, 1, 2) (1, 1, 1) 12 model and the combined ARIMA (1, 1, 2) (1, 1, 1) 12 -ARCH (1) model, which can be used to predict the tuberculosis morbidity successfully in Xinjiang. Comparative analyses show that the combined model is more effective. To the best of our knowledge, this is the first study to establish the ARIMA model and ARIMA-ARCH model for prediction and monitoring the monthly morbidity of tuberculosis in Xinjiang. Based on the results of this study, the ARIMA (1, 1, 2) (1, 1, 1) 12 -ARCH (1) model is suggested to give tuberculosis surveillance by providing estimates on tuberculosis morbidity trends in Xinjiang, China.
研究领域:
流行病学
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传染病学
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生物统计学
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公共卫生
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预防医学
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DOI
高影响因子
Acta Cardiologica Sinica
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2018
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IF 0.927
Li-Tan Yang,Yao-Mao Chang,Tsung-Han Hsieh,Wen-Hsuan Hou,Chung-Yi Li
Background We conducted a time-series analysis of daily ambient temperature and all-cause, cardiovascular, and respiratory disease mortality in Taiwan, which is generally neither extremely hot nor cold. Methods Data on all-cause daily mortality rates (excluding accidents, suicide, and homicide), and mortality rates due to respiratory and cardiovascular diseases between 2008 and 2010 were obtained from the Taiwan Death Registry. The daily temperature for that period was averaged from 33 monitoring stations nationwide. A generalized least square model was constructed to assess the relationship between the time-series trends of temperature and mortality, and the cross-correlation function was used to determine the possible time lag for the effect of temperature on mortality. Results As the average temperature increased, the daily all-cause (β = -0.006) and respiratory disease (β = -0.012) mortality rates decreased. On the other hand, an inverse relationship (β = -0.028) between average daily temperature and cardiovascular disease mortality was observed only for a temperature between 12.91 °C and 26.36 °C. The time lag for all-cause and cardiovascular disease mortality was similar at 4-6 days, while the lag for respiratory disease was longer at 13-16 days. Conclusions We found inverse associations between average temperature and all-cause and respiratory mortality. An inverse association between temperature and cardiovascular disease mortality was observed only from 12.91 °C to 26.36 °C.
研究领域:
流行病学
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环境医学
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公共卫生
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心血管病学
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呼吸病学
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DOI
PubMed
高影响因子
Biomed Research International
·
2014
·
IF 0
Jianhua Hao,Junzhong Liu,Mi Sun
A bacterial strain C5 that can produce new type of marine esterase was isolated and screened from marine sludge. According to 16S rRNA sequence analysis and physiological and biochemical experiments, the strain was identified as Bacillus subtilis. A single isozyme with a molecular weight of 86 kDa was observed by SDS-PAGE and native-PAGE. On this basis, the mechanism of esterase B1 secreted by strain C5 degrading parathion-methyl was explored, and the effects of temperature and pH on the degradation rate were investigated. From the results, p-nitrophenol was one of the degradation products of B1 degrading parathion-methyl, and the best degradation effect could be achieved at the temperature of 40°C and the neutral pH value.
研究领域:
微生物学
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环境科学
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生物技术
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产品关联
DOI
PubMed
Foods
·
2024
·Vol. 13
·期 11
·1777
·
IF 4.7
Xianwen Ma,Dian Zou,Anying Ji,Cong Jiang,Ziyue Zhao,Xiaoqi Ding,Zongchen Han,Pengfei Bao,Kang Chen,Aimin Ma,Xuetuan Wei
In this study, a novel strain for degrading chitin was identified asBacillus paralicheniformisHL37, and the key chitinase CH1 was firstly mined through recombinant expression inBacillus amyloliquefaciensHZ12. Subsequently, the sequence composition and catalytic mechanism of CH1 protein were analyzed. The molecular docking indicated that the triplet of Asp526, Asp528, and Glu530 was a catalytic active center. The enzymatic properties analysis revealed that the optimal reaction temperature and pH was 65 °C and 6.0, respectively. Especially, the chitinase activity showed no significant change below 55 °C and it could maintain over 60% activity after exposure to 85 °C for 30 min. Moreover, the optimal host strain and signal peptide were obtained to enhance the expression of chitinase CH1 significantly. As far as we know, it was the first time finding the highly efficient chitin-degrading enzymes inB. paralicheniformis, and detailed explanations were provided on the catalytic mechanism and enzymatic properties on CH1.
研究领域:
微生物学
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Xusen Cheng,Bo Liu,Yunfeng Qiu,Ke Liu,Zhuluni Fang,Jinteng Qi,Zhuo Ma,Tiedong Sun,Shaoqin Liu
Commonly used dense arrays of nanomaterials on carbon cloth (CC) are not suitable to accommodate microorganisms in microbial fuel cells (MFCs) due to their unmatched size. To simultaneously enrich exoelectrogens and accelerate the extracellular electron transfer (EET) process, SnS 2 nanosheets were selected as sacrificial templates to prepare binder-free N,S-codoped carbon microflowers (N, [email protected] ) by polymer coating and pyrolysis . N, [email protected] showed a cumulative total charge of 125.70C/m 2 , approximately 2.11 times higher than that of CC, indicating its better electricity storage capacity. Moreover, the interface transfer resistance and diffusion coefficient in bioanodes were 42.68 Ω and 9.27 × 10 -10 cm 2 /s, respectively, superior to CC (141.3 Ω and 1.06 × 10 -11 cm 2 /s). Remarkably, N,S-codoped carbon microflowers excreted more flavin than CC, as confirmed by continuous fluorescence monitoring. Biofilm and 16S rRNA gene sequence analysis revealed that exoelectrogens were enriched, and nanoconduits were generated on the N, [email protected] anode. In particular, flavin excretion was also promoted on our hierarchical electrode, effectively driving the EET process. MFCs equipped with the N, [email protected] anode could deliver a power density of 2.50 W/m 2 , coulombic efficiency of 22.77 %, and chemical oxygen demand (COD) removal amount of 90.72 mg/L/d, higher than that of bare CC. These findings not only demonstrate that our anode is capable of solving the cell enrichment issue, but it may also increase EET rates by bound flavin with outer membrane c-type cytochromes (OMCs) to simultaneously boost the power generation and wastewater treatment performance of MFCs.
研究领域:
环境科学
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材料科学
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微生物学
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电化学
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产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2025
·
IF 4
Xiao-Ling Tang,Kang Zhao,Teng-Kun Huang,Bai-Hua He,Xu-Gang Zhang,Zhi-Qiang Liu,Yu-Guo Zheng
O -Succinyl- L -homoserine mercaptotransferase (MetZ) is a key enzyme for the synthesis of L -methionine. However, the limited tolerance to high concentrations of substrate methyl mercaptan sodium restricts its industrial applications, while the “trade-off” between activity and stability has always been a challenge for protein engineering. To address this problem, a strategy of dynamic network rigidification-local flexibility regulation was applied in this study to engineer the enzyme from Chromobacterium violaceum ( Cv MetZ). Through consensus sequence analysis and structure-guided design, the hotspot amino acid residues were anchored for mutagenesis. The best mutant T59I/T119Y/A241T was obtained with synergistic enhancement of activity and stability. The half-life ( t 1/2 ) of whole cells harboring the mutant was increased from 3.3 and 1.3 h to 20.6 and 7.2 h, in the presence of 5% and 10% methyl mercaptan sodium, respectively. The engineered enzyme was successfully immobilized with ideal usability, which laid the foundation for efficient and scale-up bioproduction of L -methionine.
研究领域:
酶工程
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蛋白质设计
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结构生物学
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工业生物技术
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生物催化
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代谢工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）