ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Haoyang Liu,Yue Zhu,Weixu Chen,Renwang Sheng,Chuanquan Liu,Yuzhi Sun,Jia Liu,Mingyue Wang,Jun Lu,Jialin Chen,Wei Zhang
The advancement of nanomedicine requires a thorough understanding of the intrinsic bioactivity and molecular interactions of nanomaterials for safe and effective clinical applications, which remains lacking for most currently developed nanomaterials. Here, we uncover the unique intrinsic bioactivity and regulatory mechanisms of carbon-based fullerol nanomaterials through high-throughput molecular analysis and explore their therapeutic potential for tissue regeneration using tissue engineering approaches. Fullerol exhibits intrinsic pro-differentiation and antioxidant properties that enhance the osteogenesis and chondrogenesis of MSCs. Mechanistically, proteomic analysis combined with small-molecule inhibition studies reveals that fullerol is internalized by MSCs via clathrin-mediated endocytosis and activates NRF2 signaling, thereby exerting antioxidant effects that restore impaired MSC viability and differentiation under oxidative stress. Leveraging these unique bioactivities, we develop a fullerol-functionalized hydrogel with feasible physicochemical properties and triple biological functions in antioxidant, pro-osteogenic, and pro-chondrogenic effects and confirm its great regenerative capacity for both cartilage and subchondral bone by promoting structural restoration and improving functional recovery in a rat osteochondral defect model. Our findings offer new insights into the intricate interactions between stem cells and nanomaterials at the cellular and molecular levels and broaden the potential biomedical applications of fullerol for future cartilage and bone regeneration therapies.
研究领域:
再生医学
·
纳米生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Catalysis
·
2024
·
IF 11.3
Xiaohui Leng,Xin Zhou,Lu Ma,Yonghua Du,Ouwen Peng,Zhongxin Chen,Jinhui Pan,Ming-Yu Qi,Jian-Hui Zheng,Yi-Jun Xu,Kian Ping Loh
Visible light induced oxidative C–C and C–N coupling provides a milder alternative to the current synthetic strategies. However, the photochemical C–C coupling of benzylamine and chromoselective C–C/C–N conversion are not widely reported. Herein, we demonstrate that the introduction of indium vacancies in ZnIn2S4 improves the catalytic activity for C–C coupling of benzylamine and imparts a strong chromoselectivity for C–C or C–N coupled products. A high C–C selectivity of ∼80% is achieved with a benzylamine conversion of ∼80%. Mechanistic studies unveil the switchable reaction routes between kinetically favored C–C coupling and thermodynamically favored C–N coupling under specific illumination conditions.
研究领域:
化学
·
材料科学
·
光催化
✓ 已验证
产品关联
DOI
高影响因子
TETRAHEDRON
·
2020
·
IF 2.233
Zhen-Hua Li,Xiao-Meng Sun,Jin-Jing Qin,Zhi-Yong Tan,Wen-Biao Wang,Yao Ma
An efficient and divergent synthesis of N -arylbenzimidazoles and N -arylindazoles from arylamino oximes based on reaction conditions selection was developed. The synthesis was approached by treating oximes with BTC and the chemoselectivity was regulated by the amount of Et 3 N. This switchable N–N and N–C bond formation process features mild reaction conditions, simple execution, high chemoselectivity and broad substrate scope.
研究领域:
有机化学
·
合成化学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Lingbo Sun,Hongxia Fu,Yanru Li,Xinrui Duan,Zhengping Li
New approach for colon cancer stem cells (CSCs) recognition and isolation is reported. Colon CSCs are responsible for colonic tumor growth, metastasis, and resistance for radio-/chemotherapies. An accurate identification and isolation method is critical for understanding and characterization of these cells. In our work, we recognized CSCs’ population from colon cancer cells by using metabolic labeling of azido sugar based on the quiescent nature of these cells, which differed fundamentally from previously described methods by using specific cellular markers to recognize and isolate CSCs. Later the putative CSCs were isolated by using commercially available magnetic beads. The isolated cells population had much higher sphere formation efficiency, soft-agar colony formation efficiency, and an mRNA level of colon stem cells marker Lgr5 than the leftover population. Our method provides a new avenue and a general strategy for recognition and isolation of CSCs, which shows great potential for further use in both the fundamental research of CSCs and clinical tests.
研究领域:
肿瘤学
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细胞生物学
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癌症研究
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干细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2024
·Vol. 12
·期 19
·11424-11438
·
IF 10.7
Siyuan Chen,Yanhong Wang,Shuanshi Fan,Xuemei Lang,Gang Li
Hydrate-based hydrogen storage is considered one of the most promising hydrogen storage technologies, but its slow formation kinetics impedes its applicability. In this study, a novel method to enhance hydrate nucleation and hydrate growth was proposed. Ultra-rapid and high hydrogen storage was achieved by the new method of quasi-simultaneous formation (q-SF) of propane–hydrogen hydrates. And different kinetic hydrate additives (KHAs) were introduced to enhance the kinetics of hydrate formation. The results demonstrated that this method implemented the transition of progressive nucleation to instantaneous nucleation of hydrogen hydrate, with the enhancing growth kinetics of hydrate. In the study of KHAs, sodium dodecyl sulfate (SDS) had a significant promoting effect on propane–hydrogen hydrate growth, and high-concentration SDS could achieve higher water-to-hydrate conversion. The maximum hydrogen uptake was 67.50 cm3 cm−3 hydrate (0.628 wt%) at 274.15 K and the initial pressure of 18.2 MPa with a system of 0.1 wt% SDS coupled with stirring. The time from hydrate nucleation to the maximum hydrogen uptake of 90% (t90) was only 21 min, and the average hydrate growth rate was 289.28 × 10−2 cm3 cm−3 hydrate·min−1. As far as we know, this is the fastest hydrate growth rate among hydrate-based hydrogen storage technologies so far. A mechanism was proposed to explain the promoting effect of q-SF on hydrate formation kinetics. This work provides unique insights into enhancing the kinetics of hydrogen hydrates, which would contribute to the further development of hydrate-based hydrogen storage technologies.
研究领域:
化学工程
·
能源技术
✓ 已验证
DOI
高影响因子
MONATSHEFTE FUR CHEMIE
·
2014
·Vol. 145
·期 12
·1925-1931
·
IF 1.347
Tu Xingzhao,Zhou Lihong,Li Zhijie,Lei Ning,Zeng Qingle
Abstract The carbon-heteroatom bond formation is an important research field. Transition-metal-free synthesis of medicinally important heterocycles avoids products of transition metal contamination, and thus it is an environmentally friendly and cost-saving process. A transition-metal-free domino C-S/C-N formation for the synthesis of imidazobenzothiazines from 2-mercaptobenzimidazoles and 2-halobenzyl bromides is developed. The desired products were obtained in good to excellent yields. The mechanism of domino nucleophilic substitution (S N 2) and nucleophilic aromatic substitution (S N Ar) is proposed. Graphical abstract
研究领域:
有机化学
·
药物化学
·
绿色化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2024
·
IF 3.9
Xuemeng Ji,Dancai Fan,Jin Wang,Bowei Zhang,Yaozhong Hu,Huan Lv,Jing Wu,Yi Sun,Jingmin Liu,Yan Zhang,Shuo Wang
Cronobacter sakazakii poses a significant threat, particularly to neonates and infants. Despite its strong pathogenicity, understanding of C. sakazakii biofilms and their role in infections remains limited. This study investigates the roles of HmsP and c-di-GMP in biofilm formation and identifies key genetic and proteomic elements involved. Gene knockout experiments reveal that HmsP and c-di-GMP are linked to biofilm formation in C. sakazakii. Comparative proteomic profiling identifies the lysozyme inhibitor protein LprI, which is downregulated in hmsP knockouts and upregulated in c-di-GMP knockouts, as a potential biofilm formation factor. Further investigation of the lprI knockout strain shows significantly reduced biofilm formation and decreased virulence in a rat infection model. Additionally, LprI is demonstrated to bind extracellular DNA, suggesting a role in anchoring C. sakazakii within the biofilm matrix. These findings enhance our understanding of the molecular mechanisms underlying biofilm formation and virulence in C. sakazakii, offering potential targets for therapeutic intervention and food production settings.
研究领域:
微生物学
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分子生物学
·
感染病学
✓ 已验证
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Detao Li,Zichen Ning,Zhuoshan Gong,Limin Zhou,Li Xu,Feiqiang He,Hamza Shehzad,Heng Jerry,Shichao Du,Jinbo Ouyang
According to in-depth research on cocrystals of polyhydroxylated natural products, we previously summarized the substitution model to elucidate the formation mechanism of cocrystals. However, when refers to salt formation of natural products, the substitution model reveals numerous deficiencies. To compensate for the shortcomings of substitution model in predicting salt formation, this study conducts a comprehensive exploration of natural product salts. By supplementing the energy difference rule to the substitution model, it endows the modified model with broader applicability. Simultaneously, the transfer of protons within salts structures is verified by this modified model. Furthermore, we apply competitive cocrystallization technique to separate flavonoid natural products (quercetin, hesperetin, and naringenin). In view of the characteristics of various natural products as well as their cocrystals/salts, numerous separation processes are developed, and the separation effects of these processes are analyzed in detail. The purity of the products obtained from separation process all reaches above 70%, strongly demonstrating the feasibility of using cocrystallization for the binary separation of flavonoids. This work not only refines the substitution model for predicting cocrystals/salts formation of natural products but also indicates the promising application prospects of cocrystallization in industrial separation of natural products.
研究领域:
有机化学
·
药学
·
分离工程
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR BIOLOGY REPORTS
·
2024
·Vol. 51
·期 1
·1-12
·
IF 2.6
Zheng Ruzhen,He Yibo,Yang Lingrong,Chen Yidan,Wang Rui,Xie Shangnao
Background Our previous study has demonstrated that Nischarin (NISCH) exerts its antitumor effects in breast cancer (BC) by suppressing cell migration and invasion. This study aims to explore the underlying mechanism through which NISCH functions in BC. Methods and results The relevance between EGF Like Repeats and Discoidin Domains 3 (EDIL3) mRNA expression and the overall survival of tumor patients was depicted by the Kaplan-Meier curve. The findings revealed that overexpressed NISCH attenuated cell motility and colony-forming capacities of Hs578T cells, yet silenced NISCH in MDA-MB-231 cells led to contrasting results. Western blot (WB) analysis indicated that overexpression of NISCH significantly down-regulated the Vimentin and Slug expression, and inactivated the FAK/ERK signaling pathway. RNA sequencing (RNA-seq) was performed in NISCH-overexpressed Hs578T cells and the control cells to analyze differentially expressed genes (DeGs), and the results showed a significant down-regulation of EDIL3 mRNA level upon overexpression of NISCH. Subsequent functional analyses demonstrated that overexpression of EDIL3 attenuated the inhibitory effect of NISCH on cell migration, invasion, colony formation, and tube formation. Conclusion In summary, our finding preliminarily revealed that NISCH inhibits the epithelial-mesenchymal transition (EMT) process and angiogenesis in BC cells by down-regulating EDIL3 to inactivate the FAK/ERK signaling pathway, thereby suppressing the progression of BC. Our results hold promise for contributing to the deep understanding of BC pathogenesis and identifying new therapeutic strategies for clinical application.
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
高影响因子
Environmental Science-Water Research & Technology
·
2020
·Vol. 6
·期 10
·2766-2775
·
IF 3.449
Huan Qi,Qing Lin,Mengyi Chen,Xiaobin Liao,Jie Chen,Fei Li,Baoling Yuan
Daminozide (DMNZD) has been found to be an important ozone-reactive N-nitrosodimethylamine (NDMA) precursor; however, the influence of humic acid (HA) on NDMA formation from daminozide during ozonation is unclear. In this study, the impacts of HA on NDMA formation from daminozide at various oxidation conditions (ozone dosages, pH) and other water components (bicarbonate ion (HCO3−), sulfate ion (SO42−)) were investigated. In addition, its implications for water treatment plants are discussed. The results indicated that low levels of HA (≤0.6 mg L−1) facilitated NDMA formation and its molar yield was maximized at 0.1 mg L−1 HA (8.5%), while a high concentration of HA (>0.6 mg L−1) had an inhibition role. The NDMA amount increased with increasing pH and showed a peak at pH 8. With 0.1 mg L−1 HA, NDMA was raised from 3.0% to 23.1% with ozone increasing from 0.5 to 4 mg L−1; HCO3− competed with daminozide for ozone and cut down NDMA formation, whereby 160 μM HCO3− reduced NDMA conversion from 8.5% to 6.5%; however, with the increase in SO42− from 0 to 20 μM, NDMA conversion was augmented from 8.5% to 17.8%. Based on the results of LC/MS/MS and GC/MS analyses, NDMA transformation pathways with/without HA are proposed.
研究领域:
环境科学
·
化学
✓ 已验证
DOI
高影响因子
POLYMER
·
2024
·
IF 4.1
Miaomiao Xue,Zhenzhen Quan,Xiaohong Qin,Jianyong Yu,Yuling Li
Numerous experimental data indicate that higher viscosity solutions make it easier to obtain coarser fibers. However, the influences of viscosity on the whipping behavior of the jet and their subsequent impacts on the formation of terminal fiber during electrospinning remain topics of ongoing research. Simulation model and experiment presented in this study aim to solve these problems. The simulation model alters the dimensionless parameter F ve to predict the impacts of different viscosities on the bending behavior of the electrospun jet, while the experiment observes this behavior under different viscosities by regulating the solution concentration. The consistency of the simulation and experimental data implies the accuracy of the conclusions in this paper. Furthermore, the simulation model and experiment have demonstrated that a higher viscosity jet results in a smaller radius of the jet trajectory and lower jet velocity, which in turn leads to the formation of coarser fiber.
研究领域:
材料科学
·
物理学
✓ 已验证
产品关联
DOI
高影响因子
Cell Death & Disease
·
2025
·Vol. 16
·期 1
·1-11
·
IF 9.6
Chu Xiufeng,Zhang Ting,Bukhari Ihtisham,Hu Mei,Xu Jixuan,Xing Yamin,Liang Xinfeng,Zhang Zisen,Zheng Pengyuan
Gasdermin D (GSDMD) is a critical pyroptosis mediator, consisting of one N-terminal pore-forming domain and one C-terminal auto-inhibitory domain. The free N-terminal domain (GD-NT), which is released through caspase-1/11 cleavage, exhibits distinct features from the full-length GSDMD (GD-FL), including oligomerization, membrane translocation, and pore-formation. However, the underlying mechanisms are not well elucidated. Here, we found that GD-NT, but not GD-FL, was massively ubiquitinated in cells. The K63-linked polyubiquitination of GD-NT at Lys236/237 (human/mouse), catalyzed by TRAF1, directly prompted its membrane translocation and pore-formation during pyroptosis. Inhibition of GD-NT ubiquitination via site-directed mutations or the UBA1 inhibitor PYR-41 suppressed cell death in several pyroptosis cell models. Additionally, applying PYR-41 in septic mice efficiently suppressed the release of IL-18 and TNFα. Thus, GD-NT ubiquitination is a key regulatory mechanism controlling its membrane localization and activation, which may provide a novel target for modulating immune activity in pyroptosis-related diseases.
研究领域:
免疫学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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