JOURNAL OF SEPARATION SCIENCE
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2013
·Vol. 37
·期 5
·587-594
·
IF 2.591
Ting Yang,Chao Ma,Huaixia Chen,Yajie Zhang,Xueping Dang,Jianlin Huang
An IPC-imprinted (IPC is isoprocarb) poly(methacrylic acid)/SiO 2 hybrid monolithic column was prepared and applied for the recognition of the template. The hybrid monolithic column was synthesized in a micropipette tip using methyltrimethoxysilane as the inorganic precursor, 3-(methacryloxy)propyltrimethoxysilane as the coupling agent, and ethylene glycol dimethacrylate as the cross-linker. The synthesis conditions, including the porogenic solvent, coupling agent, volume ratio of the inorganic alcoholysate and organic part, were optimized. The prepared monolithic column was characterized by SEM and FTIR spectroscopy. A simple, rapid, and sensitive method for the determination of IPC in rice using the imprinted monolithic column microextraction combined with HPLC was developed. Several parameters affecting the sample pretreatment were investigated, including the eluent, washing solution, and loading sample volume. The linearity of the calibration curve was observed in the range of 9.0–1000 μg/kg for IPC in rice with the correlation coefficient ( r 2 ) of 0.9983. The LOD was 3.0 μg/kg (S/N = 3). The assay gave recovery values ranging from 91 to 107%. The proposed method has been successfully applied for the selective extraction and sensitive determination of IPC in rice and a satisfactory result was obtained.
研究领域:
分析化学
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食品科学
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PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
·
IF 7.129
Shanghong Wang,Xue Han,Tingting Yu,Yulong Liu,Hongying Zhang,Huiling Mao,Chengyu Hu,Xiaowen Xu
Isoprocarb is a widely used carbamate insecticide in agriculture and aquaculture. Overuse of isoprocarb always leaves toxic residues in soil and water, however, the potential ecotoxicity of isoprocarb to organisms is still confusing. In this study, zebrafish embryo was used as a model to evaluate the toxicity of isoprocarb. Zebrafish embryos (96 hpf) were separately exposed at different concentrations of isoprocarb. The mortality rate, hatchability rate, average heart beat of the zebrafish embryo were separately calculated. Our results suggested that exposure to isoprocarb induced developmental toxicity in zebrafish embryos. HE staining showed that exposure to isoprocarb caused developmental defect in the hindbrain of zebrafish embryos. As expected, the behavioral analysis also showed that the motor ability of zebrafish embryos were significantly inhibited following exposure to isoprocarb. In terms of mechanism, The expressions of genes involved in neurodevelopment signaling pathways, such as foxo3a , gfap , syn2a , elavl3 and sox19b , were inhibited in zebrafish embryos after exposure to isoprocarb. The acetylcholinesterase (AChE) activity was also reduced in isoprocarb-treated zebrafish embryos. Moreover, oxidative stress was induced by increasing the reactive oxygen species (ROS) level and decreasing the activity of antioxidant enzyme (SOD) after exposure to isoprocarb. Expectedly, acridine orange (AO) staining and the detection of some apoptosis-related genes revealed that oxidative stress resulted in apoptosis. In short, the expressions of genes associated with the neurodevelopmental signaling pathway are inhibited, and oxidative stress is also induced in zebrafish embryos after exposure to isoprocarb, which may be the molecular basics of isoprocarb-induced neurotoxicity in zebrafish embryos.
研究领域:
生态毒理学
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发育生物学
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分子毒理学
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高影响因子
ANALYTICA CHIMICA ACTA
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2025
·
IF 6
Menghan Liu,Xiuqi Fan,Zhe Li,Guangyu Li,Tiangang Zhu,Ruyu Zhang,Lihua Lu
To develop long-lifetime luminescent Iridium(III) [Ir(III)] complexes, two novel series ( a1 – a4 and b1 – b4) were designed and synthesized, and their structure-photophysical property relationships were systematically investigated. Notably, introducing a sulfur (S) atom simultaneously extends the luminescence lifetime and enhances the quantum yield of these complexes. Among them, b3 stands out as an exceptional time-resolved emission spectroscopy (TRES) probe due to its prolonged lifetime and high quenching efficiency by polydopamine (PDA). By leveraging dual mechanisms, PDA-mediated quenching of b3 and thiocholine (TCh)-inhibited dopamine (DA) polymerization, a sensitive luminescent platform for malathion (MAL) detection was developed. The resulting detection platform achieves an ultra-low limit of detection (LOD) 0.32 nM within a linear range of 0.001–15.0 μM. Notably, the long-lived b3 (τ = 1.665 μs) permits effective MAL detection even in a highly fluorescent RhB matrix using TRES (LOD = 13.2 nM), whereas steady-state emission spectra (SSES)-based detections fail under such conditions. The successful application of b3 in real-sample analysis demonstrates the great potential of Ir(III) complexes in TRES-based detection. This work not only expands the repertoire of TRES probes but also presents a highly effective luminescent platform for pesticide detection.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Lin-Lin Zhang,Zeng Chen,Li Li,Wuying Yang,Dan Wang,Yanping Hong,Kaijie Tang,Jiaxin Hong,Lu-Ying Duan,Limin Lu
Developing a multifunctional sensor that can detect food-related multi-analytes is significant for food safety but remains challenging due to the difficulty of integrating multiple functions into one sensor. Herein, a dual-luminescent europium metal-organic framework (EuMOF) was elaborately synthesized through a simple self-assembly process, during which Eu 3+ coordinated with 2-hydroxyterephthalic acid (HTPA) and 1,10-phenanthroline that delivered strong characteristic fluorescence of HTPA and Eu 3+ . The resultant EuMOF exhibited good detection performance toward Sn 4+ and amitraz with different response behaviors. As for target Sn 4+ , the remarkably enhanced fluorescence from HTPA was observed and the fluorescence for Eu 3+ decreased significantly, which was attributed to the structural disintegration of EuMOF caused by Sn 4+ . Whereas, the presence of amitraz weakened both luminescence of HTPA and Eu 3+ owing to the synergistic function of inner filter effect and photoinduced electron transfer. Furthermore, an intelligent and miniature sensor that incorporated EuMOF-based test paper with color recognition application was exploited for portable visual analyzing Sn 4+ and amitraz in real samples, affording great possibilities for on-site monitoring of food hazards.
研究领域:
材料科学
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分析化学
·
食品安全
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传感器与生物传感器
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环境监测
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 10.6
Yuqing Cheng,Tian Tian,Donghui Song,Yu Ma,Zhichun Bi,Xinjian Zhang,Ling Zhang,Yongxin Li,Hui Huang
Sulfonylurea herbicides (SUs) are widely used in agriculture owing to their high herbicidal efficacy and low mammalian toxicity. However, their persistence results in residues that pose potential risks to the environment and human health, making it essential to develop rapid and accurate detection techniques for these herbicides. Herein, we designed bionic nanozymes inspired by the structure of acetolactate synthase (ALS), the primary target enzyme of SUs in plants. Using the ALS cofactor thiamine diphosphate and key binding amino acids (arginine, proline, and tryptophan) as ligands, we synthesized bionic nanozymes that exhibited excellent peroxidase-like activity. SUs significantly enhanced the activity of the bionic nanozymes, and the degree of enhancement varied among different SUs. In contrast, organophosphate pesticides (OPs), carbamate pesticides (CPs), and other tested pesticides did not induce such enhancement. Therefore, we constructed a six-channel colorimetric sensor array and achieved 100% accurate discrimination of five SUs across a concentration range of 1–100 µg/mL. Furthermore, the SUs in the four real grain samples and the blind sample were also accurately distinguished by the array. This study not only provides an efficient method for rapid detection of SUs, but also opens new avenues for the bionic design of nanozymes aimed at the precise identification of specific pesticide molecules in complex matrices.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2025
·
IF 11.3
Zhenhua Dai,Jihong Xu,Jian Guan,Mingyang Feng,Yang Liu,Cuili Xing,Xuanying Cai,Shuchen Wang,Lushi Lian,Hongyu Dong,Zhiyong Jason Ren,Wei Shi,Alicia Kyoungjin An,Shifa Zhong,Xiaohong Guan
Chemical oxidation is pivotal in remediating organic pollutants in aquatic systems; however, it frequently yields transformation products (TPs) with potential toxicological profiles surpassing those of the parent pollutants. Comprehensive identification of these TPs is imperative for environmental risk assessment and optimization of oxidation methodologies. Traditional experimental approaches for TP elucidation are often hindered by substantial financial and technical constraints, limiting their applicability in high-throughput scenarios. Here, we introduce TP-Transformer, an advanced deep learning framework designed to predict both the structures of TPs and their corresponding formation pathways. Trained on Chem_Oxi_2K, a meticulously curated data set comprising 2780 pollutant degradation reactions, TP-Transformer achieved a notable accuracy of 86.28% in TP prediction. The model adeptly reconstructs complete degradation pathways, addressing the intricate challenge of pathway elucidation. Attention analyses indicate that the TP-Transformer discerns reactive moieties within substrates and correlates them with specific reaction conditions, emulating expert-level chemical reasoning. Experimental validations corroborate the model’s robustness, with accurate TP predictions ranging from 80.20 to 92.86% for five pollutants absent from the training data set. These findings underscore TP-Transformer’s potential to transform environmental chemistry by offering a scalable, precise, and efficient alternative to traditional experimental methodologies, thereby enhancing water treatment strategies and safeguarding ecological and human health.
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yufan Yuan,Danping Li,Huajun Huang,Jinbao He,Chenglong Yu,Yanpeng Gao,Davide Vione,Hansun Fang
The direct photodegradation quantum yields ( Φ ) of five representative aromatic carbamate pesticides - carbaryl, carbofuran, propoxur, isoprocarb, and metolcarb - were examined in both aqueous and non-aqueous solutions, the latter mimicking hydrophobic environments such as leaf surfaces. For carbaryl, carbofuran, isoprocarb, and metolcarb, the Φ values generally followed the order Φ water < Φ MeOH < Φ n - hexane , while propoxur showed a different trend, Φ MeOH < Φ n-hexane < Φ water . Scavenging and laser flash photolysis experiments, combined with quantum chemical calculations, were used to clarify the photodegradation mechanisms. Photodegradation is primarily initiated by the singlet excited state (S*), with the triplet state (T*) also contributing in compounds with conjugated structures, such as carbaryl. Upon excitation, MCAEs generated both radical cations (S •+ ) and phenoxyl radicals (S-O • ), and S •+ would convert to S-O • subsequently. S-O • is predominantly generated through the cleavage of C-O bonds in ester groups, subsequently abstracting hydrogen from solvent molecules. The reactivity of hydrogen donors in these solvents follows the order: -CH 2 - > -CH 3 > -OH. For propoxur, the ether group also contributes to the formation of S-O • , which further reacts with H 2 O and enhances degradation in aqueous environments. Solvent polarity had a minimal effect on photodegradation. This comparative study of degradation in aqueous and nonaqueous phases provides insights for designing and selecting pesticides that are effective during use in nonaqueous environments, such as on leaf surfaces, yet degrade rapidly in aqueous environments in the post-application phase.
研究领域:
环境科学
·
化学
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高影响因子
Toxics
·
2024
·Vol. 12
·期 9
·654
·
IF 3.9
Zihan Niu,Chao Chen,Qijun Ruan,Yingming Duan,Shuqin Liu,Da Chen
Studying the interaction between pesticide contamination in the plant system and the dissolved organic matter (DOM) composition is important to understand the impact of pesticides and plants on the ecological function of DOM. The present study investigated the effects of DOM on the bioaccumulation and biotransformation of carbamates in plants, carbamate exposure on DOM composition, and plant root secretion on the interaction between DOM and carbamates. The concentrations of carbamates and their metabolites in living cabbage plants were continuously tracked through an in vivo analytical method. The presence of DOM was found to reduce the highest bioconcentrations and shorten the time it took to reach the highest bioaccumulated amounts of isoprocarb and carbofuran in plants, while it showed no significant effect on the uptake behavior of carbaryl. DOM profiling results indicated that carbamate exposure substantially decreased the number and molecular diversity of DOM. Notably, plant root secretion alleviated carbamate-induced DOM molecular alterations by inducing a higher turnover rate of DOM compared to that in the uncontaminated group, highlighting the role of plants in mitigating the effects of exogenous pesticide exposure on DOM composition and maintaining DOM molecular homeostasis.
研究领域:
环境科学
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植物学
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高影响因子
Heliyon
·
2025
·
IF 3.6
Xinyue Li,Yue Wang,Cheng Chen,Chao Tian,Xiaoli Yu,Jinglei Liu,Qin Li,Shuling Wang
The pollution of carbamate pesticides (CMs) has seriously threatened human health. Here, a sustainable biochar derived from lotus seedpod (LSP) was prepared to remove residual CMs. Among various raw materials, lotus seedpod-derived biochar (LSPB) showed better surface morphology, high porosity and more surface functional groups to mediate the best adsorption performance of CMs. When the LSP/H 3 PO 4 ratio, carbonization temperature and time was defined as 1:5, 700°C and 60 min, respectively, LSPB obtained the highest adsorption capacity and good yield. The SEM micrographs, XRD and FTIR showed that LSPB was of porous and amorphous carbon structure, and increased functional groups for more surface adsorption sites. The adsorption efficiency of LSPB was considerably higher for adsorbent dosage of 0.5 g/L, solution temperature of 20°C, initial CMs concentration of 10 μg/mL, and adsorption time of 30 minutes. The Elovich adsorption kinetic model indicated the potential heterogeneous surface adsorption mechanism of LSPB. Adsorption thermodynamic showed that the CMs adsorption on LSPB was spontaneous and endothermic. LSPB displayed favorable recovery performance, and can be effectively used for five cycles. LSPB showed potential adsorption performance in different CMs-residual waters, and is even superior to that of some commercial adsorption products. Based on its favorable adsorption performance and low-cost, this sustainable LSPB may be a promising CMs residues adsorbent for removal of it from water.
研究领域:
环境科学
·
农业科学
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 9
·1-10
·
IF 6.232
Guo Liying,Wu Jingyu,Xing Fei,Liu Weihua,Hao Lin,Wang Chun,Wu Qiuhua,Wang Zhi
Graphene-carbon nanosphere composite (G@CNS) was prepared via a simple hydrothermal method. The G@CNS nanocomposite was characterized by X-ray diffraction, scanning electron microscope, surface area, and porosity analysis. The G@CNS was applied as a new sorbent for solid-phase extraction of five carbamate pesticides (tsumacide, carbaryl, isoprocarb, bassa, diethofencarb) prior to quantitative determination by high-performance liquid chromatography with ultraviolet detection at 208 nm. Some experimental parameters including desorption conditions, sample pH, sample volume, and loading rate were studied carefully. Under the optimized condition, the method provided good linearity ranging from 0.3 to 100.0 ng mL −1 with low limits of detection of 0.10–0.20 ng mL −1 for grape juice, 0.10–0.30 ng mL −1 for blend fruit juice, and 0.10–0.20 ng mL −1 for water sample. Good method recoveries (80.2–110%) with relative standard deviations less than 7.2% and high enrichment factors (167–293) were achieved. Results demonstrated that this novel G@CNS can serve as a promising alternative sorbent for more applications.
In this work, a graphene-carbon nanosphere (G@CNS) composite was synthesized via a simple hydrothermal method. Then, the G@CNS was served as a novel sorbent for solid-phase extraction of five carbamate pesticides (tsumacide, carbaryl, isoprocarb, bassa, diethofencarb) in juice and environmental water samples, followed by their quantitative analysis with high-performance liquid chromatography-ultraviolet detection.
研究领域:
材料科学
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分析化学
·
环境科学
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高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Xu Jing,Huihui Wang,Xin Huang,Zhenjia Chen,Junling Zhu,Xiaowen Wang
This research used a digital image colorimetry (DIC) method to detect carbaryl in food samples using effervescence-assisted liquid phase microextraction based on solidification of switchable hydrophilicity solvent combined with a microfluidic thread-based analytical device (EA-LPME-SSHS-μTAD). 1-naphthol, the hydrolysate of carbaryl, was extracted into octanoic acid by the adjustment of pH values of the sample solution and separated through solidification in an ice bath. Then 1-naphthol contained in the extracted solution was coupled with 4-methoxybenzenediazonlum tetrafluoroborate (MBDF) fixed on the μTAD to produce tangerine compounds. The inherent colour variation was captured by a smartphone and processed to calculate the intensity ( I ). Under the optimal conditions, the limit of quantification was within 0.020–0.027 mg kg −1 . The recovery was varied in the range from 92.3% to 105.9% with a relative standard deviation (RSD) below 5%. The developed method provides an alternative strategy to extract and detect pesticides for food samples.
研究领域:
分析化学
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食品安全检测
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Linfeng Chen,Xike Tian,Yong Li,Liqiang Lu,Yulun Nie,Yanxin Wang
Accurate screening of organophosphorus and carbamates pesticides from the complex real sample is crucial for water quality analysis and food safety control. Herein, a simple, low-cost and accurate pesticides screening method based on a high-throughput optical array system assembled by multiple cholinesterases (ChE) and thiocholine (TCh) sensors is described. The detection mechanism is that the inhibition of ChE activity by pesticides reduces the TCh produced by the hydrolysis of butyryl/acetylthiocholine iodide, thus changing the fluorescence intensity of TCh sensor. The diverse response of ChEs to pesticides and different affinity of sensors to TCh ensure the high-throughput and distinguishable signal output, which allow the establishment of high discrete pesticide database with intra-cluster agglomeration and inter-cluster dispersion. By using the database, the screening of unknown real contaminated samples were successfully operated, and the screened pesticide species and concentrations were consistent with high-performance liquid chromatography. This screening strategy demonstrates the feasibility of replacing existing complex mass spectrometry-based screening strategy with simple optical analysis, providing a new idea for the development of simple accurate screening technologies for widespread organic pollutants including pesticides.
研究领域:
分析化学
·
环境科学
·
食品安全
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高影响因子
危险品化学品经营许可证（带存储）