Parasites & Vectors
·
2015
·Vol. 8
·期 1
·1-8
·
IF 3.43
Yu Bao-Ting,Ding Yan-Mei,Mo Jian-Chu
Background Most mosquito species need to obtain sugar from host plants. Little is known about the chemical cues that Culex pipiens pallens use during their orientation to nectar host plants. In this study, we investigated the behavioural responses of female Cx. pipiens pallens to common floral scent compounds and their blends.Methods Behavioural responses of female Cx. pipiens pallens to 18 individual compounds at different concentrations were determined in the olfactometer bioassays. A synthetic blend composed of behaviourally active compounds was formulated, and its attractiveness to mosquitoes was tested. Several most attractive compounds constituted a reduced blend, and its attractiveness was tested against the solvent and the full blend, respectively. Mosquito response in the olfactometer was analyzed by comparing the percentages of mosquitoes caught in the two arms by χ2 test (observed versus expected).Results Fifteen of the 18 compounds were attractive to female Cx. pipiens pallens in the dose-dependent bioassays, with the exception of β-pinene, acetophenone and nonanal. (68.00 ± 2.49) % mosquitoes responded to the full blend composed of these 15 compounds on their optimal doses when tested against the solvent, with the preference index at 46.11 ± 3.57. Six individual compounds whose preference indices were over 40 constituted the reduced blend, and it attracted (68.00 ± 1.33) % mosquitoes when tested against the solvent while its preference index was 42.00 ± 3.54. When tested against the full blend simultaneously in the olfactometer, the reduced blend could attract (45.00 ± 2.69) % of released mosquitoes, which was as attractive as the full blend.Conclusion sOur results demonstrate that female Cx. pipiens pallens is differentially attracted by a variety of compounds at different concentrations. Alteration of the concentration strongly affects the attractiveness of the synthetic blend. Several floral scent volatiles might be the universal olfactory cues for various mosquito species to locate their nectar host plants, which could be potentially used in trapping devices for surveillance and control of them.
研究领域:
昆虫学
·
化学生态学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2017
·Vol. 22
·期 2
·205
·
IF 2.861
Mei Ping Zhao,Qi Zhi Liu,Qiyong Liu,Zhi Long Liu
The screening of Chinese medicinal herbs for insecticidal principles showed that the essential oil ofEchinops grijsiiHance roots possessed significant larvicidal activity against mosquitoes. The essential oil was extracted via hydrodistillation and its constituents were determined by gas chromatography-mass spectrometry (GC-MS) analysis. GC-MS analyses revealed the presence of 31 components, with 5-(3-buten-1-yn-1-yl)-2,2′-bithiophene (5-BBT, 27.63%), α-terthienyl (α-T, 14.95%), 1,8-cineole (5.56%) andcis-β-ocimene (5.01%) being the four major constituents. Based bioactivity-directed chromatographic separation of the essential oil led to the isolation of 5-BBT, 5-(4-isovaleroyloxybut-1-ynyl)-2,2′-bithiophene (5-IBT) and α-T as active compounds. The essential oil ofE. grijsiiexhibited larvicidal activity against the fourth instar larvae ofAedes albopictus,Anopheles sinensisandCulex pipiens pallenswith LC50values of 2.65 μg/mL, 3.43 μg/mL and 1.47 μg/mL, respectively. The isolated thiophenes, 5-BBT and 5-IBT, possessed strong larvicidal activity against the fourth instar larvae ofAe. albopictus(LC50= 0.34 μg/mL and 0.45 μg/mL, respectively) andAn. sinensis(LC50= 1.36 μg/mL and 5.36 μg/mL, respectively). The two isolated thiophenes also had LC50values against the fourth instar larvae ofC. pipiens pallensof 0.12 μg/mL and 0.33 μg/mL, respectively. The findings indicated that the essential oil ofE. grijsiiroots and the isolated thiophenes have an excellent potential for use in the control ofAe. albopictus,An. sinensisandC. pipiens pallenslarvae and could be used in the search for new, safer and more effective natural compounds as larvicides.Keywords:Echinops grijsii;Aedes albopictus;Anopheles sinensis;Culex pipiens pallens;larvicidal activity
研究领域:
昆虫学
·
植物化学
·
公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION
·
2014
·Vol. 30
·期 3
·199-203
·
IF 0.827
Wei-Bin Ma,Jun-Tao Feng,Zhi-Li Jiang,Xing Zhang
We studied the knockdown activity and lethal toxicity of 6 essential oil compounds—methyl salicylate, linalool, 2-phenethyl alcohol, eugenol, β-citronellol, and trans-cinnamaldehyde—as fumigants against adult female Culex pipiens pallens in the laboratory. Of the 6 products tested, trans-cinnamaldehyde was the most toxic (LC50 = 0.26 µl/l air, 24 h) with a slow knockdown time (KT95 = 176.5 min at 0.5 µl/l air). Methyl salicylate displayed a lower toxicity (LC50 = 1.17 µl/l air, 24 h) but the fastest knockdown activity (KT95 = 16.8 min) at the sublethal concentration 0.5 µl/l air. Furthermore, the binary mixture of methyl salicylate and trans-cinnamaldehyde exhibited a combined effect of fast knockdown activity and high toxicity against Cx. p. pallens adults, showing potential for development as natural fumigants for mosquito control.
研究领域:
昆虫学
·
公共卫生学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MEDICAL ENTOMOLOGY
·
2018
·Vol. 55
·期 4
·787-794
·
IF 1.968
Tian Jiaxin,Mao Jie,Yu Baoting,Fouad Hatem,Ga’al Hassan,Mao Guofeng,Mo Jianchu
Efforts to develop mosquito attractants using vertebrate host volatiles have been well made under laboratory conditions but their attractiveness to mosquitoes in the wild still needs to be evaluated. In the present study, we evaluated the attraction of female Culex pipiens pallens Coquillett (Diptera: Culicidae) to 11 individual chemical compounds found in vertebrate host odors, and to synthetic blends, consisting of different combinations of the compounds. These tests were conducted under laboratory and field conditions using a Y-tube olfactometer and odor-baited traps, respectively. When delivered at concentrations ranging from 0.1 to 10.0 μg/kg, 9 of the 11 compounds were attractive to female mosquitoes under laboratory conditions. We developed 47 synthetic blends composed of the 6 most attractive compounds (propionic acid, hexanal, myristic acid, benzaldehyde, 1-octen-3-ol, and geranyl acetone) and 18 of them were significantly attractive to mosquitoes in the olfactometer. Most of the attractive blends contained two to four attractive compounds. In the field, 5 of the 18 blends captured significantly more mosquitoes than did control traps. The findings demonstrate that female mosquitoes can be attracted by single chemical compounds as well as some of their synthetic blends. The effectiveness of synthetic blends depended on specific combinations of several compounds, rather than simply increasing the number of attractive compounds in the blends. Synthetic blends may have potential for use in odor-baited traps for mosquito surveillance.
研究领域:
昆虫学
·
公共卫生
·
病原生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2019
·
IF 3.926
Ahmed H. Tantawy,Shaimaa M. Farag,Lamees Hegazy,Hong Jiang,Man-Qun Wang
A series of piperine-based dienehydrazide derivatives were designed and synthesized to be used as insecticides against Culex pipiens . The chemical structure of compound 5n was confirmed by single-crystal x-ray diffraction. Their insecticidal activities of synthesized compounds were tested against third-instar larval of Cx. pipiens at concentrations ranging from 0.1 to 1.2 mg/mL. Among all derivatives, compounds 5a , 5b , 5f , 5g , 5m , 5n , 5o , 5p , and 5u displayed good activities. The final mortality rates at the concentration of 0.75 mg/mL after 48 h treatment, were found to be in the range from 80.00 to 83.33% and with LC 50 values ranging from 0.221 to 0.094 mg/mL. These compounds demonstrated higher insecticidal activities than piperine and Deltamethrin (a commercial positive control). Molecular modelling reveals several molecular interactions between synthesized compounds and the substrate binding sits of acetylcholinesterase (AChE) that are predicted to be responsible for its binding and inhibition activity. .
研究领域:
药理学
·
有机化学
·
昆虫学
✓ 已验证
DOI
高影响因子
ACS Agricultural Science & Technology
·
2022
·
IF 0
Yanke Hao,Hongfu He,Pan Zhou,Kaikai Niu,Hongjian Song,Yuxiu Liu,Jingjing Zhang,Deyu Hu,Qingmin Wang,Baoan Song
Aldisine alkaloid has a wide range of biological activities and is also the core skeleton of a variety of natural products, but its poor solubility affects its efficacy. In order to improve its solubility and biological activity, a series of indoloazepinone derivatives containing oxime ether, oxime ester, hydrazone, and ester moieties were designed and synthesized, and their antiviral, fungicidal, and larvicidal activities were evaluated. The solubility of these indoloazepinone derivatives improved in comparison with that of aldisine, and most of these compounds showed good antiviral activities against pepper mild mottle virus (PMMoV), among which the 4-chlorobenzoyl oxime ester derivative 5i showed the highest protection, curative, and inactivation activities in vivo (inhibition rate 61 ± 4.1, 60 ± 5.3, and 74 ± 4.2% at 500 mg/L, respectively), which was comparable to that of ningnanmycin (59 ± 2.2, 58 ± 0.7, and 81 ± 4.8% at 500 mg/L, respectively). Compound 5i (the rat acute oral toxicity test, LD50 > 2000 mg/kg) emerged as a promising candidate for anti-PMMoV drug. Most of these compounds have broad-spectrum fungicidal activities, and 5a and 5f showed selectively fungicidal activities against Phytophthora capsici. In addition, these compounds also showed good larvicidal activities against Plutella xylostella and Culex pipiens pallens.
研究领域:
药理学
·
化学
·
植物病理学
·
昆虫学
·
病毒学
✓ 已验证
DOI
危险品化学品经营许可证（带存储）