LANGMUIR
·
2025
·
IF 3.9
Yu Yan,Xianchi Zhou,Zuolong Liu,Zihao Zhu,Wenzhong Cao,Kexin Chen,Jian Ji,Peng Zhang
The foreign body response (FBR) presents a significant challenge to biomedical implants, leading to fibrotic capsule formation that compromises implant functionality. In this study, we report a straightforward method for fabricating stable anti-fibrotic polyelectrolyte coatings on implant surfaces using industrialized ultrasonic spraying technology. The coating thickness and surface charge can be adjusted through variations in spraying time and polyelectrolyte ratio, respectively. We investigate the fibrotic response of polyelectrolyte-coated implants with varying surface charges and thicknesses. Our findings reveal that surface charge significantly influences the fibrotic response, while electronegative polyelectrolyte coatings most effectively inhibit FBR compared to electrically neutral, positively charged, and uncoated surfaces. Meanwhile, coating thickness beyond 10 μm resulted in thinner capsules than coatings at a 1 μm or nanometer scale. The simple and versatile polyelectrolyte coating method reported here holds great potential to enhance and extend the functionality of implants in a mass-produced manner by mitigating host responses to implantable biomaterials.
研究领域:
生物医学工程
·
材料科学
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Mengxue Zhou,Zihao Wang,Mengyu Li,Qi Chen,Shengmin Zhang,Jianglin Wang
The development of small-diameter vascular grafts (SDVGs) still faces significant challenges, particularly in overcoming blockages within vessels. A key issue is the foreign-body response (FBR) triggered by the implants, which impairs the integration between grafts and native vessels. In this study, we applied an interfacial infiltration strategy to create a stable, hydrophilic, and passivated hydrogel coating on SDVGs. This coating effectively resisted FBR and improved integration between the grafts and host tissue. We also incorporated anthocyanins, an antioxidant, into the hydrogel network to mitigate oxidative stress and promote endothelialization. The hydrogel coating exhibited excellent stability, retaining its integrity during continuous flushing over 15 days. Anthocyanins were released in response to reactive oxygen species (ROS), reducing inflammation and enhancing vascularization in a mouse subcutaneous implantation model. In a rabbit carotid artery replacement model, the SDVGs exhibited rapid endothelialization, guided vascular remodeling, and inhibited calcification, showing strong potential for clinical application. This study presents a straightforward and effective approach to improve the patency rate, endothelialization, and anti-calcification properties of SDVGs by equipping them with a protective anti-FBR and anti-inflammation hydrogel layer.
研究领域:
生物医学工程
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组织工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2025
·
IF 4.7
Yujie Gao,Xiangyu Dang,Ahai Zhu,Zhize Chen
Hydrogels exhibiting excellent biocompatibility and resistance to foreign body response (FBR) hold significant promise in the biomedical field. In this study, we developed a photopolymerized hydrogel named poly(3,3′-((2-(2-((3-methacrylamidopropyl)amino)-2-oxoethyl)-4-((3-methacrylamidopropyl)carbamoyl)-2,4-dimethylhexanedioyl)bis(azanediyl))bis(N,N-dimethylpropan-1-amine oxide))(PIAPMANOX), whose prepolymer is based on poly(itaconic acid)(PIA) and is grafted with varying ratios of zwitterionic trimethylamine N-oxide (TMAO) derivatives and N-(3-aminopropyl)methacrylamide hydrochloride (APMA). The TMAO derivatives possess the ability to form a compact hydration layer due to their distinctive properties─namely, the minimal spacing between zwitterionic groups, the robust hydrogen bonding interactions, the smallest net charge, and the lowest dipole moment. These characteristics collectively promote the establishment of a tightly packed hydration shell that effectively inhibits nonspecific adsorption of substances such as proteins and cells. Meanwhile, APMA contributes a carbon–carbon double bond that promotes photoinitiated radical polymerization for constructing a three-dimensional hydrogel network. Among the various materials studied, the hydrogel PIAPMANOX, which incorporates the zwitterionic TMAO, demonstrates exceptional performance in terms of protein and cell rejection. The hydrogel was implanted into the peritoneal cavity of mice. Experimental results demonstrated that this hydrogel significantly reduced FBR, with no notable inflammatory reaction observed and a decrease in fibrosis. Therefore, the PIAPMANOX series introduces innovative nontoxic zwitterionic materials to the field of biomaterials.
研究领域:
生物材料学
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组织工程学
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医学材料科学
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产品关联
DOI
高影响因子
Applied Materials Today
·
2023
·
IF 8.3
Chuang Liu,Ziyang Liu,Kexin Wang,Yuting Sun,Qianying Liu,Xun Sun,Tingting Yan,Qiang Yang,Xinlong Ma,Huan Zhou,Lei Yang
Though acknowledged as the essential material in bone bleeding control with advantages of low cost, excellent malleability and smooth consistency for operation, traditional bone wax is associated with adverse effects such as foreign body reaction, granuloma growth, and bone healing failure. Herein, we presented a novel ready-to-use bone wax (NBW) based on DCPA (CaHPO 4 ) granules (DCPA-G) and modified waxy starch (MWS), proposed to overcome problems of bone wax but preserving its impressive workability in hemorrhage control. Thanks to rheological and adhesive properties of MWS after exposure to water, NBW was found to be cohesive, malleable, water content dependent tissue adhesive and liquid sealing abilities. Moreover, after hardening under humid environment due to the interaction between MWS and DCPA-G, NBW exhibited compressive strength that is comparable to that of cancellous bone. After smeared to defect model, water leakage was avoided by NBW. Besides, this material showed superior hemostasis and osteogenesis ability over traditional bone wax in vitro . Simultaneously, in vivo tests confirmed the efficiency of NBW in bleeding control and bone regeneration, indicating it a promising candidate in bleeding bone treatment.
研究领域:
生物材料
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组织工程
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骨科
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止血
·
骨再生
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DOI
高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Xianchi Zhou,Wenzhong Cao,Yongcheng Chen,Zihao Zhu,Yuxian Lai,Zuolong Liu,Fan Jia,Zhouyu Lu,Haijie Han,Ke Yao,Youxiang Wang,Jian Ji,Peng Zhang
Polymeric elastomers are widely utilized in implantable biomedical devices. Nevertheless, the implantation of these elastomers can provoke a robust foreign body response (FBR), leading to the rejection of foreign implants and consequently reducing their effectiveness in vivo. Building effective anti-FBR coatings on those implants remains challenging. Herein, we introduce a coating-free elastomer with superior immunocompatibility. A super-hydrophilic anti-fouling zwitterionic layer can be generated in situ on the surface of the elastomer through a simple chemical trigger. This elastomer can repel the adsorption of proteins, as well as the adhesion of cells, platelets, and diverse microbes. The elastomer elicited negligible inflammatory responses after subcutaneous implantation in rodents for 2 weeks. No apparent fibrotic capsule formation was observed surrounding the elastomer after 6 months in rodents. Continuous subcutaneous insulin infusion (CSII) catheters constructed from the elastomer demonstrated prolonged longevity and performance compared to commercial catheters, indicating its great potential for enhancing and extending the performance of various implantable biomedical devices by effectively attenuating local immune responses. Statement of significance The foreign body response remains a significant challenge for implants. Complicated coating procedures are usually needed to construct anti-fibrotic coatings on implantable elastomers. Herein, a coating-free elastomer with superior immunocompatibility was achieved using a zwitterionic monomer derivative. A pure zwitterionic layer can be generated on the elastomer surface through a simple chemical trigger. This elastomer significantly reduces protein adsorption, cell and bacterial adhesion, and platelet activation, leading to minimal fibrotic capsule formation even after six months of subcutaneous implantation in rodents. CSII catheters constructed from the PQCBE-H elastomer demonstrated prolonged longevity and performance compared to commercial catheters, highlighting the significant potential of PQCBE-H elastomers for enhancing and extending the performance of various implantable biomedical devices.
研究领域:
生物医学工程
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材料科学
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免疫学
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组织工程
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产品关联
DOI
PubMed
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2023
·Vol. 120
·期 3
·e2213837120
·
IF 11.1
Yueqi Ni,Haoning Qi,Fanyu Zhang,Shuting Jiang,Qinchao Tang,Wenjin Cai,Wenting Mo,Richard J. Miron,Yufeng Zhang
Implants are widely used in medical applications and yet macrophage-mediated foreign body reactions caused by implants severely impact their therapeutic effects. Although the extensive use of multiple surface modifications has been introduced to provide some mitigation of fibrosis, little is known about how macrophages recognize the stiffness of the implant and thus influence cell behaviors. Here, we demonstrated that macrophage stiffness sensing leads to differential inflammatory activation, resulting in different degrees of fibrosis. The potential mechanism for macrophage stiffness sensing in the early adhesion stages tends to involve cell membrane deformations on substrates with different stiffnesses. Combining theory and experiments, we show that macrophages exert traction stress on the substrate through adhesion and altered membrane curvature, leading to the uneven distribution of the curvature-sensing protein Baiap2, resulting in cytoskeleton remodeling and inflammation inhibition. This study introduces a physical model feedback mechanism for early cellular stiffness sensing based on cell membrane deformation, offering perspectives for future material design and targeted therapies.
研究领域:
生物医学工程
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免疫学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Xianchi Zhou,Wenzhong Cao,Yongcheng Chen,Zihao Zhu,Yifeng Chen,Yanwen Ni,Zuolong Liu,Fan Jia,Zhouyu Lu,Yang Ye,Haijie Han,Ke Yao,Weifeng Liu,Xinyue Wei,Shengfu Chen,Youxiang Wang,Jian Ji,Peng Zhang
The foreign body response (FBR) to implanted biomaterials and biomedical devices can severely impede their functionality and even lead to failure. The discovery of effective anti-FBR materials remains a formidable challenge. Inspire by the enrichment of glutamic acid (E) and lysine (K) residues on human protein surfaces, a class of zwitterionic polypeptide (ZIP) hydrogels with alternating E and K sequences to mitigate the FBR is prepared. When subcutaneously implanted, the ZIP hydrogels caused minimal inflammation after 2 weeks and no obvious collagen capsulation after 6 months in mice. Importantly, these hydrogels effectively resisted the FBR in non-human primate models for at least 2 months. In addition, the enzymatic degradability of the gel can be controlled by adjusting the crosslinking degree or the optical isomerism of amino acid monomers. The long-term FBR resistance and controlled degradability of ZIP hydrogels open up new possibilities for a broad range of biomedical applications.
研究领域:
生物材料
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生物医学工程
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2022
·Vol. 13
·期 1
·1-13
·
IF 17.694
Yao Mengmeng,Wei Zhijian,Li Junjin,Guo Zhicheng,Yan Zhuojun,Sun Xia,Yu Qingyu,Wu Xiaojun,Yu Chaojie,Yao Fanglian,Feng Shiqing,Zhang Hong,Li Junjie
Zwitterionic hydrogels exhibit eminent nonfouling and hemocompatibility. Several key challenges hinder their application as coating materials for blood-contacting biomedical devices, including weak mechanical strength and low adhesion to the substrate. Here, we report a poly(carboxybetaine) microgel reinforced poly(sulfobetaine) (pCBM/pSB) pure zwitterionic hydrogel with excellent mechanical robustness and anti-swelling properties. The pCBM/pSB hydrogel coating was bonded to the PVC substrate via the entanglement network between the pSB and PVC chain. Moreover, the pCBM/pSB hydrogel coating can maintain favorable stability even after 21 d PBS shearing, 0.5 h strong water flushing, 1000 underwater bends, and 100 sandpaper abrasions. Notably, the pCBM/pSB hydrogel coated PVC tubing can not only mitigate the foreign body response but also prevent thrombus formation ex vivo in rats and rabbits blood circulation without anticoagulants. This work provides new insights to guide the design of pure zwitterionic hydrogel coatings for biomedical devices.
研究领域:
生物材料
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生物医学工程
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
Bioactive Materials
·
2024
·
IF 18
Xianchi Zhou,Hongye Hao,Yifeng Chen,Wenzhong Cao,Zihao Zhu,Yanwen Ni,Zuolong Liu,Fan Jia,Youxiang Wang,Jian Ji,Peng Zhang
Implantable biomaterials and biosensors are integral components of modern medical systems but often encounter hindrances due to the foreign body response (FBR). Herein, we report an albumin coating strategy aimed at addressing this challenge. Using a facile and scalable silane coupling strategy, human serum albumin (HSA) is covalently grafted to the surface of polydimethylsiloxane (PDMS) implants. This covalently grafted albumin coating remains stable and resistant to displacement by other proteins. Notably, the PDMS with covalently grafted HSA strongly resists the fibrotic capsule formation following a 180-day subcutaneous implantation in C57BL/6 mice. Furthermore, the albumin coating led to reduced recruitment of macrophages and triggered a mild immune activation pattern. Exploration of albumin coatings sourced from various mammalian species has shown that only HSA exhibited a promising anti-FBR effect. The albumin coating method reported here holds the potential to improve and extend the function of silicone-based implants by mitigating the host responses to subcutaneously implanted biomaterials.
研究领域:
生物材料
·
生物医学工程
·
免疫学
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
npj Flexible Electronics
·
2024
·Vol. 8
·期 1
·1-12
·
IF 12.3
Ren Xueyang,Bai Wen,Chen Shisheng,Yuan Yuehui,Shao Xiaodong,Zhu Xuefei,Wang Li,Jiang Qin,Hu Benhui
Implantable neural probes, essential for brain electrophysiological research, have advanced with ultra-flexible designs to mitigate immune responses and postoperative complications. Strategies of shuttle-assisted implantation and temporary stiffening address issues in penetrating these probes into the target region, avoiding undesired bending. However, the risk of intraoperative bleeding remains due to these implants’ necessary rigidity during insertion. Here, we describe a neural probe with mechanical compliance accompanying self-implantation along the principal axis in the absence of bleeding. Crucial to the behavior is its anisotropic relaxation, which is dominated by the cross-sectional in-plane deformation inhibition due to interchain interactions between the parallel backbones in the globally aligned polymer system. We observed the ensured upright insertion of the probe into the brain while avoiding angiorrhexis with a two-photon microscope and a high-speed camera. The probes permit electrophysiological studies with minimal foreign body responses and imageological compatibility, underscoring their clinical potential.
研究领域:
神经科学
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生物医学工程
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材料科学
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产品关联
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-17
·
IF 14.7
Zhou Xianchi,Lu Zhouyu,Cao Wenzhong,Zhu Zihao,Chen Yifeng,Ni Yanwen,Liu Zuolong,Jia Fan,Ye Yang,Han Haijie,Yao Ke,Liu Weifeng,Wang Youxiang,Ji Jian,Zhang Peng
Polymeric elastomers are extensively employed to fabricate implantable medical devices. However, implantation of the elastomers can induce a strong immune rejection known as the foreign body response (FBR), diminishing their efficacy. Herein, we present a group of immunocompatible elastomers, termed easy-to-synthesize vinyl-based anti-FBR dense elastomers (EVADE). EVADE materials effectively suppress the inflammation and capsule formation in subcutaneous models of rodents and non-human primates for at least one year and two months, respectively. Implantation of EVADE materials significantly reduces the expression of inflammation-related proteins S100A8/A9 in adjacent tissues compared to polydimethylsiloxane. We also show that inhibition or knockout of S100A8/A9 leads to substantial attenuation of fibrosis in mice, suggesting a target for fibrosis inhibition. Continuous subcutaneous insulin infusion (CSII) catheters constructed from EVADE elastomers demonstrate significantly improved longevity and performance compared to commercial catheters. The EVADE materials reported here may enhance and extend function in various medical devices by resisting the local immune responses.
研究领域:
生物材料
·
生物医学工程
·
免疫学
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Liwei Sun,Xu Zhang,Jiteng Zhang,Xinmeng Li,Jie Zhao,Rujian Jiang,Lingjie Song,Shifang Luan
Hernia incidence remains a high level worldwide as witnessed by the fact that more than 20 million hernias are estimated to be repaired each year. Mesh-based hernioplasty allowing wound defects to close more easily and low recurrence rate is considered as the gold standard option for hernia repair. However, clinical polypropylene mesh (PPM) is still lack of effective treatments for the unavoidable bacterial infection, postoperative adhesion and foreign body reaction. Herein, a stimulus-responsive gel was constructed on the PPM by a simple photo-initiated polymerization. The homogeneous gel-modified mesh could spontaneously transform to a Janus mesh locally under endogenous stimulations evoked from inflammatory response or bacterial infection, and demonstrate asymmetric performances. One side of the Janus mesh facing the abdominal viscera could maintain intact gel structures and form a hydration layer to hinder the stick of proteins and cells, while the other side adjacent to the hernia defect site where bacteria prefer to colonize would accelerate degradation due to bacterial growth and release the bactericidal agent for combating the bacterial infection. The in vivo experiments’ results demonstrated that no adhesions were detectable on the Janus mesh treated group on the 14th day and the level of inflammatory response in histological evaluation was lower as compared to the commercial PPM. Overall, the endogenous stimulation-driven Janus gel network in abdominal wall wound provides a new approach for soft tissue repair biomaterials.
研究领域:
生物医学工程
·
材料科学
·
组织工程
·
外科材料
·
伤口愈合
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）