TALANTA
·
2023
·
IF 6.1
Feng Luo,Yue Tang,Jia Zheng,Zhengmin Xie,Junjun Wang,Jianli Zhou,Yuangen Wu
Carbendazim (CBZ) is a widely used pesticides, and its excessive intake is serious damage to humans and animals. Herein, a stable and sensitive colorimetric aptasensor for rapid detection of CBZ residue has been established based on the enhancement of CBZ-specific aptamer (CZ-13) on oxidase-mimicking activity of octahedral Ag 2 O nanoparticles (NPs). The CZ-13 aptamer can significantly increase the catalytic activity by promoting the production of superoxide anion (·O 2 − ) on the surface of Ag 2 O NPs and enhancing the affinity of octahedral Ag 2 O NPs to 3,3′,5,5′-tetramethylbenzidine (TMB) molecules. In the presence of CBZ, the quantity of CZ-13 aptamer will be exhausted due to the specific binding to CBZ pesticide. Thus, the rest CZ-13 aptamer no longer enhanced the catalytic activity of octahedral Ag 2 O NPs, which leads to the change in color of sensing solution. The color change of sensing solution can be easily converted to the corresponding RGB value by a smartphone for quantitative and rapid detection of CBZ. The designed aptasensor has excellent sensitivity and specificity, and the limit of detection was determined as low as 7.35 μg L −1 for CBZ assay. Besides, the aptasensor exhibited good recoveries in the spiked cabbage, apple and cucumber, showing that it may have broad application prospects for detecting CBZ residues in agriculture products.
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 50
·108774-108782
·
IF 5.8
Xu Jinjie,Li Yongli,Yu Lihong,Pang Yuehong,Shen Xiaofang,Liu Jun
In this work, metal–organic frameworks (MOFs) including Fe-MIL-101 and Ti-MIL-125 were prepared and fixed on the melamine foam (MF) by polyvinylidene fluoride (PVDF) to prepare MF/PVDF/MOFs, which was used as adsorbents in pipette-tip solid-phase extraction (PT-SPE) for rapid extraction of organophosphorus pesticides (OPPs). Then, a gas chromatograph-flame thermionic detector (GC-FTD) was used for simultaneous analysis of Dimethoate (DMT), Iprobenfos (IBF), Parathion-methyl (PAM), and Chlorpyrifos (CPF). The morphology, crystal structure, and functional groups of MF/PVDF/MOFs were characterized, indicating that Ti-MIL-125 and Fe-MIL-101 were successfully synthesized and distributed on MF. The Fe-MIL-101 and Ti-MIL-125 showed good extraction ability for OPPs, which was mainly due to the π-π interaction and the multiple porous structures. Under the optimal conditions, the limit of detection (LODs) of four OPPs was 0.03–0.14 μg L −1 and the RSDs were less than 9.9%. The developed PT-SPE method showed a short extraction time (<3 min). The recoveries in fruits and vegetables (Celery, cabbages, and oranges) ranged from 75.3%–118.8% (RSDs<9.6%). The prepared MF/PVDF/MOFs demonstrated the efficient extraction performance of OPPs, contributing to the rapid pretreatment of OPPs from food and the environment.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Linfeng Chen,Xike Tian,Yong Li,Liqiang Lu,Yulun Nie,Yanxin Wang
Accurate screening of organophosphorus and carbamates pesticides from the complex real sample is crucial for water quality analysis and food safety control. Herein, a simple, low-cost and accurate pesticides screening method based on a high-throughput optical array system assembled by multiple cholinesterases (ChE) and thiocholine (TCh) sensors is described. The detection mechanism is that the inhibition of ChE activity by pesticides reduces the TCh produced by the hydrolysis of butyryl/acetylthiocholine iodide, thus changing the fluorescence intensity of TCh sensor. The diverse response of ChEs to pesticides and different affinity of sensors to TCh ensure the high-throughput and distinguishable signal output, which allow the establishment of high discrete pesticide database with intra-cluster agglomeration and inter-cluster dispersion. By using the database, the screening of unknown real contaminated samples were successfully operated, and the screened pesticide species and concentrations were consistent with high-performance liquid chromatography. This screening strategy demonstrates the feasibility of replacing existing complex mass spectrometry-based screening strategy with simple optical analysis, providing a new idea for the development of simple accurate screening technologies for widespread organic pollutants including pesticides.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Linghui Huang,Yue Tang,Jianxun Han,Xiaojuan Niu,Xin Lin,Yuangen Wu
Malathion causes a serious threat to human health due to its widespread use in the environment. Herein, a novel and stable smartphone-integrated colorimetric biosensor for malathion detection is firstly established based on aptamer-enhanced laccase-mimicking activity. The results indicate that the M17-F aptamer can increase the affinity of Ag 2 O nanoparticles to the substrate 2,4-dichlorophenol and enhance their laccase-mimicking activity. Thus, abundant semiquinone radicals are produced in the catalytic system, which are combined with chromogenic agent to generate dark red products. The corresponding RGB values for the colour change of the solution can be easily obtained using smartphones, which is used for the rapid detection of malathion. The established biosensor for malathion has a limit of detection as low as 5.85 nmol·L −1 , and displays good selectivity for other competitive pesticides. Moreover, further studies have verified the applicability of the biosensor in actual samples, indicating that it may have the potential for application in malathion detection in food.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yan Zhou,Jinlong Wang,Wangni Meng,Lian Xia,Yue Tang,Jianli Zhou,Yuangen Wu,Pengcheng Yan
Acetamiprid (ACE), a neonicotinoid pesticide broadly used in agricultural production, can accumulate in the food chain due to excessive or incorrect use, thereby posing a threat to human health. In this manuscript , a colorimetric aptasensor assisted by a smartphone was established, relying on the S-18 aptamer-mediated amplification of the laccase-mimic activity of spherical Ag 2 O nanoparticles (NPs). The S-18 aptamer strengthens the interaction and facilitates electron transfer between the Ag 2 O NPs and oxidative substrates, which significantly improves the laccase-mimic activity of the Ag 2 O NPs. When acetamiprid is present, the S-18 aptamer preferentially binds to the target molecule, forming a steady aptamer-acetamiprid complex. This binding interaction prevents the aptamer from promoting the catalytic behavior of the Ag 2 O NPs, producing a distinct and quantifiable color variation in the reaction system. Monitoring the concentration-dependent colorimetric response enables the quick and accurate identification of acetamiprid. The colorimetric aptasensor achieved a limit of detection (LOD) of 8.69 μg∙L −1 markedly lower than the maximum residue limits defined by the national standard (GB 2763–2019) for acetamiprid in fruits and vegetables. Furthermore, the aptasensor demonstrates satisfactory recovery rates in actual samples analyses of cabbage, apple, and tomato, confirming its practical potential for on-site food safety oversight.
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高影响因子
危险品化学品经营许可证（带存储）