SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yaohong Gu,Qianqian Pang,Zhiting Deng,Zhichao Chen,Yaling Yang
Herein, a novel fluorometric approach is developed for the precise quantification of nicotine. This method utilized the derivatizing reaction between o-phthalaldehyde (OPA) and reduced glutathione (GSH), and derivatized product with tricyclic conjugated structure generate fluorescent emission. And the derivative exhibits a quantum yield of 33.46 % and distinct fluorescence “turn-off” quenching behavior in the presence of Cu 2+ ions due to the GSH-Cu 2+ complex formation. More importantly, when nicotine is introduced reaction system, the fluorescence restored (“turn-on”) based on the nicotine-Cu 2+ complex formation. Therefore, a fluorimetric sensor for nicotine detection is developed with lower detection limit (LOD) of 0.21 μg/mL. The developed method was successfully utilized to analyze nicotine content in electronic cigarette and cigarette bag samples, yielding satisfactory recovery rates ranging from 85.2 % to 111.8 %. The obtained results demonstrated comparable accuracy and confirmed the method's reliability and practical applicability.
研究领域:
分析化学
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生物传感
·
药物代谢
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环境毒理学
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分子诊断
·
转化医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Zihan Wang,Li Zhang,Yumin Hao,Wenjuan Dong,Yang Liu,Shengmei Song,Shaomin Shuang,Chuan Dong,Xiaojuan Gong
The fluorescent sensor, especially ratiometric fluorescent sensor, is one of the most important applications for CQDs , which is becoming a research hotspot. Herein, carbon quantum dots co-doped with nitrogen, phosphorus and chlorine (NPCl-CQDs) were synthesized by acid-base neutralization reaction exothermic carbonization method. The as-fabricated NPCl-CQDs could emit blue fluorescence and possess excellent fluorescence properties. Based on the FRET , multifunctional and ratiometric fluorescent sensors for “on-off-on” sequential determination of riboflavin , Ag + , and Cys with good selectivity and high sensitivity were established. The linear range of riboflavin, Ag + , and Cys are 0.50–10.18 μM and 15.89–27.76 μM, 0.66–1.46 mM and 1.50–4.20 mM, and 0.01–0.15 μM and 0.15–0.36 μM with the limit of detection of 3.50 nM, 26.38 μM, and 0.96 nM, respectively. Furthermore, the sensors were successfully used to determine riboflavin, Ag + , and Cys in tablets, river water, and human urine with the recoveries of 95.2–104.0%, 95.6–102.0%, and 94.8–106.4%, respectively. More importantly, the as-constructed “on-off-on” NPCl-CQDs-based ratiometric fluorescent sensors were applied for detecting riboflavin, Ag + , and Cys in HeLa cells with satisfying results. The finding of this study shows the feasibility and effectiveness of the NPCl-CQDs as the available ratiometric fluorescent sensors for the determination of riboflavin, Ag + , and Cys in real samples and living cells.
研究领域:
分析化学
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纳米技术
·
生物医学工程
·
荧光光谱
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Qin Yingfeng,Huang Ruiqi,Ye Gao-Jie
The analysis of glyphosate is essential to agricultural production, environment protection and public health. Herein, we proposed a fast and convenient “on-off-on” fluorescence platform for sensitive detection of glyphosate via Cu2+ modulated g-C3N4 nanosheets. The fluorescence of the system was quenched by Cu2+. With the presence of glyphosate, the fluorescence could be restored due to the formation of Cu2+- glyphosate complex. The proposed method was cost-effective with label-free and enzyme-free. Moreover, it exhibits high sensitivity with a low detection limit of 0.01 μg/ml. Furthermore, the proposed method has been successfully monitored glyphosate in real samples.
研究领域:
农业科学
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环境科学
·
分析化学
·
公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Guo Wei,Chuanliang Wang,Rui Wang,Xuhui Geng,Jinhua Li,Lingxin Chen,Zhihua Song
In this study, a novel carbon dots-based system was developed for the sequential quantification of Au 3+ and L-cysteine (L-Cys). The system comprises N,F-doped carbon dots (N,F-CDs), a custom-designed miniaturized fluorimeter, and test strips. The N,F-CDs exhibit outstanding optical properties, including a large Stokes shift (127 nm) and high fluorescence intensity. The test strips offer low-cost, low-volume sample consumption, while the miniaturized fluorimeter provides high sensitivity in a compact format. The system demonstrated excellent analytical performance, including high sensitivity (limits of detection: 0.048–0.488 μmol/L for Au 3+ and 8.7–40.9 nmol/L for L-Cys), minimal matrix interference (within ±20 %), high accuracy (97.5 % to 104.5 % for Au 3+ , 97.6 % to 107.5 % for L-Cys), good precision (2.9 % to 4.5 % for Au 3+ , 1.2 % to 7.0 % for L-Cys), robust stability, and strong inter/intra-day reproducibility (relative standard deviations ≤2.1 %). Furthermore, it showed high selectivity for Au 3+ and L-Cys in complex matrices such as tap water, milk, and lemon-flavored beverages.
研究领域:
分析化学
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纳米技术
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环境监测
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生物分析
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2025
·
IF 5.1
Tingjie Jiang,Na Yang,Ziyi Wang,Haoran Zhang,Na Li,Yongying Chen,Mingkai Wei,Xuejie Zhang,Bingfu Lei
In this study, a carbon dot-based ratiometric fluorescence (RF) probe was used as a fluorescent "on-off-on" sensor to detect Fe3+ and the pesticide amitrole (AMT). The RF probe consists of orange carbon dots (O-CDs) and blue carbon dots (B-CDs). B-CDs forms a ground-state complex with Fe3+ which leads to fluorescence quenching, while the addition of AMT restores fluorescence. Based on this "on-off-on" process, a quantitative detection of Fe3+ and AMT is achieved, showing good linearity in the range 0-50 μM and 0-30 μM. The low detection limit of Fe3+ and AMT are as low as 24 nM and 0.18 μM, respectively. In addition, fluorescent color changes are converted into RGB values by a smartphone sensing device., and the concentration of Fe3+ and AMT is determined by colorimetric analysis. The method was further applied to real samples such as lake water, tap water and urine with satisfactory results. Additionally, a portable sensor with a semi-hydrophobic film was fabricated for on-site AMT detection, showcasing practical applicability. This work provides a cost-effective, rapid, and dual-mode strategy for environmental and biological monitoring of Fe³⁺ and AMT.
研究领域:
环境监测
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分析化学
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纳米技术
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生物传感
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荧光光谱学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ouwen Xu,Shuyu Wan,Yuheng Zhang,Jiawei Li,Xiashi Zhu
A dual-"on-off-on" fluorescent probe based on nitrogen-doped carbon quantum dots (BG-NCDs) with the dual-excitation property was developed, which had a blue emission peak at 398 nm and a green emission peak at 518nm when excited at 298nm and 404nm, respectively. BG-NCDs were characterized by TEM, XPS, and FT-IR. The results showed that (1) the Fe (III) and ascorbic acid (AA) could “turn off/turn on” the blue fluorescence of BG-NCDs, the blue fluorescence of BG-NCDs was used as an “on-off-on” fluorescent probe to detecting the Fe (III) and AA in the linear ranges of 0.04–20.00 mg/L and 0.57–10.00 mg/L, the LODs were as low as 0.012 mg/L and 0.19 mg/L; (2) the presence of Cu (II) and glutathione (GSH) could “turn off/turn on” the green fluorescence of BG-NCDs, the “on-off-on” process of green fluorescence of BG-NCDs could be established as a fluorescence probe to detecting Cu (II) and GSH in the linear ranges of 1.08–16.00 mg/L and 2.46–32.00 mg/L, with LODs of 0.36 mg/L and 0.86 mg/L. The method was applied to determination analytes in real samples with satisfactory results. BG-NCDs displayed low cytotoxicity and was multicolored imaging DU145 cells successfully.
研究领域:
材料科学
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分析化学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 42
·5110-5119
·
IF 2.596
Shanshan Wei,Tinghua Li,Xinyu Zhang,Hongyuan Zhang,Chunzhu Jiang,Guoying Sun
The abnormal level of Cu2+ or GSH can cause variety of neurodegenerative diseases in humans. Thus, the selective and sensitive detection of Cu2+ and GSH has inspired intensive research efforts in biological sample analysis fields. Herein, an “on–off–on” fluorescent probe based on nitrogen and sulfur co-doped carbon dots (N,S-CDs) has been successfully prepared for the detection of Cu2+ and GSH. The “turn-off” process of fluorescence in the presence of Cu2+ ions was induced by forming a non-luminescent ground state complex due to the interaction between surface groups of the probe and Cu2+ ions. Moreover, the strong coordination between GSH and Cu2+ could destroy the structure of the complex and restore the fluorescence to “turn-on”. This fluorescent probe had excellent selectivity and high sensitivity toward Cu2+ and GSH with the limits of detection (LODs) of 38 nM and 41 nM. More importantly, the as-prepared N,S-CDs served as an efficient fluorescent probe for not only detecting Cu2+ ions in lake water and tap water, and GSH in BSA solution, but also sensing Cu2+ and GSH in living cells. Therefore, these N,S-CDs could be considered as a promising fluorescence probe candidate for environmental monitoring and biological imaging.
研究领域:
分析化学
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生物化学
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材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-8
·
IF 5.705
Pang Jiawei,Lu Yuexiang,Gao Xinyu,Song Panshu,Yang Fengyi,Liu Yueying
A selective phosphorescent on-off-on probe with long decay lifetime has been designed for the detection of pyrophosphate ions (PPi). The detection scheme is based on the use of europium(III)-modulated Mn(II)-doped ZnS quantum dots capped with N-acetyl-L-cysteine. Both the aggregation of quantum dots and electron transfer induced by Eu(III) ions cause phosphorescence to be quenched (“off” state). Phosphorescence is, however, restored on addition of PPi to the system (“on” state). The effect is attributed to the removal of Eu(III) from the carboxy groups on the surface of the quantum dots owing to the stronger interaction between PPi and Eu(III). A linear relationship exists between phosphorescence intensity (best measured at excitation/emission wavelengths of 316/594 nm) and PPi concentration in the 400 nM to 6000 nM with a detection limit of 145 nM. An additional attractive feature is provided by the long-lived phosphorescence (1920 μs) of the quantum dots. It can be used to eliminate interference by short-lived fluorescence in biological samples by performing time resolved measurements. The probe was applied to the determination of PPi in spiked in urine samples and gave recoveries in the range from 98 to 105% with RSDs of <2.0%.
Schematic of a long-lived phosphorescent on-off-on probe for the sensitive and selective detection of pyrophosphate ions (PPi). It is based on the use of Eu(III)-modulated Mn(II)-doped ZnS quantum dots (QDs). Phosphorescence is quenched of QDs after the addition of Eu 3+ but restored after the addition of PPi.
研究领域:
分析化学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Bo Liu,Yonghao Ge,Yahui Lu,Yibo Huang,Xiaoru Zhang,Xunyi Yuan
This work develops a novel photoelectrochemical sensor for the detection of carcinoembryonic antigen (CEA) based on the composite of UCNPs with semiconductors and conformational changes in the DNA structure . Firstly, SnS 2 , ZnIn 2 S 4 and UCNPs were assembled on the surface of the ITO electrode. Then Au NPs were dropped, which could facilitate the coupling of CdSe NPs modified DNA1 via Au–S bond, giving an ITO/SnS 2 /ZnIn 2 S 4 /UCNPs/CdSe heterojunction structure. When irradiated with 980 nm near-infrared (NIR) light, the UV–visible light emitted by the UCNPs could excite the nanocomposite , producing an enhanced photoelectric reaction. Subsequently, CEA aptamer and DNA2-modified SiO 2 were added to form a Y-shaped DNA structure. At this time, the photocurrent was significantly reduced by the combination of the light-blocking effect of SiO 2 and the departure of CdSe NPs from the electrode surface . When the target CEA was added, the recognition between CEA and the aptamer led to the collapse of the Y-shaped DNA structure, the restoration of hairpin DNA and the proximity of CdSe to the electrode. Accordingly, the photocurrent signals enhanced again. Under optimal experimental conditions, the detection limit as low as 0.3 pg mL −1 was obtained with good selectivity , achieving a sensitive “on-off-on” photoelectrochemical sensor for CEA detection.
研究领域:
分析化学
·
生物传感
·
分子诊断
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Lizhu Fu,Yujiao Hou,Haipeng Guo,Chang Liu,Ying Li,Boyu Jia,Lidong Zhou,Weijun Kong
Development of highly efficient and sensitive methods for trace detection of pesticides in foods is in great need. In this study, a signal “on–off-on” fluorescent nanosensor was proposed based on gold nanoparticles (AuNPs) and biomass derived nitrogen-doped carbon dots (N-CDs) for accurate qualitation and quantitation of thiram in hawthorn. The water-soluble orange peel-derived N-CDs were obtained through a facile one-step microwave-assisted synthesis strategy. The fluorescence of biomass N-CDs could be effectively quenched by AuNPs through inner filter effect (IFE), and recovered when AuNPs were aggregated. In the presence of thiram, N-CDs specially bound with AuNPs through Au-S bonds, causing the aggregation of AuNPs with a visible color change from red to blue, accompanying with the release of N-CDs and a recovery of fluorescence. The restored fluorescence intensity was relevant with the added amount of thiram in a concentration-dependent manner for reliable quantitation. Under the optimized conditions, the newly-developed fluorescent nanosensor exhibited high selectivity for thiram out of other six interfering pesticides, as well as excellent sensitivity with an ultralow detection limit of 4.7 ng/mL and a wide detection range of 10–200 ng/mL. The practical application of the established nanosensor in the spiked hawthorn samples confirmed satisfactory recoveries of 102.22–107.57% with relative standard deviations (RSDs) lower than 5%. The results indicated the feasibility, practicality and wide application prospect of the proposed fluorescent nanosensor for thiram in foods and other commodities.
研究领域:
分析化学
·
纳米技术
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Zhichang Ma,Jing Sun,Hemeiling Cai,Junwei Di
A photoelectrochemical (PEC) sensor, utilizing the semiconductor BiVO 4 and gold nanoparticles (AuNPs) modified with glutathione (GSH), was successfully fabricated for the sensitive detection of Pb 2+ ions. The calcinated BiVO 4 -modified indium tin oxide (ITO) electrode (ITO/BiVO 4 ) generated a low cathodic photocurrent response under light irradiation . The photocurrent at ITO/BiVO 4 /AuNPs photoelectrode increased significantly due to the plasmon enhancement of AuNPs and the electron transfer. This enhancement photocurrent intensity was largely suppressed by introducing GSH to form ITO/BiVO 4 /AuNPs/GSH. It minimized the base photocurrent of the photoelectrode. Upon the addition of Pb 2+ ions in the cell, they were adsorbed on the electrode surface through the interaction with the two carboxyl groups in GSH. Subsequently, Pb 2+ ions, serving as electron acceptors , underwent reduction under light irradiation, leading to increase in photocurrent intensity again. Therefore, a signal "on-off-on" PEC sensor was developed for the detection of Pb 2+ ions. The concentration of Pb 2+ ions show a good linearity between 0.1 pM and 10 μM with a detection limit of 0.08 pM (S/N = 3). The prepared PEC sensor shows high sensitivity, a wide linear range, and good selectivity. This work provides a promising platform for the analytical detection of heavy metal ions .
研究领域:
分析化学
·
环境科学
·
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Wenjing Wang,Jing Wu,Yuxin Xing,Zonghua Wang
Herein, a kind of red emissive carbon dots (BNCDs) based on 1,1′-binaphthyl-2,2′-diamine (BNDM) and citric acid as carbon source were prepared through one-pot solvothermal method . The synthesized BNCDs have uniform size distribution, photobleaching resistance, and excitation wavelength-independent emission performance with the optimal fluorescence at λ ex /λ em = 540/603 nm. The composite films of BNCDs in polyvinyl alcohol (PVA) matrix exhibited bright red emission under ultraviolet irradiation , indicating their potential in solid-state luminescence. Also, BNCDs shows unique solvent-dependent phenomenon, the optical behavior can be tuned by the change of solvent polarity. Another striking observation was that BNCDs can be applied as a fluorescence sensor for highly sensitive quantitative detection of trace water in organic solvents because it’s polarity-sensitive property. Through fluorescence “on–off–on” mechanism, BNCDs could be used as a fluorescence sensor for detection of Fe 3+ and F - with LOD of 0.219 and 0.0104 μmol L −1 , respectively. This strategy offers an efficient approach for preparing red emissive carbon dots and lays a foundation for its multi-functional application.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
光学传感
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）