ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 11
·2529-2536
·
IF 3.637
Zhang Baozhu,Wei Chunying
A simple turn-on fluorescence strategy is proposed for the detection of ATP based on DNA-stabilized copper/silver nanoclusters (DNA-Cu/Ag NCs). The fluorescence intensity of DNA-Cu/Ag NCs increases significantly in the presence of ATP, because the specific interaction between ATP and its aptamer causes two darkish Cu/Ag NCs to be situated at the 5′ and 3′ termini close to each other. A limit of detection (LOD) of 7.0 μM is found, in a linear range of 2–18 mM, and the proposed sensor is simple, sensitive, and selective. Additionally, the DNA-Cu/Ag NCs/ATP system is further developed into a sensor for ADA detection and demonstrates a linear response to ADA from 5 to 50 U/L with a LOD of 5 U/L. The proposed method is also shown to be successful in detecting ATP and ADA in a solution of fetal bovine serum.
研究领域:
分析化学
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纳米技术
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生物传感器
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DOI
PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 10
·832
·
IF 5.743
Yuliang Cheng,Guowen Li,Xiufang Huang,Zhijuan Qian,Chifang Peng
In this work, a label-free fluorescent detection method for glyphosate, based on DNA-templated silver nanoclusters (DNA-Ag NCs) and a Cu2+-ion-modulated strategy, was developed. In the presence of Cu2+, the fluorescence of the DNA-Ag NCs was quenched. Glyphosate can restore the fluorescence of DNA-Ag NCs. By analyzing the storage stability of the obtained DNA-Ag NCs using different DNA templates, specific DNA-Ag NCs were selected for the construction of the glyphosate sensor. The ultrasensitive detection of glyphosate was achieved by optimizing the buffer pH and Cu2+concentration. The sensing of glyphosate demonstrated a linear response in the range of 1.0–50 ng/mL. The limit of detection (LOD) was 0.2 ng/mL. The proposed method was successfully applied in the detection of glyphosate in a real sample, indicating its high application potential for glyphosate detection.Keywords:label free;fluorescence;turn on;DNA;Cu2+;silver nanoclusters
研究领域:
分析化学
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环境科学
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纳米技术
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生物技术
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DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 12
·期 2
·188-192
·
IF 2.378
Yanling Huang,Guiyin Yue,Fang Luo,Longhua Guo,Bin Qiu,Zhenyu Lin
Hydrogen sulfide (H2S) is a dramatically toxic gas molecule; long term exposure to H2S environment poses a serious threat to human health. It is highly imperative to develop sensitive and selective methods for H2S detection. In this study, a signal-on fluorescence biosensor for the H2S (used to represent the total amount of H2S, HS− and S2−) detection was developed based on silver-mediated base pairs (C–Ag+–C). Two separate C-rich single-stranded DNAs (ssDNAs) were synthesized, namely, one was labeled with fluorescein (6-carboxyfluorescein, FAM) and the other was labeled with quencher (black hole quencher, BHQ1). The two ssDNAs were in a flexible single strand owing to the mismatch of the ssDNA, and hence, significant fluorescence intensity was observed. In the presence of Ag+, DNA probes could selectively capture Ag+ to form double stranded DNA (dsDNA) via the C–Ag+–C base pairs, which made the quench of FAM by BHQ1 and low fluorescence signal was detected. With the addition of H2S to the system, the dsDNA was decomposed into ssDNA since Ag+ could be released from C–Ag+–C by the formation of a more stable complex Ag2S, which resulted in the recovery of the fluorescence intensity. The enhanced fluorescence intensity had a linear relationship with H2S concentration in the range from 0.5 to 20 μM with a detection limit (LOD) of 0.16 μM (S/N = 3). The proposed system was applied to detect H2S (used to represent the total amount of H2S, HS− and S2−) in air near a garbage station with satisfying results.
研究领域:
环境科学
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分析化学
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生物化学
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产品关联
DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 8
·5594-5599
·
IF 3.9
Yuxia Li,Zeting Meng,Yating Liu,Baozhu Zhang
A turn-on fluorescence nanoprobe was constructed for the determination of adenosine 5′-triphosphate (ATP) based on DNA-templated silver nanoclusters (DNA-AgNCs). The significant enhancement fluorescence intensity of DNA-AgNCs in the presence of ATP is due to the high special binding affinity between ATP and the aptamer, resulting in the environment of DNA-AgNCs with darkish fluorescence lying at one terminus of DNA slightly altering owing to the change of ATP aptamer conformation. A good linear range runs from 9 to 24 mM with a satisfactory detection limit of 3 μM. Furthermore, the proposed nanoprobe exhibited good performance for ATP detection in diluted fetal bovine serum.
研究领域:
分析化学
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纳米技术
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生物化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2021
·
IF 6.057
Hong Huang,Biyun Chen,Lifen Li,Yuan Wang,Zhangfeng Shen,Yangang Wang,Xi Li
Endoplasmic reticulum (ER) is an indispensable organelle responsible for protein synthesis , transportation, and maintenance of Ca 2+ homeostasis in eukaryotic cells. Recent studies highlighted that ER-targeted photosensitizers with high yield of singlet oxygen ( 1 O 2 ) are effective in selectively disrupting ER function and are promising candidates for anticancer therapy. Unfortunately, no ER targetable fluorescent probes for determining 1 O 2 photosensitized in this photodynamic therapy process is available. In this work, we synthesized an ER-targetable, two-photon fluorescence probe, ER- 1 O 2 , for fluorescence turn-on sensing of 1 O 2 . ER- 1 O 2 demonstrated high sensitivity to 1 O 2 sensing with a wide detection range (0–2.75 μM) and a low detection limit (0.11 μM). ER- 1 O 2 also displayed excellent selectivity toward 1 O 2 out of other ROS and metal ions . Notably, ER- 1 O 2 exhibited low cytotoxicity but with specific ER targetable capability. On account of these advantageous features, fluctuations of 1 O 2 in living cells and brain tissues were effectively visualized by ER- 1 O 2 .
研究领域:
生物化学
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分子生物学
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癌症治疗
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神经科学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 5
·1-9
·
IF 5.705
Huang Jianyong,Li Fenglan,Guo Rubin,Chen Yuyuan,Wang Zhenzhen,Zhao Chengfei,Zheng Yanjie,Weng Shaohuang,Lin Xinhua
Amino-modified carbon dots (C-dots) with positively charged surface were prepared. They display strong blue fluorescence and are shown to act as quenchers of the green fluorescence of FAM-labeled ssDNA such as the F-probe used in this work that was immobilized on the C-dots. On the addition of highly negatively charged heparin (Hep), it will interact with the C-dots and displace the F-probe from C-dots. Once the F-probe is displaced by Hep, its green fluorescence is restored. The intrinsic blue fluorescence of the C-dots remains stable after addition of Hep. Thus, a signal-on ratiometric fluorometric assay was developed for the ultra-sensitive detection of Hep. The underlying mechanisms of quenching and recovery are discussed. Under optimized conditions, the recovery of the ratiometric fluorescence of the system composed of C-dots and quenched F-probe is proportional to the Hep concentration in the range of 0.01–2.0 μg·mL −1 (= 0.00125–0.25 U·mL −1 ). The method was successfully applied to the determination of Hep in spiked serum samples.
Schematic of a signal-on ratiometric fluorometric method for the ultra-sensitive detection of heparin on the basis of the displacement and fluorescence enhancement of adsorbed FAM-labeled ssDNA from amino-modified carbon dots (C-dots) by heparin.
研究领域:
生物化学
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分析化学
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医学检验
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2022
·Vol. 27
·期 1
·327
·
IF 4.927
Hang Xiao,Tao Li,Xiao-Li Sun,Wen-Ming Wan,Hongli Bao,Qingrong Qian,Qinghua Chen
Pyrene molecules containing NBN-doped polycyclic aromatic hydrocarbons (PAHs) have been synthesized by a simple and efficient intermolecular dehydration reaction between 1-pyrenylboronic acid and aromatic diamine. Pyrene-B (o-phenylenediamine) with a five-membered NBN ring and pyrene-B (1,8-diaminonaphthalene) with a six-membered NBN ring show differing luminescence. Pyrene-B (o-phenylenediamine) shows concentration-dependent luminescence and enhanced emission after grinding at solid state. Pyrene-B (1,8-diaminonaphthalene) exhibits a turn-on type luminescence upon fluoride ion addition at lower concentration, as well as concentration-dependent stability. Further potential applications of Pyrene-B (o-phenylenediamine) on artificial light-harvesting film were demonstrated by using commercial NiR dye as acceptor.Keywords:NBN-doped polycyclic aromatic hydrocarbon;grinding enhanced emission;fluoride ion detection;turn-on type luminescent;artificial light-harvesting film
研究领域:
有机化学
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 2
·1-8
·
IF 5.7
Li Shenghua,Xiao Lingyu,Xiao Longqian,Tan Hongliang
The potential of coordination polymers (CPs) as a host of integrating multiple guest species to construct a fluorescence resonance energy transfer (FRET) nanoprobe was demonstrated. The ZnCPs built from zinc(II) and adenine was employed as a model of CPs to integrate carbon dot (CD) and phenol red (PR) for producing the FRET nanoprobe (CD/PR@ZnCPs). Benefiting from the confinement effect of ZnCPs, the integrated CD and PR can be brought in close proximity to favor the occurrence of FRET process from CD to PR, which leads to the quenching of CD fluorescence. However, the FRET process was disrupted upon the red-shift of PR absorption from 428 to 562 nm in alkaline medium, and consequently switches on the fluorescence of CD/PR@ZnCPs. Based on this finding, by utilizing urease to hydrolyze urea and mediate medium pH, a turn-on fluorescent method was established for the detection of urease activity. This fluorescent method has a linear response that covers 5 to 150 U/L urease with a detection limit of 0.74 U/L and exhibits an excellent selectivity over other enzymes. The successful determination of urease in saliva samples demonstrates the applicability of the fluorescent nanoprobe in complex biological matrix. Graphical Abstract
研究领域:
分析化学
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生物传感器
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纳米技术
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酶学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 11
·1-9
·
IF 6.408
Luo Jiarong,Luo Junjun,Deng Lei,Yang Minghui,Chen Xiang
A “signal-on” photoelectrochemical (PEC) immunosensor was successfully constructed for determination of human epidermal growth factor receptor 2 (HER2) based on organic–inorganic heterojunction Y6/CdS as photoactive material. Compared with single organic semiconductor, Y6, Y6/CdS exhibited higher photoelectric conversion efficiency due to the formation of heterojunction. In the presence of HER2, sandwich immune structure was formed between HER2 aptamer and anti-HER2 antibody (Ab) by specific recognition. The polydopamine (PDA) nanoparticles were used for signal amplification to enhance photocurrent intensity as PDA can act as electron donor to eliminate holes and promote electron–hole pairs separation. The developed PEC sensor displayed a wide detection range of 5–1000 pg mL −1 and a low detection limit of 2.2 pg mL −1 for HER2 (S/N = 3). The sensor was successfully used for the detection of HER2 in serum with recoveries between 94.8 and 104% and relative standard deviations (RSDs) in the range of 1.2–4.3%. Furthermore, the designed immunosensor possessed good stability, selectivity, and reproducibility, which can find potential clinical applications for disease diagnosis. Graphical abstract A “signal-on” photoelectrochemical sensor was reported for human epidermal growth factor receptor 2 detection based on Y6/CdS organic–inorganic heterojunction
研究领域:
生物医学工程
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分析化学
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生物传感器
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癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 3
·332
·
IF 4.034
Xiaodi Tang,Zhengzhi Yin,Xiaoling Lei,Yanbo Zeng,Zulei Zhang,Yixia Lu,Guobao Zhou,Lei Li,Xiaohua Wu
A novel, facile sensor was further developed for microcystin-LR (MC-LR) determination by visible spectroscopy. Antibody-functionalized SiO2-coated magnetic nanoparticles (Fe3O4@SiO2) and aptamer-functionalized polydopamine nanospheres decorated with Cu nanoparticles (PDA/CuNPs) recognized specific sites in MC-LR and then the sandwich-type composites were separated magnetically. The Cu in the separated composites was converted to Cu2+ions in solution and turn-on visible absorption was achieved after reaction with bis(cyclohexanone)oxaldihydrazone (BCO) (λmax= 600 nm). There was a quantitative relationship between the spectral intensity and MC-LR concentration. In addition, under the optimum conditions, the sensor turns out to be a linear relationship from 0.05 to 25 nM, with a limit of detection of 0.05 nM (0.05 μg/L) (S/N= 3) for MC-LR. The sensitivity was dependent on the low background absorption from the off-to-on spectrum and label amplification by the polydopamine (PDA) surface. The sensor had high selectivity, which shows the importance of dual-site recognition by the aptamer and antibody and the highly specific color formed by BCO with Cu2+. The bioassay was complete within 150 min, which enabled quick determination. The sensor was successfully used with real spiked samples. These results suggest it has potential applications in visible detection and could be used to detect other microcystin analogs.Keywords:turn-on visible absorption;dual-site recognition;magnetic separation;microcystin-LR;colorimetric sensor
研究领域:
分析化学
·
环境科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Micro & Nano Letters
·
2018
·Vol. 13
·期 6
·804-806
·
IF 0.841
Cheng Ju,Xiang Gong,Wenhao Song,Yu Zhao,Ruijun Li
This work reported the fabrication of bovine serum albumin stabilised gold nanoclusters (BSA-Au NCs)–graphene oxide nanocomplex (BSA-Au NCs-GO) as a ‘turn-on’ fluorescent probe for the detection of Cd 2+ . The prepared BSA-Au NCs showed an intense red fluorescence, and the fluorescence of BSA-Au NCs was reduced significantly by graphene oxide due to its excellent quenching properties. However, in the presence of 65 µmol l −1 Cd 2+ , the fluorescence intensity recovered to 82.5% of the intensity of BSA-Au NCs, and the fluorescence intensity increased 1.3-fold relative to the BSA-Au NCs-GO nanocomplex. This proposed probe expands the application of BSA-Au NCs, which can be used as a reversible fluorescence nanoswitch for inexpensive, label free and simple detection of Cd 2+ in the aqueous samples.
研究领域:
纳米技术
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 29
·3282-3291
·
IF 2.896
Jiaxin Zhang,Tingting Feng,Jiayu Zhang,Ning Liang,Longshan Zhao
A simple, quick, effective turn-on fluorescence assay for the determination of fipronil (FIP) was built based on the yellow fluorescence of FAM-aptamer and excellent quenching capability of the oxidized single-walled carbon nanohorns (The oxidized SWCNHs). Oxidized SWCNHs with the great advantage of good dispersibility in solution were generated by link to carboxyl group and were added to a specific FAM-aptamer at an optimal concentration to form an “on–off” oxidized SWCNH/FAM-aptamer fluorescent sensor. The structures of the oxidized SWCNHs were verified, and the comprehensive properties were evaluated by characterization techniques. This paper has exploited oxidized SWCNHs as a quenching agent to detect fipronil for the first time. Under the optimized conditions, the limit of detection (LOD) for fipronil was 3 nM, and the recovery of fipronil varied from 88.6% to 112.7% in different real samples with relative standard deviations (RSDs) not more than 5%. The developed method could be successfully applied for the determination of fipronil in tap water, honey and corn samples.
研究领域:
分析化学
·
环境科学
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食品安全
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）